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| 1 | Lack of CD44 variant 6 expression in rectal cancer invasive front associates with early recurrence显示文摘AIM: To investigate the prognostic value of CD44 variant 6 (CD44v6), a membranous adhesion molecule, in rectal cancer. METHODS: Altogether, 210 rectal cancer samples from 214 patients treated with short-course radiotherapy (RT, n = 90), long-course (chemo) RT (n = 53) or surgery alone (n = 71) were studied with immunohistochemistry for CD44v6. The extent and intensity of membranous and cytoplasmic CD44v6 staining, and the intratumoral membranous staining pattern, were analyzed.RESULTS: Membranous CD44v6 expression was seen in 84% and cytoplasmic expression in 81% of the cases. In 59% of the tumors with membranous CD44v6 expression, the staining pattern in the invasive front was determined as 'front-positive' and in 41% as 'front-negative'. The latter pattern was associated with narrower circumferential margin (P = 0.01), infiltrative growth pattern (P < 0.001), and shorter disease-free survival in univariate survival analysis (P = 0.022) when compared to the 'front-positive' tumors. CONCLUSION: The lack of membranous CD44v6 in the rectal cancer invasive front could be used as a method to identify patients at increased risk for recurrent disease. | Suvi Tuulia Avoranta Eija Annika Korkeila Kari Juhani Syrjnen Seppo Olavi Pyrhnen Jari Toivo Tapio Sundstrm | 2012 | World Journal of Gastroenterology2012，18，33： | 9 |
| 2 | Oxidative stress-induced mitochondrial dysfunction in a normal colon epithelial cell line显示文摘AIM To determine how a normal human colon cell line reacts to microbial challenge as a way to study oxidative stress-induced responses associated with inflammatory bowel disease.METHODS Normal human colon epithelial cells(ATCC?CRL.1790?)were stimulated with either heat killed E.coli or heat killed murine cecal contents(HKC)and examined for several relevant biomarkers associated with inflammation and oxidative stress including cytokine production,mitochondrial autophagy and oxidant status.TNFα,IL-1βand IL-8 protein concentrations were measured within the supernatants.Fluorescent microscopy was performed to quantify the production of reactive oxygen species(ROS)using an oxidation responsive fluorogenic probe.Mitochondrial morphology and mitochondrial membrane potential was assessed by dual staining using COXIV antibody and a dye concentrating in active mitochondria.Mitochondrial ROS scavenger was used to determine the source of ROS in stimulated cells.Autophagy was detected by staining for the presence of autophagic vesicles.Positive controls for autophagy and ROS/RNS experiments were treated with rapamycin and chloroquine.Mitochondrial morphology,ROS production and autophagy microscopy experiments were analyzed using a custom acquisition and analysis microscopy software(Image J).RESULTS Exposing CRL.1790 cells to microbial challenge stimulated cells to produce several relevant biomarkers associated with inflammation and oxidative stress.Heat killed cecal contents treatment induced a 10-12fold increase in IL-8 production by CRL.1790 cells compared to unstimulated controls at 6 and 12 h(P<0.001).Heat killed E.coli stimulation resulted in a4-5 fold increase in IL-8 compared to the unstimulated control cells at each time point(P<0.001).Both heat killed E.coli and HKC stimulated robust ROS production at 6(P<0.001),and 12 h(P<0.01).Mitochondrial morphologic abnormalities were detected at 6 and12 h based on reduced mitochondrial circularity and decreased mitochondrial membrane potential,P<0.01.Microbial stimulation also induced significant autophagy at 6 and 12 h,P<0.01.Lastly,blocking mitochondrial ROS generation using mitochondrial specific ROS scavenger reversed microbial challenge induced mitochondrial morphologic abnormalities and autophagy.CONCLUSION The findings from this study suggest that CRL.1790cells may be a useful alternative to other colon cancer cell lines in studying the mechanisms of oxidative stress events associated with intestinal inflammatory disorders. | Nandakumar Packiriswamy Kari F Coulson Susan J Holcombe Lorraine M Sordillo | 2017 | World Journal of Gastroenterology2017，23，19： | 3 |
| 3 | The EGF receptor -an essential regulator of multiple epidermal functions显示文摘 | Jost M Kari C Rodeck U | 2000 | Eur J Dermatol2000，10，7： | 1 |
| 4 | Non-linear behavior of a rubber isolator system using fractional derivatives显示文摘 | SJOBERG M KARI L | 2002 | Vehicle System Dynamics2002，37，3： | 1 |
| 5 | Semicarbazide sensitive amine oxidase overexpression hasdual consequences : insulin mimicry and diabetes-like complica-tions显示文摘 | Stolen CM Madanat H Marti L Kari S Yegutkin GG Soriola M | 2004 | FASEB J2004，18，6： | 1 |
| 6 | Pelvic floor muscle injuries 6 weeks post partum—an intra‐ and inter‐rater study显示文摘 | Jette St?r‐Jensen Franziska Siafarikas Gunvor Hilde Ingeborg H. Br?kken Kari B? Marie Ellstr?m Engh | 2013 | Neurourol Urodyn2013，，7： | 1 |
| 7 | Frequency and amplitude dependence of the axial and radial stiffness of carbon- black filled rubber bushings 显示文摘 | Tarrago Garcia M J Kari L | 2007 | Polymer Testing2007，26，： | 1 |
| 8 | Gene expression analysis of Gata3 /- mice by using cDNA microarray technology显示文摘 | Airik Po K rner M Karis A | 2005 | Life Sci2005，76，： | 1 |
| 9 | Targeting the epidermal growth factor receptor in cancer:apoptosis takes center stage显示文摘 | Kari C Chan TO Rocha de Quadros M | 2003 | Cancer Res2003，，： | 1 |
| 10 | Non-linear behavior of a rubber i solator system using fractional derivatives显示文摘 | Sjoberg M Kari L | 2002 | Vehicle System Dynamics2002，37，3： | 1 |
| 11 | Utilisation of a multitubular reactor system for parallel screening of catalysts for ring opening of decalin in continuous mode显示文摘 | Kalle Arve P?ivi M?ki-Arvela Kari Er?nen Marja Tiitta Tapio Salmi Dmitry Yu. Murzin | 2014 | Chemical Engineering Journal2014，，： | 1 |
| 12 | EGF - R dependent regulation of keratinocyte survival 显示文摘 | Rodeck U Jost M Kari C | 1997 | J Cell Sci1997，110，： | 1 |
| 13 | Epidermal growth factor receptor-dependent control of keratinocyte survival and Bcl-xL expression through a MEK-dependent pathway 显示文摘 | Jost M Huggett TM Kari C | 2001 | J Biol Chem2001，276，： | 1 |
| 14 | Classification and prediction of survival in patients with the leukemic phase of cutaneous T cell lymphoma显示文摘 | Kari L Loboda A Nebozhyn M | 2003 | J Exp Med2003，197，11： | 1 |
| 15 | The conversion from cellulose I to cellulose II in NaOH mercerization performed in alcohol-water systems: An X-ray powder diffraction study显示文摘 | Paavo M Manu L Kari R | 2007 | Carbohydr Polym2007，68，1： | 1 |
| 16 | Non-linear Behavior of a Rubber Isolator System Using Fractional Derivatives显示文摘 | Sjoberg M Kari L | 2002 | Vehicle System Dy- namics2002，37，3： | 1 |
| 17 | Current status of ground source heat pumps and underground thermal energy storage in Europe显示文摘 | Sanner B Kary tsas C M endrinos D Rybach L | 2003 | Geothermies2003，3，2： | 1 |
| 18 | Pattern of steroid resistant nephrotic syndrome in children living in the kingdom of Saudi Arabia:a singlecenter study显示文摘 | Kari JA Halawani M Mokhtar G | | 0，，05： | 1 |
| 19 | Oligomeric and f ibrillar species of Amyloid-β peptides显示文摘 | Dahlgren Karie N Manelli Arlene M Stine W Blaine | 2002 | J Biol Chem2002，277，32： | 1 |
| 20 | Peri operatire autotransfusion hemodilution and red cell salvaging 显示文摘 | Kari M | 1991 | Am J Anesth1991，38，： | 1 |
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