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| 1 | The production of biobased diamines from renewable carbon sources: Current advances and perspectives显示文摘Bio-based diamines are considered to be a promising alternative to traditional fossil-fuel-based diamines,the important platform chemical for the synthesis of polymer materials.In this review,the current status of the art of the synthesis of aliphatic and aromatic diamines from renewable biomass are considered.In the case of aliphatic diamines,we describe strategies for biologically producing diamines with different carbon numbers including 1,3-diaminopropane,1,4-butanediamine,1,5-pentanediamine,1,6-diaminohexane,1,8-diaminooctane,1,10-diaminodecane,and 1,12-diaminododecane.In addition,aromatic diamines produced from various kinds of renewable biomass,including lignin,cashew nut shell,and terpenoids,are reviewed here.Furthermore,the application of typical diamines in synthesis of polyurethane and polyamide are also reviewed. | Xin Wang Siyuan Gao Jing Wang Sheng Xu Hui Li Kequan Chen Pingkai Ouyang | 2021 | Chinese Journal of Chemical Engineering2021，34，2： | 5 |
| 2 | Study on comparison of different methods to calculating sensitivity index of Jensen model显示文摘Real coded Accelerating Genetic Algorithm(RAGA),Chaos Algorithm(CA)were used to solve the sensitivity index of Jensen model which is one of models of crop water production function.After comparing with the outcome of Least Square Regression(LSR),the result showed that RAGA not only had high accuracy and more effective,but also saved calculating time.The authors provides new effective methods for calculating index of crop water production function. | WANG Kequan FU Qiang JI Fei XU Shuqin | 2007 | Journal of Northeast Agricultural University(English Edition)2007，14，3： | 3 |
| 3 | Ameliorating end-product inhibition to improve cadaverine production in engineered Escherichia coli and its application in the synthesis of bio-based diisocyanates显示文摘Cadaverine is an important C5 platform chemical with a wide range of industrial applications.However,the cadaverine inhibition on the fermenting strain limited its industrial efficiency of the strain.In this study,we report an engineered Escherichia coli strain with high cadaverine productivity that was generated by developing a robust host coupled with metabolic engineering to mitigate cadaverine inhibition.First,a lysine producing E.coli was treated with a combination of radiation(ultraviolet and visible spectrum)and ARTP(atmospheric and room temperature plasma)mutagenesis to obtain a robust host with high cadaverine tolerance.Three mutant targets including HokD,PhnI and PuuR are identified for improved cadaverine tolerance.Further transcriptome analysis suggested that cadaverine suppressed the synthesis of ATP and lysine precursor.Accordingly,the related genes involved in glycolysis and lysine precursor,as well as cadaverine exporter was engineered to release the cadaverine inhibition.The final engineered strain was fed-batch cultured and a titer of 58.7 g/L cadaverine was achieved with a yield of 0.396 g/g,both of which were the highest level reported to date in E.coli.The bio-based cadaverine was purified to>99.6%purity,and successfully used for the synthesis of polyurethane precursor 1,5-pentamethylene diisocyanate(PDI)through the approach of carbamate decomposition. | Xin Wang Xing Guo Jing Wang Hui Li Feng He Sheng Xu Kequan Chen Pingkai Ouyang | 2021 | Synthetic and Systems Biotechnology2021，6，4： | 3 |
| 4 | METTL5 stabilizes c-Myc by facilitating USP5 translation to reprogram glucose metabolism and promote hepatocellular carcinoma progression显示文摘Background:Hepatocellular carcinoma(HCC)is one of the most prevalent cancers in the world,with a high likelihood of metastasis and a dismal prognosis.The reprogramming of glucosemetabolism is critical in the development ofHCC.TheWarburg effect has recently been confirmed to occur in a variety of cancers,including HCC.However,little is known about the molecular biological mechanisms underlying the Warburg effect in HCC cells.In this study,we sought to better understand how methyltransferase 5,N6-adenosine(METTL5)controls the development of HCC and theWarburg effect.Methods:In the current study,quantitative real-time polymerase chain reaction and Western blotting were used to detect the expression of METTL5 in HCC tissues and cell lines.Several different cell models and animal models were established to determine the role of METTL5 in glucose metabolism reprogramming and the underlying molecularmechanism of HCC.Glutathione-S-transferase pulldown,coimmunoprecipitation,RNA sequencing,non-targeted metabolomics,polysome profiling,and luciferase reporter assays were performed to investigate the molecular mechanisms of METTL5 in HCC cells.Results:We discovered that METTL5 drove glucose metabolic reprogramming to promote the proliferation and metastasis of HCC.Mechanistically,upregulation of METTL5 promoted c-Myc stability and thus activated its downstream glycolytic genes lactate dehydrogenase A(LDHA),enolase 1(ENO1),triosephosphate isomerase 1(TPI1),solute carrier family 2 member 1(SLC2A1),and pyruvate kinase M2(PKM2).The c-Box and ubiquitin binding domain(UBA)regions of ubiquitin specific peptidase 5(USP5)binded to c-Myc protein and inhibited K48-linked polyubiquitination of c-Myc.Further study revealed that METTL5 controled the USP5 translation process,which in turn regulated the ubiquitination of c-Myc.Furthermore,we identified cAMP responsive element binding protein 1(CREB1)/P300 as a critical transcriptional regulator ofMETTL5 that promoted the transcription of METTL5 in HCC.In patient-derived tumor xenograft(PDX)models,adenovirus-mediated knockout of METTL5 had a good antitumor effect and prolonged the survival of PDX-bearing mice.Conclusions:These findings point to a novel mechanism by which CREB1/P300-METTL5-USP5-c-Myc controls abnormal glucose metabolism and promotes tumor growth,suggesting that METTL5 is a potential therapeutic target and prognostic biomarker for HCC. | Peng Xia Hao Zhang Haofeng Lu Kequan Xu Xiang Jiang Yuke Jiang Xiangdong Gongye Zhang Chen Jie Liu Xi Chen Weijie Ma Zhonglin Zhang Yufeng Yuan | 2023 | Cancer Communications2023，43，3： | 3 |
| 5 | Efficient production of D-1,2,4-butanetriol from D-xylose by engineered Escherichia coli whole-cell biocataiysts显示文摘We have developed a whole-cell bioconversion system for the production ofD-1,2,4-butanetriol (BT)from renewable biomass.A plasmid pETduet-xylB-yjhG-T7- adhP-T7-mdlC was constructed and transformed to Escherichia coli BL21(DE3)to obtain the whole cells of E.coli BL2I-XYMA capable of bioconversion D-xylose to BT.Then,the factors including carbon sources,nitrogen sources,metal ions,and culture conditions (pH,temperature,IPTG)were identified,and their effects on the wholecell activity for BT production were investigated.To obtain the highest whole-cell activity,the optimal cultivation parameters are:15g·L^-1 yeast extract,5g·L^-1 sucrose, 3g·L^-1 KH2PO4,5g·L^-1 NaCl,3g·L^-1 NH4Cl,0.25g·L^-1 MgSO4·7H2O and 1mL·L^-1 the mixture of trace elements.With the optimized whole cells of E.coli BL21-XYMA,60g·L^-1 of xylose was converted to 28g·L^-1 BT with a molar yield of 66%,which is higher than those reported in the biotechnological system. | Shewei Hu Qian Gao Xin Wang Jianming Yang Nana Xu Kequan Chen Sheng Xu Pingkai Ouyang | 2018 | Frontiers of Chemical Science and Engineering2018，12，4： | 1 |
| 6 | Advance of tolerance engineering on microbes for industrial production显示文摘Industrial microbes have become the core of biological manufacturing,which utilized as the cell factory for production of plenty of chemicals,fuels and medicine.However,the challenge that the extreme stress conditions exist in production is unavoidable for cell factory.Consequently,to enhance robustness of the chassis cell lays the foundation for development of bio-manufacturing.Currently,the researches on cell tolerance covered various aspects,involving reshaping regulatory network,cell membrane modification and other stress response.In fact,the strategies employed to improve cell robustness could be summarized into two directions,irrational engineering and rational engineering.In this review,the metabolic engineering technologies on enhancement of microbe tolerance to industrial conditions are summarized.Meanwhile,the novel thoughts emerged with the development of biological instruments and synthetic biology are discussed. | Siyuan Gao Yang Liao Hao He Huiling Yang Xuewei Yang Sheng Xu Xin Wang Kequan Chen Pingkai Ouyang | 2023 | Synthetic and Systems Biotechnology2023，8，4： | 0 |
| 7 | The production of enantiopure D-lysine from L-lysine by a two-strain coupled system显示文摘The microbial production of D-lysine to replace chemical approach has gained great interest with the rising concerns over the environment.Here,we employed recombinant E.coli strain BL21-LYR with lysine racemase and strain BL-22A-RB-YB with L-lysine monooxygenase and 5-aminovaleramide amidohydrolase to establish a two-strain coupling whole-cell bioconversion system for D-lysine production from L-lysine.To improve the optical purity of D-lysine,the optimal reaction condition for resolution of DL-lysine after the racemization was investigated.The specificity of BL-22A-RB-YB for L^-lysine and the effects of reaction condition on bioconversion efficiency of whole-cell were accordingly determined.Under the optimal condition,a maximum 53.5 g·L^-1 D-lysine and 48.2 g·L^-15-AVA were obtained with yield of 47.4%and 42.3%,respectively,by the microbial racemization and asymmetric degradation process.The final D-lysine enantiomeric excess was over 99%.Meanwhile,a valuable compound 5-aminovaleric acid was synthesized with the production of D-lysine,indicating the economic feasibility of the two-strain coupling system. | Sheng Xu Xiaolu Lu Xin Wang Zhen Gao Kequan Chen Pingkai Ouyang | 2020 | Chinese Journal of Chemical Engineering2020，28，11： | 0 |
| 8 | A triboelectric nanogenerator based oncosmetic fixing powder for mechanicalenergy harvesting显示文摘In this work,we use commercial powder particulates(a cosmetic fixing powder)as triboelectric materials for constructing a triboelectric nanogenerator(CFP-TENG).Through finger pressing,the CFP-TENG generated approximate open-circuit voltage,short-circuit current,and maximum power density values of 1141 V,521μA,and 570.96μW/cm^(2),respectively.Thirty-seven commercial blue LEDs can be easily lit up by the CFP-TENG.Moreover,this TENG,which was designed as a novel palette structure for harvesting mechanical energy from bicycle motion,serves as a self-powered bicycle speed sensor.In addition,the cosmetic fixing powder can be used as an effective material to enhance the triboelectric property of skin.This study provides an effective method for developing a cost-effective TENG without the use of complex surface micro-/nano-texturing. | Kequan Xia Yue Chi Jiangming Fu Zhiyuan Zhu Hongze Zhang Chaolin Du Zhiwei Xu | 2019 | Microsystems & Nanoengineering2019，5，1： | 0 |
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