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| 1 | Oxidative stress-induced mitochondrial dysfunction in a normal colon epithelial cell line显示文摘AIM To determine how a normal human colon cell line reacts to microbial challenge as a way to study oxidative stress-induced responses associated with inflammatory bowel disease.METHODS Normal human colon epithelial cells(ATCC?CRL.1790?)were stimulated with either heat killed E.coli or heat killed murine cecal contents(HKC)and examined for several relevant biomarkers associated with inflammation and oxidative stress including cytokine production,mitochondrial autophagy and oxidant status.TNFα,IL-1βand IL-8 protein concentrations were measured within the supernatants.Fluorescent microscopy was performed to quantify the production of reactive oxygen species(ROS)using an oxidation responsive fluorogenic probe.Mitochondrial morphology and mitochondrial membrane potential was assessed by dual staining using COXIV antibody and a dye concentrating in active mitochondria.Mitochondrial ROS scavenger was used to determine the source of ROS in stimulated cells.Autophagy was detected by staining for the presence of autophagic vesicles.Positive controls for autophagy and ROS/RNS experiments were treated with rapamycin and chloroquine.Mitochondrial morphology,ROS production and autophagy microscopy experiments were analyzed using a custom acquisition and analysis microscopy software(Image J).RESULTS Exposing CRL.1790 cells to microbial challenge stimulated cells to produce several relevant biomarkers associated with inflammation and oxidative stress.Heat killed cecal contents treatment induced a 10-12fold increase in IL-8 production by CRL.1790 cells compared to unstimulated controls at 6 and 12 h(P<0.001).Heat killed E.coli stimulation resulted in a4-5 fold increase in IL-8 compared to the unstimulated control cells at each time point(P<0.001).Both heat killed E.coli and HKC stimulated robust ROS production at 6(P<0.001),and 12 h(P<0.01).Mitochondrial morphologic abnormalities were detected at 6 and12 h based on reduced mitochondrial circularity and decreased mitochondrial membrane potential,P<0.01.Microbial stimulation also induced significant autophagy at 6 and 12 h,P<0.01.Lastly,blocking mitochondrial ROS generation using mitochondrial specific ROS scavenger reversed microbial challenge induced mitochondrial morphologic abnormalities and autophagy.CONCLUSION The findings from this study suggest that CRL.1790cells may be a useful alternative to other colon cancer cell lines in studying the mechanisms of oxidative stress events associated with intestinal inflammatory disorders. | Nandakumar Packiriswamy Kari F Coulson Susan J Holcombe Lorraine M Sordillo | 2017 | World Journal of Gastroenterology2017，23，19： | 3 |
| 2 | Increased Risk of Hepatobiliary Cancers After Hospitalization for Autoimmune Disease显示文摘 | Felipe A. Castro Xiangdong Liu Asta F?rsti Jianguang Ji Jan Sundquist Kristina Sundquist Jill Koshiol Kari Hemminki | 2014 | Clinical Gastroenterology and Hepatology2014，，： | 1 |
| 3 | Measuring the symptom experience of Chinese cancer patients:a validation of the Chinese version of the memorial symptom assessment scale显示文摘 | KARIS K F CHENG R N | 2009 | J Pain Symptom Manage2009，37，1： | 1 |
| 4 | The evolution of cellular computing:nature's solution to a computational problem显示文摘 | Landweber L F Kari L | 1999 | Biosystems1999，52，13： | 1 |
| 5 | In vivo radioproteetion by the fullerene nanopartiele DF -1 as assessed in a zebrafish model 显示文摘 | Daroczi B Kari G McAleer M F Wolf J C Rodeck U Dieker A P | 2006 | Clin Cancer Res2006，12，： | 1 |
| 6 | Orotic aciduria caused by feeding excess lysine to growing rats 显示文摘 | Ulman E A Kari F W Hevia P | 1981 | J Nutr1981，111，： | 1 |
| 7 | Effects of pain, atigue, insomnia, and mood disturbance on functional status and quality of life of elderly patients with cancer显示文摘 | Karis K F Diana T F | 2011 | Critical Rev in Oncology/Hematology2011，78，2： | 1 |
| 8 | Long-term renal function in liver transplant recipients and impact of immunosuppressive regimens(calcineurin inhibitors alone or in combination with mycophenolate mofetil):the TRY study显示文摘 | Karie GS Janus N Saliba F | 2009 | LiverTranspl2009，15，9： | 1 |
| 9 | Genome-wide investigation of gene–environment interactions in colorectal cancer显示文摘 | Sabine Siegert Jochen Hampe Clemens Schafmayer Witigo Sch?nfels Jan-Hendrik Egberts Asta F?rsti Bowang Chen Jesús Lascorz Kari Hemminki Andre Franke Michael Nothnagel Ute N?thlings Michael Krawczak | 2013 | Human Genetics2013，，2： | 1 |
| 10 | Polymorphisms in the KDR and POSTN Genes: Association with Breast Cancer Susceptibility and Prognosis显示文摘 | Asta F?rsti Qianren Jin Andrea Altieri Robert Johansson Kerstin Wagner Kerstin Enquist Ewa Grzybowska Jolanta Pamula Wioletta Pekala G?ran Hallmans Per Lenner Kari Hemminki | 2007 | Breast Cancer Research and Treatment2007，，： | 1 |
| 11 | Dietary restriction reduces insulin-like growth factor I levels,which modulates apoptosis,cell proliferation,and tumor progression in p53-deficient mice显示文摘 | Dunn S E Kari F W French J | 1997 | Cancer Res1997，57，21： | 1 |
| 12 | Speciation and fate of ethylenediametetaacetate (EDTA) in municipal wastewater treatment 显示文摘 | Kari F G Giger W | 1996 | Water Research1996，30，1： | 1 |
| 13 | Polymorphisms in the KDR and POSTN Genes: Association with Breast Cancer Susceptibility and Prognosis显示文摘 | Asta F?rsti Qianren Jin Andrea Altieri Robert Johansson Kerstin Wagner Kerstin Enquist Ewa Grzybowska Jolanta Pamula Wioletta Pekala G?ran Hallmans Per Lenner Kari Hemminki | 2007 | Breast Cancer Research and Treatment2007，，1： | 1 |
| 14 | Growing season of Nordic mountain birch in nnrthemost Europe as indicated by long- term field studies and analyses of satellite inmges显示文摘 | sSHUTOVA E WIELGOIASKI F E KARIES S R | 2006 | International Jour nat of Biometeorology2006，51，： | 1 |
| 15 | Determination of dissolved and adsorbed EDTA species in water and sediments by HPLC显示文摘 | Nowack B Kari F G Hilger S U | 1996 | Analytical Chemistry1996，68，： | 1 |
| 16 | Determination of the reaction quantum yield for the photochemical degradation of Fe(Ш)-EDTA: implication for the environmental fate of EDTA in surface waters显示文摘 | Kari F G Hilger S Canonica S | 1995 | Environmental Science & Technology1995，29，： | 1 |
| 17 | The Evolution of Cellular Computing:Nature's Solution to a Computational Problem显示文摘 | Landweber L F Kari L | 1999 | Biosystems1999，52，1： | 1 |
| 18 | PAI-1 ?675 4G/5G polymorphism as a prognostic biomarker in breast cancer显示文摘 | Haixin Lei Kari Hemminki Robert Johansson Andrea Altieri Kerstin Enquist Roger Henriksson Per Lenner Asta F?rsti | 2008 | Breast Cancer Research and Treatment2008，，1： | 1 |
| 19 | Subsequent leukaemia in autoimmune disease patients显示文摘 | Kari Hemminki Xiangdong Liu Asta F?rsti Jianguang Ji Jan Sundquist Kristina Sundquist | 2013 | Br J Haematol2013，，5： | 1 |
| 20 | Polymorphisms in the Estrogen Receptor Beta Gene and Risk of Breast Cancer: No Association显示文摘 | Asta F?rsti Chunyan Zhao Elisabeth Israelsson Karin Dahlman-Wright Jan-?ke Gustafsson Kari Hemminki | 2003 | Breast Cancer Research and Treatment2003，，3： | 1 |
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