|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Engineering osteoblastic metastases to delineate the adaptive response of androgen-deprived prostate cancer in the bone metastatic microenvironment显示文摘While stromal interactions are essential in cancer adaptation to hormonal therapies,the effects of bone stroma and androgen deprivation on cancer progression in bone are poorly understood.Here,we tissue-engineered and validated an in vitro microtissue model of osteoblastic bone metastases,and used it to study the effects of androgen deprivation in this microenvironment.The model was established by culturing primary human osteoprogenitor cells on melt electrowritten polymer scaffolds,leading to a mineralized osteoblast-derived microtissue containing,in a 3D setting,viable osteoblastic cells,osteocytic cells,and appropriate expression of osteoblast/osteocyte-derived mRNA and proteins,and mineral content.Direct co-culture of androgen receptordependent/ independent cell lines (LNCaP,C4-2B,and PC3) led cancer cells to display functional and molecular features as observed in vivo.Co-cultured cancer cells showed increased affinity to the microtissues,as a function of their bone metastatic potential.Cocultures led to alkaline phosphatase and collagen-I upregulation and sclerostin downregulation,consistent with the clinical marker profile of osteoblastic bone metastases.LNCaP showed a significant adaptive response under androgen deprivation in the microtissues,with the notable appearance of neuroendocrine transdifferentiation features and increased expression of related markers (dopa decarboxylase,enolase 2).Androgen deprivation affected the biology of the metastatic microenvironment with stronger upregulation of androgen receptor,alkaline phosphatase,and dopa decarboxylase,as seen in the transition towards resistance.The unique microtissues engineered here represent a substantial asset to determine the involvement of the human bone microenvironment in prostate cancer progression and response to a therapeutic context in this microenvironment. | Nathalie Bock Ali Shokoohmand Thomas Kryza Joan Rohl Jonelle Meijer Phong A. Tran Colleen C. Nelson Judith A. Clements Dietmar W. Hutmacher | 2019 | Bone Research2019，7，2： | 2 |
| 2 | Fully automated fluoro- choline synthesis in the TracerLab MX FDG Co- incidence synthesizer显示文摘 | David Kryza Vincent Tadino Maria Azzurra Filannino | 2008 | Nuclear Medicine and Biology2008，35，2： | 1 |
| 3 | High-pressure Granu- lites from the Sudetes (South-west Poland) :Evidence of Crustal Subduction and Collisional Thickening in the Variscan Belt显示文摘 | KRYZA R PIN C VIELZEUF D | 1996 | Journal of Metamorphic Geology1996，14，4： | 1 |
| 4 | The Central-Sudetic ophiolites(SW Poland):petrogenetic issues,geochronology and palaeotectonic implications显示文摘 | Kryza R Pin Ch | | 0，，： | 1 |
| 5 | Fully automated fluorocholine synthesis in the traceLab MX FDG Coincidence synthesizer显示文摘 | Kryza D Tadino V Filannino MA | 2008 | Nucl Med Biol2008，35，2： | 1 |
| 6 | The effect of emission from coal combustion in nonindustrial sources on deposition of sulfur and oxidized nitrogen in Poland显示文摘 | KRYZA M WERNER M BAS M | 2010 | Journal of the Air and Waste Management Association2010，60，7： | 1 |
| 7 | Fine-resolution modeling of concentration and deposition of nitrogen and sulphur compounds for Poland -Application of the FRAME model显示文摘 | KRYZA M BLAS M DORE AJ | 2010 | Archives of Environmental Protection2010，36，1： | 1 |
| 8 | Influence of methodof packingon physicochemical and textural changes in atlantic herring during frozen storage显示文摘 | Grzegorz Szczepanik Krzysztof Kryza Marta Ostrowska | | 0，，04： | 1 |
| 9 | Fully automated fluorocholine synthesis in the tracerlab MX FDG coincidence synthesizer显示文摘 | Kryza D Tadino V Filannino MA | 2008 | Nucl Med Biol2008，35，2： | 1 |
| 10 | Fully automated fluorocholine synthesis in the TracerLab MX FDG Coincidence synthesizer 显示文摘 | Kryza D Tadino V Filannino MA | 2008 | Nucl Med Biol2008，35，2： | 1 |
| 11 | “舌尖体”速成秘籍显示文摘话说公元2012至2014年间,纪录片《舌尖上的中国》风靡华夏大地,将“吃货”这一群体推上了时代的风口浪尖,并在民间催生了一种新兴文体——“舌尖体”。一时间,啤酒、炸鸡、拉面、汉堡纷纷揭竿而起,力图借助文字实现自己变身“高大上”美食的夙愿。那么这化腐成奇之功如何才能炼成呢?特此奉上这篇“舌尖体”速成秘籍,请诸君细细体味。 | Andy Kryza 子昊 | 2014 | 新东方英语(中英文版)2014，，11： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员