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| 1 | Incidence of human papilloma virus in esophageal squamous cell carcinoma in patients from the Lublin region显示文摘AIM:To assess the prevalence of human papilloma virus(HPV) in esophageal squamous cell carcinoma(ESCC) in the south-eastern region of Poland.METHODS:The study population consisted of 56 ESCC patients and 35 controls.The controls were patients referred to our department due to other nonesophageal and non-oncological disorders with no gross or microscopic esophageal pathology as confirmed by endoscopy and histopathology.In the ESCC patients,samples were taken from normal mucosa(56 mucosa samples) and from the tumor(56 tumor samples).Tissue samples from the controls were taken from normal mucosa of the middle esophagus(35 control samples).Quantitative determination of DNA was carried out using a spectrophotometric method.Genomic DNA was isolated using the QIAamp DNA Midi Kit.HPV infection was identified following PCR amplification of the HPV gene sequence,using primers MY09 and MY11 complementary to the genome sequence of at least 33 types of HPV.The sequencing results were computationally analyzed using the basic local alignment search tool database.RESULTS:In tumor samples,HPV DNA was identified in 28 of 56 patients(50%).High risk HPV phenotypes(16 or/and 18) were found in 5 of 56 patients(8.9%),low risk in 19 of 56 patients(33.9%) and other types of HPV(37,81,97,CP6108) in 4 of 56 patients(7.1%).In mucosa samples,HPV DNA was isolated in 21 of 56 patients(37.5%).High risk HPV DNA was confirmed in 3 of 56 patients(5.3%),low risk HPV DNA in 12 of 56 patients(21.4%),and other types of HPV in 6 of 56 patients(10.7%).In control samples,HPV DNA was identified in 4 of 35 patients(11.4%) with no high risk HPV.The occurrence of HPV in ESCC patients was significantly higher than in the controls [28 of 56(50%) vs 4 of 35(11.4%),P < 0.001].In esophageal cancer patients,both in tumor and mucosa samples,the predominant HPV phenotypes were low risk HPV,isolated 4 times more frequently than high risk phenotypes [19 of 56(33.9%) vs 5 of 56(8.9%),P < 0.001].A higher prevalence of HPV was identified in female patients(71.4% vs 46.9%).Accordingly,the high risk phenotypes were isolated more frequently in female patients and this difference reached statistical significance [3 of 7(42.9%) vs 2 of 49(4.1%),P < 0.05].Of the pathological characteristics,only an infiltrative pattern of macroscopic tumor type significantly correlated with the presence of HPV DNA in ESCC samples [20 of 27(74.1%) vs 8 of 29(27.6%) for ulcerative or protruding macroscopic type,P < 0.05].The occurrence of total HPV DNA and both HPV high or low risk phenotypes did not significantly differ with regard to particular grades of cellular differentiation,phases in depth of tumor infiltration,grades of nodal involvement and stages of tumor progression.CONCLUSION:Low risk HPV phenotypes could be one of the co-activators or/and co-carcinogens in complex,progressive,multifactorial and multistep esophageal carcinogenesis. | Andrzej Dabrowski Wojciech Kwasniewski Tomasz Skoczylas Wiesawa Bednarek Dorota Kuzma Anna Gozdzicka-Józefiak | 2012 | World Journal of Gastroenterology2012，18，40： | 55 |
| 2 | Shoot-Specific Down-Regulation of Protein Farnesyltransferase (α-Subunit) for Yield Protection against Drought in Canola显示文摘Canola (Brassica 马来麝 L.) 是在世界和它的种子收益上的最重要的 oilseed 庄稼之一,质量被干旱应力显著地影响。作为对水赤字的天生、适应的回答,陆地植物由植物激素 abscisic 酸(骆驼毛的织物) 的快速的生合成避免潜在的损坏,它触发有气孔的闭合减少 transpirational 水损失。调停骆驼毛的织物的有气孔的反应是一个剂量依赖者过程;因此,为完成干旱回避的一条遗传工程途径能是敏化警卫房间的应答到这荷尔蒙。最近的基因研究作为在警卫房间控制骆驼毛的织物敏感的一个关键否定管理者指出了蛋白质 farnesyltransferase。我们以前证明了编码 farnesyltransferase (ERA1 ) 的 Arabidopsis 子单元的基因的下面规定提高植物的敏感到骆驼毛的织物和干旱忍耐。尽管 farnesyltransferase (AtFTA ) 的子单元也在骆驼毛的织物察觉到被含有,使用如此的一个基因目标在庄稼植物改进干旱忍耐的有效性没被验证。这里,我们报导 Arabidopsis hydroxypyruvate reductase (AtHPR1 ) 的倡导者的鉴定和描述,它在射击并且不在象根那样的非光合的纸巾明确地表示。AtHPR1 的倡导者区域包含元素和我们证实了的很好描绘的脱水应答的行动 cis 的核心主题那 AtHPR1 表情由干旱应力是可诱导的。在用驾驶 RNAi 构造的 AtHPR1 倡导者的 canola 的 FTA 的有条件、特定的下面规定在这块地里对干旱应力导致了收益保护。用这分子的策略,我们在在这重要庄稼种设计干旱忍耐取得了重要进步。 | Yang Wang Michelle Beaith Maryse Chalifoux Jifeng Ying Tina Uchacz Carlene Sarvas Rebecca Griffiths Monika Kuzma Jiangxin Wan Yafan Huang | 2009 | Molecular Plant2009，2，1： | 7 |
| 3 | 提高薄膜太阳能电池的效率显示文摘降低硅太阳能电池成本的方法之一是尽量减少高质量硅材料的使用量,如薄膜太阳能电池。不过这种太阳能电池的效率只达到了约11-12%。研究人员们正在寻求提升其效率的方法。 | Izabela Kuzma Guy Beaucarne Jef Poortmans | 2008 | 集成电路应用2008，，9： | 3 |
| 4 | Bilateral apical vs nonapical stapling resection during lung volume reduction surgery显示文摘 | Tranalin JM Furukawa S kuzma AM | 1998 | Chest1998，14，4： | 1 |
| 5 | Multidisciplinary care of the patient with chronic obstructive pulmonary disease 显示文摘 | Kuzma AM Meli Y Meldrum C | 2008 | Proc Am Thorac Soc2008，5，4： | 1 |
| 6 | African Americans and Men with Severe COPD Have a High Prevalence of Osteoporosis显示文摘 | Lihua Li Kathleen J. Brennan John P. Gaughan David E. Ciccolella Anne Marie Kuzma Gerard J. Criner | 2008 | Copd: Journal of Chronic Obstructive Pulmonary Disease2008，，5： | 1 |
| 7 | Accessibility Design Issues with UK e-Government Sites显示文摘 | Kuzma J M | 2010 | Government Information Quarterly2010，27，2： | 1 |
| 8 | Molecular tailoring of famesy- lation for plant drought tolerance and yield protection显示文摘 | Wang Y Ying J F Kuzma M | 2005 | The Plant Journal2005，43，： | 1 |
| 9 | Distal radius fractures arthroscopic-assisted fixation显示文摘 | Kuzma GR Kuzma KR | 2010 | Oper Tech Sports Med2010，18，3： | 1 |
| 10 | Multidisciplinary care of the patient with chronic obstructive pulmonary disease显示文摘 | Kuzma AM Meli Y Meldrum C | | 0，，： | 1 |
| 11 | Syncope in children and adolescents 显示文摘 | Rudzinski A Oko-Lagan J Kuzma J | 2007 | Przegl Lek2007，64，3： | 1 |
| 12 | Diterpenoids and triterpenoids in hairy roots of Salvia sclarea 显示文摘 | KUZMA SKRZYPE K Z WYSOKINSKA H | 2006 | Plant Cell Tissue andOrgan Culture2006，84，10： | 1 |
| 13 | Evaluation, develap- ment,and implementation of potentially better practices in neonatal in- tensive care nutrition 显示文摘 | Kuzma - O'Reilly B Duenas ML Greecher C | 2003 | Pediatrics2003，111，4： | 1 |
| 14 | Self-report of Physical Activity and Patterns of Mortality in Seventh-Day Adventist Men显示文摘 | Lindsted K D Tonstad S Kuzma J W | 1991 | J Clin Epidemiol1991，44，45： | 1 |
| 15 | Abscisic acid signaling through cyclic ADP - fibose in plants 显示文摘 | WU Y KUZMA J MARECHAL E | 1997 | Science1997，278，： | 1 |
| 16 | Growth hormone deficiency,its influence on bone mineral density and risk of osteoporotic fractures显示文摘 | Kuzma M Payer J | | 0，，05： | 1 |
| 17 | Abscisic acid signaling through cyclic ADP-ribo in plants显示文摘 | WU Y KUZMA J MARECHAL E | 1997 | Science1997，278，： | 1 |
| 18 | Growth hormone deficiency,its influence on bone mineral density and risk of osteoporotic fractures 显示文摘 | KUZMA M PAYER J | 2010 | Cas Lek Cesk2010，149，5： | 1 |
| 19 | 低脂和全脂乳制品对代谢综合征患者空腹血脂和血压的影响:一项随机对照试验的探索性终点显示文摘传统膳食指南推荐低脂乳制品,因为乳制品高饱和脂肪含量可加重心血管病。然而,新出现的证据表明,当摄入含复合营养的食物时,乳制品脂肪或许对心血管病危险因素无不良影响。本研究旨在比较限制乳制品膳食和富含低脂或全脂乳制品膳食对心血管病危险因素的影响。方法:在本项随机对照试验当中,72例患有代谢综合征的参与者完成4周的导入期,限制乳制品摄入(脱脂牛奶摄入量≤3份/周)。 | 袁源(摘译) 郑武洪(审校) Schmidt KA Cromer G Burhans MS Kuzma JN Hagman DK Fernando I Murray M Utzschneider KM Holte S Kraft J Kratz M | 2021 | 中华高血压杂志2021，29，10： | 1 |
| 20 | Leaf isoprene emission rate is dependent on leaf development and the level of isoprene sy ntheses显示文摘 | Kuzma J Fall R | 1993 | Plant Physiology1993，101，： | 1 |
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