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| 1 | Probiotic Leuconostoc mesenteroides ssp. cremoris and Streptococcus thermophilus induce IL-12 and IFN-γ production显示文摘AIM: To investigate the capacity of potentially probiotic strains from six bacterial genera to induce cytokine production alone or in combinations in order to identify potential enhancing or synergistic effects in order to select probiotic bacteria for in vivo purposes. METHODS: Cytokine production in human peripheral blood mononuclear cells (PBMC) in response to stimulation with eleven different potentially probiotic bacterial strains from Streptococcus , Lactobacillus , Bifidobacterium , Lactococcus , Leuconostoc and Propionibacterium genera was analysed. Production and mRNA expression of TNF-α, IL-12, IFN-γ and IL-10 were determined by ELISA and Northern blotting, respectively. RESULTS: All tested bacteria induced TNF-α production. The best inducers of Th1 type cytokines IL-12 and IFN-γ were Streptococcus and Leuconostoc strains. All Bifidobacterium and Propionibacterium strains induced higher IL-10 production than other studied bacteria. Stimulation of PBMC with any bacterial combinations did not result in enhanced cytokine production suggesting that different bacteria whether gram-positive or gram- negative compete with each other during host cell interactions.CONCLUSION: The probiotic S. thermophilus and Leuconostoc strains are more potent inducers of Th1 type cytokines IL-12 and IFN-γ than the probiotic Lactobacillus strains. Bacterial combinations did not result in enhanced cytokine production. | Riina A Kekkonen Elina Kajasto Minja Miettinen Ville Veckman Riitta Korpela Ilkka Julkunen | 2008 | World Journal of Gastroenterology2008，14，8： | 9 |
| 2 | Potentially probiotic bacteria induce efficient maturation but differential cytokine production in human monocyte-derived dendritic cells显示文摘AIM: To analyze the ability of nine different potentially probiotic bacteria to induce maturation and cytokine production in human monocyte-derived dendritic cells (moDCs). METHODS: Cytokine production and maturation of moDCs in response to bacterial stimulation was analyzed with enzyme-linked immunosorbent assay (ELISA) and flow cytometric analysis (FACS), respectively. The kinetics of mRNA expression of cytokine genes was determined by Northern blotting. The involvement of different signaling pathways in cytokine gene expression was studied using specific pharmacological signaling inhibitors. RESULTS: All studied bacteria induced the maturation of moDCs in a dose-dependent manner. More detailed analysis with S. thermophilus THS, B. breve Bb99, and L. lactis subsp. cremoris ARH74 indicated that these bacteria induced the expression of moDC maturationmarkers HLA class Ⅱ and CD86 as efficiently as pathogenic bacteria. However, these bacteria differed in their ability to induce moDC cytokine gene expression. S. thermophilus induced the expression of pro-inflammatory (TNF-α, IL-12, IL-6, and CCL20) and Th1 type (IL-12 and IFN-γ) cytokines, while B. breve and L. lactis were also potent inducers of anti- inflammatory IL-10. Mitogen-activated protein kinase (MAPK) p38, phosphatidylinositol 3 (PI3) kinase, and nuclear factor-kappa B (NF-κB) signaling pathways were shown to be involved in bacteria-induced cytokine production. CONCLUSION: Our results indicate that potentially probiotic bacteria are able to induce moDC maturation, but their ability to induce cytokine gene expression varies significantly from one bacterial strain to another. | Sinikka Latvala Taija E Pietil Ville Veckman Riina A Kekkonen Soile Tynkkynen Riitta Korpela Ilkka Julkunen | 2008 | World Journal of Gastroenterology2008，14，36： | 8 |
| 3 | Probiotic intervention has strain-specific anti-inflammatory effects in healthy adults显示文摘AIM: To evaluate the effects of three potentially anti- inflammatory probiotic bacteria from three different genera on immune variables in healthy adults in a clinical setting based on previous in vitro characterization of cytokine responses. METHODS: A total of 62 volunteers participated in this randomized, double-blind and placebo-controlled parallel group intervention study. The volunteers were randomized to receive a milk-based drink containing either Lactobacillus rhamnosus GG (LGG), Bifidobacterium animalis ssp. lactis Bb12 (Bb12), or Propionibacterium freudenreichii ssp. shermanii JS (PJS) or a placebo drink for 3 wk. Venous blood and saliva samples were taken at baseline and on d 1, 7 and 21. Fecal samples were collected at baseline and at the end of intervention. RESULTS: The serum hsCRP expressed as the median AUC0-21 (minus baseline) was 0.018 mg/L in the placebo group, -0.240 mg/L in the LGG group, 0.090 mg/L in the Bb12 group and -0.085 mg/L in the PJS group (P = 0.014). In vitro production of TNF-α from in vitro cultured peripheral blood mononuclear cells (PBMC) was significantly lower in subjects receiving LGG vs placebo. IL-2 production from PBMC in the Bb12 group was significantly lower compared with the other groups. CONCLUSION: In conclusion, probiotic bacteria have strain-specific anti-inflammatory effects in healthy adults. | Riina A Kekkonen Netta Lummela Heli Karjalainen Sinikka Latvala Soile Tynkkynen Salme Jrvenp Hannu Kautiainen Ilkka Julkunen Heikki Vapaatalo Riitta Korpela | 2008 | World Journal of Gastroenterology2008，14，13： | 6 |
| 4 | Interferons up-regulate STAT1, STAT2, and IRF family transcription factor gene expression in human peripheral blood mononuclear cells and macrophages显示文摘 | Lehtonen A Matikainen S Julkunen I | 1997 | J Immunol1997，159，2： | 1 |
| 5 | Comparison of single-phase isotropic elastic and fibril-reinforced poroelastic models for indentation of rabbit articular cartilage显示文摘 | Julkunen P Harjula T Marjanen J | 2009 | J Biomech2009，42，5： | 1 |
| 6 | Nonrenal and renal activity of systemic lupus erythematosus:a comparison of two anti-C1q and five anti-dsDNA assays and complement C3 and C4显示文摘 | Julkunen H Ekblom-Kullberg S Miettinen A | 2012 | Rheumatol Int2012，32，： | 1 |
| 7 | IFN-α regulates Toll-like receptor -mediated IL-27 gene expression in human macrophages显示文摘 | Pirhonen J Siren J Julkunen I | | 0，，05： | 1 |
| 8 | Complete amino acid sequence of human placental protein 14: a progesterone- regulated uterine protein homologous to β-lactoglobulins 显示文摘 | Julkunen M Seppala M Janne O | 2006 | Proc Natl Acadsci USA2006，104，12： | 1 |
| 9 | Recovery of somatosen-sory deficits in acute stroke显示文摘 | Julkunen L Tenovuo O Jaaskelainen SK | 2005 | Acta Neurol Scand2005，111，6： | 1 |
| 10 | Interaction of viral envelope glycoproteins with fibronectin显示文摘 | Julkunen I Hautanen A Keski-Oja J | 1983 | Infect Immun1983，40，3： | 1 |
| 11 | Localization of viral-envelope-glycoprotein-binding sites in fibronectin显示文摘 | Julkunen I Vartio T Keski-Oja J | 1984 | Biochem J1984，219，2： | 1 |
| 12 | Hope in nursing research: A meta-analysis of the ontological and epistemological foundations of research on hope显示文摘 | Kylma J Vehvilainen Julkunen K | 1997 | J Adv Nurs1997，25，2： | 1 |
| 13 | Fibronectin and viral pathogenesis显示文摘 | Keski-Oja J Hautanen A Julkunen I | 1987 | Rev Infect Dis1987，9，4： | 1 |
| 14 | Multiple signaling pathways contribute to synergistic TLR ligand-dependent cytokine gene expression in human monocyte- derived macrophages and dendritic cells显示文摘 | Makela SM Strengell M Pietila TE Osterlund P Julkunen I | 2009 | J Leukoc Biol2009，85，4： | 1 |
| 15 | Effects of low-frequency magnetic fields on fetal development in CBA/Ca mice显示文摘 | Huuskonen H Juutilainen J Julkunen A | 1998 | Bioelectromagnetics1998，19，8： | 1 |
| 16 | Changes in transformational leadership and empirical quality outcomes in a finnish hospital over a two- year period: a longitudinal study显示文摘 | M/intynen R Vehvil/iinen - Julkunen K Partanen P | 2014 | Nurs Res Pract2014，2014，： | 1 |
| 17 | The NS1 Protein of Influenza A Virus Blocks RIG-I-Mediated Activation of the Noncanonical NF-κB Pathway and p52/RelB-Dependent Gene Expression in Lung Epithelial Cells显示文摘 | Andrea Rückle Emanuel Haasbach Ilkka Julkunen Oliver Planz Christina Ehrhardt Stephan Ludwig | 2012 | Journal of Virology2012，，18： | 1 |
| 18 | Molecular pathogenesis of influenza A virus infection and virusinduced regulation of cytokine gene expression显示文摘 | Julkunen I Sareneva T Pirhonen J | 2001 | Cytokine Growth Factor Rev2001，12，2： | 1 |
| 19 | Multi-method analysis of MRI images in early diagnostics of Alzheimer's disease显示文摘 | Wolz R Julkunen V Koikkalainen J | | 0，，10： | 1 |
| 20 | alpha-,beta-and chiβ-catenin expression in human pancreatic cancer显示文摘 | Julkunen K Makinen K Karja V | 2003 | Anticancer Res2003，23，6： | 1 |
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