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| 1 | Notch signaling controls chondrocyte hypertrophy via indirect regulation of Sox9显示文摘RBPjk-dependent Notch signaling regulates both the onset of chondrocyte hypertrophy and the progression to terminal chondrocyte maturation during endochondral ossification. It has been suggested that Notch signaling can regulate Sox9 transcription, although how this occurs at the molecular level in chondrocytes and whether this transcriptional regulation mediates Notch control of chondrocyte hypertrophy and cartilage development is unknown or controversial. Here we have provided conclusive genetic evidence linking RBPjk-dependent Notch signaling to the regulation of Sox9 expression and chondrocyte hypertrophy by examining tissuespecific Rbpjk mutant(Prx1Cre;Rbpjkf/f), Rbpjk mutant/Sox9 haploinsufficient(Prx1Cre;Rbpjkf/f;Sox9f/1),and control embryos for alterations in SOX9 expression and chondrocyte hypertrophy during cartilage development. These studies demonstrate that Notch signaling regulates the onset of chondrocyte maturation in a SOX9-dependent manner, while Notch-mediated regulation of terminal chondrocyte maturation likely functions independently of SOX9. Furthermore, our in vitro molecular analyses of the Sox9 promoter and Notch-mediated regulation of Sox9 gene expression in chondrogenic cells identified the ability of Notch to induce Sox9 expression directly in the acute setting, but suppresses Sox9 transcription with prolonged Notch signaling that requires protein synthesis of secondary effectors. | Anat Kohn Timothy P Rutkowski Zhaoyang Liu Anthony J Mirando Michael J Zuscik Regis J O'Keefe Matthew J Hilton | 2015 | Bone Research2015，3，3： | 8 |
| 2 | Characterization of the Nanoscale Microstructure of an Immersion Silver on Sputtered Copper显示文摘Silver has grown its attraction of the scientists in microelectronic field recently. Electrochemical deposition of silver onto copper substrates by immersion method has been one of the keen topics. In this study, ethanol was used as the plating solution in which some chemicals were added. The silver deposits were characterized by a field emission scanning electron microscopy (FESEM), an atom force microscope (AFM), a transmission electron microscope (TEM) and a selected area electron diffraction (SAED). It was found that silver immersion using ethanol-based bath has good coverage feature. The highlands of the copper substrate are coated by silver particles whose sizes are around 12 nm, but those sizes at low-lying lands are a little smaller. | TANG Dian WEI Zhe-Liang SHAO Yan-Qun YOU Shao-Xin O'KEEFE Mathew O' KEEFE Thomas | 2005 | Chinese Journal of Structural Chemistry2005，24，8： | 6 |
| 3 | NANO-STRUCTURED SILVER COATING ON COPPER PREPARED IN AN ETHANOL-BASED SOLUTION显示文摘Recently, silver as an electrochemical deposit on copper substrate has been attracting much attention in the microelectronics field. To deposit nano-scale silver particles on copper, immersion plating using cyanide-based baths is commonly used. In this study, non-toxic ethanol was used as the plating solution. Sputtered copper samples were terized by a field emission scanning electron microscope (FE-SEM), an energy dispersive X-ray spectroscope (EDS),and an atomic force microscope (AFM). It was found that the deposited particles are metallic silver. After 3 s immersion,fine particles whose diameters were around 6 nm had covered about 40% of the surface of the copper substrate. After10 s immersion, the copper surface was completely covered by silver particles, the diameters of which have increased to about 10-15 nm. After the whole surface was covered, a dense and smooth silver coating was obtained. | Zheliang Wei Dian Tang Thomas O'Keefe | 2005 | China Particuology2005，3，5： | 6 |
| 4 | Tube feeding,the microbiota,and Clostridium diff icile infection显示文摘Clostridium difficile (C. difficile) is now the leading cause of nosocomial diarrhea in the USA, accounting for 30% of patients with antibiotic-associated diarrhea, 70% of those with antibiotic-associated colitis, and most cases of pseudomembranous colitis. The organism has evolved over the last 8 years to become more virulent and resistant to antimicrobials (NAP1/027 strain) causing a more severe form of the disease that has increased mortality and healthcare costs. While it is generally accepted that the problem results from the overuse of antibiotics, and in particular second and third generation cephalosporins, fluoroquinolones and macrolides, recent studies suggest that acid suppres-sion with proton pump inhibitors (PPIs) may be equally culpable. A further common, but less recognized, etiological factor is the prolonged use of elemental diets. Such diets are totally absorbed within the small intestine and therefore deprive the colonic microbiota of their source of nutrition, namely dietary fiber, fructose oligosaccharides, and resistant starch. The resultant suppression of colonic fermentation leads to suppression of the 'good' bacteria, such as butyrate-producers (butyrate being essential for colonic mucosal health), and bifidobacteria and the creation of a 'permissive' environment for C. difficile colonization and subsequent infection. Based on this analysis, the best chance of suppressing the emerging C. difficile epidemic is to adopt a 3-pronged attack consisting of (1) avoidance of the use of prophylactic antibiotics, (2) the avoidance of prophylactic PPIs, and (3) the conversion of elemental diet feeding to a diet containing adequate indigestible carbohydrate after the first week of critical illness. In this review, we highlight the rising worldwide incidence of C. difficile associated diarrhea and the role played by non-residue diets in destabilizing the colonic microbiota. | Stephen JD O'Keefe | 2010 | World Journal of Gastroenterology2010，16，2： | 4 |
| 5 | Effect of fiber supplementation on the microbiota in critically ill patients显示文摘AIM:To determine tolerance to fiber supplementation of semi-elemental tube feeds in critically ill patients and measure its effect on colonic microbiota and fermentation.METHODS:Thirteen intensive care unit patients receiving jejunal feeding with a semi-elemental diet for predominantly necrotizing pancreatitis were studied.The study was divided into 2 parts:first,short-term (3-9 d)clinical tolerance and colonic fermentation as assessed by fecal short chain fatty acid(SCFA)concentrations and breath hydrogen and methane was measured in response to progressive fiber supplementation increasing from 4 g tid up to normal requirement levels of 8 g tid;second,4 patients with diarrhea were studied for 2-5 wk with maximal supplementation to additionally assess its influence on fecal microbiota quantitated by quantitative polymerase chain reaction (qPCR)of microbial 16S rRNA genes and Human Intestinal Tract Chip(HITChip)microarray analysis.Nearly all patients were receiving antibiotics(10/13)and acid suppressants(11/13)at some stage during the studies.RESULTS:In group 1,tolerance to progressive fiber supplementation was good with breath hydrogen and methane evidence(P=0.008 and P<0.0001,respectively)of increased fermentation with no exacerbation of abdominal symptoms and resolution of diarrhea in 2 of 4 patients.In group 2 before supplementation,fecal microbiota mass and their metabolites,SCFA,were dramatically lower in patients compared to healthy volunteers.From qPCR and HITChip analyses we calculated that there was a 97%reduction in the predominant potential butyrate producers and starch degraders.Following 2-5 wk of fiber supplementation there was a significant increase in fecal SCFA(acetate 28.4±4.1μmol/g to 42.5±3.1μmol/g dry weight,P=0.01;propionate 1.6±0.5 vs 6.22±1.1,P=0.006 and butyrate 2.5±0.6 vs 5.9±1.1,P=0.04)and microbial counts of specific butyrate producers,with resolution of diarrhea in 3 of 4 patients.CONCLUSION:Conventional management of critically ill patients,which includes the use of elemental diets and broad-spectrum antibiotics,was associated with gross suppression of the colonic microbiota and their production of essential colonic fuels,i.e.,SCFA.Our investigations show that fiber supplementation of the feeds has the potential to improve microbiota mass and function,thereby reducing the risks of diarrhea due to dysbiosis. | Stephen JD O'Keefe Junhai Ou James P DeLany Scott Curry Erwin Zoetendal H Rex Gaskins Scott Gunn | 2011 | World Journal of Gastrointestinal Pathophysiology2011，2，6： | 3 |
| 6 | Primary lymphoma of bone:correlation of magneitic resonance imaging features with cyto kine production by tumor cells显示文摘 | Hicks DG Gokan T O'Keefe RJ | | 0，，04： | 2 |
| 7 | The Importance of Fracture Pattern in Guiding Therapeutic Decision-Making in Patients with Hemorrhagic Shock and Pelvic Ring Disruptions显示文摘 | Brian J. Eastridge Adam Starr Joseph P. Minei Grant E. O’Keefe | 2002 | The Journal of Trauma: Injury, Infection, and Critical Care2002，，3： | 2 |
| 8 | Paediatric retinal detachment:aetiology,characteristics and outcomes显示文摘AIM: To provide contemporary data on the aetiology, clinical features and outcomes of paediatric retinal detachment.METHODS: A retrospective review of all those under 16 y who underwent surgical repair for retinal detachment at a single centre between the years 2008 and 2015 inclusive was performed. In each case the cause of retinal detachment, the type of detachment, the presence or absence of macular involvement, the number and form of reparative surgeries undertaken, and the surgical outcome achieved was recorded.RESULTS: Twenty-eight eyes of 24 patients, 15(62.5%) of whom were male and 9(37.5%) of whom were female, their mean age being 11.6 y and range 2-16y developed retinal detachment over the eight year period studied. Trauma featured in the development of retinal detachment in 14(50.0%) cases. Retinal detachment was associated with other ocular and/or systemic conditions in 11(39.3%) cases. A mean of 3.0 procedures with a range of 1-9 procedures per patient were undertaken in the management of retinal detachment. Complex vitrectomy combined with scleral buckling or complex vitrectomy alone were those most frequently performed. Mean postoperative visual acuity was 1.2 log MAR with range 0.0-3.0 log MAR. In 22 of 26(84.6%) cases which underwent surgical repair the retina was attached at last follow-up.CONCLUSION: Aggressive management of paediatric retinal detachment including re-operation increases the likelihood of anatomical success. In cases where the retinal detachment can be repaired by an external approach alone there is a more favourable visual outcome. | Elizabeth McElnea Kirk Stephenson Sarah Gilmore Michael O'Keefe David Keegan | 2018 | International Journal of Ophthalmology(English edition)2018，11，2： | 2 |
| 9 | Visual Interactive Simulation-Histroy,Recent Developments and Major Issues显示文摘 | Robert M O'Keefe | 1987 | Simulation1987，49，3： | 1 |
| 10 | Comments on chemical relationships among Zhamanshinites,Australasian tektites and Henbury impact glass显示文摘 | O'KEEF J A | 1980 | Geochim Cosmochim Acta1980，44，： | 1 |
| 11 | Cyanovirin-N defines a new class of antiviral agent targeting N-linked, high-mannose glycans in an oligosaccharide-specific manner显示文摘 | Bolmstedt AJ O'Keefe BR Shenoy SR | 2001 | Mol Pharinacol2001，59，5： | 1 |
| 12 | Type IV collagen and fibronectin enhance human keratinocyte thymidine incorporation and spreading in the absence of soluble growth factors显示文摘 | Woodley DT Wynn KC O'Keefe EJ | 1990 | Invest Dermatol1990，94，1： | 1 |
| 13 | TGF-beta signaling in ehondrocytes显示文摘 | Li TF O'Keefe RJ Chen D | 2005 | Front Biosei2005，10，： | 1 |
| 14 | Visual outcome and complications of bilateral intraocalar Lens implantat ion in children 显示文摘 | O' keefe M MulvihillA OM Xeoh PL | 2000 | J Cataract Kefract Surg2000，26，11： | 1 |
| 15 | Deposition and characterization of cerium oxide conversion coatings on aluminum alloy 7075-T6 显示文摘 | RIVERA B F OHNSONB Y J O'KEEFE M J | 2004 | Surface and Coatings Technology2004，176，3： | 1 |
| 16 | Preliminaryresults from bench-scale testing of a sulfur-iodine thermo- chemical water-splitting cycle显示文摘 | O' Keefe D Allen C Besenbruch G | 1982 | International Journal of Hydrogen Energy1982，7，5： | 1 |
| 17 | Organizationstrategy and structural differences for radical versus in-cremental innovation显示文摘 | ETTLIE J BRIDGES W O'KEEFE R | 1984 | Management Science1984，30，6： | 1 |
| 18 | Nutritional immuncanoduhtion of acutepan-creatitis显示文摘 | Hegazi RA O'Keefe SJ | 2007 | Curr Gastznenteml Rep2007，9，2： | 1 |
| 19 | Stereopsis in refractive surgery显示文摘 | Kirwan C O′keefe M | 2006 | Am J Ophthalmol2006，142，2： | 1 |
| 20 | Dopamine D1 receptor binding in the anterior cingulated cortex of patients with obsessive-compulsive disorder 显示文摘 | OLVER J S O' KEEFE G JONES G R | 2010 | Psychiatry Res: Neuroimaging2010，183，1： | 1 |
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