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2篇 您的检索式:作者名="Kuipeng XU"
    题名 作者 年代 出处 被引量
1Construction of high-density genetic linkage map of Pyropia yezoensis(Bangiales,Rhodophyta)and identifi cation of red color trait QTLs in the thalli显示文摘Pyropia yezoensis is an important macroalga because of its extensive global distribution and economic importance.Color is an important trait in the thalli of P.yezoensis,it is also an effective marker to identify the hybridization in genetic breeding.In this study,a high-density genetic linkage map was constructed based on high-throughput single nucleotide polymorphism(SNP)markers,and used for analyzing the quantitative trait loci(QTLs)of red color trait in the thalli of P.yezoensis.The conchospore undergoes meiosis to develop into an ordered tetrad,and each cell has a haploid phenotype and can grow into a single individual.Based on this theory,F1 haploid population was used as the mapping population.The map included 531 SNP markers,394.57 cM long on average distance of 0.74 cM.Collinear analysis of the genetic linkage map and the physical map indicated that the coverage between the two maps was 79.42%.Furthermore,QTL mapping identified six QTLs for the chromosomal regions associated with the red color trait of the thalli.The value of phenotypic variance explained(PVE)by an individual QTL ranged from 4.71%-63.11%.And QTL qRed-1-1,with a PVE of 63.11%,was considered the major QTL.Thus,these data may provide a platform for gene and QTL fine mapping,and marker-assisted breeding in P.yezoensis in the future.Lu WANG Kuipeng XU Xianghai TANG Junhao WANG Fanna KONG Yunxiang MAO 2021Journal of Oceanology and Limnology2021,39,3:0
2Distribution, Function and Polymorphism Characteristics of Microsatellites in Pyropia yezoensis Transcriptome显示文摘The distribution, putative function and polymorphism characteristics of simple sequence repeats(SSRs) in P. yezoensis transcriptome were analyzed in this study. In total, 3076 SSRs were detected among 2681 unigenes. Trinucleotide types were dominant, constituting 91.87% of all the microsatellites. The most abundant SSR was CCG(71.03%) and the second abundant one was AGC(234, 7.61%). A total of 111(3.61%) dinucleotide types were found and the most abundant one was AC(51, 1.66%) which was followed by CG(34, 1.11%). SSRs identified showed a GC bases preference;GC bases constituted 89.73% of all the SSR bases. SSRs occurrence number decreased as repetitions increased. Annotation results exhibited that the majority of SSRs-containing unigenes have the functions of ‘metabolic process’,‘binding’ and ‘catalytic activity’ and existed as the forms of ‘cell’,‘cell component’ and ‘organelle’. The dominant amino acids that SSRs coded were Ala(28.59%), Arg(26.02%), Gly(14.35%) and Pro(13.65%). Amplification results showed that 3 out 23 SSRs from transcriptome(13.04%) and 8 out 77 SSRs from genome(10.39%) were polymorphic.LIU Yang PAN Xue XU Kuipeng MAO Yunxiang 2019Journal of Ocean University of China2019,18,3:0
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