| 1 | 连接蛋白基因表达与横纹肌肉瘤分化关系研究显示文摘报道了用间隙连接蛋白Cx43基因探针斜缝杂交,Cx43和肌专一蛋白免疫荧光染色和罗氏黄荧光传输法观察到人横纹肌肉瘤(RD细胞系)的分化异常,细胞通讯缺陷,Cx43表达下降。单细胞克隆亚系RDL6增殖活跃,分化差,细胞通讯缺陷,Cx43表达很低;另一亚系RDL3增殖慢,有明显肌分化,通讯功能强,Cx43及其mRNA的表达近似正常成肌细胞。Cx43 cDNA+neo基因转染RDL6得到Cx43稳定表达的克隆RDL6/C-4,其通讯功能恢复,增殖抑制,成肌性分化增强。本结果证明RD细胞分化阻断与Cx43降表达有关。Cx43的功能性表达不仅抑制瘤细胞增殖,而且与瘤细胞向正常分化有正相关性。 | 林仲翔 张志谦 韩亚玲 C.C.G.Naus K.R.Yu H.Holtzer | 1994 | 中国科学(B辑)1994，24，6： | 8 |
| 2 | Functional expression of gap junction gene Cx43 and the myogenic differentiation of rhabdomyosarcoma cells显示文摘Rhabdomyosarcoma (RD) cells express low levels of the gap junction protein connexin 43 (Cx43), and its mRNA, and display very weak gap junctional intercellular communication (GJIC) as detected by Cx43 immunofluorescence, slot-blot and dye-transfer methods. These cells grow rapidly and show aberrant and incomplete myogenic differentiation. To investigate the role of gap junctions in these cells, the expression of Cx43 with relation to cell growth and myogenic differentiation in RD single-cell subclones-RDL3 and RDL6 is studied. The subclone RDL3 grows slowly and displays better myogenic differentiation. The expression of Cx43, its mRNA and the GJIC in RDL3 is comparable to that in normal myoblasts. Another subclone RDL6 which grows rapidly, but is poorly differentiated, expresses very low levels of Cx43 and its mRNA, and very weak GJIC. By using the calcium phosphate precipitate transfection technique, a full-length cDNA-encoding Cx43 and a pSV2neo have been introduced into the RDL6 cells. Several | 林仲翔 张志谦 韩亚玲 C.C.G.Naus K.R.Yu H.Holtzer | 1995 | Science China Chemistry1995，38，3： | 0 |
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