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| 1 | 轻度缺乏磷的日粮中随着钙和有效磷比值增加对保育猪生长性能和骨骼发育的不利影响显示文摘文章旨在评估在含植酸酶日粮中(250 FTU/kg)增加钙(Ca)和有效磷(aP)比例对保育猪生长性能和骨骼矿物质含量(骨密度,BMC)的影响。试验将480头保育猪(体重BW=5.7±0.6 kg)随机分配为7个处理,Ca∶aP递增分别为:1.25∶1、1.50∶1、1.75∶1、2.00∶1、2.25∶1、2.50∶1、2.75∶1,每个处理7个重复(其中6个重复饲喂2.75∶1日粮),每个重复10头猪。从试验正式开始前7 d至第0天,所有仔猪饲喂相同日粮。试验期分为两个阶段(阶段1和阶段2),分别为1—14 d和15—28 d,试验猪只在试验期饲喂处理日粮。阶段1和阶段2日粮配方中有效磷比例分别为0.33%和0.27%(大约为需要量的90%)。测定体重(BW)、平均日增重(ADG)、平均日采食量(ADFI)和体重与饲料比(G∶F)。试验第28天使用双X射线骨密度仪测定每头猪肱骨的BMC。数据采用PROC MIXED(SAS 9.3)线性混合模型分析。利用正交多项式对比计算Ca∶aP递增的线性和二次效应。整个28 d试验期,Ca∶aP递增导致ADG(353、338、328、304、317、291和280 g/d;P<0.01)、ADFI(539、528、528、500、533、512和489 g/d;P<0.05)和G∶F(0.68、0.66、0.64、0.62、0.61、0.59和0.58;P<0.01)呈线性递减。Ca∶aP递增也会导致第14天和第28天BW降低(P<0.01)。肱骨的BMC随着Ca∶aP递增而减少(6.2、6.3、5.7、5.9、5.5、5.6和5.3 g;P<0.01)。下面是Ca∶aP对各指标的回归分析影响,其中X是Ca∶aP:ADG[g/d]=339-36x,r^2=0.81;G:F=0.61-0.03x,r^2=0.72;BMC[g]=6.4-0.27x,r^2=0.43。所有结果表明,任何高于这次试验中所使用的最低Ca∶aP的钙水平日粮均会损害动物生长性能和骨骼发育。有必要进一步研究日粮中更低水平钙含量。 | 何颖(编译) Spenser L.Becker Stacie A.Gould Amy L.Petry Leah M.Kellesvig John.F.Patience | 2020 | 猪业科学2020，37，12： | 3 |
| 2 | Lgr5, an intestinal stem cell marker, is abnormally expressed in Barrett’s esophagus and esophageal adenocarcinoma显示文摘 | L.Becker Q.Huang H.Mashimo | 2010 | Diseases of the Esophagus2010，，2： | 1 |
| 3 | Procalcitonin in sepsis and systemic inflammation: a harmful biomarker and a therapeutic target显示文摘 | Kenneth L.Becker RichardSnider Eric S.Nylen | 2010 | British Journal of Pharmacology2010，，2： | 1 |
| 4 | Lgr5, an intestinal stem cell marker, is abnormally expressed in Barrett’s esophagus and esophageal adenocarcinoma显示文摘 | L.Becker Q.Huang H.Mashimo | 2010 | Diseases of the Esophagus2010，，2： | 1 |
| 5 | Procalcitonin in sepsis and systemic inflammation: a harmful biomarker and a therapeutic target显示文摘 | Kenneth L.Becker RichardSnider Eric S.Nylen | 2010 | British Journal of Pharmacology2010，，2： | 1 |
| 6 | Procalcitonin in sepsis and systemic inflammation: a harmful biomarker and a therapeutic target显示文摘 | Kenneth L.Becker RichardSnider Eric S.Nylen | 2010 | British Journal of Pharmacology2010，，2： | 1 |
| 7 | Lgr5, an intestinal stem cell marker, is abnormally expressed in Barrett’s esophagus and esophageal adenocarcinoma显示文摘 | L.Becker Q.Huang H.Mashimo | 2010 | Diseases of the Esophagus2010，，2： | 1 |
| 8 | Lgr5, an intestinal stem cell marker, is abnormally expressed in Barrett’s esophagus and esophageal adenocarcinoma显示文摘 | L.Becker Q.Huang H.Mashimo | 2010 | Diseases of the Esophagus2010，，2： | 1 |
| 9 | Systematic Cross-biospecimen Evaluation of DNA Extraction Kits for Long-and Short-read Multi-metagenomic Sequencing Studies显示文摘High-quality DNA extraction is a crucial step in metagenomic studies.Bias by different isolation kits impairs the comparison across datasets.A trending topic is,however,the analysis of multiple metagenomes from the same patients to draw a holistic picture of microbiota associated with diseases.We thus collected bile,stool,saliva,plaque,sputum,and conjunctival swab samples and performed DNA extraction with three commercial kits.For each combination of the specimen type and DNA extraction kit,20-gigabase(Gb)metagenomic data were generated using short-read sequencing.While profiles of the specimen types showed close proximity to each other,we observed notable differences in the alpha diversity and composition of the microbiota depending on the DNA extraction kits.No kit outperformed all selected kits on every specimen.We reached consistently good results using the Qiagen QiAamp DNA Microbiome Kit.Depending on the specimen,our data indicate that over 10 Gb of sequencing data are required to achieve sufficient resolution,but DNA-based identification is superior to identification by mass spectrometry.Finally,longread nanopore sequencing confirmed the results(correlation coefficient>0.98).Our results thus suggest using a strategy with only one kit for studies aiming for a direct comparison of multiple microbiotas from the same patients. | Jacqueline Rehner Georges Pierre Schmartz Laura Groeger Jan Dastbaz Nicole Ludwig Matthias Hannig Stefan Rupf Berthold Seitz Elias Flockerzi Tim Berger Matthias Christian Reichert Marcin Krawczyk Eckart Meese Christian Herr Robert Bals Soren L.Becker Andreas Keller Rolf Muller The IMAGINE Consortium | 2022 | Genomics, Proteomics & Bioinformatics2022，20，2： | 0 |
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