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| 1 | Establishment and characterization of four human hepatocellular carcinoma cell lines containing hepatitis B virus DNA显示文摘INTRODUCTIONHepatocelularcarcinoma(HCC)isoneofthemostprevalentmalignantdiseasesencounteredintheworld,kilingupto1milionpeoplea... | Jae Ho Lee 1, Ja Lok Ku 1, Young Jin Park 1,2 , Kuhn Uk Lee 2, Woo Ho Kim 3 and Jae Gahb Park 1,2 | 1999 | World Journal of Gastroenterology1999，5，4： | 28 |
| 2 | Characterization of pancreatic stem cells derived from adult human pancreas ducts by fluorescence activated cell sorting显示文摘瞄准:用没有浆液的、调节媒介从胰管的人的成年纸巾孤立通常认为的胰腺的干细胞(PSC ) 。这些 PSC 的表面显型的描述被流动血细胞计数分析。为胰腺的系和在这些 PSC 的贝它房间区别的能力的潜力也被评估。方法:由使用与必要生长因素补充的没有浆液的媒介,我们试图孤立被报导了表示巢在的通常认为的 PSC 和 pdx-1。Matrigel (TM ) 被采用评估孤立的房间的微分能力。染色的染色的双硫腙,胰岛素内容 / 分泌物测量,和免疫组织化学被用来监视区别。荧光(FACS ) 激活的房间排序被用来检测通常认为的 PSC 的表型的标记。结果:像锭子的房间的单层被栽培。通常认为的 PSC 表示了 pdx-1 和巢在里面。他们也能区分进胰岛素 -- , glucagon- ,和 somatostatin 积极的房间。在 PSC 的表型的标记的光谱被调查;当用人的骨头导出髓的干细胞作为比较级试验时,类似被揭示,例如 CD29, CD44, CD49,半数治愈量, CD51, CD62E, PDGFR-alpha, CD73 (SH2 ) , CD81, CD105 (SH3 ) 。结论:在这研究,我们成功地由使用没有浆液的媒介从成年的人的胰腺的管孤立 PSC。这些 PSC 不仅表示的巢在和 pdx-1 而且对间充质的干细胞可归因的展出标记。尽管工作被需要阐明这些房间的角色,这些 PSC 的申请可能是为糖尿病的治疗学的策略。 | Han-Tso Lin Shih-Hwa Chiou Chung-Lan Kao Yi-Ming Shyr Chien-Jen Hsu Yin-Wen Tarng Larry L-T Ho Ching-Fai Kwok Hung-Hai Ku | 2006 | World Journal of Gastroenterology2006，12，28： | 15 |
| 3 | Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method显示文摘AIM: To enhance the differentiation of insulin producing cell (IPC) ability from embryonic stem (ES) cells in vitro. METHODS: Four-day embryoid body (EB)-formatted ES cells were dissociated as single cells for the followed plasmid DNA delivery. The use of NucleofectorTM electroporator (Amaxa biosystems, Germany) in combination with medium-contained G418 provided a high efficiency of gene delivery for advanced selection. Neucleofected cells were plated on the top of fibronectincoated Petri dishes. Addition of Ly294002 and raised the glucose in medium at 24 h before examination.The differentiation status of these cells was monitored by semi-quantitative PCR (SQ-PCR) detection of the expression of relative genes, such as oct-4, sox-17, foxa2, mixl1, pdx-1, insulin 1, glucagons and somatostatin. The percentage of IPC population on d 18 of the experiment was investigated by immunohistochemistry (IHC), and the content/secretion of insulin was estimated by ELISA assay. The mice with severe combined immunodeficiency disease (SCID) pretreated with streptozotocin (STZ) were used to eliminate plasma glucose restoration after pax4+ ES implantation. RESULTS: A high efficiency of gene delivery was demonstrated when neucleofection was used in the present study; approximately 70% cells showed DsRed expression 2 d after neucleofection. By selection of medium-contained G418, the percentage of DsRed expressing cells kept high till the end of study. The pancreatic differentiation seemed to be accelerated by pax4 nucleofection. When compared to the group of cells with mock control, foxa2, mixl1, pdx1, higher insulin and somatostatin levels were detected by SQ-PCR 4 d after nucleofection in the group of pax4 expressing plasmid delivery. Approximately 55% of neucleofected cells showed insulin expression 18 d after neucleofection, and only 18% of cells showed insulin expression in mock control. The disturbance was shown by nucleofected pax4 RNAi vector; only 8% of cells expressed insulin 18 d after nucleofection. A higher IPC population was also detected in the insulin content by ELISA assay, and the glucose dependency was demonstrated in insulin secretion level. In the animal model, improvement of average plasma glucose concentration was observed in the group of pax-4 expressed ES of SCID mice pretreated with STZ, but no significant difference was observed in the group of STZ-pretreated SCID mice who were transplanted ES with mock plasmid. CONCLUSION: Enhancement of IPC differentiation from EB-dissociated ES cells can be revealed by simply using pax4 expressing plasmid delivery. Not only more IPCs but also pancreatic differentiation-related genes can be detected by SQ-PCR. Expression of relative genes, such as foxa 2, mixl 1, pdx-1, insulin 1 and somatostatin after nucleofection, suggests that pax4 accelerates the whole differentiation progress. The higher insulin production with glucose dependent modulation suggests that pax4 expression can drive more mature IPCs. Although further determination of the entire mechanism is required, thepotential of pax-4-nucleofected cells in medical treatment is promising. | Han-Tso Lin Chung-Lan Kao Kun-Hsiung Lee Yuh-Lih Chang Shih-Hwa Chiou Fu-Ting Tsai Tung-Hu Tsai Dey-Chyi Sheu Larry LT Ho Hung-Hai Ku | 2007 | World Journal of Gastroenterology2007，13，11： | 10 |
| 4 | Efficacy of transient elastography in screening for large esophageal varices in patients with suspicious or proven liver cirrhosis显示文摘 | Huay‐Min WANG Gin‐Ho LO Wen‐Chi CHEN Ping‐I HSU Hsien‐Chung YU Chiun‐Ku LIN Hoi‐Hung CHAN Wei‐Lun TSAI Feng‐Woei TSAY Tsan‐Ping LIU E‐Ming WANG Kwok‐Hung LAI | 2012 | Journal of Digestive Diseases2012，，8： | 2 |
| 5 | Analysis of differential gene expression in auricular lymph nodes draining skin exposed to sensitizers and irritants显示文摘 | KU HO JEONG SH KANG HG | 2008 | Toxicol Lett2008，177，1： | 1 |
| 6 | Gene expression profiles and pathways in skin inflammation induced by three different sen- sitizers and an irritant显示文摘 | KU HO JEONG SH KANG HG | 2009 | Toxicol Lett2009，190，2： | 1 |
| 7 | Supramolecular Structure of fl-Cyclodextrin and Poly(ethylene oxide)- block-poly(propylene oxide)-block-poly(ethylene oxide) Inclusion Complexes显示文摘 | Tsai CC Leng SW Jeong KU Van Horn RM Wang CL Zhang WB Graham M J Huang J Ho RM Chen YM Lotz B Stephen Cheng ZD | 2010 | Macromolecules2010，43，22： | 1 |
| 8 | Global supplier selection using fuzzy analytic hierarchy process and fuzzy goal programming显示文摘 | KU C-Y Chang C-T HO H-P | 2010 | Qual Quant2010，，44： | 1 |
| 9 | Occurrence of virulence deter- minants in fecal Enterececcus faecalis isolated from pigs and chickens in Korea显示文摘 | Hwang l'Y Lira SK Ku HO | 2011 | J Microbiol Biotechnol2011，21，12： | 1 |
| 10 | Ultrasonic inspection of long steel pipes using Lamb waves显示文摘 | Moon Ho Park In Sup Kim Young Ku yoon | 1996 | NDT & E International1996，29，1： | 1 |
| 11 | Properties of electron beam welded SAF 2205 duplex stainless steel显示文摘 | Ku J S Ho N J Tjong S C | 1997 | Journal of Materials processing Technology1997，63，： | 1 |
| 12 | Analysis of synthetic anti-diabetic drugs in adulterated traditional Chinese medicines by highperformance capillary electrophoresis显示文摘 | Ku Y R Chag L Y Ho L K | 2003 | J Pharm Biomed Anal2003，33，2： | 1 |
| 13 | Surface plasmon excitation in semitransparent inverted polymer photovoltaic devices and their applications as label-free optical sensors显示文摘Herein,we report on surface plasmon(SP)-sensitive semitransparent inverted polymer photovoltaic(PV)devices that are based on multilayered material systems consisting of poly(3-hexylthiophene):fullerene-derivative bulk-heterojunction PV layers and thin gold or silver anodes.We demonstrate that these PV devices allow the simultaneous generation of both electrical power and SPs on their anodes for photoexcitation just above the optical absorption edge of the PV layers,resulting not only in attenuated total reflection,but also in attenuated photocurrent generation(APG)under the SP resonance(SPR)condition.Moreover,we also confirm that the biomolecular interaction of biotin–streptavidin on the PV devices can be precisely detected via apparent SPR angle shifts in the APG spectra,even without the need for complex attenuated total reflection configurations.We highlight our view that APG measurements made using these PV devices show great potential for the development of future generations of compact and highly sensitive SPR-based optical sensors. | Byoungchoo Park Soo Hong Yun Chan Youn Cho Young Chan Kim Jung Chul Shin Hong Goo Jeon Yoon Ho Huh Inchan Hwang Ku Youn Baik Young In Lee Han Sup Uhm Guang Sup Cho Eun Ha Choi | 2014 | Light(Science & Applications)2014，3，1： | 1 |
| 14 | Harnessing the opencircuit voltage via a new series of Ru(Ⅱ) sensitizers bearing (iso-)quinolinyl pyrazolate ancillarie显示文摘 | Wu K L Ku W P Clifford J N Palomares E Ho S T Chi Y Liu S H Chou P T Nazeeruddin M K Gr(a)tzel M | | 0，，： | 1 |
| 15 | Diffusion Tensor Magnetic Resonance Imaging of Microstructural Abnormalities in Children with Brain Injury显示文摘 | Zee Ihn Lee Woo Mok Byun Sung Ho Jang Sang Ho Ahn Han Ku Moon Yongmin Chang | 2003 | American Journal of Physical Medicine & Rehabilitation2003，，7： | 1 |
| 16 | Effects of bagging material on fruit coloration and quality of'Janghowon Hwangdo'peach显示文摘 | Kim Young Ho Kim Hag Hyun Youn Cheol Ku | 2008 | Acta Horticuhurae2008，772，： | 1 |
| 17 | Properties of electron beam welded SAF 2205 duplex stainless steel 显示文摘 | Ku J S Ho N J Tjong S C | 1997 | Journal of Materials Processing Technology1997，63，13： | 1 |
| 18 | Short- term Load Forecasting of Taiwan Power System Using a Knowledge- based Expert System 显示文摘 | Ho Ku -Long Hsu Yuan- Yih Lee C E | 1990 | IEEE PWRS1990，5，4： | 1 |
| 19 | Progressive impairment of erythroeyte deformability as indicator of microangiopathy in type 2 diabetes mellitu显示文摘 | Shin S Ku YH Ho JX | 2007 | Clin Hemorheol Microcirc2007，36，3： | 1 |
| 20 | Short term load forecasting of Tai wan power system using knowledge based expert system显示文摘 | Ho Ku Long Hsu Yuan Yih Lee C E | | 0，，04： | 1 |
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