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| 1 | Treatment of metastatic colorectal carcinomas by systemic inhibition of vascular endothelial growth factor signaling in mice显示文摘AIM: Tumor angiogenesis has been shown to be promoted by vascular endothelial growth factor (VEGF) via stimulating endothelial cell proliferation, migration, and survival. Blockade of VEGF signaling by different means has been demonstrated to result in reduced tumor growth and suppression of tumor angiogenesis in distinct tumor entities. Here, we tested a recombinant adenovirus, AdsFIt1-3, that encodes an antagonistically acting fragment of the VEGF receptor 1 (Flt-1), for systemic antitumor effects in pre-established subcutaneous CRC tumors in mice. METHODS: Murine colorectal carcinoma cells (CT26) were inoculated subcutaneously into Balb/c mice for in vivo studies. Tumor size and survival were determined. 293 cell line was used for propagation of the adenoviral vectors. Human lung cancer line A549 and human umbilical vein endothelial cells were transfected for in vitro experiments. RESULTS: Infection of tumor cells with AdsFlt1-3 resulted in protein secretion into cell supernatant, demonstrating correct vector function. As expected, the secreted sFlt1-3 protein had no direct effect on CT26 tumor cell proliferation in vitro, but endothelial cell function was inhibited by about 46% as compared to the AdLacZ control in a tube formation assay. When AdsFlt1-3 (5×109 PFU/animal) was applied to tumor bearing mice, we found a tumor inhibition by 72% at d 12 after treatment initiation. In spite of these antitumoral effects, the survival time was not improved. According to reduced intratumoral microvessel density in AdsFIt1-3-treated mice, the antitumor mechanism can be attributed to angiostatic vector effects. We did not detect increased systemic VEGF levels after AdsFlt1-3 treatment and liver toxicity was low as judged by serum alanine aminotransferase determination. CONCLUSION: In this study we confirmed the value of a systemic administration of AdsFIt1-3 to block VEGF signaling as antitumor therapy in an experimental metastatic colorectal carcinoma model in mice. | Volker Schmitz Miroslaw Kornek Tobias Hilbert Christian Dzienisowicz Esbher Raskopf Christian Rabe Tilman Sauerbruch Cheng Qian Wolfgang H Caselmann | 2005 | World Journal of Gastroenterology2005，11，28： | 4 |
| 2 | Response to GEMOX plus erlotinib in pancreatic cancer is associated with ERCC 1 overexpression显示文摘 | Thorsten Fuereder Judith Stift Irene Kuehrer Nadja Stranzl Doris Hoeflmayer Gabriela Kornek Werner Scheithauer | 2014 | Eur J Clin Invest2014，，： | 2 |
| 3 | Baseline carcinoembryonic antigen (CEA) serum levels predict bevacizumab‐based treatment response in metastatic colorectal cancer显示文摘 | Gerald W. Prager Kira H. Braemswig Alexandra Martel Matthias Unseld Georg Heinze Thomas Brodowicz Werner Scheithauer Gabriela Kornek Christoph C. Zielinski | 2014 | Cancer Sci2014，，8： | 2 |
| 4 | Phase II trial of two-weekly gemcitabine in patients with advanced biliary?tract cancer显示文摘 | M. Penz G. V. Kornek M. Raderer H. Ulrich-Pur W. Fiebiger A. Lenauer D. Depisch G. Krauss B. Schneeweiss W. Scheithauer | 2001 | Annals of Oncology2001，，2： | 2 |
| 5 | Randomized multicenter phase Ⅱ trial of two different schedules of capecitabine plus oxaliplatin as first-line treatment in advanced colorectal cancer显示文摘 | SCHEITHAUER W KORNEK G V RADERER M | 2003 | J Clin Oncol2003，21，7： | 1 |
| 6 | Organ sparing treatment modalities-which type of treatment for which carcinoma显示文摘 | Kornek GV Selzer E | 2008 | Wien Med Wochenschr2008，158，910： | 1 |
| 7 | Subcutaneous administration of amifostine: A promising therapeutic option in patients with oxaliplatin related peripheral sensitive neuropathy显示文摘 | Penz M Kornek G V Raderer M | 2001 | Ann Oncol2001，12，3： | 1 |
| 8 | Treatment of advanced breast cancer with gemcitabine and vinorelbine plus human granulocyte colony stimulating factor显示文摘 | Haider K Kornek GV Kwasny W | 1999 | Breast Cancer Res Treat1999，55，3： | 1 |
| 9 | Multiple sclerosis and chronic autoimmune encephalomyelitis: a comparative quantitative study of axonal injury in active, inactive, and remyelinated lesions 显示文摘 | Kornek B Storch M K Weissert R | 2000 | Am J Pathol2000，157，1： | 1 |
| 10 | Subcutaneous administration of amifostine: A promising therapeutic option in patients with oxaliplatin-related peripheral sensitive neuropathy显示文摘 | M. Penz G. V. Kornek M. Raderer H. Ulrich-Pur W. Fiebiger W. Scheithauer | 2001 | Annals of Oncology2001，，3： | 1 |
| 11 | Combined radiochemotherapy of locally advanced unresectable pancreatic adenocarcinoma with mitomyc-in C plus 24-hour continuous infusional gemcitabine显示文摘 | Kornek GV Potter R Selzer E | 2001 | Int J Radiat Oncol Biol Phys2001，49，3： | 1 |
| 12 | Treatment of advanced breast cancer with docetaxel and gemcitabine with and without human granulocyte colony-stimulating factor 显示文摘 | Kornek GV Haider K Kwasny W | 2002 | Clin Cancer Res2002，8，： | 1 |
| 13 | Effective combination chemotherapy with paclitaxel and cisplatin with or without human granulocyte colony-stimulating factor and/ or erythropoietin in patients with advanced gastric cancer 显示文摘 | Kornek GV Raderer M Schull B | 2002 | Sr J Cancer2002，6，12： | 1 |
| 14 | Effective combination chemotherapy with bimonthly docetaxel and cisplatin with or without hematopoietic growth factor support in patients with advanced gastroesophageal cancer 显示文摘 | Schull B Kornek GV Schmid K | 2003 | Oncology2003，65，3： | 1 |
| 15 | Natalizumab therapy for highly active pediatric multiple sclerosis 显示文摘 | KORNEK B ABOUL-ENEIN F ROSTASY K | 2013 | JAMA Neurol2013，70，4： | 1 |
| 16 | Multiple sclerosis and chronic autoimmune encephalomyelitis: a comparative quantitative study of axonal injury in active, inactive, and remyelinated lesions显示文摘 | Kornek B Storch MK Weissert R | 2000 | Am J Pathol2000，157，： | 1 |
| 17 | Distribution of a calcium channel subunit in dystrophic axons in multiple sclerosis and experimental autoimmune encephalomyelitis显示文摘 | Kornek b Storch MK Bauer J | 2001 | Brain2001，124，： | 1 |
| 18 | Comparative analysis of CA72-4, CA195 and carcinoembryonic antigen in patients with gastrointestinal malignancies显示文摘 | G. V. Kornek D. Depisch H. R. Rosen E. M. Temsch W. Scheithauer | 1992 | Journal of Cancer Research and Clinical Oncology1992，，4： | 1 |
| 19 | Mitomycin C in combination with capecitabine or biweekly high-dose gemcitabine in patients with advanced biliary tract cancer: A randomised phase Ⅱ trial 显示文摘 | Kornek GV Schuell B Laengle F | 2004 | Ann Oncol2004，15，： | 1 |
| 20 | New loci regulating rat myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomylitis 显示文摘 | Kristina Becanovic Erik Wallstrom Barbara Kornek | 2003 | Immunology2003，17，4： | 1 |
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