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| 1 | Human umbilical cord Wharton's Jelly-derived mesenchymal stem cells differentiation into nerve-like cells显示文摘Background The two most basic properties of mesenchymal stem cells (MSCs) are the capacities to self-renew indefinitely and differentiate into multiple cells and tissue types. The cells from human umbilical cord Wharton’s Jelly have properties of MSCs and represent a rich source of primitive cells. This study was conducted to explore the possibility of inducing human umbilical cord Wharton’s Jelly-derived MSCs to differentiate into nerve-like cells.Methods MSCs were cultured from the Wharton’s Jelly taken from human umbilical cord of babies delivered after full-term normal labor. Salvia miltiorrhiza and β-mercaptoethanol were used to induce the human umbilical cord-derived MSCs to differentiate. The expression of neural protein markers was shown by immunocytochemistry. The induction process was monitored by phase contrast microscopy, electron microscopy (EM), and laser scanning confocal microscopy (LSCM) .The pleiotrophin and nestin genes were measured by reverse transcription-polymerase chain reaction (RT-PCR). Results MSCs in the Wharton’s Jelly were easily attainable and could be maintained and expanded in culture. They were positive for markers of MSCs, but negative for markers of hematopoietic cells and graft-versus-host disease (GVHD)-related cells. Treatment with Salvia miltiorrhiza caused Wharton’s Jelly cells to undergo profound morphological changes. The induced MSCs developed rounded cell bodies with multiple neurite-like extensions. Eventually they developed processes that formed networks reminiscent of primary cultures of neurons. Salvia miltiorrhiza and β-mercaptoethanol also induced MSCs to express nestin, β-tubulinⅢ, neurofilament (NF) and glial fibrillary acidic protein (GFAP). It was confirmed by RT-PCR that MSCs could express pleiotrophin both before and after induction by Salvia miltiorrhiza. The expression was markedly enhanced after induction and the nestin gene was also expressed.Conclusions MSCs could be isolated from human umbilical cord Wharton’s Jelly. They were capable of differentiating into nerve-like cells using Salvia miltiorrhiza or β-mercaptoethanol. The induced MSCs not only underwent morphologic changes, but also expressed the neuron-related genes and neuronal cell markers. They may represent an alternative source of stem cells for central nervous system cell transplantation. | MA Lian FENG Xue-yong CUI Bing-lin Frieda Law JIANG Xue-wu YANG Li-ye XIE Qing-dong HUANG Tian-hua | 2005 | Chinese Medical Journal2005，，23： | 103 |
| 2 | Prevention and management of hepatitis B virus reactivation in patients with hematological malignancies treated with anticancer therapy显示文摘Hepatitis due to hepatitis B virus(HBV) reactivation can be severe and potentially fatal, but is preventable. HBV reactivation is most commonly reported in patients receiving cancer chemotherapy, especially rituximabcontaining therapy for hematological malignancies and those receiving stem cell transplantation. All patients with hematological malignancies receiving anticancer therapy should be screened for active or resolved HBV infection by blood tests for hepatitis B surface antigen(HBs Ag) and antibody to hepatitis B core antigen(antiHBc). Patients found to be positive for HBs Ag should be given prophylactic antiviral therapy to prevent HBV reactivation. For patients with resolved HBV infection, no standard strategy has yet been established to prevent HBV reactivation. There are usually two options. One is pre-emptive therapy guided by serial HBV DNA monitoring, whereby antiviral therapy is given as soon as HBV DNA becomes detectable. However, there is little evidence regarding the optimal interval and period of monitoring. An alternative approach is prophylactic antiviral therapy, especially for patients receiving highrisk therapy such as rituximab, newer generation of anti-CD20 monoclonal antibody, obinutuzumab or hematopoietic stem cell transplantation. This strategy may effectively prevent HBV reactivation and avoid the inconvenience of repeated HBV DNA monitoring. Entecavir or tenofovir are preferred over lamivudine as prophylactic therapy. Although there is no well-defined guideline on the optimal duration of prophylactic therapy, there is growing evidence to recommend continuing prophylactic antiviral therapy for at least 12 mo after cessation of chemotherapy, and even longer for those who receive rituximab or who had high serum HBV DNA levels before the start of immunosuppressive therapy. Many novel agents have recently become available for the treatment of hematological malignancies, and these agents may be associated with HBV reactivation. Although there is currently limited evidence to guide the optimal preventive measures, we recommend antiviral prophylaxis in HBs Ag-positive patients receiving novel treatments, especially the Bruton tyrosine kinase inhibitors and the phosphatidylinositol 3-kinase inhibitors, which are B-cell receptor signaling modulators and reduce proliferation of malignant B-cells. Further studies are needed to clarify the risk of HBV reactivation with these agents and the best prophylactic strategy in the era of targeted therapy for hematological malignancies. | Man Fai Law Rita Ho Carmen KM Cheung Lydia HP Tam Karen Ma Kent CY So Bonaventure Ip Jacqueline So Jennifer Lai Joyce Ng Tommy HC Tam | 2016 | World Journal of Gastroenterology2016，22，28： | 10 |
| 3 | Effect of epidermal growth factor and dexamethasone on fetal rat lung development显示文摘 | MA Lian WANG Ai-hong Law Frieda HE Hong-yan MA Gui-xia WANG Hong-wu LIN Li-min | 2009 | Chinese Medical Journal2009，，17： | 4 |
| 4 | Studies on kinetics and reuse of spent liquor in the TEMPO-mediated selective oxidation of mechanical pulp 显示文摘 | Mao Lianshan Ma Pu Law K | 2010 | Ind Eng Chem Res2010，49，： | 1 |
| 5 | Risk factors for in- cident hyperuricemia during mid-adulthood in African American and white men and women enrolled in the ARIC cohort study 显示文摘 | McAdams-DeMarco MA Law A Maynard JW | 2013 | BMC musculoskeletal disorders2013，14，： | 1 |
| 6 | Hamartoma of the breast:a clinicopathological review显示文摘 | Tse GM Law BK Ma TK | 2002 | J Clin Pathol2002，55，： | 1 |
| 7 | Recent advances in understanding enteric pathogenic Escherichia coli 显示文摘 | Croxen MA Law RJ Scholz R | 2013 | Clin Microbiol Rev2013，26，4： | 1 |
| 8 | Study of conformation of vicilin from Dolichos lablab and Phaseolus calcaratus by Fourier-transform infrared spectroscopy and differential scanning calorimetry显示文摘 | Law H Y Choi S M Ma C Y | 2008 | Food Research International2008，7，： | 1 |
| 9 | Exploration of the factorial structure of the revised personal functions of the volunteerism scale for Chinese adolescents 显示文摘 | Law B M F Shek D T L Ma C M S | 2010 | Social Indicators Research2010，100，3： | 1 |
| 10 | Interdependent SMAD and JNK signaling in transforming growth factor -β- mediated transcription显示文摘 | Engel ME McDonnell MA Law BK | 1999 | J Biol Chem1999，274，37： | 1 |
| 11 | Recent advances in understand- ing enteric pathogenic Escherichia coli 显示文摘 | Croxen MA Law RJ Scholz R | 2013 | Clin Microbiol Rev2013，26，： | 1 |
| 12 | Hamartoma of the breast: a clinieopathological review显示文摘 | Tse GM Law BK Ma TK | 2002 | J Clin Pathol2002，55，12： | 1 |
| 13 | Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp显示文摘 | Nie DY Ma QH Law JW | 2006 | Neuron Glia Biol2006，2，： | 1 |
| 14 | Pulmonary artery stents :long-term follow-up 显示文摘 | Law MA Shamszad P Nugent AW | 2010 | Catheter Cardiovasc Interv2010，75，5： | 1 |
| 15 | Interdependent SMAD and JNK signaling in transforming growth factor-β-mediated transcription显示文摘 | Engel ME Mcdonnell MA Law BK | | 0，，52： | 1 |
| 16 | Position estimation using ultrawideband time difference of arrival measurements显示文摘 | Xu J Ma M Law C L | 2008 | IET Sci Meas Technol Metal2008，2，1： | 1 |
| 17 | Frailty as a novel predictor of mortality and hospitalizationin individuals of all ages undergoing hemodialysis显示文摘 | McAdams-DeMarco MA Law A Salter ML | 2013 | J Am GeriatrSoc2013，61，6： | 1 |
| 18 | Reeent advances in understanding enteric pathogenic escheriehia coli显示文摘 | Croxen MA Law RJ Scholz R ct al | 2013 | Clin Microbiol Rev2013，26，4： | 1 |
| 19 | Optimization of headspace solid phase microextraction (HS-SPME) for gas chromatography mass spectrometry (GC–MS) analysis of aroma compounds in cooked beef using response surface methodology显示文摘 | Q.L. Ma N. Hamid A.E.D. Bekhit J. Robertson T.F. Law | 2013 | Microchemical Journal2013，，： | 1 |
| 20 | Atrial septostomy improves survival in select patients with pulmonary hypertens- ion显示文摘 | Law MA Grifka RG Mullins CE | 2007 | Am Heart J2007，153，5： | 1 |
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