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| 1 | Establishment and characterization of four human hepatocellular carcinoma cell lines containing hepatitis B virus DNA显示文摘INTRODUCTIONHepatocelularcarcinoma(HCC)isoneofthemostprevalentmalignantdiseasesencounteredintheworld,kilingupto1milionpeoplea... | Jae Ho Lee 1, Ja Lok Ku 1, Young Jin Park 1,2 , Kuhn Uk Lee 2, Woo Ho Kim 3 and Jae Gahb Park 1,2 | 1999 | World Journal of Gastroenterology1999，5，4： | 28 |
| 2 | Endoscopic removal of gastric ectopic pancreas:An initial experience with endoscopic submucosal dissection显示文摘AIM:To evaluate the therapeutic usefulness and safety of endoscopic resection in patients with gastric ectopic pancreas.METHODS:A total of eight patients with ectopic pancreas were included.All of them underwent endoscopic ultrasonography before endoscopic resection.Endo-scopic resection was performed by two methods:endo-scopic mucosal resection(EMR)by the injection-and-cut technique or endoscopic mucosal dissection(ESD).RESULTS:We planned to perform EMR in all eight cases but EMR was successful in only four cases.In the other four cases,saline spread into surrounding normal tissues and the lesions becameattened,which made it impossible to remove them by EMR.Inthose four cases,we performed ESD and removed the lesions without any complications.CONCLUSION:If conventional EMR is difficult to remove gastric ectopic pancreas,ESD is a feasible alternative method for successful removal. | Dong Yup Ryu Gwang Ha Kim Do Youn Park Bong Eun Lee Jae Hoon Cheong Dong Uk Kim Hyun Young Woo Jeong Heo Geun Am Song | 2010 | World Journal of Gastroenterology2010，16，36： | 24 |
| 3 | Proton pump inhibitor administration delays rebleeding after endoscopic gastric variceal obturation显示文摘AIM:To clarify the efficacy of proton pump inhibitors(PPIs)after endoscopic variceal obturation(EVO)with N-butyl-2-cyanoacrylate.METHODS:A retrospective study was performed on16 liver cirrhosis patients with gastric variceal bleeding that received EVO with injections of N-butyl-2-cyanoacrylate at a single center(Kyung Hee University Hospital at Gangdong)from January 2008 to December 2012.Medical records including patient characteristics and endoscopic findings were reviewed.Treatment results,liver function,serum biochemistry and cirrhosis etiology were compared between patients receiving PPIs and those that did not.Furthermore,the rebleeding interval was compared between patients that received PPI treatment after EVO and those who did not.RESULTS:The patient group included nine males and seven females with a mean age of 61.8±11.7years.Following the EVO procedure,eight of the 12patients that received PPIs and three of the four nonPPI patients experienced rebleeding.There were no differences between the groups in serum biochemistry or patient characteristics.The rebleeding rate was not significantly different between the groups,however,patients receiving PPIs had a significantly longer rebleeding interval compared to non-PPI patients(22.2±11.2mo vs 8.5±5.5 mo;P=0.008).The duration of PPI use was not related to the rebleeding interval.A total of six patients,who had ulcers at the injection site,exhibited a shorter rebleeding interval(16.8±5.9 mo)than patients without ulcers(19.9±3.2 mo),though this difference was not statistically significant.CONCLUSION:PPI therapy can extend the rebleeding interval,and should therefore be considered after EVO treatment for gastric varices. | Won Seok Jang Hyun Phil Shin Joung Ⅱ Lee Kwang Ro Joo Jae Myung Cha Jung Won Jeon Jun Uk Lim | 2014 | World Journal of Gastroenterology2014，20，45： | 17 |
| 4 | Diffusion-weighted imaging of biliopancreatic disorders:Correlation with conventional magnetic resonance imaging显示文摘Diffusion-weighted magnetic resonance imaging(DWI)is a well established method for the evaluation of intracranial diseases,such as acute stroke.DWI for extracranial application is more difficult due to physiological motion artifacts and the heterogeneous composition of the organs.However,thanks to the newer technical development of DWI,DWI has become increasingly used over the past few years in extracranial organs including the abdomen and pelvis.Most previous studies of DWI have been limited to the evaluation of diffuse parenchymal abnormalities and focal lesions in abdominal organs,whereas there are few studies about DWI for the evaluation of the biliopancreatic tract.Although further studies are needed to determine its performance in evaluating bile duct,gallbladder and pancreas diseases,DWI has potential in the assessment of the functional information on the biliopancreatic tract concerning the status of tissue cellularity,because increased cellularity is associated with impeded diffusion,as indicated by a reduction in the apparent diffusion coefficient.The detection of malignant lesions and their differentiation from benign tumor-like lesions in the biliopancreatic tract could be improved using DWI in conjunction with findings obtained with conventional magnetic resonance cholagiopancreatography.Additionally,DWI can be useful for the assessment of the biliopancreatic tract in patients with renal impairment because contrast-enhanced computed tomography or magnetic resonance scans should be avoided in these patients. | Nam Kyung Lee Suk Kim Gwang Ha Kim Dong Uk Kim Hyung Il Seo Tae Un Kim Dae Hwan Kang Ho Jin Jang | 2012 | World Journal of Gastroenterology2012，18，31： | 8 |
| 5 | Survival analysis following early surgical success in intermittent exotropia surgery显示文摘AIM:To assess the consecutive recurrence following early success of intermittent exotropia surgery and to determine the clinical factors that affect the survival.METHODS:One hundred and thirty-five patients who underwent intermittent exotropia surgery and experienced early surgical success[≤5 prism diopters(PD)esophoria(E)to≤10 PD exophoria(X)on the postoperative sixth month]were enrolled in this study.Their consecutive survival on the postoperative first year,second year and third year and at the last visit of fourth year or more,and the factors that might affect their survival,were analyzed.The final surgical outcomes after the postoperative fourth year were also investigated by dividing the patients into the success group(≤5 PD E to≤10 PD X)and the failure group(>5 PD esodeviation or>10 PD exodeviation)RESULTS:The survival rates from the Kaplan-Meier analysis were 97.78%,92.89%,83.70%and 50.49%on the postoperative first,second and third years and fourth year or more,respectively.None of the clinical factors was determined to have affected the survival.The amount of the exodrift was largest(2.29 PD)between the first year and the second year,and smallest(1.47 PD)between the fourth year and the last visit.Sixty-three patients had their final visit after the postoperative fourth year,and 29 of them were in the failure group.Twentyfive patients in the failure group had an intermittent exotropia(IXT)of<20 PD with good to fair distant fusion;two had an IXT of<20 PD with poor distant fusion;one had an IXT of≥20 PD with fair distant fusion;and another had delayed-onset consecutive esotropia.The exodeviation on the postoperative sixth month was smaller in the success group than in the failure group(2.81 PD vs 5.86 PD,P=0.012).The reoperation rate for recurrent IXT was 3.7%.CONCLUSION:The survival rate steadily decreases with the exodrift,but the amount of the exodrift decreases with long-term follow-up.The final outcomes demonstrate favorable results via surgical success or small-angle IXT with good fusion in most of the patients.A smaller deviation on the postoperative sixth month is associated with long-term survival. | Joo Yeon Lee Sung Ju Ko Sung Uk Baek | 2014 | International Journal of Ophthalmology(English edition)2014，7，3： | 8 |
| 6 | Discrepancies between primary physician practice and treatment guidelines for Helicobacterpylori infection in Korea显示文摘瞄准:在 Helicobacter pylori 的诊断和治疗评估主要照顾医生的态度(H。pylori ) 感染。方法:在汉城大城市的区域的主要照顾医生从 1 月回答了自我管理的问询表到 2003 年 3 月。结果:108 位医生对问询表作出回应。为严峻的 H 的最经常的原因。pylori 感染是胃的并且十二指肠溃疡(93.5% 和 88.9% ,分别地) 。为有 H 的病人。pylori 积极消化不良, 28.7% 医生总是试着根除蠕虫并且 34.4% 有选择地对待。主要照顾医生的一个大比例(28.7%) 对待 H。一个病人的请求基础上的 pylori。仅仅, 9.3% 主要照顾医生总是进行了在对待 H 以后测试的后续。pylori 感染。什么时候 H。pylori 没被第一治疗清除, 40.7% 医生再使用一样的政体, 16.7% 改变了到另一三倍的政体并且 25% 到四倍的政体。结论:指南的发行几乎独自没在实践上有很少影响,这很好被记录了。在在连续医药教育形式的主要照顾医生和胃的肠学之间的通讯被要求。 | Byeong Gwan Kim Ji Won Kim Ji Bong Jeong Young Jin Jung Kook Lae Lee Young Soo Park Jin Huk Hwang Jin Uk Kim Na Young Kim Dong Ho Lee Hyun Chae Jung In Sung Song | 2006 | World Journal of Gastroenterology2006，12，1： | 8 |
| 7 | Prospective evaluation of a treatment algorithm with enhanced guidewire manipulation protocol for EUS-guided biliary drainage after failed ERCP (with video)显示文摘 | Do Hyun Park Seung Uk Jeong Byung Uk Lee Sang Soo Lee Dong-Wan Seo Sung Koo Lee Myung-Hwan Kim | 2013 | Gastrointestinal Endoscopy2013，，1： | 7 |
| 8 | Long-term outcomes of endoscopic submucosal dissection for early gastric cancer: a single-center experience显示文摘 | Mun Ki Choi Gwang Ha Kim Do Youn Park Geun Am Song Dong Uk Kim Dong Yup Ryu Bong Eun Lee Jae Hoon Cheong Mong Cho | 2013 | Surgical Endoscopy2013，，11： | 6 |
| 9 | Double layered self-expanding metal stents for malignant esophageal obstruction, especially across the gastroesophageal junction显示文摘AIM: To evaluate the clinical outcomes of double-layered self-expanding metal stents (SEMS) for treatment of malignant esophageal obstruction according to whether SEMS crosses the gastroesophageal junction (GEJ). METHODS: Forty eight patients who underwent the SEMS insertion for malignant esophageal obstruction were enrolled. Patients were classified as GEJ group (SEMS across GEJ, 18 patients) and non-GEJ group (SEMS above GEJ, 30 patients) according to SEMS position. Double layered (outer uncovered and inner covered stent) esophageal stents were placed. RESULTS: The SEMS insertion and the clinical improvement were achieved in all patients in both groups. Stent malfunction occurred in seven patients in the GEJ group and nine patients in the non-GEJ group. Tumor overgrowth occurred in five and eight patients, respectively, food impaction occurred in one patient in each group, and stent migration occurred in one and no patient, respectively. There were no significant differences between the two groups. Reflux esophagitis occurred more frequently in the GEJ group (eight vs five patients, P = 0.036) and was controlled by proton pump inhibitor. Aspiration pneumonia occurred in zero and five patients, respectively, and tracheoesophageal fistula occurred in zero and two patients, respectively. CONCLUSION: Double-layered SEMS are a feasible and effective treatment when placed across the GEJ for malignant esophageal obstruction. Double-layered SEMS provide acceptable complications, especially migration, although reflux esophagitis is more common in the GEJ group. | Min Dae Kim Su Bum Park Dae Hwan Kang Jae Hyung Lee Cheol Woong Choi Hyung Wook Kim Chung Uk Chung Young Il Jeong | 2012 | World Journal of Gastroenterology2012，18，28： | 5 |
| 10 | Comparison of gemcitabine plus nab-paclitaxel and FOLFIRINOX in metastatic pancreatic cancer显示文摘BACKGROUND Gemcitabine plus nab-paclitaxel(GA)and modified FOLFIRINOX(FFX)have been widely used as standard first-line treatment in pancreatic cancer.However,it is unclear which regimen is more efficacious.AIM To evaluate a retrospective analysis comparing the efficacy and safety of FFX and GA as first-line chemotherapeutic regimens in patients with metastatic pancreatic cancer.METHODS We retrospectively analyzed and compared outcomes in 101 patients who presented with pancreatic cancer and were treated with either GA(n=54)or FFX(n=47).Moreover,we performed subgroup analysis based on the neutrophil/lymphocyte ratio(NLR)and Eastern Cooperative Oncology Group(ECOG)performance status.RESULTS There were no significant differences between two groups in baseline characteristics,except for the ECOG performance status.The median progression-free survival(PFS)(6.43 mo vs 4.90 mo,P=0.058)was comparable between two groups;however,median overall survival(OS)(10.17 mo vs 6.93 mo,P=0.008)was longer in patients who received GA regimen.In patients with ECOG 0(PFS:8.93 mo vs 5.43 mo,P=0.002;OS:16.10 mo vs 6.97 mo,P=0.000)and those with NLR<3(PFS:8.10 mo vs 6.57 mo,P=0.008;OS:12.87 mo vs 9.93 mo,P=0.002),GA regimen showed higher efficacy.CONCLUSION GA regimen may be recommended to the patients with NLR<3 or ECOG 0 status although GA and FFX showed comparable efficacy outcomes in patients with metastatic pancreatic cancer. | Sung Yong Han Dong Uk Kim Young Mi Seol Suk Kim Nam Kyung Lee Seung Baek Hong Hyung-Il Seo | 2020 | World Journal of Clinical Cases2020，8，17： | 5 |
| 11 | Repeat hepatic resection in patients with colorectal liver metastases显示文摘AIM:To investigate the survival outcomes of secondaryhepatectomy for recurrent colorectal liver metastases(CRLM).METHODS:From October 1994 to December 2009,patients with CRLM who underwent surgical treatment with curative intent were investigated.Patients were divided into two groups:patients who underwent primary hepatectomy(Group 1)and those who underwent secondary hepatectomy for recurrent CRLM(Group 2).RESULTS:Survival and prognostic factors were analyzed.A total of 461 patients were included:406patients in Group 1 and 55 patients in Group 2.After a median 39-mo(range,3-195 mo)follow-up,there was a significant difference between Groups 1 and 2in terms of disease-free survival(P=0.029)although there was no significant difference in overall survival(P=0.206).Secondary hepatectomy was less effective in patients with multiple recurrent CRLM than primary hepatectomy for initial CRLM(P=0.008).Multiple CRLM and radiofrequency ablation therapy were poor prognostic factors of secondary hepatectomy in multivariate Cox regression analysis(P=0.006,P=0.004,respectively).CONCLUSION:Secondary hepatectomy for single recurrent CRLM is as effective as primary surgical treatment for single recurrent CRLM.However,secondary hepatectomy for multiple recurrent CRLM is less effective than that for single recurrent CRLM. | Huisong Lee Seong Ho Choi Yong Beom Cho Seong Hyeon Yun Hee Cheol Kim Woo Yong Lee Jin Seok Heo Dong Wook Choi Kyung Uk Jung Ho-Kyung Chun | 2015 | World Journal of Gastroenterology2015，21，7： | 4 |
| 12 | Avoiding heating interference and guided thermal conduction in stretchable devices using thermal conductive composite islands显示文摘The miniaturization and high integration of devices demand significant thermal management materials.Current technologies for the thermal management of electronics show some limitations in the case of multiple chip arrays.A device in multiple chip array is affected by heat from adjacent devices,along with thermal conductive composite.To address this problem,we present a nano composite of aligned boron nitride(BN)nanosheet islands with porous polydimethylsiloxane(PDMS)foam to have mechanical stability and non-thermal interference.The islands of tetrahedrally-structured BN in the composite have a high thermal conductivity of 1.219 W·m^(-1)·K^(-1) in the through-plane direction(11.234W·m^(-1)·K^(-1)in the in-plane direction)with 16 wt.%loading of BN.On the other hand,porous PDMS foam has a low thermal conductivity of 0.0328W·m^(-1)·K^(-1) in the through-plane direction at 70%porosity.Heat pathways are then formed only in the structured BN islands of the composite.The porous PDMS foam can be applied as a thermal barrier between structured BN islands to inhibit thermal interference in multiple device arrays.Furthermore,this composite can maintain selective thermal dissipation performance with 70%tensile strain.Another beauty of the work is that it could have guided heat dissipation by assembling of multiple layers which have high vertical thermal conductive islands,while inhibiting thermal interference.The selective heat dissipating composite can be applied as a heatsink for multiple chip arrays electronics. | Seung Ji Kang Haeleen Hong Chanho Jeong Ju Seung Lee Hyewon Ryu Jae-hun Yang Jong Uk Kim Yiel Jae Shin Tae-il Kim | 2021 | Nano Research2021，14，9： | 4 |
| 13 | Is ineffective esophageal motility associated with gastropharyngeal reflux disease?显示文摘AIM: To evaluate the association between ineffective esophageal motility (IEM) and gastropharyngeal reflux disease (GPRD) in patients who underwent ambulatory 24-h dual-probe pH monitoring for the evaluation of supraesophageal symptoms. METHODS: A total of 632 patients who underwent endoscopy,esophageal manometry and ambulatory 24-h dual-pH monitoring due to supraesophageal symptoms (e.g. globus,hoarseness,or cough) were enrolled. Of them,we selected the patients who had normal esophageal motility and IEM. The endoscopy and ambulatory pH monitoring findings were compared between the two groups. RESULTS: A total of 264 patients with normal esophageal motility and 195 patients with the diagnosis of IEM were included in this study. There was no difference in the frequency of reflux esophagitis and hiatal hernia between the two groups. All the variables showing gastroesophageal reflux and gastropharyngeal reflux were not different between the two groups. The frequency of GERD and GPRD,as defined by ambulatory pH monitoring,was not different between the two groups. CONCLUSION: There was no association between IEM and GPRD as well as between IEM and GERD. IEM alone cannot be considered as a definitive marker for reflux disease. | Kyung Yup Kim Gwang Ha Kim Dong Uk Kim Soo Geun Wang Byung Joo Lee Jin Choon Lee Do Youn Park Geun Am Song | 2008 | World Journal of Gastroenterology2008，14，39： | 3 |
| 14 | A revised scoring system utilizing serum alphafetoprotein levels to expand candidates for living donor transplantation in hepatocellular carcinoma显示文摘 | Sung Hoon Yang Kyung-Suk Suh Hae Won Lee Eung-Ho Cho Jai Young Cho Yong Beom Cho In Hwan Kim Nam-Joon Yi Kuhn Uk Lee | 2007 | Surgery2007，，5： | 3 |
| 15 | Acute distal common bile duct angle is risk factor for postendoscopic retrograde cholangiopancreatography pancreatitis in beginner endoscopist显示文摘BACKGROUND Post-endoscopic retrograde cholangiopancreatography(ERCP)pancreatitis(PEP)is a critical and poorly managed complication of ERCP.Endoscopists need to understand the risk factors for PEP.However,the majority of studies investigating ERCP-related risk factors have included well-trained endoscopists,with the issue of endoscopist experience on PEP incidence not having been systematically evaluated.AIM To explore the risk factors for PEP in beginner endoscopists without supervision.METHODS We performed a retrospective analysis of 293 patients,with naïve papilla and no history of pancreatitis,treated using bile duct cannulation.Patients were classified according to the endoscopist’s experience(beginner vs expert).The angle of the distal common bile duct(CBD)was measured as the angle between the lower wall of the bile duct and a vertical line extending to the lower wall of the bile duct on coronal view computed tomography.RESULTS After propensity matching,there were no differences between patients treated by the expert and beginner endoscopist with regard to age,sex,mean bile duct dilatation,and ratio of benign disease.The distal CBD angle was classified as acute(>30º)or obtuse(≤30º),based on the mean angle of 29.9ºfor the group.An acute distal CBD angle was a significant risk factor for PEP for beginner(P=0.049),but not expert.CONCLUSION For beginner endoscopists first performing unsupervised ERCP,cases with an obtuse distal CBD angle may be more appropriate to lower the risk of PEP. | Sung Yong Han Dong Uk Kim Moon Won Lee Young Joo Park Dong Hoon Baek Gwang Ha Kim Geun Am Song | 2020 | World Journal of Clinical Cases2020，8，1： | 3 |
| 16 | Prostate-specific antigen density predicts favorable pathology and biochemical recurrence in patients with intermediate-risk prostate cancer显示文摘这研究被设计与中间风险的前列腺癌症(IRPCa ) 在病人识别有利病理和生物化学的复发(BCR ) 的临床的预言者。在 2006 和 2012 之间,从为 IRPCa 经历帮助机器人的激进的前列腺切除术(RARP ) 的 203 个连续的人的 clinicopathological 和 oncological 数据在单人赛机构的回顾的研究被考察。有利病理被定义为格利森分数 ≤ 6 并且由外科的病理检测了的限制器官的癌症。逻辑回归分析被用来决定有利病理的预兆的变量,并且 Kaplan-Meier 和 multivariate 考克斯回归模型被用来在 RARP 以后估计 BCR 免费的幸存。总的来说, 38 个病人(18.7%) 在 RARP 以后有有利病理。更低方照前列腺特定的抗原密度(PSAD ) 与有利病理被联系与相比最高方照 PSAD 在为外科手术前的 PSA 调整以后,临床的舞台和活体检视格利森得分(机会比率, 5.42;95% 信心间隔, 1.01-28.97;P = 0.048 ) 。在期间一中部 37.8 (interquartile 范围, 24.6-60.2 ) 后续的月, 66 个病人经历了 BCR。关于由 PSAD quartiles 的 BCR 免费幸存有重要差别(木头等级, P = 0.003 ) 。用一个 multivariable 考克斯比例危险模型, PSAD 被发现是在有在 RARP 以后的 IRPCa 的病人的 BCR 的一个独立预言者(危险比率, 4.641;95% 信心间隔, 1.109-19.417;P = 0.036 ) 。进风险评价的 PSAD 的加入可能提供另外的预示的信息并且识别活跃监视将在有 IRPCa 的病人是谁适当的一些病人。 | Ho Won Kang Hae Do Jung Joo Yong Lee Jong Kyou Kwon Seong Uk Jeh Kang Su Cho Won Sik Ham Young Deuk Choi | 2016 | Asian Journal of Andrology2016，18，3： | 3 |
| 17 | Negative oncologic impact of poor postoperative pain control in left-sided pancreatic cancer显示文摘AIM To investigate the association between postoperative pain control and oncologic outcomes in resected pancreatic ductal adenocarcinoma(PDAC).METHODS From January 2009 to December 2014, 221 patients were diagnosed with PDAC and underwent resection with curative intent. Retrospective review of the patients was performed based on electronic medical records system. One patient without records of numerical rating scale(NRS) pain intensity scores was excluded and eight patients who underwent total pancreatectomy were also excluded. NRS scores during 7 postoperative days following resection of PDAC werereviewed along with clinicopathologic characteristics. Patients were stratified into a good pain control group and a poor pain control group according to the difference in average pain intensity between the early(POD 1, 2, 3) and late(POD 5, 7) postoperative periods. Cox-proportional hazards multivariate analysis was performed to determine association between postoperative pain control and oncologic outcomes.RESULTS A total of 212 patients were dichotomized into good pain control group(n = 162) and poor pain control group(n = 66). Median follow-up period was 17 mo. A negative impact of poor postoperative pain control on overall survival(OS) was observed in the group of patients receiving distal pancreatectomy(DP group; 42.0 mo vs 5.0 mo, P = 0.001). Poor postoperative pain control was also associated with poor disease-free survival(DFS) in the DP group(18.0 mo vs 8.0 mo, P = 0.001). Patients undergoing pancreaticoduodenectomy or pylorus-preserving pancreaticoduodenectomy(PD group) did not show associations between postoperative pain control and oncologic outcomes. Poor patients' perceived pain control was revealed as an independent risk factor of both DFS(HR = 4.157; 95%CI: 1.938-8.915; P < 0.001) and OS(HR = 4.741; 95%CI: 2.214-10.153; P < 0.001) in resected left-sided pancreatic cancer.CONCLUSION Adequate postoperative pain relief during the early postoperative period has important clinical implications for oncologic outcomes after resection of left-sided pancreatic cancer. | Eun-Ki Min Jae Uk Chong Ho Kyoung Hwang Sang Joon Pae Chang Moo Kang Woo Jung Lee | 2017 | World Journal of Gastroenterology2017，23，4： | 2 |
| 18 | Risk Factors for Operative Complications in Elderly Patients During Laparoscopy-Assisted Gastrectomy显示文摘 | Sun-Hwi Hwang Do Joong Park Ye Seob Jee Hyung-Ho Kim Hyuk-Joon Lee Han-Kwang Yang Kuhn Uk Lee | 2009 | Journal of the American College of Surgeons2009，，2： | 2 |
| 19 | Laparoscopic-Assisted Versus Open Complete Mesocolic Excision and Central Vascular Ligation for Right-Sided Colon Cancer显示文摘 | Sung Uk Bae Avanish P. Saklani Dae Ro Lim Dong Wook Kim Hyuk Hur Byung Soh Min Seung Hyuk Baik Kang Young Lee Nam Kyu Kim | 2014 | Annals of Surgical Oncology2014，，7： | 2 |
| 20 | GnRH impairs diabetic wound healing through enhanced NETosis显示文摘It has been reported that neutrophil extracellular traps(NETs)impair wound healing in diabetes and that inhibiting NET generation(NETosis)improves wound healing in diabetic mice.Gonadotropin-releasing hormone(GnRH)agonists are associated with a greater risk of diabetes.However,the role of GnRH in diabetic wound healing is unclear.We determined whether GnRH-promoted NETosis and induced more severe and delayed diabetic wound healing.A mouse model of diabetes was established using five injections with streptozotocin.Mice with blood glucose levels>250 mg/dL were then used in the experiments.GnRH agonist treatment induced delayed wound healing and increased NETosis at the skin wounds of diabetic mice.In contrast,GnRH antagonist treatment inhibited GnRH agonist-induced delayed wound healing.The expression of NETosis markers PAD4 and citrullinated histone H3 were increased in the GnRH-treated diabetic skin wounds in diabetic mice and patients.In vitro experiments also showed that neutrophils expressed a GnRH receptor and that GnRH agonist treatment increased NETosis markers and promoted phorbol myristate acetate(PMA)-induced NETosis in mouse and human neutrophils.Furthermore,GnRH antagonist treatment suppressed the expression of NETosis markers and PMA-induced NETosis,which were increased by GnRH treatment.These results indicated that GnRH-promoted NETosis and that increased NETosis induced delayed wound healing in diabetic skin wounds.Thus,inhibition of GnRH might be a novel treatment of diabetic foot ulcers. | Yun Sang Lee Sung Un Kang Myung-Hoon Lee Haeng-Jun Kim Chang-Hak Han Ho-Ryun Won Young Uk Park Chul-Ho Kim | 2020 | Cellular & Molecular Immunology2020，17，8： | 2 |
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