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| 1 | Establishment and characterization of four human hepatocellular carcinoma cell lines containing hepatitis B virus DNA显示文摘INTRODUCTIONHepatocelularcarcinoma(HCC)isoneofthemostprevalentmalignantdiseasesencounteredintheworld,kilingupto1milionpeoplea... | Jae Ho Lee 1, Ja Lok Ku 1, Young Jin Park 1,2 , Kuhn Uk Lee 2, Woo Ho Kim 3 and Jae Gahb Park 1,2 | 1999 | World Journal of Gastroenterology1999，5，4： | 28 |
| 2 | Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity显示文摘AIM:To observe the effects of traditional anti-inflammatory medicine Lonicerae Flos (LF) on rat reflux esophagitis (RE) induced by pylorus and forestomach ligation compared with the well-known proton antioxidant, α-tocopherol.METHODS:Rats were pretreated with three different dosages of LF (500, 250 and 125 mg/kg) orally, once a day for 14 d before pylorus and forestomach ligation. Nine hours after pylorus and forestomach ligation, changes to the stomach and esophagus lesion areas, gastric volumes, acid and pepsin outputs, antioxidant effects, esophageal lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), myeloperoxidase and glutathione (GSH) levels, and collagen contents (marker of flexibility) were observed on the esophageal and fundic histopathology. The results were compared with an α-tocopherol (once orally, 1 h before operation, 30 mg/kg) treated group in which the effects on RE were already conf irmed.RESULTS: Pylorus and forestomach ligations caused marked increases of gross esophageal and gastric mucosa lesion areas, which corresponded with histopathological changes. In addition, increases of esophageal lipid peroxidation, decreases of SOD, CAT, and GSH-free radical scavengers, increases of collagen were observed. However, these pylorus and forestomach ligation induced RE were dose-dependently inhibited by treatment of 500, 250 and 125 mg/kg of LF extract, mediated by antioxidant effects. RE at 250 mg/kg showed similar effects α-tocopherol.CONCLUSION: The results suggest that antioxidant effects of LF could attenuate the severity of RE and prevent the esophageal mucosal damage, and validate its therapeutic use in esophageal reflux disease. | Sae-Kwang Ku Bu-Il Seo Ji-Ha Park Gyu-Yeol Park Young-Bae Seo Jae-Soo Kim Hyeung-Sik Lee Seong-Soo Roh | 2009 | World Journal of Gastroenterology2009，15，38： | 19 |
| 3 | Magnetic resonance evaluation of human mesenchymal stem cells in corpus cavernosa of rats and rabbits显示文摘瞄准:为了调查超级顺磁的氧化铁(SPIO ) 的生物进程是否把人的间充质的干细胞(hMSCs ) 标记,可以被使用在活体内 non-invasively 监视有在老鼠和兔子的阴茎海绵体的常规 1.5-T 系统考试的磁性的回声(先生) 成像。方法:分别地与普鲁士的蓝色和 Tripan 蓝色检验的标记 SPIO 的 hMSCswere 的标记的效率和生存能力。在标记 SPIO 的 hMSCs 被移植到老鼠和兔子的阴茎海绵体以后,连续 T2 加权的先生图象被拿,组织学的考试在一个 4 星期的时期上被执行。结果:与 SPIOcompared 装载到未标记的房间的 hMSCs 有一个类似的生存能力。为标记 SPIO 的 hMSCs 超过 1 x 10 ~ 5concentration 在试管内,先生图象在信号紧张显示出减少。在在老鼠和兔子阴茎海绵体的标记 SPIO 的 hMSCs 的区域的先生信号紧张局部地减少了并且被限制。在进阴茎海绵体的标记 SPIO 的 hMSCs 的注射以后,先生成像证明 hMSCs 能在注射以后被看见至少 12 个星期。铁的存在被证实,普鲁士的蓝色在组织学的节染色。结论:在老鼠和兔子的阴茎海绵体的标记 SPIO 的 hMSCs 能被分子的先生成像 non-invasively 评估。我们的调查结果建议先生成像有能力在可勃起的机能障碍的背景在动物测试 hMSCs 的长期的治疗学的潜力。 | Yun-Seob Song Ja-Hyeon Ku Eun-Seop Song Jung-Hoon Kim Jin-Suck Jeon Kong-Hee Lee Sook-Ja Kim Hee-Jeong Cheong Ik-Sung Lim Dongho Choi Jong-Ho Won | 2007 | Asian Journal of Andrology2007，9，3： | 11 |
| 4 | Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method显示文摘AIM: To enhance the differentiation of insulin producing cell (IPC) ability from embryonic stem (ES) cells in vitro. METHODS: Four-day embryoid body (EB)-formatted ES cells were dissociated as single cells for the followed plasmid DNA delivery. The use of NucleofectorTM electroporator (Amaxa biosystems, Germany) in combination with medium-contained G418 provided a high efficiency of gene delivery for advanced selection. Neucleofected cells were plated on the top of fibronectincoated Petri dishes. Addition of Ly294002 and raised the glucose in medium at 24 h before examination.The differentiation status of these cells was monitored by semi-quantitative PCR (SQ-PCR) detection of the expression of relative genes, such as oct-4, sox-17, foxa2, mixl1, pdx-1, insulin 1, glucagons and somatostatin. The percentage of IPC population on d 18 of the experiment was investigated by immunohistochemistry (IHC), and the content/secretion of insulin was estimated by ELISA assay. The mice with severe combined immunodeficiency disease (SCID) pretreated with streptozotocin (STZ) were used to eliminate plasma glucose restoration after pax4+ ES implantation. RESULTS: A high efficiency of gene delivery was demonstrated when neucleofection was used in the present study; approximately 70% cells showed DsRed expression 2 d after neucleofection. By selection of medium-contained G418, the percentage of DsRed expressing cells kept high till the end of study. The pancreatic differentiation seemed to be accelerated by pax4 nucleofection. When compared to the group of cells with mock control, foxa2, mixl1, pdx1, higher insulin and somatostatin levels were detected by SQ-PCR 4 d after nucleofection in the group of pax4 expressing plasmid delivery. Approximately 55% of neucleofected cells showed insulin expression 18 d after neucleofection, and only 18% of cells showed insulin expression in mock control. The disturbance was shown by nucleofected pax4 RNAi vector; only 8% of cells expressed insulin 18 d after nucleofection. A higher IPC population was also detected in the insulin content by ELISA assay, and the glucose dependency was demonstrated in insulin secretion level. In the animal model, improvement of average plasma glucose concentration was observed in the group of pax-4 expressed ES of SCID mice pretreated with STZ, but no significant difference was observed in the group of STZ-pretreated SCID mice who were transplanted ES with mock plasmid. CONCLUSION: Enhancement of IPC differentiation from EB-dissociated ES cells can be revealed by simply using pax4 expressing plasmid delivery. Not only more IPCs but also pancreatic differentiation-related genes can be detected by SQ-PCR. Expression of relative genes, such as foxa 2, mixl 1, pdx-1, insulin 1 and somatostatin after nucleofection, suggests that pax4 accelerates the whole differentiation progress. The higher insulin production with glucose dependent modulation suggests that pax4 expression can drive more mature IPCs. Although further determination of the entire mechanism is required, thepotential of pax-4-nucleofected cells in medical treatment is promising. | Han-Tso Lin Chung-Lan Kao Kun-Hsiung Lee Yuh-Lih Chang Shih-Hwa Chiou Fu-Ting Tsai Tung-Hu Tsai Dey-Chyi Sheu Larry LT Ho Hung-Hai Ku | 2007 | World Journal of Gastroenterology2007，13，11： | 10 |
| 5 | PD-1 blockade-unresponsive human tumor-infiltrating CD8^(+)T cells are marked by loss of CD28 expression and rescued by IL-15显示文摘Blockade of programmed death-1(PD-1)reinvigorates exhausted CD8^(+)T cells,resulting in tumor regression in cancer patients.Recently,reinvigoration of exhausted CD8^(+)T cells following PD-1 blockade was shown to be CD28-dependent in mouse models.Herein,we examined the role of CD28 in anti-PD-1 antibody-induced human T cell reinvigoration using tumor-infiltrating CD8^(+)T cells(CD8^(+)TILs)obtained from non-small-cell lung cancer patients.Single-cell analysis demonstrated a distinct expression pattern of CD28 between mouse and human CD8^(+)TILs.Furthermore,we found that human CD28^(+)CD8^(+)but not CD28^(–)CD8^(+)TILs responded to PD-1 blockade irrespective of B7/CD28 blockade,indicating that CD28 costimulation in human CD8^(+)TILs is dispensable for PD-1 blockade-induced reinvigoration and that loss of CD28 expression serves as a marker of anti-PD-1 antibody-unresponsive CD8^(+)TILs.Transcriptionally and phenotypically,PD-1 blockade-unresponsive human CD28^(–)PD-1^(+)CD8^(+)TILs exhibited characteristics of terminally exhausted CD8^(+)T cells with low TCF1 expression.Notably,CD28^(–)PD-1^(+)CD8^(+)TILs had preserved machinery to respond to IL-15,and IL-15 treatment enhanced the proliferation of CD28^(–)PD-1^(+)CD8^(+)TILs as well as CD28^(+)PD-1^(+)CD8^(+)TILs.Taken together,these results show that loss of CD28 expression is a marker of PD-1 blockade-unresponsive human CD8^(+)TILs with a TCF1–signature and provide mechanistic insights into combining IL-15 with anti-PD-1 antibodies. | Kyung Hwan Kim Hong Kwan Kim Hyung-Don Kim Chang Gon Kim Hoyoung Lee Ji Won Han Seong Jin Choi Seongju Jeong Minwoo Jeon Hyunglae Kim Jiae Koh Bo Mi Ku Su-Hyung Park Myung-Ju Ahn Eui-Cheol Shin | 2021 | Cellular & Molecular Immunology2021，18，2： | 6 |
| 6 | Sequence Information on Simple Sequence Repeats and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean显示文摘Mungbean(Vigna radiata(L.) Wilczek) is a unique species in its ability to fix atmospheric nitrogen,with early maturity,and relatively good drought resistance.We used 454 sequencing technology for transcriptome sequencing.A total of 150 159 and 142 993 reads produced 5 254 and 6 374 large contigs(≥500 bp) with an average length of 833 and 853 for Sunhwa and Jangan,respectively.Functional annotation to known sequences yielded 41.34% and 41.74% unigenes for Jangan and Sunhwa.A higher number of simple sequence repeat(SSR) motifs was identified in Jangan(1 630) compared with that of Sunhwa(1 334).A similar SSR distribution pattern was observed in both varieties.A total of 8 249 single nucleotide polymorphisms(SNPs) and indels with 2 098 high-confidence candidates were identified in the two mungbean varieties.The average distance between individual SNPs was ~860 bp.Our report demonstrates the utility of transcriptomic data for implementing a functional annotation and development of genetic markers.We also provide large resource sequence data for mungbean improvement programs. | Kyaw Thu Moe Jong-Wook Chung Young-Il Cho Jung-Kyung Moon Ja-Hwan Ku Jin-Kyo Jung Jungran Lee Yong-Jin Park | 2011 | Journal of Integrative Plant Biology2011，53，1： | 4 |
| 7 | 通过生态博物馆建设保护韩国乡村农业文化遗产(英文)显示文摘乡村文化遗产包括农业文化遗产,对其进行正确的保护可以增强社区认同感。本文通过对生态博物馆建设等问题进行探索,试图寻找到适合乡村文化遗产保护的方法。文章首先梳理了生态博物馆及乡村文化遗产的基本概念;然后,在分析了生态博物馆的概念、全球重要农业文化遗产的内涵、欧洲乡村文化遗产保护政策以及韩国农业文化遗产基本特征的基础上,定义了韩国乡村生态博物馆的概念与内涵。本研究提出了适用于韩国乡村生态博物馆建设的一系列具体方法,包括具体的组成与分类等。最后,本文尝试将生态博物馆的理念与方法应用于韩国农业文化遗产地,选取了韩国青山岛地区和Gurae地区的两个试验地进行尝试。结果表明,生态博物馆建设不仅可以保护农业文化遗产,而且可以扩大应用到更大范围的乡村文化遗产保护。因此,可以说乡村生态博物馆建设不仅有助于农业文化遗产的保护,而且对其周边地区的资源保护也具有不可忽略的重要作用。另外,生态博物馆建设还可以增强社区认同感,促进当地经济的发展。 | Lee Jeong-Hwan Yoon Won-Keun Choi Sik-In Ku Jin-Hyuk | 2016 | Journal of Resources and Ecology2016，7，3： | 4 |
| 8 | Mechanism and management of burr entrapment:A nightmare of interven-tional cardiologists显示文摘Entrapment of the burr within calcified lesion is an uncommon, but serious complication during rotational atherectomy and usually needs surgical retrieval. We report a case series of this complication and also review the possible mechanisms, such as kokesi phenomenon or insufficient pecking motion with decreased rotational speed. We also review the potential techniques ever proposed to rescue this complica-tion percutaneously, including simple manual traction, balloon dilation to release the trap, snaring the burr as distal as possible for forceful local traction and wedging the burr with a child catheter to facilitate retrieval. Gentle pecking motion of the burr for sufficient ablation and shortening the run less than 15 s may avoid such complications. Interventional cardiologists using the rotablator should be familiar with the tips and tricks to avoid and rescue this complication. | Chia-Pin Lin Ji-Hung Wang Wen-Ling Lee Po-Ming Ku Wei-Hsian Yin Ten-Ping Tsao Chi-Jen Chang | 2013 | Journal of Geriatric Cardiology2013，10，3： | 3 |
| 9 | Saeng-Kankunbi-Tang(生肝健脾汤) Protects Liver against Oxidative Damage through Activation of ERK/Nrf2 Pathway显示文摘Objective: To investigate the cytoprotective effects of Saeng-kankunbi-tang(生肝健脾汤, SKT), a herbal prescription consisting of Artemisia capillaris and Alisma canaliculatum, and its underlying mechanism involved. Methods: In mice, blood biochemistry and histopathology were assessed in carbon tetrachloride(CCl4)-induced oxidative hepatic injury in vivo. The animal groups included vehicle-treated control, CCl4, SKT 500 mg/(kg·day) CCl4+SKT 200 or 500 mg/(kg·day). In Hep G2 cell, tert-butyl hydroperoxide(t BHP) induced severe oxidative stress and mitochondrial dysfunction in vitro. The cyto-protective effects of SKT were determined by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide(MTT) assay, fluorescence activated cell sorting analysis and western blotting. Results: The administration of SKT prevented liver damage induced by CCl4 in mice, by inhibition of hepatocyte degeneration and inflammatory cell infiltration as well as plasma parameters such as alanine aminotransferase(P<0.01). Moreover, treatment with t BHP induced hepatocyte death and cellular reactive oxygen species production in hepatocyte cell line. However, SKT pretreatment(30–300 μg/m L) reduced this cell death and oxidative stress(P<0.01). More importantly, SKT inhibited the ability of t BHP to induce changes in mitochondrial membrane transition in cell stained with rhodamine 123(P<0.01). Furthermore, treatment with SKT induced extracellular signal-regulated kinases-mediated nuclear factor erythroid-2-related factor 2(Nrf2) activation as well as the expressions of heme oxygenase 1 and glutamate-cystein ligase catalytic, Nrf2 target genes. Conclusion: SKT has the ability to protect hepatocyte against oxidative stress and mitochondrial damage mediated by Nrf2 activation. | In Woo Lee Hee Yoon Choi Ju-Hee Lee Sun-Dong Park Seung Mo Kim Sae Kwang Ku 赵容杰 Sang Chan Kim Young Woo Kim Hong Sik Choi | 2016 | Chinese Journal of Integrative Medicine2016，22，8： | 3 |
| 10 | Effect of diclofenac sodium on the arachidonic acid cascade显示文摘 | Ku EC Lee W Kothari HV | 1986 | Am J Med1986，80，： | 2 |
| 11 | Anti-inflammatory functions of purpurogallin in LPS-activated human endothelial cells显示文摘 | Kim TH Ku SK Lee IC | | 0，，03： | 1 |
| 12 | A virtual reality system for the assess- ment and rehabilitation of the activities of daily living显示文摘 | Lee JH Ku J Cho W | 2003 | Cy- berpsyehol Behav2003，6，4： | 1 |
| 13 | Association between obesity and BRAFV600E mutation status in patients with papillary thyroid cancer显示文摘 | Lee J Lee CR Ku CR | 2015 | Ann Surg Oncol2015，22，3： | 1 |
| 14 | Structural and electrical properties of sputtered indium–zinc oxide thin films显示文摘 | D.Y. Ku I.H. Kim I. Lee K.S. Lee T.S. Lee J.-h. Jeong B. Cheong Y.-J. Baik W.M. Kim | 2006 | Thin Solid Films2006，，： | 1 |
| 15 | Effects of recombinant adenovirus medi- ated uncoupling protein overexpression on endothelial function and apoptosis显示文摘 | Lee KU Lee 1K Hart J | 2005 | Circ Res2005，96，11： | 1 |
| 16 | Liquefaction performance of soils at the site of a partially completed ground improvement project during the 1999 Chi-Chi Earthquake in Taiwan显示文摘 | LEE D H JUANG C H KU C S | 2001 | Canadian Geoteehnieal Journal2001，，38： | 1 |
| 17 | Predictors of carbon monoxide poi- soning-induced delayed neuropsyehological sequelae 显示文摘 | Ku HL Yang KC Lee YC | 2010 | General Hospital Psychiatry2010，32，3： | 1 |
| 18 | Diagonal recurrent neural network for dynamic systems control 显示文摘 | Ku C C Lee K Y | 1995 | IEEE Trans on NN1995，6，1： | 1 |
| 19 | A novel approach to yard planning under vessel arrival uncertainty显示文摘 | KU L P CHEW E P LEE L H | 2012 | Flexible Services and Manufacturing Journal2012，24，3： | 1 |
| 20 | Comparison of the clinical outcomes between fresh blastoeyst and vitrified - thawed blastocyst transfer显示文摘 | KU P Y LEE R K LIN S Y | 2012 | J Assist Reprod Genet2012，29，12： | 1 |
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