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| 1 | Fibroblasts Accelerate Formation and Improve Reproducibility of 3D Cellular Structures Printed with Magnetic Assistance显示文摘Fibroblasts(mouse,NIH/3T3)are combined with MDA-MB-231 cells to accelerate the formation and improve the reproducibility of 3D cellular structures printed with magnetic assistance.Fibroblasts and MDA-MB-231 cells are cocultured to produce 12.5:87.5,25:75,and 50:50 total population mixtures.These mixtures are suspended in a cell medium containing a paramagnetic salt,Gd-DTPA,which increases the magnetic susceptibility of the medium with respect to the cells.A 3D monotypic MDA-MB-231 cellular structure is printed within 24 hours with magnetic assistance,whereas it takes 48 hours to form a similar structure through gravitational settling alone.The maximum projected areas and circularities,and cellular ATP levels of the printed structures are measured for 336 hours.Increasing the relative amounts of the fibroblasts mixed with the MDA-MB-231 cells decreases the time taken to form the structures and improves their reproducibility.Structures produced through gravitational settling have larger maximum projected areas and cellular ATP,but are deemed less reproducible.The distribution of individual cell lines in the cocultured 3D cellular structures shows that printing with magnetic assistance yields 3D cellular structures that resemble in vivo tumors more closely than those formed through gravitational settling.The results validate our hypothesis that(1)fibroblasts act as a“glue”that supports the formation of 3D cellular structures,and(2)the structures are produced more rapidly and with greater reproducibility with magnetically assisted printing than through gravitational settling alone.Printing of 3D cellular structures with magnetic assistance has applications relevant to drug discovery,lab-on-chip devices,and tissue engineering. | Sarah Mishriki Srivatsa Aithal Tamaghna Gupta Rakesh P.Sahu Fei Geng Ishwar K.Puri | 2020 | Research2020，，1： | 1 |
| 2 | Cutaneous metastatic cholangiocarcinoma: a report of three cases and review of the literature显示文摘 | Kelly L.West Maria AngelicaSelim Puja K.Puri | 2010 | Journal of Cutaneous Pathology2010，，12： | 1 |
| 3 | Fragment emission and critical behavior in light and heavy charged systems显示文摘We study the emission of fragments in central collisions of light and heavily charged systems of^4+Ar+^45 Sc and^s4Kr+^197 Au,respectively,using the Quantum Molecular Dynamics(QMD)model as the primary model.The fragments are identified using an energy based clusterization algorithm,i.e.,the Simulated Annealing Clusterization Algorithm(SACA).The charge distributions of intermediate mass fragments[3≤Zf≤12]are fitted with power-law(∝Zf^-τ)and exp on ential(∝e^-λZf)fits in order to extract the parametersτandλ,whose minimum values are also sometimes linked with the onset of fragmentation or the critical point for a liquid-gas phase transition.Other parameters such as the normalized second moment,<γ2>,average size of the second largest cluster,phase separation parameter(Sp),bimodal parameter(P),information entropy(H),and Zipfs law are also analyzed to find the exact energy of the onset of fragmentation.Our detailed analysis predicts that an energy point exists between 20-23.1 MeV/nucleon,which is very close to the experimentally observed value of 23.9 MeV/nucleon for the^40Ar+^45Sc reaction.We also find that the critical energy deduced using Zipfs law is higher than those predicted from other critical exponents.Moreover,no minimum is found for t values of the highly charged system of^84Kr+^197Au,in agreement with experimental findings and various theoretical calculations.We observe that the QMD+SACA model calculations are in agreement with the experimental observations.This agreement supports our results regarding the energy point of the liquid-gas phase transition and the onset of fragmentation. | Samiksha Sood Rohit Kumar Arun Sharma Sakshi Gautam Rajeev K.Puri | 2021 | Chinese Physics C2021，45，1： | 0 |
| 4 | Potential therapeutic interventions based on the role of the endoplasmic reticulum stress response in progressive neurodegenerative diseases显示文摘In 1945,Porter et al.published an electon microscopy study of cultured chick fibroblasts in which they observed:‘a granular background and details of a darker lacelike reticulum which in places appears to be made up of chains of'vesicles'’(Porter et al.,1945).This constituted the first published observation of the endoplasmic reticulum(ER)and,while it was not evident at that time。 | Basant K.Puri Gerwyn Morris | 2018 | Neural Regeneration Research2018，13，11： | 0 |
| 5 | Estimates for Lyme borreliosis infections based on models using sentinel canine and human seroprevalence data显示文摘Two models were developed to estimate Lyme borreliosis(LB)cases.One was based on the seroprevalence of Borrelia infections in human samples.This model used corrections for false negative and false positive results from published test sensitivity and specificity measures.A second model based on Borrelia infections in sentinel dogs was used to quantify the prevalence of Lyme disease Borrelia infections in humans;the reference baseline for this model was human and canine infections in Germany.A comparison of the two models is shown and differences discussed.The relationships between incidence,prevalence and total infection burden for LB were derived from published data and these were used in both models to calculate annual incidence,prevalence and total LB infections.The modelling was conservative and based on medical insurance records coded for erythema migrans.Linear model growth rates were used in place of the commonly adopted exponential growth.The mean of the two models was used to create estimates for various countries and continents.Examples from the analyses for LB estimated for 2018 include:incidence e USA 473,000/year,Germany 471,000/year,France 434,000/year and UK 132,000/year;prevalence e USA 2.4 million,Germany 2.4 million,France 2.2 million and UK 667,000;total infections e USA 10.1 million,Germany 10.0 million,France 9.3 million and UK 2.8 million.Estimates for the world for 2018 are:incidence 12.3 million/year;prevalence 62.1 million;and total infection burden 262.0 million.These figures are far higher than officially published data and reflect not only the underestimation of diagnosed cases,which is acknowledged by health agencies,but also undiagnosed and misdiagnosed cases. | Michael J.Cook Basant K.Puri | 2020 | Infectious Disease Modelling2020，5，1： | 0 |
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