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| 1 | Autoantibodies against MMP-7 as a novel diagnostic biomarker in esophageal squamous cell carcinoma显示文摘AIM: To evaluate the diagnostic values of serum autoantibodies against matrix metalloproteinase-7 (MMP-7) in patients with esophageal squamous cell carcinoma (ESCC). METHODS: The MMP-7 cDNA was cloned from ESCC tissues, and MMP-7 was expressed and purified from a prokaryotic system. MMP-7 autoantibodies were then measured in sera from 50 patients with primary ESCC and 58 risk-matched controls, using a reverse capture enzyme-linked immunosorbent assay (ELISA) in which autoantibodies to MMP-7 bound to the purified MMP-7 proteins. In addition, MMP-7 autoantibody levels in sera from 38 gastric cancer patients and from control serum samples were also tested. RESULTS: The optimum conditions for recombinant MMP-7 protein expression were determined as 0.04 mmol/L Isopropyl-β-D-Thiogalactopyranoside (IPTG) induction at 37℃ for four hours. The levels of serum autoantibodies against MMP-7 were significantly higher in patients with ESCC than in the matched-control samples (OD450 = 1.69 ± 0.08 vs OD450 = 1.55 ± 0.10, P < 0.001). The area under the receiver operating characteristic (ROC) curve was 0.87. The sensitivity and specificity for detection of ESCC were 78.0% and 81.0%, respectively, when the OD450 value was greater than 1.65. Although the levels of autoantibodies against MMP-7 were also significantly higher in patients with gastric cancer compared to control samples (OD450 = 1.62 ± 0.06 vs OD450 = 1.55 ± 0.10, P < 0.001), the diagnostic accuracy was less significant than in ESCC patients. The area of ROC curve was 0.75, whereas the sensitivity and specificity were 60.5% and 71.7%, respectively, when the cut-off value of OD450 was set at 1.60. CONCLUSION: Serum autoantibody levels of MMP-7 may be a good diagnostic biomarker for esophageal squamous cell carcinoma. | Jing-Hua Zhou Bin Zhang Kemp H Kernstine Li Zhong | 2011 | World Journal of Gastroenterology2011，17，10： | 8 |
| 2 | Fen1 mutations that specifically disrupt its interaction ~vith PCNA cause aneuploidy-associated cancer显示文摘 | Li Zheng Huifang Dai Muralidhar L Hegde Mian Zhou Zhigang Guo Xiwei Wu Jun Wu Lei Su Xueyan Zhong Sankar Mitra Qin Huang Kemp H Kernstine Gerd P Pfeifer Binghui Shen | 2011 | Cell Research2011，21，7： | 3 |
| 3 | Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells显示文摘 | Cowly S Paterson H Kemp P | 1994 | Cell1994，77，： | 2 |
| 4 | The treatment of earlymorning awakening insomnia with 2 evenings of bright light显示文摘 | Lack L Wright H Kemp K | 2005 | Sleep2005，28，5： | 1 |
| 5 | Molecular mapping of epitopes on melanocyte-specific protein Pmel17 which are recognized by autoantibodies in patients with vitiligo显示文摘 | KEMP E H WATERMAN E A GAWKRODGER D J | 2001 | Clin Exp lmmunol2001，124，3： | 1 |
| 6 | Filtration leukoeytapheresis therapy in the treatment of rheumatoid arthritis patients resistant to or failed with methotrexate显示文摘 | KEMPE K TSUDA H YANG K | 2004 | Ther Apher Dial2004，8，3： | 1 |
| 7 | Distinct Amygdala-autonomic Arousal Profiles in Response to Fear Signals in Healthy Males and Females显示文摘 | WILLIAMS L M BARTON M J KEMP A H | 2005 | Neuroimage2005，，28： | 1 |
| 8 | Pyegenic infection ocuring primary in intervertebral discs显示文摘 | Kemp H B Jackson J W Jeremiah J D | 1973 | J Bone Joint Surg1973，55，4： | 1 |
| 9 | Is caspase-3 inhibition a valid therapeutic strategy in cerebral ischemia显示文摘 | Loetscher H Niederhauser O Kemp J | 2001 | Drug Discov Today2001，6，13： | 1 |
| 10 | Test to determine LC and discriminating dose for macrocycline lactones against the cattle tick Boophilius microplus 显示文摘 | Kemp D H Hughes S | 2001 | Veterinary Parasitology2001，95，： | 1 |
| 11 | Left ventricular function in patients with the anginalsyndrome and normal coronary arteriograms显示文摘 | Kemp H G Jr | 1973 | The AmericanJournal of Cardiology1973，32，3： | 1 |
| 12 | Graphene-SnO, composites for highly efficient photocatalytic degradation of methylene blue under sunlight显示文摘 | Seema H Kemp K C Chandra V et a1 | 2012 | Nanotechnology2012，23，35： | 1 |
| 13 | Comparative eflqcacy and safety of albuterol sulfate Spiros inhaler and albuterol metered-dose inhaler in asthma显示文摘 | Nelson H Kemp JP Bieler S | 1999 | Chest1999，115，2： | 1 |
| 14 | Sur -vival and growth of micro-organisms on air filtration media during initial loading显示文摘 | KEMP P NEUMEISTER-KEMP H LYSEK G | 2001 | Atmospheric Environment2001，35，28： | 1 |
| 15 | NF-κB controls the global proinflammatory response in endothelial cells: evidence for the regulation of a pro atberogenic program显示文摘 | Kempe S Kestler H Lasar A | 2005 | Nucleic Acids Res2005，33，： | 1 |
| 16 | Heat Transfer to Satellite Vehicles Reentering the Atmosphere显示文摘 | Kemp N H Riddell F R | 1957 | Journal of Jet Propulsion1957，27，2： | 1 |
| 17 | A classification of logistics outsourcing levels and their impact on service performance: Evidence from the food processing industry 显示文摘 | H I Hsiao R G M Kemp J G A J van der Vorst S W F (Onno) Omta | 2010 | International Journal of Production Economics2010，，124： | 1 |
| 18 | Minfimally invasive ivor-lewis esophagectomy of the OrVIL device for the EEA显示文摘 | Kemp H Kernstine MD Ph D | 2009 | Intrathoracic Anastomosis2009，7，： | 1 |
| 19 | The GPCOG:a new screening test for dementia designed for general practice显示文摘 | BRODATY H POND D KEMP N M | 2002 | J Am Geriatr Soc2002，50，3： | 1 |
| 20 | Developing a Decision-making Framework for Levels of Logistics Outsourcing in Food Supply Chain Networks显示文摘 | Hsiao H I vander Vorst J G A J Kemp R G M | 2010 | International Journal of Physical Distribution & Logistics Management2010，40，5： | 1 |
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