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| 1 | AZF microdeletions associated with idiopathic and non idiopathic cases with cryptorchidism and varicocele显示文摘Aim:To identify submicroscopic interstitital deletions in azoospermia factor (AZF) loci in idiopathic and non-idiopathic cases of male infertility in Indians.Methos:One hundred and twenty two infertile males with oligozoospermia or azoospermia were included in this study.Semen analysis was done to determine the sperm density,i.e.,normospermia(>20million/mL),oligozoospermia(<20million/mL) or azoospermia.They were subjected to detailed clinical examination and endocrinological and cytogenetic study.Thirty G-banded metaphases were analyzed in the 122 cases and polymerase chain reaction (PCR) microdeletion analysis was done in 70 cytogenetically normal subjects.For this genomic DNA was extracted using peripheral blood.The STS primers tested in each case were sY84,sY86(AZFa);sY127,sY134(AZFb);sY254,sY255(AZFc).PCR amplifications found to be negative were repeated at least 3 times to confirm the deletion of a given marker.The PCR products were analyzed on a 1.8% agarose gel.Results:Eight of the 70 cades(11.4%) showed deletion of at least one of the STS markers.Deletions were detected in cases with known and unknown aetiology with bilateral severe testiculopathy and also in cryptorchid and varicocele subjects.Conclusion.AZF microdeletions were seen in both idiopathic and non-idiopathic cases with cryptorchidism and varicocele.The finding of a genetic aetiology in infertile men with varicocele and cryptorchidism suggests the need for molecular screening in non-idiopathic cases. | Rima Dada N.P. Gupta K. Kucheria | 2002 | Asian Journal of Andrology2002，4，4： | 22 |
| 2 | Y chromosome microdeletions in azoospermic patients with Klinefelter's syndrome显示文摘瞄准:与 Klinefelter 的症候群(KFS ) 在 azoospermic 病人学习 Y 微删除的出现。方法:血和精液样品与 KFS 从 azoospermic 病人被收集(n = 14 ) 并且证明富饶的人的一个控制组(n = 13 ) 。精液分析根据世界卫生组织被做() 指南。血样品为 karyotyping,荧光灯的原位杂交(鱼) 和由放射性免疫测定刺激荷尔蒙(FSH ) 的血浆滤泡的测量被处理。决定 Y 微删除, 16 顺序的聚合酶链反应(PCR ) 标注了地点(圣) ,三基因(DFFRY, XKRY 和 RBM1Y ) 在孤立的 genomic DNA 上被执行。阴囊的好针渴望细胞学(FNAC ) 在选择情况中被做。结果:跨越一个 and AZFb 部位与 KFS 在 14 个 azoospermic 病人中的四个被发现的精子缺乏因素(AZF ) 的 Y 微删除。染色质组型和鱼分析表明显示出 Y 微删除的四个盒子,三个盒子有一 47, XXY/46, XY 染色体的模式和一个盒子有一 46, XY/47, XXY/48, XXXY/48, XXYY 染色体的模式。有 Y 微删除的一件样品的阴囊的 FNAC 揭示了形态学的 Sertoli 房间唯一的类型。然而,没有 Y 微删除在任何 13 个肥沃的人被观察。有 KFS 的所有病人提高了血浆 FSH 层次。结论:有 KFS 的病人可以怀有 Y 微删除并且为这些屏蔽应该是他们的诊断病情的检查的部分,特别地在那些考虑帮助了繁殖技术。 | Anurag Mitra Rima Dada Rajeev Kumar Narmada Prasad Gupta Kiran Kucheria Satish Kumar Gupta | 2006 | Asian Journal of Andrology2006，8，1： | 8 |
| 3 | Higher frequency of Yq microdeletions in sperm DNA as compared to DNA isolated from blood显示文摘瞄准:决定删除的 Yq 微删除频率和 loci 是否在不同胚胎学的起源的二纸巾(血和精子) 是类似的。方法:现在的学习包括了 52 个不肥沃的 oligozoospermic 盒子。在每种情况中, DNA 从血和精子被孤立,微删除分析从 genomic DNA 被做的聚合酶链反应(PCR ) 从两个孤立纸巾。PCR 产品在 1.8% 统帅玫瑰胶化上被分析。发现否定的 PCR 扩大被重复至少三次证实一个给定的标记的删除。结果:仅仅 1 个盒子在血 DNA 怀有微删除,而 4 个盒子在精子 DNA 怀有微删除。结论:Yq 微删除的频率作为与血相比在细菌房间是更高的。为帮助繁殖生产技术(艺术) 作为不肥沃的夫妇的多数抉择, Yq 微删除从细菌房间屏蔽是重要的理解不孕的基因基础,提供全面建议和大多数改编治疗学给不肥沃的夫妇。 | Rima Dada Rakesh Kumar M. B. Shamsi Rajeev Kumar Kiran Kucheria Raj K. Sharma Satish K. Gupta Narmada P. Gupta | 2007 | Asian Journal of Andrology2007，9，5： | 6 |
| 4 | Molecular screening for Yq Microdeletion in men with idiopathic oligozooospermia and zooaperia 显示文摘 | Dada R Gupta NP Kucheria K | 2003 | Iosci2003，28，2： | 1 |
| 5 | Molecular screening for Yq microdeletion in men with idio- pathie o permia and azoospermia 显示文摘 | Dada R Gupta NP Kucheria K | 2003 | Jour- nal of Biosieiences2003，28，2： | 1 |
| 6 | Molecular screening for Yq microdeletion in men with idiopathic oligozoospermia and azoospermia显示文摘 | Rima Dada N. P. Gupta K. KucherIA | 2003 | Journal of Biosciences2003，，2： | 1 |
| 7 | Molecular screening forY qmicrodeletion in men with idiopathic oligozoospermia and azoospermia显示文摘 | Dada R Gupta NP Kucheria K | | 0，，02： | 1 |
| 8 | Molecular screening for Yq microdeletion in men with idiopathic oligozoosper mia and azoosprmia显示文摘 | GUPTA NP KUCHERIA K | 2003 | Biosci2003，28，： | 1 |
| 9 | Laparoseopie management of eryptorehidism in adults 显示文摘 | Kucheria R Sabai A Sami TA | 2005 | Eur Urol2005，48，3： | 1 |
| 10 | Molecular screening for Yq microdeletion in men with idiopathic oligozoospermia and azoospermia 显示文摘 | Dada R Gupta NP Kucheria K | 2003 | J Biosci2003，28，2： | 1 |
| 11 | Molecular screening for Yq microdeletion in men with idiopathic oligozoospermia and azoospermia 显示文摘 | Dada R Gupta NP Kucheria K | 2003 | J Biosci2003，28，2： | 1 |
| 12 | Prenatal detection of aneuploidies using fluorescence in stiu hybridization:A preliminary experience in an Indian set up显示文摘 | Jobanputra V Roy KK Kucheria K | 2002 | J Biosci2002，27，2： | 1 |
| 13 | Molecular screening for Yq microdeletion in men with idiopathic oligozoospermia and azoospermia 显示文摘 | DADA R G UPTA NP KUCHERIA K | 2003 | J Biosci2003，28，： | 1 |
| 14 | Molecular screening for Yq microdeletion in men with idiopathic oligozoospermia and azoospermia显示文摘 | DADA R GUPTA N P KUCHERIA K | 2003 | J Biosci2003，28，2： | 1 |
| 15 | Molecular screening for Yq microdeletion in men with idiopathic oligozoospermia and azoospermia显示文摘 | Dada R Gupta NP Kucheria K | 2003 | J Biosci2003，28，2： | 1 |
| 16 | Molecular screening for Yq microdeletion in men with idiopathic oligozoospermia and azoospermia显示文摘 | Dada R Gupta NP Kucheria K | 2003 | J Biosci2003，28，： | 1 |
| 17 | Semen cryoconservation in men with AZFe mierodeletion 显示文摘 | Dada R Gupta NP Kucheria K | 2003 | Clin Genet2003，64，1： | 1 |
| 18 | Molecular screening for Yq Microdeletion in men with idiopathicoligozooospermia and zooaperia显示文摘 | Dada R Gupta NP Kucheria K | 2003 | Iosci2003，28，2： | 1 |
| 19 | Effect of phenytoin on se-men显示文摘 | Taneja N Kucheria K Jain S | 1994 | Epilepsia1994，35，： | 1 |
| 20 | Molecular screening for Yq microdeletion in men with idiopathic oligozoospermia and azoospermia显示文摘 | Dada R Gupta NP Kucheria K | 2003 | J Biosci2003，28，2： | 1 |
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