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| 1 | Ephrin-B2 is differentially expressed in the intestinal epithelium in Crohn's disease and contributes to accelerated epithelial wound healing in vitro显示文摘AIM: Eph receptor tyrosine kinases and their membrane bound receptor-like ligands, the ephrins, represent a bi-directional cell-cell contact signaling system that directs epithelial movements in development. The meaning of this system in the adult human gut is unknown. We investigated the Eph/ephrin mRNA expression in the intestinal epithelium of healthy controls and patients with inflammatory bowel disease (IBD).METHODS: mRNA expression profiles of all Eph/ephrin family members in normal small intestine and colon were established by real-time RT-PCR. In addition, differential expression in IBD was investigated by cDNA array technology, and validated by both real-time RT-PCR and immunohistochemistry. Potential effects of enhanced EphB/ephrin-B signaling were analyzed in an in vitro IEC-6 cell scratch wound model.RESULTS: Human adult intestinal mucosa exhibits a complex pattern of Eph receptors and ephrins. Beside the known prominent co-expression of EphA2 and ephrinA1,we found abundantly co-expressed EphB2 and ephrin-B1/2.Interestingly, cDNA array data, validated by real-time PCR and immunohistochemistry, showed upregulation of ephrin-B2 in both perilesional and lesional intestinal epithelial cells of IBD patients, suggesting a role in epithelial homeostasis. Stimulation of ephrin-B signaling in ephrinB1/2 expressing rat IEC-6-cells with recombinant EphB1Fc resulted in a significant dose-dependent acceleration of wound closure. Furthermore, fluorescence microscopy showed that EphB1-Fc induced coordinated migration of wound edge cells is associated with enhanced formation of lamellipodial protrusions into the wound, increased actin stress fiber assembly and production of laminin at the wound edge.CONCLUSION: EphB/ephrin-B signaling might represent a novel protective mechanism that promotes intestinal epithelial wound healing, with potential impact on epithelial restitution in IBD. | Christian Hafner Stefanie Meyer Thomas Langmann Gerd Schmitz Frauke Bataille Ilja Hagen Bernd Becker Alexander Roesch Gerhard Rogler Michael Landthaler Thomas Vogt | 2005 | World Journal of Gastroenterology2005，11，26： | 9 |
| 2 | A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing显示文摘 | Pablo Landgraf Mirabela Rusu Robert Sheridan Alain Sewer Nicola Iovino Alexei Aravin Sébastien Pfeffer Amanda Rice Alice O. Kamphorst Markus Landthaler Carolina Lin Nicholas D. Socci Leandro Hermida Valerio Fulci Sabina Chiaretti Robin Foà Julia Schliwka | 2007 | Cell2007，，7： | 4 |
| 3 | Ephrin-B reverse signaling induces expression of wound healing associated genes in IEC-6 intestinal epithelial cells显示文摘AIM: Eph receptors and ephrin ligands play a pivotal role in development and tissue maintenance. Since previous data have indicated an involvement of ephrin-B2 in epithelial healing, we investigated the gene expression and downstream signaling pathways induced by ephrin-B mediated cell-cell signaling in intestinal epithelial cells.METHODS: Upon stimulation of ephrin-B pathways in IFC-6 cells with recombinant rat EphB1-Fc, gene expression was analyzed by Affymetrix(R) rat genome 230 high density arrays at different time points. Differentially expressed genes were confirmed by real-time RT-PCR. In addition, MAP kinase pathways and focal adhesion kinase (FAK) activation downstream of ephrin-B were investigated by immunoblotting and fluorescence microscopy.RESULTS: Stimulation of the ephrin-B reverse signaling pathway in IEC-6 cells induces predominant expression of genes known to be involved into wound healing/cell migration, antiapoptotic pathways, host defense and inflammation. Cox-2, c-Fos, Egr-1, Egr-2, and MCP-1 were found among the most significantly regulated genes.Furthermore, we show that the expression of repairrelated genes is also accompanied by activation of the ERK1/2 MAP kinase pathway and FAK, two key regulators of epithelial restitution.CONCLUSION: Stimulation of the ephrin-B reverse signaling pathway induces a phenotype characterized by upregulation of repair-related genes, which may partially be mediated by ERK1/2 pathways. | Christian Hafner Stefanie Meyer Ilja Hagen Bernd Becker Alexander Roesch Michael Landthaler Thomas Vogt | 2005 | World Journal of Gastroenterology2005，11，29： | 2 |
| 4 | Identification of novel argonaute - associated proteins 显示文摘 | MEISTER G LANDTHALER M PETERS L | 2005 | Curr Biol2005，15，23： | 1 |
| 5 | Non-oncologic indications for ALA-PDT显示文摘 | Szeimies RM Landthaler M Karrer S | 2002 | J-Dermatolog-Treat2002，13，1： | 1 |
| 6 | Photodynamic therapy in dermatology显示文摘 | Babilas P Landthaler M Szeimies R-M | 2006 | Journal of the American Academy of Dermatology2006，16，4： | 1 |
| 7 | Spindle cell hemangioma显示文摘 | Coras B Hohenleutner U Landthaler M | 2003 | Dermatol Surg2003，29，8： | 1 |
| 8 | Sequence-spe- cific inhibition of microRNA-and siRNA-induced RNA si- lencing显示文摘 | Meister G Landthaler M Dorsett Y | 2004 | Rna2004，10，3： | 1 |
| 9 | A new staron the H2 O2 rizon of wound healing- 显示文摘 | Schreml S Landthaler M Schaferling M Babilas P | 2011 | Exp Dermatol2011，20，： | 1 |
| 10 | Sequence-specific inhibition of microRNA-and siRNA-induced RNA silencing显示文摘 | Meister G Landthaler M Dorsett Y | 2004 | RNA2004，10，3： | 1 |
| 11 | Endogenous MHC class Ⅱ processing of a viral nuclear antigen after autophagy显示文摘 | Paludan C Schmid D Landthaler M | 2005 | Science2005，307，5709： | 1 |
| 12 | Detection of differentially regulated genes in keratinocytes by cDNA array hybridization:Hsp27 and other novel players in response to artificial ultraviolet radiation显示文摘 | Becker B Vogt T Landthaler M | 2001 | J Invest Dermatol2001，116，6： | 1 |
| 13 | Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs显示文摘 | MEISTER G LANDTHALER M PATKANIOWSKA A | 2004 | Mol Cell2004，15，： | 1 |
| 14 | A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing显示文摘 | Pablo Landgraf Mirabela Rusu Robert Sheridan Alain Sewer Nicola Iovino Alexei Aravin Sébastien Pfeffer Amanda Rice Alice O. Kamphorst Markus Landthaler Carolina Lin Nicholas D. Socci Leandro Hermida Valerio Fulci Sabina Chiaretti Robin Foà Julia Schliwka | 2007 | Cell2007，，7： | 1 |
| 15 | Pityriasis Rubra Pilaris 显示文摘 | Klein A Landthaler M Karrer S | 2010 | Ameri- can journal of elinieal dermatology2010，11，3： | 1 |
| 16 | DGCR8 -dependent mi- croRNA biogenesis is essential for skin development显示文摘 | Yi R Pasolli HA Landthaler M | 2009 | Proc Natl Acad Sci USA2009，106，2： | 1 |
| 17 | Laser therapy of vascular lesions显示文摘 | Landthaler M Hohenleutner U | 2006 | Photodermatol Photoimmunol Photomed2006，22，6： | 1 |
| 18 | Endogenous MHC class II processing of a viral nuclear antigen after autophagy显示文摘 | Paludan C Schmid D Landthaler M Vockerodt M Kube D Tuschl T Münz C | 2005 | Science2005，307，5709： | 1 |
| 19 | Endogenous MHC class II processing of a viral nuclear antigen after autophagy显示文摘 | Paludan C Schmid D Landthaler M | 2005 | Science2005，307，5709： | 1 |
| 20 | Endogenous MHC class Ⅱ processing of a viral nuclear antigen after autophagy显示文摘 | Paludan C Schmid D Landthaler M | 2005 | Science2005，307，5709： | 1 |
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