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| 1 | 山东省局部地区番茄黄化曲叶病毒的分子鉴定显示文摘近年来山东省部分地区发现番茄黄化曲叶症状。为了确定这种疑似病毒,利用双生病毒外壳蛋白(CP)基因及DNA-A基因组的引物对枣庄、菏泽和淄博的染病番茄叶片进行了检测,测序分析表明这种病毒是番茄黄化曲叶病毒(TYLCV)。系统发育分析结果表明,该病毒可能是由我国东南沿海地区和日本传入的。 | 褚栋 侯丽霞 刘国霞 高长生 Judith K Brown | 2010 | 山东农业科学2010，42，2： | 24 |
| 2 | Autonomic and endocrine control of cardiovascular function显示文摘The function of the heart is to contract and pump oxygenated blood to the body and deoxygenated blood to the lungs.To achieve this goal,a normal human heart must beat regularly and continuously for one's entire life.Heartbeats originate from the rhythmic pacing discharge from the sinoatrial(SA) node within the heart itself.In the absence of extrinsic neural or hormonal influences,the SA node pacing rate would be about 100 beats per minute.Heart rate and cardiac output,however,must vary in response to the needs of the body's cells for oxygen and nutrients under varying conditions.In order to respond rapidly to the changing requirements of the body's tissues,the heart rate and contractility are regulated by the nervous system,hormones,and other factors.Here we review how the cardiovascular system is controlled and influenced by not only a unique intrinsic system,but is also heavily influenced by the autonomic nervous system as well as the endocrine system. | Richard Gordan Judith K Gwathmey Lai-Hua Xie | 2015 | World Journal of Cardiology2015，7，4： | 7 |
| 3 | 乳腺癌发生模型MCF10各细胞系中APC基因启动子区甲基化及mRNA表达检测显示文摘目的探讨乳腺癌发生模型MCF10中抑癌基因APC启动子区甲基化状态及其对mRNA表达的影响。方法应用甲基化特异性聚合酶链反应(MSP)及双亚硫酸钠基因测序技术检测MCF10模型的乳腺增生细胞系MCF10A、癌前细胞系MCF10AT、导管内癌细胞系MCF10DC IS.com、浸润癌细胞系MCF10CA1 a、MCF10CA1d、MCF10CA1h及经典乳腺癌细胞系MCF-7和正常乳腺组织中APC基因启动子1A甲基化状态,然后用逆转录聚合酶链反应(RT-PCR)和实时PCR技术检测上述样品的mRNA表达水平。结果在MCF10模型的增生细胞系、癌前细胞系、导管内癌细胞系、浸润癌细胞系中,APC基因启动子1A处于低甲基化状态;与正常乳腺组织相比,各细胞系APC基因mRNA表达无明显减少,MCF10AT、MCF10CA1d、MCF10CA1h、MCF10DC IS.com的mRNA表达分别减少0.27、0.96、1.78、2.70、2.03倍,MCF10A和MCF-7分别增加0.02和0.33倍)。结论MCF10模型中乳腺癌的发生发展过程与APC基因启动子区异常甲基化无关。 | 杨举伦 David Klinkebiel Michael J Boland Lin Tang Judith K Christman | 2006 | 中华病理学杂志2006，35，1： | 7 |
| 4 | 乳腺癌发生模型MCF10各阶段细胞系中TIMP3基因启动子区甲基化分析显示文摘目的探讨抑癌基因TIMP3失活与乳腺癌发生和进展的关系。方法用甲基化特异性PCR技术和亚硫酸盐测序技术检测乳腺癌发生模型MCF10的增生细胞系MCF10A、癌前细胞系MCF10AT、导管内癌细胞系MCF10DCIS.com、浸润癌细胞系MCF10CA1a和转移癌细胞系MCF10CA1d、MCF10CA1h中TIMP3启动子区甲基化状态。结果甲基化特异性PCR分析显示,在上述各细胞系中,TIMP3启动子区均呈高度甲基化状态。亚硫酸盐测序显示,在上述各细胞系中,测序区内的68个CG位点几乎全部发生了甲基化,且甲基化累及了绝大部分等位基因。结论TIMP3基因启动子区甲基化在乳腺癌的发生和进展中起重要作用,可能成为早期诊断乳腺癌和判断乳腺癌预后的分子生物学标记。 | 杨举伦 David Klinkebiel Michael J Boland Lin Tang Judith K Christman | 2006 | 临床与实验病理学杂志2006，22，1： | 7 |
| 5 | Effects of host, temperature and relative humidity on competitive displacement of two invasive Bemisia tabaci biotypes [Q and B]显示文摘Bemisia tabaci 在 20052008 期间从 B 遗传因子型的优势在中国出人意料地变了到 Q 遗传因子型。这观察在调查环境因素,包括的主人,温度和相对湿度(RH ) 是否能可能在遗传因子型分发解释观察移动刺激了兴趣。结果显示所有三个参数检验了影响遗传因子型 survivability。当与 Q 遗传因子型在辣椒植物上一起高耸了时, B 遗传因子型的百分比在第一和第二代在创始人人口从 66.7% 显著地减少了,到 13.6% 和 3.7% 分别地。什么时候 B (在 66.7% 点的创始人) 和 Q (在 33.3% 点的创始人) 遗传因子型独自在茄子上,或在 pepper-plus-eggplant 联合上一起被饲养, B 遗传因子型任何一个的人口尺寸仍然保持不变,或在第一和第二代有点增加了。在茄子上,在 Q 和 B 遗传因子型(3 Q 和 6 B ) 之间的竞争力上的 RH 和温度的效果不是重要的。 | Dong Chu Yun-Li Tao You-Jun Zhang Fang-Hao Wan Judith K Brown | 2012 | Insect Science2012，19，5： | 7 |
| 6 | 乳腺癌发生过程中NOEY2基因启动子区甲基化及mRNA表达显示文摘目的探讨乳腺癌发生过程中抑癌基因NOEY2启动子区甲基化状态及其对mRNA表达的影响。方法应用甲基化特异性PCR及双亚硫酸钠基因测序技术检测MCF10模型中乳腺增生细胞系MCF10A、癌前细胞系MCF10AT、导管内癌细胞系MCF10DC IS.com、浸润癌细胞系MCF10CA1 a、MCF10CA1d、MCF10CA1h及正常乳腺组织中NOEY2基因启动子区CpG岛I甲基化状态,然后用RT-PCR和实时PCR技术检测上述样品的mRNA表达水平。结果MCF10模型的增生细胞系、癌前细胞系、导管内癌细胞系、浸润癌细胞系均发生该基因启动子区CpG岛I高度甲基化;与正常乳腺组织相比,上述细胞系mRNA表达显著减少。结论NOEY2基因启动子区高度甲基化及相应的mRNA表达减少是乳腺癌发生过程中的早期事件,与乳腺癌发生有关,可能成为早期诊断乳腺癌的潜在分子生物学标记。 | 杨举伦 David Klinkebiel Michael J Boland Lin Tang Judith K Christman | 2005 | 临床与实验病理学杂志2005，21，5： | 6 |
| 7 | Oxidative stress-mediated effects of angiotensin Ⅱ in the cardiovascular system显示文摘Angiotensin Ⅱ(Ang Ⅱ), an endogenous peptide hormone, plays critical roles in the pathophysiological modulation of cardiovascular functions. Ang Ⅱ is the principle effector of the renin-angiotensin system for maintaining homeostasis in the cardiovascular system, as well as a potent stimulator of NAD(P)H oxidase, which is the major source and primary trigger for reactive oxygen species(ROS) generation in various tissues. Recent accumulating evidence has demonstrated the importance of oxidative stress in Ang Ⅱ-induced heart diseases. Here, we review the recent progress in the study on oxidative stress-mediated effects of Ang Ⅱ in the cardiovascular system. In particular, the involvement of Ang Ⅱ-induced ROS generation in arrhythmias, cell death/heart failure, ischemia/reperfusion injury, cardiac hypertrophy and hypertension are discussed. Ca2+/calmodulin-dependent protein kinase Ⅱ is an important molecule linking Ang Ⅱ, ROS and cardiovascular pathological conditions. | Hairuo Wen Judith K Gwathmey Lai-Hua Xie | 2012 | World Journal of Hypertension2012，2，4： | 3 |
| 8 | 乳腺癌发生模型MCF10抑癌基因NOEY2启动子区甲基化及mRNA表达显示文摘 | 杨举伦 David Klinkebiel Michael J Boland Lin Tang Judith K Christman | 2005 | 中华病理学杂志2005，34，3： | 3 |
| 9 | Effect of process parameters on additive-free electrospinning of regenerated silk fibroin nonwovens显示文摘Silk fibroin is a biomaterial with multiple beneficial properties for use in regenerative medicine and tissue engineering.When dissolving and processing the reconstituted silk fibroin solution by electrospinning,the arrangement and size of fibers can be manifold varied and according fiber diameters reduced to the nanometer range.Such nonwovens show high porosity as well as potential biocompatibility.Usually,electrospinning of most biomaterials demands for the application of additives,which enable stable electrospinning by adjusting viscosity,and are intended to evaporate during processing or to be washed out afterwards.However,the use of such additives increases costs and has to be taken into account in terms of biological risks when used for biomedical applications.In this study,we explored the possibilities of additive-free electrospinning of pure fibroin nonwovens and tried to optimize process parameters to enable stable processing.We used natural silk derived from the mulberry silkworm Bombyx mori.After degumming,the silk fibroin was dissolved and the viscosity of the spinning solution was controlled by partial evaporation of the initial solving agent.This way,we were able to completely avoid the use of additives and manufacture nonwovens,which potentially offer higher biocompatibility and reduced immunogenicity.Temperature and relative humidity during electrospinning were systematically varied(25–35°C,25–30%RH).In a second step,the nonwovens optionally underwent methanol treatment to initiate beta-sheet formation in order to increase structural integrity and strength.Comprehensive surface analysis on the different nonwovens was performed using scanning electron microscopy and supplemented by additional mechanical testing.Cytotoxicity was evaluated using BrdU-assay,XTT-assay,LDH-assay and live-dead staining.Our findings were,that an increase of temperature and relative humidity led to unequal fiber diameters and defective nonwovens.Resistance to penetration decreased accordingly.The most uniform fiber diameters of 998±63 nm were obtained at 30°C and 25%relative humidity,also showing the highest value for resistance to penetration(0.20 N).The according pure fibroin nonwoven also showed no signs of cytotoxicity.However,while the biological response showed statistical evidence,the material characteristics showed no statistically significant correlation to changes of the ambient conditions within the investigated ranges.We suggest that further experiments should explore additional ranges for temperature and humidity and further focus on the repeatability of material properties in dependency of suitable process windows. | Alexander Kopp Ralf Smeets Martin Gosau Nadja Kröger Sandra Fuest Marius Köpf Magnus Kruse Judith Krieger Rico Rutkowski Anders Henningsen Simon Burg | 2020 | Bioactive Materials2020，5，2： | 3 |
| 10 | 制药企业药品上市前安全风险管理显示文摘1药物警戒的必要性良好的药物警戒是最理想的药品风险管理方式,它可以最大程度的了解某种药品的所有信息,并且发现它潜在的安全问题,甚至有可能发现其预料之外的好处。鉴于这个理念,新化学实体(newchemical entity)在研发初期就必须进行动物和人体安全性测试,以便更好地促进并保护公众健康。中国古话所说的"是药三分毒", | Judith K Jones Elyse E Kingery Jing Tao Jeff J Guo | 2013 | 药物流行病学杂志2013，22，5： | 2 |
| 11 | Translating the A1c assay into estimated average glucose values 显示文摘 | David M Judith K Rikke B | 2008 | Diabetes Care2008，31，： | 1 |
| 12 | Chilling injury inmango fruit peel : Cultivar differences are related to the activityof phenylalanine ammonia lyase 显示文摘 | Sugunya C Saichol K Judith B | 2011 | Postharvest Biology andTechnology2011，62，： | 1 |
| 13 | Recovery of polyphenols from the by-products of plant food processing and application as valuable food ingredients显示文摘 | DIETMAR R K JUDITH K REGINE V | 2014 | Food Research International2014，，： | 1 |
| 14 | Gemini virus-mediated genesilencing firom cotton leaf crumple virus is enhanced by low tempera-ture in cotton显示文摘 | John R T Idris A M Judith K B | 2008 | Plant Physiology2008，1148，： | 1 |
| 15 | Adsorption of phenols from aqueous solutions using mesoporous carbon prepared by two - stage process 显示文摘 | L John Kennedy J Judith Vijaya K Kayalvizhi etal | 2007 | Chemical Engineering Journal2007，132，： | 1 |
| 16 | Gastric Banding or Bypass? A Systematic Review Comparing the Two Most Popular Bariatric Procedures显示文摘 | Jeffrey AT Leah K Judith W | 2008 | Am J Med2008，121，10： | 1 |
| 17 | Role of glutamine in immunologic responses显示文摘 | Douglas W Wilmore M D Judith K | 1998 | Nutr1998，14，： | 1 |
| 18 | Mim- icking live flavivirus immunization with a noninfectious RNA vac- cine显示文摘 | REGINA M K JUDITH H A STEPHAN W A | 2014 | Proc Natal Acad Sci USA2014，101，7： | 1 |
| 19 | Mir451 regulates zebrafish erythroid maturation in vivo via its target gata2显示文摘 | LUKE P JUDITH E L WIGARD P K | 2009 | Blood2009，113，： | 1 |
| 20 | Physico-chemical stability of docetaxel premix solution and docetaxel infusion solutions in PVC bags and polyolefine containers显示文摘 | Judith T Irene K | 1999 | Pharm World sci1999，21，3： | 1 |
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