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| 1 | Overexpression of micro RNA_(40)8 enhances photosynthesis, growth, and seed yield in diverse plants显示文摘The ability of a plant to produce grain, fruit, or forage depends ultimately on photosynthesis. There have been few attempts, however, to study micro RNAs, which are a class of endogenous small RNAs post-transcriptionally programming gene expression, in relation to photosynthetic traits. We focused on miR_(40)8, one of the most conserved plant miRNAs, and overexpressed it in parallel in Arabidopsis, tobacco, and rice. The transgenic plants all exhibited increased copper content in the chloroplast, elevated abundance of plastocyanin,and an induction of photosynthetic genes. By means of gas exchange and optical spectroscopy analyses, we showed that higher expression of miR_(40)8 leads to enhanced photosynthesis through improving efficiency of irradiation utilization and the capacity for carbon dioxide fixation. Consequently, miR_(40)8 hyper-accumulating plants exhibited higher rate of vegetative growth. An enlargement of seed size was also observed in all three species overproducing miR_(40)8. Moreover, we conducted a 2-year-two-location field trial and observed miR_(40)8 overexpression in rice significantly increased yield, which was primarily attributed to an elevation in grain weight. Taken together, these results demonstrate that miR_(40)8 is a positive regulator of photosynthesis and that its genetic engineering is a promising route for enhancing photosynthetic performance and yield in diverse plants. | Jiawei Pan Dahui Huang Zhonglong Guo Zheng Kuang He Zhang Xinyu Xie Zengfeng Ma Shaopei Gao Manuel T.Lerdau Chengcai Chu Lei Li | 2018 | Journal of Integrative Plant Biology2018，60，4： | 8 |
| 2 | Single Nucleotide Polymorphism of FSHβ Gene Associated with Reproductive Traits in Japanese Flounder (Paralichthys olivaceus)显示文摘Follicle stimulating hormone β (FSHβ) of Japanese flounder (Paralichthys olivaceus) plays a key role in the regulation of gonadal development.This study aimed to investigate molecular genetic characteristics of the FSHβ gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of FSHβ on reproductive traits in Japanese flounder.We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the FSHβ gene in 60 individuals.We identified only an SNP (T/C) in the coding region of exon3 of FSHβ.The SNP (T/C) did not lead to amino acid changes at the position 340 bp of FSHβ gene.Statistical analysis showed that the SNP was significantly associated with testosterone (T) level and gonadosomatic index (GSI) (P<0.05).Individuals with genotype TC of the SNP had significantly higher serum T levels and GSI (P<0.05) than that of genotype CC.Therefore, FSHβ gene could be a useful molecular marker in selection for prominent reproductive trait in Japanese Flounder. | HE Feng WEN Haishen YU Dahui LI Jifang SHI Bao CHEN Caifang ZHANG Jiaren JIN Guoxiong CHEN Xiaoyan Shi Dan Yang Yanping | 2010 | Journal of Ocean University of China2010，9，4： | 4 |
| 3 | Degradation of furan tebufenozide in laboratory and field trials显示文摘Furan tebufenozide is a newly developed insect growth regulator and has been applied as a pesticide in agriculture in China.Its degradation under both laboratory and field conditions was investigated,and the degradation kinetics was fitted by simple first order kinetics (SFO) model and first order double exponential (FOD) model.Laboratory studies were conducted with or without light in five simulated media (sterilized deionized water,river water,soil solution,sterilized soil and natural soil).No dissipations of furan tebufenozide were observed in sterilized aqueous and soil media under light prevented conditions,whereas degradation occurred under all the other conditions in the laboratory.Derived from SFO and FOD models,DT50 in the dark and light laboratory conditions was in the range of 39.7-82.5 and 1.1-8.0 days,respectively.These results indicated that microbes and light were the main factors for the degradation of the pesticide in the laboratory.During field trials,derived from the SFO model,DT50 and DT90 were 30.3 and 100.5 days,while derived from the FOD model,DT50 and DT90 were 28.9 and 274.9 days,respectively.Compared with laboratory experiments,field trials were influenced by multiple factors.Therefore,the SFO model could not fit experimental data as well as the FOD model did in field trials. | GUO Cong,LI DaHui,CHEN JinHui,GUO BaoYuan,WANG HuiLi & LI JianZhong Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China | 2010 | Science China Chemistry2010，53，8： | 3 |
| 4 | Identification of Major Locus Bph35 Resistance to Brown Planthopper in Rice显示文摘An introgression line RBPH660,derived from wild rice Oryza rufipogon,showed stable resistance to brown planthopper(BPH).Segregation analysis indicated BPH resistance of RBPH660 was controlled by multiple genes/QTLs.By using the bulked segregant analysis(BSA)-seq method,two genomic regions harboring QTLs resistance to BPH were identified from 1.20 to 16.70 Mb on chromosome 4 and from 10.20 to 12.60 Mb on chromosome 9 in RBPH660,respectively.A major resistance locus,designated as Bph35 accounting for 51.27%of the phenotypic variation with a LOD score of 42.51,was mapped to the candidate region of chromosome 4 between In Del(insertion-deletion)markers PSM16 and R4 M13.For fine mapping of Bph35,one simple sequence repeat and three newly developed In Del markers were used to screen the recombinants.Finally,the Bph35 locus was delimited in the region from 6.28 to 6.93 Mb and there were 18 predicted protein-encoding genes with a total of 114 non-synonymous single nucleotide polymorphism(SNP)variant sites between the resistant and susceptible parents.Out of these genes,Os04 g0193950,encoding a putative NB-ARC(nucleotidebinding adaptor shared by APAF-1,R proteins and CED-4)and LRR(leucine-rich repeat)domain protein with nine non-synonymous SNP substitutions in its coding sequence regions,might be the candidate gene for Bph35.These findings would facilitate the map-based cloning of the Bph35 gene and development of resistant varieties against BPH in rice. | ZHANG Yuexiong QIN Gang MA Qianqian WEI Minyi YANG Xinghai MA Zengfeng LIANG Haifu LIU Chi LI Zhenjing LIU Fang HUANG Dahui LI Rongbai | 2020 | Rice science2020，27，3： | 3 |
| 5 | An Empirical Assessment and Application of SERVQUAL in Mainland China's Mobile Communications Industry显示文摘 | Fujun Lai Joe Hutchinson Dahui Li | 2007 | International Journal of Quality & Reliability Management2007，24，3： | 1 |
| 6 | An empirical investigation of web Site use using a commitment-based model显示文摘 | Li Dahui Browne G J Chau P Y K | 2006 | Decision Sciences2006，37，3： | 1 |
| 7 | An empirical investigation of web site use using a commitment-based model显示文摘 | Li Dahui Browne G J Chau P Y K | 2006 | Decision Sciences2006，37，3： | 1 |
| 8 | Growing impacts of low-flow events on vegetation dynamics in hydrologically connected wetlands downstream Yangtze River Basin after the operation of the Three Gorges Dam显示文摘Wetland vegetation is intimately related to floodplain inundations,which can be seriously affected by dam operation.Poyang Lake is the largest floodplain wetland in China and naturally connected with the Yangtze River and the Three Gorges Dam(TGD)upstream.To understand the potential impacts of TGD on Poyang Lake wetlands,we collected remote sensing imagery acquired during dry season from 1987 to 2020 and extracted vegetation coverage data in the Ganjiang Northern-branch Delta(GND)and the Ganjiang Southern-branch Delta(GSD),using the Object-oriented Artificial Neural Network Regression.Principal components analysis,correlation analysis,and the random forest model were used to explore the interactions between vegetation extent in the two deltas and 33 hydrological variables regarding magnitude,duration,timing,and variation.The implementation of the TGD advanced and extended the low-flow periods in Poyang Lake.Vegetation coverage in the GND and GSD increased at the rates of 0.39 and 0.22 km2/year,respectively.The reservoir storage at the end of September accelerated the runoff recession in the GND and the GSD,making low-flow events more influential for vegetation dynamics and shortening the response time of vegetation to the water regime.This study provides an important reference for evaluating the impacts of dam engineering on downstream wetlands. | WANG Dandan LIU Ying ZHENG Lilin LI Dahui | 2023 | Journal of Geographical Sciences2023，33，4： | 1 |
| 9 | Reputation distribution and consumer-to-consumer online auction market structure:an exploratory study显示文摘 | Zhangxi Lin Dahui Li Balaji Janamanchi Wayne Huang | 2006 | Decision Support Systems2006，，41： | 1 |
| 10 | True reason for Zipf's law in language显示文摘 | Wang Dahui Li Menghui Di Zengru | 2005 | Physica A2005，358，234： | 1 |
| 11 | Task Design, Motivation, and Participation in Crowdsourcing Contests显示文摘 | Haichao Zheng Dahui Li Wenhua Hou | 2011 | International Journal of Electronic Com- merce2011，15，15： | 1 |
| 12 | Why Do Intemet Users Stick with a Specific Web Site?A Relationship Per- spective 显示文摘 | Li Dahui Browne G J Wetherbe J C | 2006 | Intemational Journal of Elec- tronic Commerce2006，10，4： | 1 |
| 13 | The role of multidimensional social capital in crowdfunding:A comparative study in China and US 显示文摘 | ZHENG Haichao LI Dahui WU Jing | 2014 | Information & Management2014，，51： | 1 |
| 14 | The role of multidimensional social capital in crowdfunding: A comparative study in China and US显示文摘 | Haichao Zheng Dahui Li Jing Wu Yun Xu | 2014 | Information & Management2014，，4： | 1 |
| 15 | Evolving model of weighted Networks Inspired by Scientific Collaboration Networks显示文摘 | Menghui Li Jinshan Wu Dahui Wang Tao Zhou Zengru Di Ying Fan | 2007 | Physica A2007，275，1： | 1 |
| 16 | E-Business assimilation in Chi- na's international trade firms: the technology-organization-envi- ronment framework 显示文摘 | Li Dahui Lai Fujun Wang Jian | 2010 | Journal of Global Information Manage- ment2010，18，1： | 1 |
| 17 | Task Design, Motivation, and Participation in Crowdsourcing Contests 显示文摘 | I-Iaichao Zheng Dahui Li and Wenhua Hou | 2011 | International Journal of Electronic Commerce2011，15，4： | 1 |
| 18 | Evolving Model of Weighted Networks Inspired by Scientific Collaboration Networks显示文摘 | Li Menghui Wu Jinshan Wang Dahui | 2005 | arXiv:cond-mat/05016552005，，05： | 1 |
| 19 | Online Consumer-to-consumer Market in China - A comparative Study of Taobao and eBay 显示文摘 | Dahui Li Jun Li Zhangxi Lin | 2008 | Electronic Commerce Research and Applications2008，4，7： | 1 |
| 20 | Why Do Interact Users Stick with A Specific Website? A Relationship Perspective 显示文摘 | Li Dahui Browne G J Wetherbe J C | 2006 | International Journal of Electronic Commerce2006，10，4： | 1 |
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