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		题名	作者	年代	出处	被引量
	1	Improvement of Drought Tolerance in Transgenic Tobacco Plants by a Dehydrin-Like Gene Transfer显示文摘A full-length cDNA of dehydrin BcDh2 from Boea crassifolia and its antisense nucleotidesequence have been transferred into tobacco (Nicotiana tabacum) NC89 under the controlof a caulifower mosaic virus 35S promoter. Under a progressive water stress, photosyntheticrate, transpiration rate and stomatal conductance of the sense and antisense plantsreduced, and those of the control reduced much more. Photosynthetic rate, transpirationrate and stomatal conductance of all plants tested increased significantly 24 hourslater after recoveried water supply, and those of the sense and antisense plants werehigher than control. These indicated that overexpression of a dehydrin gene in tobaccomay improve tolerance to water stress for plants, however, antisense BcDh2 gene intransgenic plant did not influence physiological conditions. The results of germinationexperiment of the transgenic seeds showed that on MS medium with different concentrationPEG (8000), sense seed could more endure drought than control, while antisense seed wassensitive to drought. The results suggested that the overexpression of a dehydrin genein tobacco might improve the tolerance to water stress for plants.	SHENYe JIAWei-long ZHANGYan-qin HUYuan-lei WUQi LINZhongping	2004	Agricultural Sciences in China2004，3，8：	5
	2	Anchored PCR (A-PCR): A new method for chromosome walking显示文摘	CHENBojun SUNChao WANGYong HUYuanlei LINZhongping	2004	Chinese Science Bulletin2004，49，16：	1
	3	Allergenicity assay of allergen from Dermatophagoides farinae in transgenic tobacco显示文摘Derf2 gene for one of mite allergens in Dermatophagoides farinae has been cloned and expressed under regulation of 35S promoter in transgenic tobacco. The tran-scriptional analysis showed that this mite complete gene structure in genomic sequence could be spliced at prediction site. Allergenicity assay with immunological sera indicated that the extracts from the transgenic tobacco gave obvious positive IgE binding reaction with specific serum pool. This work would be of potential use in allergenicity assessment of genetically modified food.	TANGMingjuan SHENYe HUYuanlei CAOLei NITing ZHANGHongyu LINZhongping	2004	Chinese Science Bulletin2004，49，5：	0

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