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| 1 | The Tea Tree Genome Provides Insights into Tea Flavor and Independent Evolution of Caffeine Biosynthesis显示文摘茶是世界有巨大的经济、药用、文化的重要性的最旧、很流行的包含咖啡因的饮料。这里,我们在场第一个高质量的核苷酸序列充满重复(80.9%) ,栽培的茶树山茶 sinensis 的 3.02-Gb 染色体。我们证明茶树的一种非常地大的染色体尺寸从一些 LTR retrotransposon 家庭的慢、稳定、长期的扩大被结果。除了一个最近的整个染色体的复制事件,基因的系特定的扩大把新陈代谢的生合成与 flavonoid 联系了被发现,它提高 catechin 生产,萜烯酶激活,和应力公差,为茶风味和改编的重要特征。我们相对可可子和咖啡表明一个独立人士和茶咖啡因合成小径的快速的进化。在 25 山茶种类之中的比较研究表明那更高的表情大多数 flavonoid- 和咖啡因铺平 -- 然而并非 theanine 相关的基因贡献 catechins 和咖啡因的增加的生产并且因此提高处理茶的适用性和茶质量。这些新奇调查结果为进一步的 metabolomic 和典型生合成小径的功能的 genomic 精炼铺平道路并且将帮助开发将最后满足并且吸引世界范围的更多的茶喝酒者的茶风味的一个更多样化的集合。 | En-Hua Xia Hai-Bin Zhang Jun Sheng Kui Li Qun-Jie Zhang Changhoon Kim Yun Zhang Yuan Liu Ting Zhu Wei Li Hui Huang Yan Tong Hong Nan Cong Shi Chao Shi Jian-Jun Jiang Shu-Yan Mao Jun-Ying Jiao Dan Zhang Yuan Zhao You-Jie Zhao Li-Ping Zhang Yun-Long Liu Ben-Ying Liu Yue Yu Sheng-Fu Shao De-Jiang Ni Evan E. Eichler Li-Zhi Gao | 2017 | Molecular Plant2017，10，6： | 113 |
| 2 | The Chromosome-Based Rubber Tree Genome Provides New Insights into Spurge Genome Evolution and Rubber Biosynthesis显示文摘The rubber tree,Hevea brasiliensis,produces natural rubber that serves as an essential industrial raw material.Here,we present a high-quality reference genome for a rubber tree cultivar GT1 using single-molecule real-time sequencing(SMRT)and Hi-C technologies to anchor the~1.47-Gb genome assembly into 18 pseudochromosomes.The chromosome-based genome analysis enabled us to establish a model of spurge chromosome evolution,since the common paleopolyploid event occurred before the split of Hevea and Manihot.We show recent and rapid bursts of the three Hevea-specific LTR-retrotransposon families during the last 10 million years,leading to the massive expansion by~65.88%(~970 Mbp)of the whole rubber tree genome since the divergence from Manihot.We identify large-scale expansion of genes associated with whole rubber biosynthesis processes,such as basal metabolic processes,ethylene biosynthesis,and the activation of polysaccharide and glycoprotein lectin,which are important properties for latex production.A map of genomic variation between the cultivated and wild rubber trees was obtained,which contains~15.7 million high-quality single-nucleotide polymorphisms.We identified hundreds of candidate domestication genes with drastically lowered genomic diversity in the cultivated but not wild rubber trees despite a relatively short domestication history of rubber tree,some of which are involved in rubber biosynthesis.This genome assembly represents key resources for future rubber tree research and breeding,providing novel targets for improving plant biotic and abiotic tolerance and rubber production. | Jin Liu Cong Shi Cheng-Cheng Shi Wei Li Qun-Jie Zhang Yun Zhang Kui Li Hui-Fang Lu Chao Shi Si-Tao Zhu Zai-Yun Xiao Hong Nan Yao Yue Xun-Ge Zhu Yu Wu Xiao-Ning Hong Guang-Yi Fan Yan Tong Dan Zhang Chang-Li Mao Yun-Long Liu Shi-Jie Hao Wei-Qing Liu Mei-Qi Lv Hai-Bin Zhang Yuan Liu Ge-Ran Hu-tang Jin-Peng Wang Jia-Hao Wang Ying-Huai Sun Shu-Bang Ni Wen-Bin Chen Xing-Cai Zhang Yuan-Nian Jiao Evan E.Eichler Guo-Hua Li Xin Liu Li-Zhi Gao | 2020 | Molecular Plant2020，13，2： | 15 |
| 3 | IL-37b gene transfer enhances the therapeutic efficacy of mesenchumal stromal cells in DSS- induced colitis mice显示文摘瞄准:调查是否 IL-37b 基因的转移,天生、适应的免疫的一个最新识别的禁止者,能在煽动性的肠疾病( IBD )改进 mesenchumal stromal 房间( MSC )的治疗学的功效 .Methods :在有活跃 ulcerative 大肠炎( UC )的病人的 biopsied 标本的 IL-37 的表示用 RT-PCR 和 immunohistochemistry 被检测。老鼠与 3% 葡聚糖硫酸盐钠(决策支持系统) 被对待 8 天导致大肠炎。在决策支持系统治疗前,老鼠与 MSC, MSC-eGFP 或 MSC-IL37b 被注射。他们的身体重量每天被测量,并且结肠和脾在 d 上被收获 10 为病理学、生物化学的 analyses.Results : 在有活跃 UC 的病人,的 biopsied 标本, IL-37 的表示戏剧性地在煽动的 mucosa 被提高,主要在上皮的房间和渗透的有免疫力的房间。比作 MSC-eGFP 或 MSC, MSC-IL37b 管理显著地稀释了身体重量和冒号长度减小,并且减少在导致决策支持系统的大肠炎老鼠的组织学的分数。而且, MSC-IL37b 管理在老鼠在脾的 CD4+ T 房间之中象规章的 T 房间(Tregs ) 的百分比一样在全部的脾的 mononuclear 房间之中增加了导出 myeloid 的 suppressor 房间(MDSC ) 的百分比。而且, MSC-IL37b 管理增加了 IL-2+ 房间并且减少 IFN-γ在脾的 CD4+ T cells.Conclusion 之中的 + 房间: IL-37 涉及 UC 的 pathophysiology。IL-37b 基因转移由导致 Tregs 和 MDSC 并且调整 cytokine 生产在导致决策支持系统的大肠炎老鼠提高 MSC 的治疗学的功效。 | Wei-qiang WANG Kui DONG Lu ZHOU Guo-hui JIAO Cong-zhong ZHU Wen-wen LI Gang YU Wan-tong WU Song CHEN Zhi-na SUN Yu-ming WANG Wen-tian LIU Jie ZHANG Bang-mao WANG Xiao-ming FENG | 2015 | Acta Pharmacologica Sinica2015，36，11： | 13 |
| 4 | CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells显示文摘In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2,no specific and effective drugs for treating this disease have been reported until today.Angiotensin-converting enzyme 2(ACE2),a receptor of SARS-CoV-2,mediates the virus infection by binding to spike protein.Although ACE2 is expressed in the lung,kidney,and intestine,its expressing levels are rather low,especially in the lung.Considering the great infectivity of COVID-19,we speculate that SARS-CoV-2 may depend on other routes to facilitate its infection.Here,we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike protein.The loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody,Meplazumab,inhibits SARSCoV-2 amplification.Expression of human CD147 allows virus entry into non-susceptible BHK-21 cells,which can be neutralized by CD147 extracellular fragment.Viral loads are detectable in the lungs of human CD147(hCD147)mice infected with SARS-CoV-2,but not in those of virus-infected wild type mice.Interestingly,virions are observed in lymphocytes of lung tissue from a COVID-19 patient.Human T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dosedependent manner,which is specifically inhibited by Meplazumab.Furthermore,CD147 mediates virus entering host cells by endocytosis.Together,our study reveals a novel virus entry route,CD147-spike protein,which provides an important target for developing specific and effective drug against COVID-19. | Ke Wang Wei Chen Zheng Zhang Yongqiang Deng Jian-Qi Lian Peng Du Ding Wei Yang Zhang Xiu-Xuan Sun Li Gong Xu Yang Lei He Lei Zhang Zhiwei Yang Jie-Jie Geng Ruo Chen Hai Zhang Bin Wang Yu-Meng Zhu Gang Nan Jian-Li Jiang Ling Li Jiao Wu Peng Lin Wan Huang Liangzhi Xie Zhao-Hui Zheng Kui Zhang Jin-Lin Miao Hong-Yong Cui Min Huang Jun Zhang Ling Fu Xiang-Min Yang Zhongpeng Zhao Shihui Sun Hongjing Gu Zhe Wang Chun-Fu Wang Yacheng Lu Ying-Ying Liu Qing-Yi Wang Huijie Bian Ping Zhu Zhi-Nan Chen | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 9 |
| 5 | Electrochemical sensing of DNA immobilization and hybridization based on carbon nanotubes/nano zinc oxide/chitosan composite film显示文摘一个新奇电气化学的脱氧核糖核酸生物传感器基于氧化锌(ZnO ) ,为脱氧核糖核酸固定和提高的杂交察觉的 nanoparticles 和多围的碳 nanotubes (MWNT ) 被介绍。MWNTs/nano ZnO/chitosan 合成电影修改了玻璃化炭黑电极(MWNTs/ZnO/CHIT/GCE ) 被制作,脱氧核糖核酸探针在电极表面上被使不能调动。杂交事件被微分脉搏 voltammetry (DPV ) 作为指示物用亚甲蓝(MB ) 监视。传感器能有效地区别不同脱氧核糖核酸序列与有关在转基因的玉米轻拍基因,与 2.8 ×的检测极限 10 ? 目标顺序的 12 mol/L。 | Wei Zhang Tao Yang Da Ming Huang Kui Jiao | 2008 | Chinese Chemical Letters2008，19，5： | 8 |
| 6 | CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta,alpha,beta,and gamma显示文摘SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape,compromising the effectiveness of existing vaccines and neutralizing antibodies.An in-depth investigation on COVID-19 pathogenesis is urgently needed to develop a strategy against SARS-CoV-2 variants.Here,we identified CD147 as a universal receptor for SARS-CoV-2 and its variants.Meanwhile,Meplazeumab,a humanized anti-CD147 antibody,could block cellular entry of SARS-CoV-2 and its variants-alpha,beta,gamma,and delta,with inhibition rates of 68.7,75.7,52.1,52.1,and 62.3%at 60μg/ml,respectively.Furthermore,humanized CD147 transgenic mice were susceptible to SARS-CoV-2 and its two variants,alpha and beta.When infected,these mice developed exudative alveolar pneumonia,featured by immune responses involving alveoli-infiltrated macrophages,neutrophils,and lymphocytes and activation of IL-17 signaling pathway.Mechanistically,we proposed that severe COVID-19-related cytokine storm is induced by a'spike protein-CD147-CyPA signaling axis':Infection of SARS-CoV-2 through CD147 initiated the JAK-STAT pathway,which further induced expression of cyclophilin A(CyPA);CyPA reciprocally bound to CD147 and triggered MAPK pathway.Consequently,the MAPK pathway regulated the expression of cytokines and chemokines,which promoted the development of cytokine storm.Importantly,Meplazumab could effectively inhibit viral entry and inflammation caused by SARS-CoV-2 and its variants.Therefore,our findings provided a new perspective for severe COVID-19-related pathogenesis.Furthermore,the validated universal receptor for SARS-CoV-2 and its variants can be targeted for COVID-19 treatment. | Jiejie Geng Liang Chen Yufeng Yuan Ke Wang Youchun Wang Chuan Qin Guizhen Wu Ruo Chen Zheng Zhang Ding Wei Peng Du Jun Zhang Peng Lin Kui Zhang Yongqiang Deng Ke Xu Jiangning Liu Xiuxuan Sun Ting Guo Xu Yang Jiao Wu Jianli Jiang Ling Li Kun Zhang Zhe Wang Jing Zhang Qingguo Yan Hua Zhu Zhaohui Zheng Jinlin Miao Xianghui Fu Fengfan Yang Xiaochun Chen Hao Tang Yang Zhang Ying Shi Yumeng Zhu Zhuo Pei Fei Huo Xue Liang Yatao Wang Qingyi Wang Wen Xie Yirong Li Mingyan Shi Huijie Bian Ping Zhu Zhi-Nan Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 6 |
| 7 | Ultrahigh energy density battery-type asymmetric supercapacitors: NiMoO4 nanorod-decorated graphene and graphene/Fe2O3 quantum dots显示文摘 | Jiao Yang Wei Liu Hao Niu Kui Cheng Ke Ye Kai Zhu Guiling Wang Dianxue Cao Jun Yan | 2018 | Nano Research2018，11，9： | 5 |
| 8 | Direct electrocatalytic reduction of p-nitrophenol at room temperature ionic liquid modified electrode显示文摘p-nitrophenol (PNP ) 的直接电气化学的减小在离子的液体 N-butylpyridinium hexafluorophosphate (BPPF6 ) 修改了的房间温度上被调查碳浆糊电极(CILE ) 。cathodic 山峰潜力断然被转移,山峰水流与在传统的碳浆糊电极(用户终端设备) 上获得了那相比被增加。结果显示电极表面上的离子的液体 BPPF6 的存在显示出优秀催化能力到 PNP 的电气化学的减小。CILE 上的 PNP 的电气化学的行为被象扫描率那样的周期的 voltammetry 和条件调查,缓冲区 pH,底层集中被优化。电气化学的参数进一步与电子转移数字(n) 的结果被计算,费用转移系数(伪) 和表面集中(螕 T ) 作为 1.76, 0.37 和 2.47 脳 10 ? 9 mol/cm2,分别地为选择减少的山峰。结果显示 PNP 在 CILE 上显示出一个不可逆的控制吸附的电极过程。 | Wei Sun Mao Xia Yang Qiang Jiang Kui Jiao | 2008 | Chinese Chemical Letters2008，19，10： | 5 |
| 9 | Electrochemical Study of Lincomycin on Au-PtNPs/nanoPAN/ Chitosan Nanocomposite Membrane and Its Determination in Injections显示文摘The Au-Pt alloy nanoparticles(Au-PtNPs) were electrochemically deposited on the surface of polyaniline nanotube(nanoPAN) and chitosan(CS) modified glassy carbon electrode(GCE). The electrochemical behavior of lincomycin at Au-PtNPs/nanoPAN/CS modified GCE was investigated by cyclic voltammetry, linear sweep voltammetry and chronocoulometry. Cyclic voltammetric experiments show that lincomycin at the nanocomposite membrane modified electrode exhibited a pair of quasi-reversible redox peaks in pH=6.0 PBS. The membrane could accelerate the electron transfer of lincomycin on the electrode and significantly enhance the peak current. In a range of 3.0―100.0 mg/L, the reductive peak current of lincomycin at 0.42 V was linearly related to its concentration and the linear regression equation was ip,c=0.2703ρ–0.0042(ip,c: μA; ρ: mg/L; r=0.998, n=7) with a detection limit of 1.0 mg/L(S/N =3). Compared with other methods, this method exhibited many advantages such as high sensitivity, selectivity, wide linear range and low detection limit. The method was used to determine the content of lincomycin in injections commercially available with satisfactory results. Some electrochemical parameters involved in the redox reaction of lincomycin, such as parameter of kinetic nα, standard rate constant ks and the number of H+, were also calculated. | WANG Xue-liang YANG Tao JIAO Kui | 2010 | Chemical Research in Chinese Universities2010，26，3： | 3 |
| 10 | Al^(3+)/graphene composites for electrochemical detection of DNA cleavage显示文摘Herein, we present the electrochemical co-deposition of Al3+/graphene composites directly from an aqueous mixture containing graphene oxide (GO) and Al3+. The obtained Al3+/graphene composites with good electrochemical activity were regarded as an appropriate immobilization platform for double-stranded DNA (dsDNA). The nontoxic redox probe xanthurenic acid (XA) was successfully applied to recognize single-stranded DNA and dsDNA. We illustrated that the scission of dsDNA caused by GO combining with some metal ions could be detected by monitoring the electrochemical signals of XA. | DU Meng KONG QianQian YANG Tao JIAO Kui | 2013 | Science China Chemistry2013，56，9： | 3 |
| 11 | Development of catalytic combustion and CO_(2)capture and conversion technology显示文摘Changes are needed to improve the efficiency and lower the CO_(2)emissions of traditional coal-fired power generation,which is the main source of global CO_(2)emissions.The integrated gasification fuel cell(IGFC)process,which combines coal gasification and high-temperature fuel cells,was proposed in 2017 to improve the efficiency of coal-based power generation and reduce CO_(2)emissions.Supported by the National Key R&D Program of China,the IGFC for nearzero CO_(2)emissions program was enacted with the goal of achieving near-zero CO_(2)emissions based on(1)catalytic combustion of the flue gas from solid oxide fuel cell(SOFC)stacks and(2)CO_(2)conversion using solid oxide electrolysis cells(SOECs).In this work,we investigated a kW-level catalytic combustion burner and SOEC stack,evaluated the electrochemical performance of the SOEC stack in H2O electrolysis and H2O/CO_(2)co-electrolysis,and established a multiscale and multi-physical coupling simulation model of SOFCs and SOECs.The process developed in this work paves the way for the demonstration and deployment of IGFC technology in the future. | Zhibin Yang Ze Lei Ben Ge Xingyu Xiong Yiqian Jin Kui Jiao Fanglin Chen Suping Peng | 2021 | International Journal of Coal Science & Technology2021，8，3： | 3 |
| 12 | Direct numerical simulation of low Reynolds number turbulent air-water transport in fuel cell flow channel显示文摘With performance improvement of low-temperature fuel cell(FC), high reactant supply and water generation rates may induce air-water turbulence in the FC flow channel. In this research, an air-water turbulent direct numerical simulation(DNS) model is developed to simulate different droplet sizes,locations and interactions in the air-water transport processes comprehensively. It is found that a larger droplet breaks up more easily in turbulence, and a smaller droplet tends to keep lumped. The droplet at corner does not break up because it is away from channel center. The droplet interaction simulations show that the small droplets merge to form slugs, but still keep lumped in turbulence. It is suggested that two conditions need to be satisfied for droplet break up in FC flow channel, one is turbulent flow, and another is that the droplet needs to be large enough and occupy the center region of flow channel to suffer sufficient turbulence fluctuations. The DNS results illustrate some unique phenomena in turbulent flow, and show that the turbulence has significant effect on the air-water flow behavior in FC flow channel. | Zhiqiang Niu Renfang Wang Kui Jiao Qing Du Yan Yin | 2017 | Science Bulletin2017，62，1： | 3 |
| 13 | Analysis of Vitamin K_3 by a Fluorescent Spectroelectrochemistry Method显示文摘A simple and sensitive spectroelectrochemistry method for the determination of vitamin K 3 was developed by combining electrolysis and fluoremetry. This method was based on that vitamin K 3 was reduced at a glassy carbon electrode, and its product with characteristic fluorescence at 420 nm was determined with excitation wavelength at 309 nm. Under optimized electrochemical reaction conditions and fluorescent experiment parameters, the fluores-cence intensity was proportional to the concentration of vitamin K 3 in a range from 3.50×10 ?7 to 1.05×10 ?5 mol/L with a correlation coefficient of 0.9991, and detection limit was estimated to be 7.50×10 ?8 mol/L at a signal/noise ra-tio of 3. The relative standard deviation was less than 4.3%(n=5) and the recovery was in a range of 97%―105% for the determination of vitamin K 3 in pharmaceutical preparations. The result is satisfactory for the determination of vi-tamin K 3 as comparison to that from HPLC method. | ZHAO Chang-zhi LI Ying DU Hong XU Hua-jun JIAO Kui | 2010 | Chemical Research in Chinese Universities2010，26，5： | 3 |
| 14 | Controllable fabrication of Au micro/nanostructures on self-doped polyaniline nanofibers via electrochemical deposition and its application for DNA immobilization显示文摘Four electrochemical methods, cyclic voltammetric deposition, potentiostatic electrodeposition, multi-potential step electrodeposition and three-step electrodeposition, were used to fabricate Au micro/nanostructures on self-doped polyaniline nanofibers-coated glassy carbon electrodes (Au/nanoSPAN/GCEs). The Au micro/nanostructures deposited on the nanoSPAN-modified electrodes were shown by scanning electron microscopy to exhibit different morphologies, such as Au nanoparticle clusters, monodisperse nanoparticles and homogeneously dispersed flower-like microparticles, depending on the deposition method. This phenomenon demonstrates that control over the morphology of Au metal can be easily achieved by adjusting the electrodeposition method. The electrochemical behaviors of the Au/nanoSPAN/GCEs also varied with above four methods, which were characterized by cyclic voltammetry and electrochemical impedance spectroscopy. In comparison with Au nanoparticle clusters and monodisperse Au nanoparticles, homogeneously dispersed flower-like Au microparticles had the largest surface area and obviously enhanced electrochemical response towards the redox reactions of [Fe(CN)6]3–/4– on the modified electrode. DNA immobilization on the Au/nanoSPAN/GCEs was investigated by differential pulse voltammetry using [Fe(CN)6]3–/4– as an indicator. The efficiency of DNA immobilization was inherently related to their different Au micro/nanostructure morphologies. The Au/nano-SPAN/GCE fabricated by three-step electrodeposition showed the largest capacity for immobilization of single stranded DNA, which makes it a promising DNA biosensor. | WANG XinXing YANG Tao JIAO Kui | 2010 | Chinese Science Bulletin2010，55，36： | 3 |
| 15 | Multi-physics-resolved digital twin of proton exchange membrane fuel cells with a data-driven surrogate model显示文摘The development of multi-physics-resolved digital twins of proton exchange membrane fuel cells(PEMFCs)is sig-nificant for the advancement of this technology.Here,to solve this scientific issue,a surrogate modelling method that combines a state-of-the-art three-dimensional PEMFC physical model and data-driven model is proposed.The surrogate modelling prediction results demonstrate that the test-set relative root mean square errors(rRMSEs)of the multi-physics fields range from 3.88%to 24.80%and can mirror the multi-physics field distribution charac-teristics well.In summary,for multi-physics field prediction,the data-driven surrogate model has a comparable accuracy to the comprehensive 3D physical model;however,it considerably reduces the cost of computation and time and achieves the efficient multi-physics-resolved digital-twin.Two model-based designs based on the as-developed digital twin framework,i.e.the PEMFC healthy operation envelope and the PEMFC state map,are demonstrated.This study highlights the potential of combining data-driven approaches and comprehensive physical models to develop the digital twin of complex systems,such as PEMFCs. | Bowen Wang Guobin Zhang Huizhi Wang Jin Xuan Kui Jiao | 2020 | Energy and AI2020，1，1： | 3 |
| 16 | Thin-layered MoS_2/polyaniline nanocomposite for highly sensitive electrochemical detection of chloramphenicol显示文摘In this study, we synthesized molybdenum disulfide/polyaniline(MoS_2/PANI) nanocomposite via in situ polymerization of aniline in the presence of thin-layered MoS_2. The as-prepared MoS_2/PANI nanocomposite obtained an improved electrochemical performance due to the physisorption interaction between aromatic aniline and the basal plane of MoS_2. Furthermore, we constructed a new kind of electrochemical sensor based on MoS_2/PANI nanocomposite for the detection of chloramphenicol, which showed an excellent performance. The sensor has a high sensitivity and wide detection range from 1 x10^(-7)mol/L to 1x10^(-4)mol/L, with a low detection limit of 6.9 x 10^(-8)mol/L. | Huai-Yin Chen Jin Wang Le Meng Tao Yang Kui Jiao | 2016 | Chinese Chemical Letters2016，27，2： | 3 |
| 17 | Nano Au/TiO_2 hollow microsphere membranes for the improved sensitivity of detecting specific DNA sequences related to transgenes in transgenic plants显示文摘Gold nanoparticles (nano Au)/titanium dioxide (TiO2) hollow microsphere membranes were prepared on the carbon paste electrode (CPE) for enhancing the sensitivity of DNA hybridization detection. The immobilization of nano Au and TiO2 microsphere was investigated with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The hybridization events were monitored with EIS us-ing [Fe(CN)6]3-/4- as indicator. The sequence-specific DNA of the 35S promoter from cauliflower mosaic virus (CaMV35S) gene was detected with this DNA electrochemical sensor. The dynamic detection range was from 1.0×10-12 to 1.0×10-8 mol/L DNA and a detection limit of 2.3×10-13 mol/L could be ob-tained. The polymerase chain reaction (PCR) amplification of the terminator of nopaline synthase (NOS) gene from the real sample of a kind of transgenic soybean was also satisfactorily detected. | ZHANG YongChun, YANG Tao, ZHOU Na, ZHANG Wei & JIAO Kui College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China | 2008 | Science China Chemistry2008，51，11： | 2 |
| 18 | Elucidating the operating behavior of PEM fuel cell with nickel foam as cathode flow field显示文摘Metal foam material,which serves as an alternative replacement of the conventional flow distributor of proton exchange membrane(PEM)fuel cell,has been attracting much attention over last few decades.In this work,three-dimensional modeling work for PEM fuel cell containing metal foam as cathode flow distributor has been carried out.The fuel cell performance and operating characteristics of metal foam flow field and conventional parallel flow channel have been compared and discussed.The cell performance has been reasonably validated based on the corresponding experimental tests conducted in this study.The superior performance of PEM fuel cell with metal foam as cathode flow field benefits a lot from the uniform gas flow.The porous metal foam material provides more pathways for the water delivery at the interface of metal foam flow field and gas diffusion layer(GDL),accelerating water removal capability of cathode.Because of the significant oxygen transfer loss in diffusion limited parallel channel,the operation of PEM fuel cell with parallel channel is found to be more sensitive to cathode humidification and oxygen supply at inlet.Due to the more uniform and effective electron transport though the porous electrode,it is possible to use thinner GDL in metal foam PEM fuel cell.It is expected that this study could give a good baseline of operating behavior of PEM fuel cell with metal foam flow distributor. | HUO Sen SHI WeiYu WANG RenFang LU BingBing WANG Yang JIAO Kui HOU ZhongJun | 2021 | Science China(Technological Sciences)2021，64，5： | 2 |
| 19 | Two-Phase Flow in Porous Electrodes of Proton Exchange Membrane Fuel Cell显示文摘Water management in porous electrodes bears significance due to its strong potential in determining the performance of proton exchange membrane fuel cell.In terms of porous electrodes,internal water distribution and removal process have extensively attracted attention in both experimental and numerical studies.However,the structural difference among the catalyst layer(CL),microporous layer(MPL),and gas diffusion layer(GDL)leads to significant challenges in studying the two-phase flow behavior.Given the different porosities and pore scales of the CL,MPL,and GDL,the model scales in simulating each component are inconsistent.This review emphasizes the numerical simulation related to porous electrodes in the water transport process and evaluates the effectiveness and weakness of the conventional methods used during the investigation.The limitations of existing models include the following:(i)The reconstruction of geometric models is difficult to achieve when using the real characteristics of the components;(ii)the computational domain size is limited due to massive computational loads in three-dimensional(3 D)simulations;(iii)numerical associations among 3 D models are lacking because of the separate studies for each component;(iv)the effects of vapor condensation and heat transfer on the two-phase flow are disregarded;(v)compressive deformation during assembly and vibration in road conditions should be considered in two-phase flow studies given the real operating conditions.Therefore,this review is aimed at critical research gaps which need further investigation.Insightful potential research directions are also suggested for future improvements. | Daokuan Jiao Kui Jiao Qing Du | 2020 | Transactions of Tianjin University2020，26，3： | 2 |
| 20 | Three- dimensional non-isothermal modeling of carbon monox- ide poisoning in high temperature proton exchange mem- brane fuel cells with phosphoric acid doped polybenzimi- dazole membranes 显示文摘 | Jiao Kui Alaefour Ibrahim E Li Xianguo | 2011 | Fuel2011，90，2： | 1 |
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