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| 1 | Effect of Helicobacter pylori cdrA on interleukin-8 secretions and nuclear factor kappa B activation显示文摘AIM:To investigate genetic diversity of Helicobacter pylori (H.pylori) cell division-related gene A (cdrA) and its effect on the host response.METHODS:Inactivation of H.pylori cdrA,which is involved in cell division and morphological elongation,has a role in chronic persistent infections.Genetic property of H.pylori cdrA was evaluated using polymerase chain reaction and sequencing in 128 (77 American and 51 Japanese) clinical isolates obtained from 48 and 51 patients,respectively.Enzyme-linked immunosorbent assay was performed to measure interleukin-8 (IL-8) secretion with gastric biopsy specimens obtained from American patients colonized with cdrA-positive or-negative strains and AGS cells cocultured with wild-type HPK5 (cdrA-positive) or its derivative HPKT510 (cdrA-disruptant).Furthermore,the cytotoxin-associated gene A (cagA) status (translocation and phosphorylation) and kinetics of transcription factors [nuclear factor-kappa B (NF-κB) and inhibition kappa B] were investigated in AGS cells co-cultured with HPK5,HPKT510 and its derivative HPK5CA (cagA disruptant) by western blotting analysis with immunoprecipitation.RESULTS:Genetic diversity of the H.pylori cdrA gene demonstrated that the cdrA status segregated into two categories including four allele types,cdrA-positive (allele types;Ⅰand Ⅱ) and cdrA-negative (allele types;Ⅲ and Ⅳ) categories,respectively.Almost all Japanese isolates were cdrA-positive (Ⅰ:7.8% and Ⅱ:90.2%),whereas 16.9% of American isolates were cdrA-positive (Ⅱ) and 83.1% were cdrA-negative (Ⅲ:37.7% and Ⅳ:45.5%),indicating extended diversity of cdrA in individual American isolates.Comparison of each isolate from different regions (antrum and corpus) in the stomach of 29 Americans revealed that cdrA status was identical in both isolates from different regions in 17 cases.However,12 cases had a different cdrA allele and 6 of them exhibited a different cdrA category between two regions in the stomach.Furthermore,in 5 of the 6 cases possessing a different cdrA category,cdrA-negative isolate existed in the corpus,suggesting that cdrA-negative strain is more adaptable to colonization in the corpus.IL-8 secretions from AGS revealed that IL-8 levels induced by a cdrA-disrupted HPKT510 was significantly lower (P < 0.01) compared to wildtype HPK5:corresponding to 50%-60% of those of wild-type HPK5.These data coincided with in vivo data that an average value of IL-8 in biopsy specimens from cdrA-positive and cdrA-negative groups was 215.6 and 135.9 pg/mL,respectively.Western blotting analysis documented that HPKT510 had no effect on CagA translocation and phosphorylation,however,nuclear accumulation of NF-κB was lower by HPKT510 compared to HPK5.CONCLUSION:Colonization by a cdrA-negative or cdrA-dysfunctional strain resulted in decreased IL-8 production and repression of NF-κB,and hence,attenuate the host immunity leading to persistent infection. | Hiroaki Takeuchi Ya-Nan Zhang Dawn A Israel Richard M Peek Jr Mikio Kamioka Hideo Yanai Norihito Morimoto Tetsuro Sugiura | 2012 | World Journal of Gastroenterology2012，18，5： | 3 |
| 2 | Speciation of sulfate in size-fractionated aerosol particles using sulfur K-edge X-ray Absorption Near-Edge Structure 显示文摘 | Takahashi Y Kanai Y Kamioka H | 2006 | Environmental Science and Technology2006，40，： | 1 |
| 3 | Insertion/deletion coding polymorphisms in hHAVcr-1 are not associated with atop ic asthma in the Japanese population显示文摘 | Noguchi E Nakayama J Kamioka M | 2003 | Genes&Immunity2003，4，2： | 1 |
| 4 | 显示文摘 | Kamioka H Hiramatsu H | 2004 | Journal of Photochemistry and Photobiology A: Chemistry2004，166，： | 1 |
| 5 | A Novel Dynamin-associating Molecule,Formin-binding Protein 17,Induces Tubular Membrane Invaginations and Participates in Endocytosis显示文摘 | KAMIOKA Y FUKUHARA S SAWA H | 2004 | J Biol Chem2004，279，40： | 1 |
| 6 | Asian continental nature of ^87Sr/^86Sr ratios in north central Pacific sediments显示文摘 | Asahara Y Tanaka T Kamioka H | 1995 | Earth and Planetary Science Letters1995，133，： | 1 |
| 7 | Provenance of the north Pacific sediments and process of source material transport as derived from Rb-Sr isotopic systematics显示文摘 | Asahara Y Tanaka T Kamioka H | 1999 | Geochemical Geology1999，158，： | 1 |
| 8 | Hormonal, pH, and calcium regulation of eonnexin 43 - mediated dye transfer in osteoeytes in chick ealvaria显示文摘 | Ishihara Y Kamioka H Honjo T | 2008 | J Bone Miner Res2008，23，： | 1 |
| 9 | Blockade of reninangiotensin system attenuates advanced glycation end productsmediated signaling pathways显示文摘 | Kamioka M Ishibashi T Sugimoto K | | 0，，06： | 1 |
| 10 | JNdi-l: A neodymium isotopic reference in consistency with Lalolla neodymium 显示文摘 | Tanaka T Togashi S Kamioka H Amakawa H Kagami H Hamamoto T Yuhara M Orihashi Y J Yoneda S Shimizu H Kunimaru T Takahashi K Yanagi T Nakano T Fujimaki H Shinjo R Asahara Y Tanimizu M Dragusanu C | 2000 | Chemical Geology2000，168，： | 1 |
| 11 | Bimaxillary pro- trusion with masseter muscle hypertrophy treated with titani-um screw anchorage and masseter surgical reduction 显示文摘 | Hashimoto T Kuroda S Kamioka H et aI | 2009 | Am J Orthod Dentofacial Orthop2009，135，4： | 1 |
| 12 | New polymorphisms of haematopoietic prostaglandin D synthase and human prostanoid DP receptor genes显示文摘 | Noguchi E Shibasaki M Kamioka M | | 0，，01： | 1 |
| 13 | A novel dynamin-associating molecule, formin-binding protein 17, induces tubular membrane invag- inations and partieipates in endoeytosis显示文摘 | Kamioka Y Fukuhara S Sawa H | 2004 | J Biol Chem2004，279，40： | 1 |
| 14 | Osteocyte shape is dependent on actin filaments and osteocyte processes are unique actin-rich projections显示文摘 | Tanaka-Kamioka K Kamioka H Ris H | 1998 | J Bone Miner Res1998，13，10： | 1 |
| 15 | Computer-aided differ- entiation of malignant from benign solitary pulmonary nodules imaged by high-resolution CT显示文摘 | Iwano S Nakamura T Kamioka Y | 2008 | Comput Med Imaging Graph2008，32，5： | 1 |
| 16 | Ras and Calcium Signaling Pathways Converge at Rafl viathe Shoc2 Scaffold Protein 显示文摘 | Matsunaga-Udagawa Kazuhiro Aoki Yuji Kamioka | 2010 | Mol Biol Cell2010，21，6： | 1 |
| 17 | Interaction of FOXO1 and TSC2 induces insulin resistance through activation of the mammalian target of rapamycin/p70 S6K pathway 显示文摘 | Cao Y Kamioka Y Yokoi N | 2006 | Biol Chem2006，281，40： | 1 |
| 18 | Metabolic effects of 20 kDa and 22 kDa human growth hormones on adult male spontaneous dwarf rats显示文摘 | Hiroi N Kamioka T | 2001 | Eur J Endocrinol2001，145，6： | 1 |
| 19 | Multiobjective reinforcement learning based on multiple value functions显示文摘 | Kamioka T Kamioka T Uchibe E | 2006 | IEIC Technical Report (Institute of Electronics Information and Communication Engineers)2006，105，658： | 1 |
| 20 | Insertion/deletion coding polymorphisms in hHAVcr-1 are not associated with atopic asthma in the Japanese population显示文摘 | Noguchi E Nakayama J Kamioka M | 2003 | Genes Immun2003，4，2： | 1 |
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