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| 1 | Application of serine integrases for secondary metabolite pathway assembly in Streptomyces显示文摘Serine integrases have been shown to be efficient tools for metabolic pathway assembly.To further improve the flexibility and efficiency of pathway engineering via serine integrases,we explored how multiple orthogonally active serine integrases can be applied for use in vitro for the heterologous expression of complex biosynthesis pathways in Streptomyces spp.,the major producers of useful bioactive natural products.The results show that multiple orthogonal serine integrases efficiently assemble the genes from a complex biosynthesis pathway in a single in vitro recombination reaction,potentially permitting a versatile combinatorial assembly approach.Furthermore,the assembly strategy also permitted the incorporation of a well-characterised promoter upstream of each gene for expression in a heterologous host.The results demonstrate how site-specific recombination based on orthogonal serine integrases can be applied in Streptomyces spp. | Hong Gao Gabrielle Taylor Stephanie K.Evans Paul C.M.Fogg Margaret C.M.Smith | 2020 | Synthetic and Systems Biotechnology2020，5，2： | 3 |
| 2 | 古希腊罗马的妓女及其价值观显示文摘古代希腊罗马世界的经济发展模式是现代资本主义的、还是自然经济的、抑或是'嵌入型'的模式,一直存在争议。这显示了古典时期经济发展的复杂性。但这些模式存在明显的纰漏:只整合了城市男性精英群体的价值观并得到了我们一直使用的文献资料的强化。一个修正和平衡的模式无疑是广大下层民众的经济活动及其价值观的揭示。古代希腊罗马妓女的世界恰好是这一修正模式的最好载体。 | John K.Evans 沈扬(译校) 黄洋(译校) | 2007 | 历史研究2007，，3： | 1 |
| 3 | Addition of the neurokinin 1 receptor antagonist aprepitant to standard antiemetic therapy improves control of chemotherapy‐induced nausea and vomiting显示文摘 | SergioPoli‐Bigelli JoseRodrigues‐Pereira Alexandra D.Carides GuoguangJulie Ma KristaEldridge AnitaHipple Judith K.Evans Kevin J.Horgan FrancescaLawson | 2003 | Cancer2003，，12： | 1 |
| 4 | Soft tissue coverage of lower extremity defects:pearls and pitfalls in the chronic wound population显示文摘The incidence of chronic lower extremity(LE)wounds continues to increase.Lower limb amputations are associated with increased cardiovascular exertion,further decline in functional ability,and higher mortality rates.As such,there has been a shift towards limb salvage modalities.These include local debridement with advanced wound care,revascularization,bony reconstruction,and soft tissue reconstruction.Perioperative planning for soft tissue reconstruction requires careful consideration of several factors,including patient comorbidities,wound size and location,exposed underlying structures,and in the case of possible free flap,patency of donor and recipient vessels.This article reviews the perioperative factors that should be considered in preparation for successful soft tissue reconstruction of the LE. | Romina Deldar Chamilka Merle Christopher E.Attinger Karen K.Evans | 2022 | Plastic and Aesthetic Research2022，9，1： | 0 |
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