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| 1 | RNAi-Mediated Silencing of ITPK Gene Reduces Phytic Acid Content,Alters Transcripts of Phytic Acid Biosynthetic Genes,and Modulates Mineral Distribution in Rice Seeds显示文摘Phytic acid is the principal storage form of phosphorus in plant seeds and an essential signalling molecule in several regulatory processes of plant development.However,it is known as an anti-nutrient compound owing to its potent chelating property.Thus,reducing the phytic acid content in crops is desirable.Studies involving regulation of MIPS and IPK1 genes to generate low phytate rice have been reported earlier.However,the functional significance of OsITPK and the effect of its down-regulation on phytic acid content and the associated pleiotropic effects on rice have not yet been investigated.In this study,tissue specific RNA interference(RNAi)-mediated down-regulation of a major ITPK homolog(OsITP5/6K-1)resulted in 46.2%decrease in phytic acid content of T2 transgenic seeds with a subsequent 3-fold enhancement in the inorganic phosphorus content.Silencing of OsITP5/6K-1 altered the transcript levels of essential phytic acid pathway genes,without significantly affecting the transcript levels of other OsITPK homologs.Furthermore,the mapping of elements through X-ray microfluorescence analysis revealed significant changes in the spatial distribution pattern and translocation of elements in low phytate seeds.Additionally,low phytate polished seeds exhibited 1.3-fold and 1.6-fold enhancement in iron and zinc content in the grain endosperm,respectively.Silencing of OsITP5/6K-1 also altered the amino acid and myo-inositol content of the transgenic seeds.Our results successfully established that RNAi-mediated silencing of OsITP5/6K-1 gene significantly reduced the phytate levels in seeds without hampering the germination potential of seeds and plant growth.The present study provided an insight into the mechanism of phytic acid biosynthesis pathway. | Aritra KARMAKAR Sananda BHATTACHARYA Shinjini SENGUPTA Nusrat ALI Sailendra Nath SARKAR Karabi DATTA Swapan K.DATTA | 2020 | Rice science2020，27，4： | 7 |
| 2 | Evidence for genomic changes in transgenic rice (Oryza sativa L.) recovered from protoplasts显示文摘 | Phan Huy Bao Simona Granata Stefano Castiglione Gejiao Wang Chiara Giordani Elena Cuzzoni Giuseppe Damiani Claudio Bandi Swapan K. Datta Karabi Datta Ingo Potrykus Anna Callegarin Francesco Sala | 1996 | Transgenic Research1996，，2： | 1 |
| 3 | The Effect of Bath Temperature on the Crystallite Size and Microstructtn'e of Ni-CeO2 Nanocomposite Coating显示文摘 | Ranjan S Siddhartha D Karabi D | 2011 | Materials Characterization2011，62，3： | 1 |
| 4 | The effect of bath temperature on the crystallite size and microstrnc- ture of Ni - CeO2 nanocomposite coating 显示文摘 | RAN JAN S SIDDHARTHA D KARABI D | 2011 | Material Charactenzation2011，62，3： | 1 |
| 5 | Effect of stirring rate on the microstmcture and micmhardness of Ni -CeO2 nanocomposite coating and investigation of the corrosion property 显示文摘 | RANJAN S SIDDHARTHA D KARABI D | 2011 | Surface and Coating Technology2011，205，1314： | 1 |
| 6 | Economic evaluation of aclidinium bromide in the management of moderate to severe COPD:an analysis over 5 years显示文摘 | Karabis A Mocarski M Eijgelshoven I | 2014 | Clinicoecon Outcomes Res2014，6，2： | 1 |
| 7 | Field Performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis δ -endotoxin显示文摘 | Jumin Tu GuoanZhang Karabi Datta | 2000 | Nature Biotechnology2000，18，： | 1 |
| 8 | Agrobacterium mediated genetic transformation of commercial jute cultivar Corchorus capsularis cv. JRC 321 using shoot tip explants显示文摘 | Prosanta Saha Karabi Datta Shuvobrata Majumder Chirabrata Sarkar Shyamsundar P. China Sailendra N. Sarkar Debabrata Sarkar Swapan K. Datta | 2014 | Plant Cell, Tissue and Organ Culture (PCTOC)2014，，2： | 1 |
| 9 | RNAi mediated silencing of lipoxygenase gene to maintain rice grain quality and viability during storage显示文摘 | Dipak Gayen Nusrat Ali Moumita Ganguly Soumitra Paul Karabi Datta Swapan K. Datta | 2014 | Plant Cell, Tissue and Organ Culture (PCTOC)2014，，2： | 1 |
| 10 | Source Apportionment of the Atmospheric Aerosol in Lahore, Pakistan显示文摘 | Suresh Raja Karabi F. Biswas Liaquat Husain Philip K. Hopke | 2010 | Water, Air, and Soil Pollution2010，，1： | 1 |
| 11 | Annular elastolytic gi-ant cell granuloma of conjunctiva:A case report显示文摘 | Karabi Konar Saurabh Sanyal Arindam Rakshit | 2014 | Brief communications2014，62，3： | 1 |
| 12 | 显示文摘 | Karabi D Bandyopadhyay T K | 2004 | Materials Science and Engineering A2004，379，12： | 1 |
| 13 | In-situ Al-based bulk nanocomposites by solid-state aluminothermic reaction in Al–Ti–O system显示文摘 | J. John Samuel Dilip B.S.B. Reddy Siddhartha Das Karabi Das | 2008 | Journal of Alloys and Compounds2008，，1： | 1 |
| 14 | Influence of duty cycle on the microstructure and microhardness of pulse electrodeposited Ni-CeO2 nanocomposite coating显示文摘 | Ranjan S Siddhartha D Karabi D | 2012 | Materials Research Bulletin2012，47，2： | 1 |
| 15 | Evolution of as - cast and heat - treated microstructure of a commercial beating alloy 显示文摘 | Pritha Choudhury Karabi Das Siddhartha Das | 2005 | Materials Science and Engineering2005，，398： | 1 |
| 16 | Indica rice(Oryza sativa,BR29 and IR64)显示文摘 | KARABI D KUMAR D S | 2006 | Methods in Molecular Biology2006，343，343： | 1 |
| 17 | Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars显示文摘 | KARABI D TU J OLIVA N | 2001 | Plant Science2001，160，3： | 1 |
| 18 | Effect of grain refinement on wear properties of A1 and AI-7Si alloy显示文摘 | A K Prasada Rao Karabi Das B S Murty | 2004 | Wear2004，257，： | 1 |
| 19 | The finite-buffer M/G/1 queue with general bulk-service rule and single vacation 显示文摘 | GUPTA U C KARABI S | 2004 | Performance Evaluation2004，57，： | 1 |
| 20 | Bioengineered 'golden' indica rice cuhivars with-carotene metabolism in the endosperm with hygromycin and mannose selection systems显示文摘 | Karabi D Niranjan B Norman O | 2003 | Plant Biotechnology Journal2003，1，： | 1 |
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