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| 1 | Role of Ki-67 as a prognostic factor in gastrointestinal stromal tumors显示文摘AIM:To investigate primarily the prognostic value of Ki-67,as well as other parameters,in gastrointestinal stromal tumors(GISTs).METHODS:Ki-67,c-KIT,platelet-derived growth factor receptor-alpha(PDGFRα),smooth muscle actin(SMA),CD34,S100 were stained for immunohistochemistry which was performed on formalin-fixed,paraffinembeded sections on representative block from each case.Proliferation index counted by Ki-67 antibody was calculated as a number of positive nuclear reaction over 100 cells.Immunoreactivity for c-KIT and PDGFRα was evaluated semiquantitatively(weak,intermediate,strong) and for c-KIT type of reactivity was analyzed(cytoplasmic,membrane and 'dot-like' staining).Immunoreactivity for SMA,CD34 and S100 were was evaluated as positive or negative antigen expression.Pathologic parameters investigated in this study included tumor size,cell type(pure spindle,pured epitheloid mixed spindle and epitheloid),mitotic count,hemorrhage,necrosis,mucosal ulceration.Clinical data included age,gender,primary tumor location and spread of disease.χ 2 test and Student's t-test were used for comparisons of baseline characteristics.The Cox's proportional hazard model was used for univariable and multivariable analyses.Survival rates were calculated by Kaplan-Meier method and statistical significance was determined by the log-rank test.RESULTS:According to the stage of disease,there were 36 patients with localized disease,29 patients with initially localized disease but with its recurrence in the period of follow up,and finally,35 patients had metastatic disease from the very beginning of disease.Tumor originated most commonly in the stomach(41%),small intestine was the second most common location(36%).The mean size of primary tumors was 6.5 cm.The mean duration of follow-up was 60 mo.Multiple parameters were analyzed for their effect on overall survival,but no one reached statistical significance(P = 0.06).Analysis of time to progression/relapse in initially localized disease(univariate analysis),tumor size,mitotic count,Ki-67 and type of d-KIT distribution(cytoplasmic vs membrane/'dot-like') showed statistically significant correlation.In multivariate analysis in the group of patients with localized disease,there were only 2 parameters that have impact on relapse,Ki-67 and SMA(P < 0.0001 and P < 0.034,respectively).Furthermore,Ki-67 was analyzed in localized diseasevs localized with recurrence and metastatic disease.It was shown that there is a strict difference between these 2 groups of patients(median value was 2.5 for localized disease vs 10.0 for recurrent/metastatic disease,P < 0.0001).It was also shown that the cut-off value which is still statistically significant in terms of relapse on the level of 6%.The curves for survival on that cut-off level are significantly different(P < 0.04,Cox F).CONCLUSION:Ki-67 presents a significant prognostic factor for GIST recurrence which could be of great importance in evaluating malignant potential of disease. | Borislav Belev Iva Bri Juraj Prejac Zrna Antunac Golubi Damir Vrbanec Jadranka Bozikov Ivan Aleri Marko Boban Jasminka Jaki Razumovi | 2013 | World Journal of Gastroenterology2013，19，4： | 14 |
| 2 | Higher vitamin D serum concentration increases health related quality of life in patients with inflammatory bowel diseases显示文摘AIM: To investigate the effect of vitamin D(VD) concentrations and VD supplementation on health related quality of life in inflammatory bowel disease(IBD) patients. METHODS: A cohort of 220 IBD patients including 141 Crohn's disease(CD) and 79 ulcerative colitis(UC) patients was followed-up at a tertiary IBD center. A subgroup of the cohort(n = 26) took VD supplements for > 3 mo. Health related quality of life was assessed using the short IBD questionnaire(s IBDQ). VD serum concentration and s IBDQ score were assessed between August and October 2012(summer/autumn period) and between February and April 2013(winter/spring period). The mean VD serum concentration and its correlation with disease activity of CD were determined for each season separately. In a subgroup of patients, the effects of VD supplementation on winter VD serum concentration, change in VD serum concentration from summer to winter, and winter s IBDQ score were analyzed.RESULTS: During the summer/autumn and the winter/spring period, 28% and 42% of IBD patients were VD-deficient(< 20 ng/m L), respectively. In the winter/spring period, there was a significant correlation between s IBDQ score and VD serum concentration in UC patients(r = 0.35, P = 0.02), with a trend towards significance in CD patients(r = 0.17, P = 0.06). In the winter/spring period, VD-insufficient patients(< 30 ng/m L) had a significantly lower mean s IBDQ score than VD-sufficient patients; this was true of both UC(48.3 ± 2.3 vs 56.7 ± 3.4, P = 0.04) and CD(55.7 ± 1.25 vs 60.8 ± 2.14, P = 0.04) patients. In all analyzed scenarios(UC/CD, the summer/autumn period and the winter/spring period), health related quality of life was the highest in patients with VD serum concentrations of 50-59 ng/m L. Supplementation with a median of 800 IU/d VD day did not influence VD serum concentration or the s IBDQ score.CONCLUSION: VD serum concentration correlated with health related quality of life in UC and CD patients during the winter/spring period. | Tibor Hlavaty Anna Krajcovicova Tomas Koller Jozef Toth Monika Nevidanska Martin Huorka Juraj Payer | 2014 | World Journal of Gastroenterology2014，20，42： | 11 |
| 3 | 基于模型模拟的中国秸秆还田固碳潜力空间格局分析(英文)显示文摘农作物秸秆还田作为农田土壤和养分管理的推荐做法之一,对于土壤有机碳(SOC)固定和CO_2减排具有重要意义。本研究利用环境政策综合气候模型(EPIC)模拟了4种作物秸秆还田情形下2001年至2010年中国农田表层土壤有机碳变化及其空间格局。模拟结果显示,秸秆完全移除(CR0%)下的土壤有机碳损失为28.89 Tg yr^(–1),当前30%的秸秆还田(CR30%)能够减缓22.38 Tg C yr^(–1)的碳损失。若秸秆还田率从30%提高至50%(CR50%)乃至75%(CR75%),中国农田表层土壤将变为净碳汇。中国农田表层土壤固碳潜力在CR50%和CR75%情形下分别可达25.53 Tg C yr^(–1)和52.85 Tg C yr^(–1),且在不同农业区存在空间异质性。单位面积土壤固碳潜力在西北和华北地区最高,而华东最低。华北地区具有最高的区域固碳潜力。在这十年间,CR50%和CR75%情形下增加的土壤表层有机碳相当于减少了1.4%和2.9%的中国CO_2排放总量。总之,我们建议鼓励我国农民将原本直接焚烧或用作家用燃料的秸秆返还田间以改善土壤性质和减缓大气CO_2增加,尤其是华北地区更应推行这一举措。 | 陈敬华 王绍强 Florian KRAXNER Juraj BALKOVIC 徐希燕 孙雷刚 | 2019 | Journal of Resources and Ecology2019，10，2： | 5 |
| 4 | Drug-induced liver injury in inflammatory bowel disease: 1-year prospective observational study显示文摘AIM To analyze 1-year liver injury burden in inflammatory bowel disease(IBD) patients. METHODS During a 6-mo inclusion period, consecutive IBD cases having a control visit at IBD center were included. Basic demographics, IBD phenotype and IBD treatment were recorded on entry. Aminotransferase(AT) activities of ALT, AST, ALP and gamma-glutamyl transpeptidase(GGT) were measured at baseline, 3 mo prior to study entry and prospectively every 3 mo for 1 year. Liver injury patterns were predefined as: Grade 1 in ALT 1-3 × upper limit of normal(ULN), grade 2 in ALT > 3 × ULN, hepatocellular injury in ALT > 2 × ULN, cholestatic injury in simultaneous GGT and ALP elevation > ULN.Persisting injury was reported when AT elevations were found on > 1 measurement. Risk factors for the patterns of liver injury were identified among demographic parameters, disease phenotype and IBD treatment in univariate and multivariate analysis. Finally, implications for the change in IBD management were evaluated in cases with persisting hepatocellular or cholestatic injury.RESULTS Two hundred and fifty-one patients were included having 917 ALT and 895 ALP and GGT measurements. Over one year, grade 1 injury was found in 66(26.3%), grade 2 in 5(2%) and hepatocellular injury in 16 patients(6.4%). Persisting hepatocellular injury was found in 4 cases. Cholestasis appeared in 11 cases(4.4%) and persisted throughout the entire study period in 1 case. In multivariate analysis, hepatocellular injury was associated with BMI(OR = 1.13, 1.02-1.26), liver steatosis(OR = 10.61, 2.22-50.7), IBD duration(1.07, 1.00-1.15) and solo infliximab(OR = 4.57, 1.33-15.7). Cholestatic liver injury was associated with prior intestinal resection(OR = 32.7, 3.18-335), higher CRP(OR = 1.04, 1.00-1.08) and solo azathioprine(OR = 10.27, 1.46-72.3). In one case with transient hepatocellular injury azathioprine dose was decreased. In 4 cases with persisting hepatocellular injury, fatty liver or alcohol were most likely causes and IBD treatment was pursued without change. In the case with persisting cholestatic injury, no signs of portal hypertension were identified and treatment with infliximab continued.CONCLUSION Liver injury was frequent, mostly transient and rarely changed management. Infliximab or azathioprine were confirmed as its risk factors indicating the need for regular AT monitoring. | tomas koller martina galambosova simona filakovska michaela kubincova tibor hlavaty jozef toth anna krajcovicova juraj payer | 2017 | World Journal of Gastroenterology2017，23，22： | 5 |
| 5 | Mastitis Pathogens Isolated from Samples of Milk in Dairy Cows and Their Resistance against Antimicrobial Agents显示文摘The objective of this study was to evaluate the effectiveness of different antibiotics against mastitis causing microorganisms during first phase of lactation in herd of 320 Holstein cows in east of Slovakia. Milk samples from quarters were cultured and identified bacteria were subjected to antimicrobial susceptibility test by disc diffusion method to a large number of antibiotics. The results revealed higher sensitivity against tetradelta (100% of Strep. Agalactiae and Strep. uberis, Coagulase Negative Staphylococci), (94.4% of Stap. aureus) with highest number of bacterial isolates, followed by cefalexin + kanamycin amoxicillin plus clavulanat acid and ceftiotur (100% of Strep. agalactiae and Strep. uberis). Resistance was observed against penicillin (22.2% of Stap. aureus) amoxicillin (22.2% of Stap. aureus and 10.5% of Strep. uberis) and streptomycin (22.2% of Stap. aureus and 52.7% of Strep. uberis). | Frantiek Zigo Milan Vasil Juraj Elecko Martina Zigová Zuzana Farkasova | 2017 | Journal of Food Science and Engineering2017，7，2： | 4 |
| 6 | Role of omega-3 polyunsaturated fatty acids,citrus pectin,and milk-derived exosomes on intestinal barrier integrity and immunity in animals显示文摘The gastrointestinal tract of livestock and poultry is prone to challenge by feedborne antigens,pathogens,and other stress factors in the farm environment.Excessive physiological inflammation and oxidative stress that arises firstly disrupts the intestinal epithelial barrier followed by other components of the gastrointestinal tract.In the present review,the interrelationship between intestinal barrier inflammation and oxidative stress that contributes to the pathogenesis of inflammatory bowel disease was described.Further,the role of naturally existing immunomodulatory nutrients such as the omega-3 polyunsaturated fatty acids,citrus pectin,and milk-derived exosomes in preventing intestinal barrier inflammation was discussed.Based on the existing evidence,the possible molecular mechanism of these bioactive nutrients in the intestinal barrier was outlined for application in animal diets. | Tamil Selvi Sundaram Carlotta Giromini Raffaella Rebucci Juraj Pistl Mangesh Bhide Antonella Baldi | 2022 | Journal of Animal Science and Biotechnology2022，13，5： | 3 |
| 7 | Mortality of therapeutic fish Garra rufa caused by Aeromonas sobria显示文摘Objective:To investigate a case of mass mortality of Garra rufa(G.rufa)from a fish hatchery farm in Slovakia.Methods:Causative bacterial agent was swabbing out of affected fish skin area and subsequently identified using commercial test system.Antibiotic susceptibility was determined by the disk diffusion method.Results:Infected G.rufa was characterized by abnormal swimming behaviour,bleeding of skin lesions and local haemorrhages.Despite of using recommended aquatic antibiotic treatment no improvement was achieved and Aeromonas sobria{A.sobria)was identified as a causative agent of fish mortality.Due to massive fish mortality,antibiotic susceptibility of pure isolated culture of A.sobria was evaluated employing eight antibiotics against human infections.A.sobria was resistant only against one antibiotic,namely ampicilin.Conclusions:These results indicate that A.sobria can act as a primary pathogen of G.rufa and may be a potential risk factor for immunodeficient or immunoincompetent patients during the ichthyotherapy. | Juraj Majtán Jaroslav erny Alena Ofúkaná Peter Taká Milan Kozánek | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，2： | 3 |
| 8 | Endoscopic ultrasound elastography strain histograms in the evaluation of patients with pancreatic masses显示文摘AIM: To investigate the accuracy of the strain histogram endoscopic ultrasound(EUS)-based method for the diagnostic differentiation of patients with pancreatic masses. METHODS: In a prospective single center study, 149 patients were analyzed, 105 with pancreatic masses and 44 controls. Elastography images were recorded using commercially available ultrasound equipment in combination with EUS linear probes. Strain histograms(SHs) were calculated by machine integrated software in regions of interest and mean values of the strain histograms were expressed as Mode 1(over the mass) and Mode 2(over an adjacent part of pancreatic tissue, representing the reference area). The ratio between Mode 2 and Mode 1 was calculated later, representing a new variable, the strain histogram ratio. After the final diagnosis was established, two groups of patients were formed: a pancreatic cancer group with positive cytology achieved by fine needle aspiration puncture or histology after surgery(58 patients), and a massforming pancreatitis group with negative cytology and follow-up after 3 and 6 mo(47 patients). All statistical analyses were conducted in SPSS 14.0(SPSS Inc., Chicago, IL, United States).RESULTS: Results were obtained with software for strain histograms with reversed hue scale(0 represents the hardest tissue structure and 255 the softest). Based on the receiver operating characteristics(ROC) curve coordinates, the cut-off point for Mode 1 was set at the value of 86. Values under the cut-off point indicated the presence of pancreatic malignancy. Mode 1 reached 100% sensitivity and 45% specificity with overall accuracy of 66%(95%CI: 61%-66%) in detection of pancreatic malignant tumors among the patients with pancreatic masses. The positive and negative predictive values were 54% and 100%, respectively. The cut-off for the new calculated variable, the SH ratio, was set at the value 1.153 based on the ROC curve coordinates. Values equal or above the cut-off value were indicative of pancreatic malignancy. The SH ratio reached 98% sensitivity, 50% specificity and an overall accuracy of 69%(95%CI: 63%-70%). The positive and negative predictive values were 92% and 100%, respectively.CONCLUSION: SH showed high sensitivity in pancreatic malignant tumor detection but disappointingly low specificity. Slight improvements in specificity and accuracy were achieved using the SH ratio. | Dalibor Opa?i? Nadan Rustemovi? Mirjana Kalauz Pave Marko? Zvonimir Ostoji? Matea Majerovi? Iva Ledinsky Ana Vi?nji? Juraj Krznari? Milorad Opa?i? | 2015 | World Journal of Gastroenterology2015，21，13： | 3 |
| 9 | Large-scale HCV RNA screening in first time blood donors: the first step towards genomic screening of blood donations显示文摘 | Juraj P Patricia H John B | 1999 | Vox Sang1999，76，3： | 1 |
| 10 | Thermoanaytical study of methyl methacrylate polymerization under thermal gradient 显示文摘 | Juraj Doupovee Frantigek Suehy Emilia llekovu | 1996 | Thermochimica Acta1996，，： | 1 |
| 11 | Multivariate Interpolation of Precipitation Using Regularized Spline with Tension显示文摘 | JARSOLAV HOFIERKA JURAJ PARAJKA HELENA MITASOVA | 2002 | Transactions in GIS2002，6，2： | 1 |
| 12 | Reconstruction of the anterior cruciate ligament: dynamic strain evaluation of the graft显示文摘 | Milan Handl Milan Dr?ík Giuliano Cerulli Ctibor Povy?il Juraj Chlpík Ferdinand Varga Ev?en Amler Tomá? Tr? | 2007 | Knee Surgery Sports Traumatology Arthroscopy2007，，3： | 1 |
| 13 | Role of orthoptics and scoring system for orbital floor blowout fracture:surgical or conservative treatment显示文摘AIM:To assess the role of orthoptics in referring patients with orbital floor blowout fracture(OFBF)for conservative or surgical treatment and based on the results,to propose a scoring system for such decision making.METHODS:A retrospective analysis of 69 patients with OFBF was performed(35 treated conservatively,34 surgically).The role of orthoptics in referring to surgery or conservative treatment was retrospectively evaluated,the factors with the highest significance for decision making were identified,and a scoring system proposed using Logistic regression.RESULTS:According to defined criteria,the treatment was unsuccessful in 2(6%)surgically treated and only in one(3%)conservatively treated patient.The proposed scoring system includes the defect size and several values resulting from the orthoptic examination,the elevation of the eyebulb measured on Lancaster screen being the most significant.CONCLUSION:The study demonstrates the benefits of orthoptic examination when making decisions on conservative or surgical treatment and for diagnosing ocular motility disorder(with or without binocular diplopia)in OFBF patients.The proposed scoring system could,following verification in a prospective study,become a valuable adjunctive tool. | Juraj Timkovic Jiri Stransky Katerina Janurova Petr Handlos Jan Stembirek | 2021 | International Journal of Ophthalmology(English edition)2021，14，12： | 1 |
| 14 | Heat and mass transfer in the evaporating film of a molecular evaporator显示文摘 | JURAJ LUTISAN A | 2002 | Chemical Engineering Journal2002，，85： | 1 |
| 15 | Blast furnace slaga as sorbents of phosphate from water solutions显示文摘 | Bruno Kostura Hana Kulveitova Juraj Lesko | 2005 | Water Research2005，39，： | 1 |
| 16 | High organic carbon stock in a karstic soil of the Middle-European Forest Province persists after centuries-long agroforestry management显示文摘 | Yousif Abdel-Rahman Ahmed Viliam Pichler Marián Homolák Erika G?m?ryová Dezs? Nagy Magdaléna Pichlerová Juraj Gregor | 2012 | European Journal of Forest Research2012，，6： | 1 |
| 17 | Renal Doppler ultrasonography in infants with hydronephrosis 显示文摘 | Juraj R Svitae N Mirko Z | 2001 | International Urology and Nephrology2001，33，4： | 1 |
| 18 | Balance equations for molecular distillation显示文摘 | Miroslav M Juraj L Jan C | 1997 | Separation Science and Technology1997，32，18： | 1 |
| 19 | Blast furnace slags as sorbents of phosphate from water solutions显示文摘 | Kostura B Kulveitova H Juraj L | 2005 | Water Research2005，39，9： | 1 |
| 20 | Railway wheel and rail roughness analy- sis 显示文摘 | Tomas L Juraj G | 2009 | Komunikacie2009，11，2： | 1 |
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