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| 1 | High‑throughput sequencing view on the magnitude of global fungal diversity显示文摘High-throughput DNA sequencing has dramatically transformed several areas of biodiversity research including mycol-ogy.Despite limitations,high-throughput sequencing is nowadays a predominant method to characterize the alpha and beta diversity of fungal communities.Across the papers utilizing high-throughput sequencing approaches to study natural habitats in terrestrial ecosystems worldwide,>200 studies published until 2019 have generated over 250 million sequences of the primary mycological metabarcoding marker,the nuclear ribosomal internal transcribed spacer 2(ITS2).Here we show that at a 97%sequence similarity threshold,the total richness of non-singleton fungal taxa across the studies published so far is 1.08 million,mostly Ascomycota(56.8%of the taxa)and Basidiomycota(36.7%of the taxa).The Chao-1 estimate of the total extant fungal diversity based on this dataset is 6.28 million taxa,representing a conservative estimate of global fungal species richness.Soil and litter represent the habitats with the highest alpha diversity of fungi followed by air,plant shoots,plant roots and deadwood with Chao-1 predictions,for samples containing 5000 sequences,of 1219,569,392,228,215 and 140 molecular species,respectively.Based on the high-throughput sequencing data,the highest proportion of unknown fungal species is associated with samples of lichen and plant tissues.When considering the use of high-throughput sequenc-ing for the estimation of global fungal diversity,the limitations of the method have to be taken into account,some of which are sequencing platform-specific while others are inherent to the metabarcoding approaches of species representation.In this respect,high-throughput sequencing data can complement fungal diversity predictions based on methods of traditional mycology and increase our understanding of fungal biodiversity. | Petr Baldrian TomášVětrovský Clémentine Lepinay Petr Kohout | 2022 | Fungal Diversity2022，，3： | 8 |
| 2 | Evaluation of the Faces Pain Scale for use with the elderly显示文摘 | Herr KA Mobily PR Kohout F J | 1998 | Clin J Pain1998，14，： | 1 |
| 3 | Evaluation of the FacesPain Scale for use with the elderly显示文摘 | Herr KA? Mobily PR Kohout FJ ^ | 1998 | Clin J Pain1998，14，1： | 1 |
| 4 | Two shorter forms of the CES-D (Center for Epidemiological Studies Depression) depression symptoms index 显示文摘 | Kohout FJ Berkman LF Evans DA | 1993 | J Aging Health1993，5，2： | 1 |
| 5 | Practically Orien- ted Parallel Delaunay Triangulation in Ez for Com- puters with Shared Memory 显示文摘 | Kohout J Kolingerova I Zara J | 2004 | Computers Graphics2004，28，5： | 1 |
| 6 | Practically orien- ted parallel Delaunay triangalation in E2 for computers with shared memory 显示文摘 | KOHOUT J KOLINGEROVA I ZARA J | 2004 | Computers b- Graphics2004，28，5： | 1 |
| 7 | Different effects of two brassinosteroids on growth, auxin and cytokinin content in tobacco callus tissue 显示文摘 | Gaudinova A Sussenbekova H Vojteehova M Kaminek M Eder J Kohout L | 1995 | Plant Growth Regulation1995，17，2： | 1 |
| 8 | beta-arrestin 1 and 2 dif-ferentially regulate heptahelical receptor signaling and trafficking显示文摘 | Kohout T A Lin F T Perry S J | 2001 | Proc Natl Acad Sci USA2001，98，4： | 1 |
| 9 | Augmentation of cardiac contractility mediated by the human beta(3)-adrenergic receptor overexpressed in the hearts of transgenic mice显示文摘 | Kohout TA Takaoka H McDonald PH | 2001 | Circulation2001，104，20： | 1 |
| 10 | The relationship between prescription medication use and ability to ambulate distances after spinal cord injury显示文摘 | Kohout RK Saunders LL Krause JS | 2011 | Arch Phys Med Rehabil2011，92，8： | 1 |
| 11 | Arteriovenous malformation of the head and neck: Natural history and management显示文摘 | Kohout MP Hansen M Pribaz JJ | 1998 | Plast Reeonstr Surg1998，102，3： | 1 |
| 12 | An unusual composite carcinoma of the renal pelvis显示文摘 | Kohout ND Goldman RL | 1973 | J Urol1973，109，4： | 1 |
| 13 | The logic of automata 显示文摘 | B R Gaines L J Kohout | 1976 | Genera/Systems (S0308-1079)1976，23，11： | 1 |
| 14 | Parallel Delaunay tri- angulation in E2 and E3 fo1' computers with shared memory 显示文摘 | KOHOUT J KOLINGEROVA I ZRAR J | 2005 | Parallel Computing2005，31，5： | 1 |
| 15 | Diagnostie possibilities in the management of canine prostatie disorders显示文摘 | Paclikova K Kohout P Vlasin M | 2006 | Vet Med2006，51，1： | 1 |
| 16 | Targeting of cyclic AMP degradation to β2-adrenergic receptors by β-arrestins显示文摘 | Perry SJ Baillie GS Kohout TA | 2002 | Science2002，298，： | 1 |
| 17 | A High- performance Communication Service for Parallel Computing on Distributed DSP Systems显示文摘 | Kohout J George A D | 2003 | Parallel Computing2003，29，： | 1 |
| 18 | The glomus tumor in children显示文摘 | Kohout E Stout AR | | Cancer0，14，： | 1 |
| 19 | Signal transducer and activator of transcription 3 activation is associated with bladder cancer cell growth and survival显示文摘 | Chen C L Cen L Kohout J | | 0，，07： | 1 |
| 20 | Dependence of struc- ture and properties of hard nanocrystalline conductive films MBCN(M = Ti,Zr, Hf) on the choice of metal element显示文摘 | HOUSKA J KOHOUT J MARES P | 2015 | Thin Solid Films2015，586，： | 1 |
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