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| 1 | Molecular Characterization of a Leaf Senescence-Related Transcription Factor BrWRKY75 of Chinese Flowering Cabbage显示文摘WRKY is a plant-specific transcription factor(TF) involved in the regulation of many biological processes; however, its role in leaf senescence of leafy vegetables remains unknown. In the present work, a WRKY TF, termed Br WRKY75 was isolated from Chinese flowering cabbage [Brassica rapa L. ssp. chinensis(L.) Mokino var. utilis Tsen et Lee]. Analysis of deduced amino acid sequence and the phylogenetic tree showed that Br WRKY75 has high homology with WRKY75 from Brassica oleracea and Arabidopsis thaliana, and belongs to the II c sub-group. Sub-cellular localization and transcriptional activity analysis revealed that Br WRKY75 is a nuclear protein with transcriptional repression activity, and was up-regulated during leaf senescence. Electrophoretic mobility shift assay confirmed that Br WRKY75 directly bound to the W-box(TTGAC) cis-element. Collectively,these results provide a basis for further investigation of the transcriptional regulation of Chinese flowering cabbage leaf senescence. | TAN Xiaoli FAN Zhongqi LI Lulu WU Ya KUANG Jianfei LU Wangjin CHEN Jianye | 2016 | Horticultural Plant Journal2016，2，5： | 11 |
| 2 | Auxin response factor 6A regulates photosynthesis,sugar accumulation,and fruit development in tomato显示文摘Auxin response factors(ARFs)are involved in auxin-mediated transcriptional regulation in plants.In this study,we performed functional characterization of SlARF6A in tomato.SlARF6A is located in the nucleus and exhibits transcriptional activator activity.Overexpression of SlARF6A increased chlorophyll contents in the fruits and leaves of tomato plants,whereas downregulation of SlARF6A decreased chlorophyll contents compared with those of wild-type(WT)plants.Analysis of chloroplasts using transmission electron microscopy indicated increased sizes of chloroplasts in SlARF6A-overexpressing plants and decreased numbers of chloroplasts in SlARF6A-downregulated plants.Overexpression of SlARF6A increased the photosynthesis rate and accumulation of starch and soluble sugars,whereas knockdown of SlARF6A resulted in opposite phenotypes in tomato leaves and fruits.RNA-sequence analysis showed that regulation of SlARF6A expression altered the expression of genes involved in chlorophyll metabolism,photosynthesis and sugar metabolism.SlARF6A directly bound to the promoters of SlGLK1,CAB,and RbcS genes and positively regulated the expression of these genes.Overexpression of SlARF6A also inhibited fruit ripening and ethylene production,whereas downregulation of SlARF6A increased fruit ripening and ethylene production.SlARF6A directly bound to the SAMS1 promoter and negatively regulated SAMS1 expression.Taken together,these results expand our understanding of ARFs with regard to photosynthesis,sugar accumulation and fruit development and provide a potential target for genetic engineering to improve fruit nutrition in horticulture crops. | Yujin Yuan Xin Xu Zehao Gong Yuwei Tang Mengbo Wu Fang Yan Xiaolan Zhang Qian Zhang Fengqing Yang Xiaowei Hu Qichen Yang Yingqing Luo Lihua Mei Wenfa Zhang Cai-Zhong Jiang Wangjin Lu Zhengguo Li Wei Deng | 2019 | Horticulture Research2019，6，1： | 3 |
| 3 | A Banana PHD-Type Transcription Factor MaPHD1 Represses a Cell Wall-Degradation Gene MaXTH6 during Fruit Ripening显示文摘Plant homeobox domain(PHD)-type transcription factors(TFs) are involved in a variety of biological processes. However, its involvement in commercially important fruit ripening process remains largely unclear. In the present work, the characterization of a PHD-type TF termed MaPHD1 from banana fruit is reported. Multiple alignments of the deduced amino acid sequence revealed that Ma PHD1 showed a high homology with Arabidopsis thaliana Alfin1-like proteins belonging to plant-specific sub-family of PHD finger proteins. MaPHD1 was found localized in the nucleus and exhibited trans-repression ability. It was down-regulated by ethylene and ripening. Electrophoretic Mobility Shift Assay(EMSA) and transient expression analysis demonstrated that Ma PHD1 directly bound to the G-rich motifs in the promoter of Ma XTH6, which is associated with cell wall degradation, and subsequently repressed its expression. These findings suggest that MaPHD1 may be negatively associated with banana fruit ripening, at least in part, by the direct suppression of Ma XTH6. Taken together, these findings provide new insights into the transcriptional regulatory networks of banana fruit ripening. | WEI Wei FAN Zhongqi CHEN Jianye KUANG Jianfei LU Wangjin SHAN Wei | 2017 | Horticultural Plant Journal2017，3，5： | 2 |
| 4 | Effects of reactive oxygen species on cellular wall disassembly of banana fruit during ripening显示文摘 | Guiping Cheng Xuewu Duan John Shi Wangjin Lu Yunbo Luo Weibo Jiang Yueming Jiang | 2008 | Food Chemistry2008，，2： | 1 |
| 5 | Methionine oxidation and reduction of the ethylene signaling component MaEIL9 are involved in banana fruit ripening显示文摘The ethylene insensitive 3/ethylene insensitive3-like(EIN3/EIL)plays an indispensable role in fruit ripening.However,the regulatory mechanism that links post-translational modification of EIN3/EIL to fruit ripening is largely unknown.Here,we studied the expression of 13 MaE IL genes during banana fruit ripening,among which MaE IL9 displayed higher enhancement particularly in the ripening stage.Consistent with its transcript pattern,abundance of MaE IL9 protein gradually increased during the ripening process,with maximal enhancement in the ripening.DNA affinity purification(DAP)-seq analysis revealed that MaE IL9 directly targets a subset of genes related to fruit ripening,such as the starch hydrolytic genes MaA MY3D and MaB AM1.Stably overexpressing MaE IL9 in tomato fruit hastened fruit ripening,whereas transiently silencing this gene in banana fruit retarded the ripening process,supporting a positive role of MaEIL9 in fruit ripening.Moreover,oxidation of methionines(Met-129,Met-130,and Met-282)in MaEIL9 resulted in the loss of its DNA-binding capacity and transcriptional activation activity.Importantly,we identified MaEIL9 as a potential substrate protein of methionine sulfoxide reductase A MaMsrA4,and oxidation of Met-129,Met-130,and Met-282in MaEIL9 could be restored by MaMsrA4.Collectively,our findings reveal a novel regulatory network controlling banana fruit ripening,which involves MaMsrA4-mediated redox regulation of the ethylene signaling component MaEIL9. | Lisha Zhu Lin Chen Chaojie Wu Wei Shan Danling Cai Zengxiang Lin Wei Wei Jianye Chen Wangjin Lu Jianfei Kuang | 2023 | Journal of Integrative Plant Biology2023，65，1： | 1 |
| 6 | Ethylene-influenced flower opening and expression of genes encoding Etrs , Ctrs , and Ein 3 s in two cut rose cultivars显示文摘 | Hui Tan Xiaohui Liu Nan Ma Jingqi Xue Wangjin Lu Jinghe Bai Junping Gao | 2006 | Postharvest Biology and Technology2006，，2： | 1 |
| 7 | 1-MCP delayed soften- ing and affected expression of XET and EXP genes in harvested cherimoya fruit显示文摘 | LI Cairong SHEN Weibing LU Wangjin | 2009 | Postharvest Biology and Technology2009，52，3： | 1 |
| 8 | One- dimensional conducting polymer nanocomposites : Synthesis, properties and applications显示文摘 | LU X F WANGJIN ZHANG W J WANG C | 2011 | Progress in Pol-ymer Science2011，36，： | 1 |
| 9 | Mitogen-activated protein kinase 14-mediated phosphorylation of MaMYB4 negatively regulates banana fruit ripening显示文摘Mitogen-activated protein kinase(MAPK/MPK)cascades play crucial parts in plant growth,development processes,immune ability,and stress responses;however,the regulatory mechanism by which MAPK affects fruit ripening remains largely unexplored.Here,we reported that MaMPK14 cooperated with MaMYB4 to mediate postharvest banana fruit ripening.Transient overexpression of individual MaMPK14 and MaMYB4 in banana fruit delayed fruit ripening,confirming the negative roles in the ripening.The ripening negative regulator MaMYB4 could repress the transcription of genes associated with ethylene biosynthesis and fruit softening,such as MaACS1,MaXTH5,MaPG3,and MaEXPA15.Furthermore,MaMPK14 phosphorylated MaMYB4 at Ser160 via a direct interaction.Mutation at Ser160 of MaMYB4 reduced its interaction with MaMPK14 but did not affect its subcellular localization.Importantly,phosphorylation of MaMYB4 by MaMPK14 enhanced the MaMYB4-mediated transcriptional inhibition,binding strength,protein stability,and the repression of fruit ripening.Taken together,our results delineated the regulation pathway of MAPK module during banana fruit ripening,which involved the phosphorylation modification of MaMYB4 mediated by MaMPK14. | Yingying Yang Chaojie Wu Wei Shan Wei Wei Yating Zhao Jianfei Kuang Jianye Chen Yueming Jiang Wangjin Lu | 2023 | Horticulture Research2023，10，1： | 0 |
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