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| 1 | Discovery of biclonal origin and a novel oncogene SLC12A5 in colon cancer by single-cell sequencing显示文摘单个房间的定序是为描出的一个强大的工具为个性化的癌症管理的同种细胞的关系和识别关键司机基因。这里,我们执行了结肠癌的一个盒子的单个房间的定序的分析。人口遗传分析在肿瘤房间人口识别了二独立克隆。主要肿瘤克隆作为早 oncogenic 事件怀有 APC 和 TP53 变化,而次要的克隆包含了优势的 CDC27 和 PABPC1 变化。在这二克隆的次要的克隆支持的 APC 和 TP53 变化的缺席从二细胞的起源被导出。体的变化等位基因频率系列的检查另外 21 个整个织物的定序 exome 的盒子在结肠癌揭示了同种细胞的起源的异质。下次,我们识别了在单个房间的水平显示出变化的高频率,但是在人口水平展出了低流行的变异的基因 SLC12A5。变异的 SLC12A5 的功能的描述在结肠癌揭示了它的潜在的 oncogenic 效果。我们的学习在单个房间的水平支持提供第一条 exome 宽的证据那结肠癌能具有 biclonal 起源,并且建议在一个队的低流行的变化可以也在单个水平起重要 protumorigenic 作用。 | Chang Yu Jun Yu Xlaotlan Yao Wllham KK Wu Youyong Lu Senwel Tang Xiangchun Li Li Bao Xiaoxing Li Yong Hou Renhua Wu Min Jian Ruoyan Chen Fan Zhang Lixia Xu Fan Fan Jun He Qiaoyi Liang Hongyi Wang Xueda Hu Minghui He Xiang Zhang Hancheng Zheng Qibin Li Hanjie Wu Yan Chen Xu Yang Shida Zhu Xun Xu Huanming Yang Jian Wang Xiuqing Zhang Joseph JY Sung Yingrui Li Jun Wang | 2014 | Cell Research2014，，6： | 16 |
| 2 | Scutellarin protects oxygen/glucose-deprived astrocytes and reduces focal cerebral ischemic injury显示文摘Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxidative effects and has been used to treat cardiovascular and cerebrovascular diseases in China. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2) was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity. Cultures of primary astrocytes isolated from the cerebral cortex of neonatal Sprague-Dawley rats were pretreated with 2, 10 or 50 μM scutellarin for 30 minutes. The astrocytes were then subjected to oxygen/glucose deprivation by incubation for 2 hours in glucose-free Dulbecco's modified Eagle's medium in a 95% N2/5% CO_2 incubator, followed by simulated reperfusion for 22 hours. Cell viability was assessed by cell counting kit-8 assay. Expression levels of NOX2, connexin 43 and caspase-3 were assessed by western blot assay. Reactive oxygen species were measured spectrophotometrically. Pretreatment with 10 or 50 μM scutellarin substantially increased viability, reduced the expression of NOX2 and caspase-3, increased the expression of connexin 43, and diminished the levels of reactive oxygen species in astrocytes subjected to ischemia-reperfusion. We also assessed the effects of scutellarin in vivo in the rat transient middle cerebral artery occlusion model of cerebral ischemia-reperfusion injury. Rats were given intraperitoneal injection of 100 mg/kg scutellarin 2 hours before surgery. The Bederson scale was used to assess neurological deficit, and 2,3,5-triphenyltetrazolium chloride staining was used to measure infarct size. Western blot assay was used to assess expression of NOX2 and connexin 43 in brain tissue. Enzyme-linked immunosorbent assay was used to detect 8-hydroxydeoxyguanosine(8-OHd G), 4-hydroxy-2-nonenal(4-HNE) and 3-nitrotyrosin(3-NT) in brain tissue. Immunofluorescence double staining was used to determine the co-expression of caspase-3 and Neu N. Pretreatment with scutellarin improved the neurological function of rats with focal cerebral ischemia, reduced infarct size, diminished the expression of NOX2, reduced levels of 8-OHd G, 4-HNE and 3-NT, and reduced the number of cells co-expressing caspase-3 and Neu N in the injured brain tissue. Furthermore, we examined the effect of the NOX2 inhibitor apocynin. Apocynin substantially increased connexin 43 expression in vivo and in vitro. Collectively, our findings suggest that scutellarin protects against ischemic injury in vitro and in vivo by downregulating NOX2, upregulating connexin 43, decreasing oxidative damage, and reducing apoptotic cell death. | Jing-Bo Sun Yan Li Ye-Feng Cai Yan Huang Shu Liu Patrick KK Yeung Min-Zhen Deng Guang-Shun Sun Prince LM Zilundu Qian-Sheng Hu Rui-Xin An Li-Hua Zhou Li-Xin Wang Xiao Cheng | 2018 | Neural Regeneration Research2018，13，8： | 16 |
| 3 | Bis(7)-tacrine attenuates beta amyloid-induced neuronal apoptosis by regulating L-type calcium channels显示文摘 | Fu H Li W Lao Y Luo J Lee NT Kan KK Tsang HW Tsim KW Pang Y Li Z Chang DC Li M Han Y | 2006 | 中国生物学文摘2006，20，10： | 10 |
| 4 | Primary biliary cirrhosis-associated hepatocellular carcinoma in Chinese patients:Incidence and risk factors显示文摘AIM: To investigate the incidence, characteristics, and risk factors for hepatocellular carcinoma(HCC) in Chinese patients with primary biliary cirrhosis(PBC).METHODS: We reviewed the data of 52 PB Cassociated HCC patients treated at Beijing 302 Hospital from January 2002 to December 2013 and analyzed its incidence and characteristics between the two genders. The risk factors for PBC-associated HCC were analyzed via a case-control study comprising 20 PBC patients with HCC and 77 matched controls without HCC. The matched factors included gender, age, follow-up period and Child-Pugh scores. Conditional logistic regression was used to evaluate the odds ratios of potential risk factors for HCC development. A P < 0.05 was considered statistically significant. RESULTS: The incidence of HCC in Chinese PBC patients was 4.13%(52/1255) and was significantly higher in the males(9.52%) than in the females(3.31%). Among the 52 PBC patients with HCC, 55.76%(29/52) were diagnosed with HCC and PBC simultaneously, and 5.76%(3/52) were diagnosed with HCC before PBC. The males with PBC-associated HCCwere more likely than the females to have undergone blood transfusion(18.75% vs 8.33%, P = 0.043), consumed alcohol(31.25% vs 8.33%, P = 0.010), smoked(31.25% vs 8.33%, P = 0.010), had a family history of malignancy(25% vs 5.56%, P = 0.012), and had serious liver inflammation, as indicated by the elevated levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and γ-glutamyl transpeptidase(P < 0.05). Conditional logistic regression analysis revealed that body mass index(BMI) ≥ 25 [adjusted odds ratio(AOR) = 1.116, 95%CI: 1.002-1.244, P = 0.045] and history of alcohol intake(AOR = 10.294, 95%CI: 1.108-95.680, P = 0.040) were significantly associated with increased odds of HCC development in PBC patients. CONCLUSION: HCC is not rare in Chinese PBC patients. Risk factors for PBC-associated HCC include BMI ≥ 25 and a history of alcohol intake. In addition to regular monitoring, PBC patients may benefit from abstinence from alcohol and body weight control. | Xue-Xiu Zhang Li-Feng Wang Lei Jin Yuan-Yuan Li Shu-Li Hao Yan-Chao Shi Qing-Lei Zeng Zhi-Wei Li Zheng Zhang George KK Lau Fu-Sheng Wang | 2015 | World Journal of Gastroenterology2015，21，12： | 7 |
| 5 | A meta-analysis of soluble suppression of tumorigenicity 2 (sST2) and clinical outcomes in pulmonary hypertension显示文摘 | King Sum Luk Christina Ip Meng-Qi GONG Sunny Hei WONG William KK Wu Mei DONG Guang-Ping LI Ka Pang Chan Yi-Mei DU Tong LIU Martin CS Wong David Shu Cheong Hui Gary Tse | 2017 | Journal of Geriatric Cardiology2017，14，12： | 5 |
| 6 | 中枢神经系统PPAR-γ在调节能量平衡中的作用显示文摘过氧化物酶体增殖物激活受体-γ(PPAR-γ)是一种核受体,可被脂质激活,诱导参与脂质和葡萄糖代谢旧关基因的表达,因此它是可将营养成分信号启动代谢程序的中介。PPAR-γ是噻唑烷二酮(TZD)类胰岛素噌敏药物的作用靶点,这种药物广泛用于治疗2型糖尿病。TZDs的一种常见副作用是体重增加。之前, | Ryan KK Li B Grayson BE 李兴 秦旭平 | 2011 | 中南医学科学杂志2011，39，3： | 2 |
| 7 | Airway assessment by volumetric computed tomography in snorers and subjects with obstructive sleep apnea in a Far-East Asian population(Chinese)显示文摘 | Chen NH Li KK Li SY | 2002 | Laryngoscope2002，112，4： | 1 |
| 8 | The importance of mandibular position in microvascular mandibular reconstruction 显示文摘 | Li KK Cheney ML Teknos TN | 1996 | The Laryngoscope1996，106，7： | 1 |
| 9 | When should we define the response in the treatment of bone metastases by palliative radiotherapy 显示文摘 | Li KK Hadi S Kirou-Mauro A | 2008 | Clin Oncol2008，20，1： | 1 |
| 10 | Peroxisome proliferator- activated receptor ligands inhibit development of atherosclerosis in LDL receptor-deficient mice 显示文摘 | Li AC Brown KK Silvestre M J | 2000 | Journal of Clinical Investigation2000，106，： | 1 |
| 11 | Retention of a composite dressing for aveo- lopalatal wounds 显示文摘 | Li KK Mulliken JB | 1995 | Plast Reconstr Surg1995，95，4： | 1 |
| 12 | Acquired Fanconi syndromeassociated with prolonged adefovir dipivoxil therapy in achronic hepatitis B patient显示文摘 | Law ST Li KK Ho YY | 2013 | Am J Ther2013，20，6： | 1 |
| 13 | Modified uvulopalatopharyngoplasty: the extended uvulopalatal flap 显示文摘 | Li HY Li KK Chen NH Wang PC | 2003 | Am JOtolaryngol2003，24，： | 1 |
| 14 | Distraction osteogenesis in adult obstructive sleep apnea surgery:a preliminary report显示文摘 | Li KK Powell NB Riley RW et a1 | 2002 | J Oral Maxillofac Surg2002，60，1： | 1 |
| 15 | Surgery and obstructive sleep apnea:lougteam clinical outcome显示文摘 | Riely RW Powell NB Li KK | 2000 | Otolaryngol Head Neck Surg2000，122，： | 1 |
| 16 | Prognostic indicators of IgA nephropathy in the Chinese-clinical and pathological perspectives显示文摘 | Li PK Ho KK Szeto CC | 2002 | Nephrol Dial Transplant2002，17，： | 1 |
| 17 | Myelopathy caused by ossification of ligamentum flavum显示文摘 | Chung OM Chang YP So YC | 2002 | Spine2002，27，12： | 1 |
| 18 | Location of the descending palatine artery in relation to the Le fort 1 osteotomy显示文摘 | Li KK Meara JG Alexander A | 1996 | J Oral Maxillofac Surg1996，54，7： | 1 |
| 19 | Activation of the JNK pathway promotes phosphorylation and degradation of BimEL--a novel mechanism of chemoresistance in T-cell acute lymphoblastic leukemia显示文摘 | Leung KT Li KK Sun SS Chan PK | 2008 | Carcinogenesis2008，29，3： | 1 |
| 20 | Myelopathy caused by ossification of ligamentum flavum显示文摘 | Li KK Chung OM Chang YP | 2002 | Spine2002，27，12： | 1 |
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