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| 1 | Underexpression of LATS1 TSG in colorectal cancer is associated with promoter hypermethylation显示文摘AIM:To investigate large tumor suppressor 1 (LATS1 ) expression, promoter hypermethylation, and microsatellite instability in colorectal cancer (CRC).METHODS:RNA was isolated from tumor tissue of 142 CRC patients and 40 colon mucosal biopsies of healthy controls. After reverse transcription, quantitative polymerase chain reaction (PCR) was performed, and LATS1 expression was normalized to expression of the ACTB and RPL32 housekeeping genes. To analyze hypermethylation, genomic DNA was isolated from 44 tumor CRC biopsies, and methylation-specific PCR was performed. Microsatellite instability (MSI) status was checked with PCR using BAT26, BAT25, and BAT40 markers in the genomic DNA of 84 CRC patients, followed by denaturing gel electrophoresis. RESULTS:Decreased LATS1 expression was found in 127/142 (89.4%) CRC cases with the average ratio of the LATS1 level 10.33 ± 32.64 in CRC patients vs 32.85 ± 33.56 in healthy controls. The lowest expression was found in Dukes' B stage tumors and G1 (welldifferentiated) cells. Hypermethylation of the LATS1 promoter was present in 25/44 (57%) CRC cases analyzed. LATS1 promoter hypermethylation was strongly associated with decreased gene expression; methylated cases showed 162× lower expression of LATS1 than unmethylated cases. Although high-grade MSI (mutation in all three markers) was found in 14/84 (17%) cases and low-grade MSI (mutation in 1-2 markers) was found in 30/84 (36%) cases, we found no association with LATS1 expression. CONCLUSION:Decreased expression of LATS1 in CRC was associated with promoter hypermethylation, but not MSI status. Such reduced expression may promote progression of CRC. | Piotr M Wierzbicki Krystian Adrych Dorota Kartanowicz Marcin Stanislawowski Anna Kowalczyk Janusz Godlewski Iwona Skwierz-Bogdanska Krzysztof Celinski Tomasz Gach Jan Kulig Bartlomiej Korybalski Zbigniew Kmiec | 2013 | World Journal of Gastroenterology2013，19，27： | 8 |
| 2 | Study on a relation between estrogen receptor (ESR) gene polymorphism and some pig reproduction performance characters in Poish L and race breed 显示文摘 | Kmiec M Vorak D Vrtkova J | 2002 | Czec J Anim Sci2002，47，： | 1 |
| 3 | Heteroplasmy as a common state of mitochondrial genetic information in the plants and animals显示文摘 | Kmiec B Woloszynska M Janska H | 2006 | Current Genetics2006，50，： | 1 |
| 4 | Pyridoxine-dependent seizures caused by alpha amino atdipic semialdehyde dehydrogenase deficiency: the first polish case with confirmed biochemical and molecular pathology 显示文摘 | Kaczorowska M Kmiec T Jakobs C | 2012 | J Child Neurol2012，23，12： | 1 |
| 5 | Associations between the bovine PPARGC1A gene and milk production traits 显示文摘 | Kowalewska-Luczak I Kulig H Kmiec M | 2010 | Czech Journal of Animal Science2010，55，5： | 1 |
| 6 | Object detection in security applications using dominant edge directions 显示文摘 | Kmiec M Glowacz A | 2015 | Pattern Recognition Letters2015，52，： | 1 |
| 7 | Pyridox-ine-dependent seizures caused by alpha amino atopicsemialdehyde dehydrogenates deficiency: the first pol-ish case with confirmed biochemical and molecular pa-thology 显示文摘 | Kaczorowska M Kmiec T Jakobs C | 2012 | J Child Neurol2012，23，12： | 1 |
| 8 | Shelf acetabuloplasty in the treatment of severe Legg-Calvr-Perthes disease: good outcomes at midterm followup显示文摘 | Grzegorzewski A Synder M Kmiec K | 2013 | Homed Res Int2013，2013，85： | 1 |
| 9 | Development of in vivo tooth EPR for individual radiation dose estimation and screening显示文摘 | Williams BB Dong R Kmiec M | 2010 | Health Phys2010，98，2： | 1 |
| 10 | Pyridoxine-- de- pendent seizures caused by alpha amino atopic semialdehyde dehydrogenates deficiency: the first polish ease with con- firmed biochemical and molecular pathology 显示文摘 | Kaczorowska M Kmiec T Jakobs C | 2012 | J Child Neurol2012，23，12： | 1 |
| 11 | Associa- tions between defensin polymorphism and somatic cell count in milk and milk utility traits in Jersey dairy cows显示文摘 | WOJDAK - MAKSYMIEC K KMIEC M ZUKIEWICZ A | 2006 | J Vet Med A Physiol Pathol Clin Med2006，53，10： | 1 |
| 12 | Pyridoxine-dependent seizures caused by alpha amino adipic semialdehyde dehydrogenase deficiency:the first polish case with confirmed biochemical and molecular pathology显示文摘 | Kaczorowska M Kmiec T Jakobs C | 2008 | J Child Neurol2008，23，12： | 1 |
| 13 | Associations be- tween defensin polymorphism and somatic cell count in milk and milk utility traits in Jersey dairy cows显示文摘 | Wojdak-Maksymiec K Kmiec M Zukiewicz A | 2006 | J Vet Med A Physiol Pathol Clin Med2006，53，10： | 1 |
| 14 | Analysis of relations between polymorphism in steroid 21-hydroxylase gene (CYP21) and quantitative and qualitative characters of boar semen显示文摘 | Kmiec M Ziemak J Dybus A | 2002 | Czech Journal of Animal Science2002，47，5： | 1 |
| 15 | Javelin++:scalability issues in global computing显示文摘 | Neary M O Brydon S P Kmiec P | 2001 | Concurrency :Practice and Experience2001，12，8： | 1 |
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