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| 1 | Transactivation of the TIEG1 confers growth inhibition of transforming growth factor-β-susceptible hepatocellular carcinoma cells显示文摘AIM:To investigate the role of transforming growth factor(TGF)-β-inducible early gene 1(TIEG1) in TGF-β-induced growth inhibition in hepatocellular carcinoma(HCC) cells.METHODS:Human hepatocyte and HCC cell lines with varied susceptibilities to TGF-β1 were tested by methylthiazoletetrazolium(MTT) assay.The expression changes of Smad2,Smad3,Smad4,Smad7,TIEG1 and TIEG2 gene following treatment with TGF-β1 in a TGF-β-sensitive hepatocyte cell line(MIHA),a TGF-β-sensitive hepatoma cell line(Hep3B) and two TGF-β-insensitive hepatoma cell lines(HepG2 and Bel7404) were examined.SiRNA targeting TIEG1 was transfected into Hep3B cells and the sensitivity of cells to TGF-β1 was examined.Overexpression of TIEG1 was induced by lentiviral-mediated transduction in TGF-β1-resistant hepatoma cell lines(Bel7404 and HepG2).MTT assay and 4',6-Diamidino-2-phenylindole staining were used to identify cell viability and apoptosis,respectively.The expression level of stathmin was measured by reverse transcriptase polymerase chain reaction and Western-blotting analysis,and stathmin promoter activity by TIEG1 was monitored by a luciferase reporter gene system.RESULTS:TIEG1 was significantly upregulated by TGF-β1 in the TGF-β1-sensitive HCC cell line,Hep3B,but not in the resistant cell lines.The suppression of TIEG1 by siRNAs decreased the sensitivity of Hep3B cells to TGF-β1,whereas the overexpression of TIEG1 mediated growth inhibition and apoptosis in TGF-β1-resistant HCC cell lines,which resembled those of TGF-β1-sensitive HCC cells treated with TGF-β1.Our data further suggested that stathmin was a direct target of TIEG1,as stathmin was signif icantly downregulated by TIEG1 overexpression,and stathmin promoter activity was inhibited by TIEG1 in a dose-dependent manner.CONCLUSION:Our data suggest that transactivation of TIEG1 conferred growth inhibition of TGF-β-susceptible human HCC cells. | Lei Jiang Yiu-Kay Lai Jin-Fang Zhang Chu-Yan Chan Gang Lu Marie CM Lin Ming-Liang He Ji-Cheng Li Hsiang-Fu Kung | 2012 | World Journal of Gastroenterology2012，18，17： | 13 |
| 2 | Regulation of roTOR and cell growth in response to energy stress by REDDI显示文摘 | Sofer A Lei K Johannessen CM | 2005 | Mol Cell Biol2005，25，14： | 1 |
| 3 | Expression and prognos- tic significance of prothymosin-alpha and ERp57 in human gas- tric cancer 显示文摘 | Leys CM Nomura S LaFleur BJ | 2007 | Surgery2007，141，1： | 1 |
| 4 | A complete course of intravenous antibiotics vs a combination of intravenous and oral antibiotics for perforated appendicitis in children: a prospective, randomized trial 显示文摘 | Fraser JD Aguayo P Leys CM | 2010 | J Pediatr Surg2010，45，6： | 1 |
| 5 | A complete course ofintravenous antibiotics vs a combination of intravenous and oralantibiotics for perforated appendicitis in children: a prospective,randomized trial显示文摘 | Fraser JD Aguayo P Leys CM | 2010 | J Pediatr Surg2010，45，6： | 1 |
| 6 | 在西班牙儿童中高能量高盐食物摄入频率与舒张期高血压相关显示文摘高血压是心血管病的危险因素,而钠摄入与高血压相关。此外,含糖饮料及终止高血压膳食疗法(dietary approach to stop hypertension,DASH)也影响血压。基于以上原因,本研究旨在探究:(1)有血压升高风险的儿童是否有较高的高能量高盐食物(energydense salty foods,EDSF)、高含糖量食物(high-sugary foods)和含糖饮料摄入频率或者较低的DASH评分;(2)EDSF摄入频率较高的儿童人体测量学和代谢情况是否较差。 | 袁源(摘译) 练桂丽(审校) Pérez-Gimeno G Rupérez AI Vázquez-Cobela R Herráiz-Gastesi G Gil-Campos M Aguilera CM Moreno LA Leis Trabazo MR Bueno-Lozano G | 2020 | 中华高血压杂志2020，28，11： | 1 |
| 7 | Liver transplantation for childhood hepatic malignancy:a review of the United Network for Organ Sharing (UNOS) database显示文摘 | Austin MT Leys CM Feurer ID | | 0，，1： | 1 |
| 8 | PYY[3-36]administration decreases the respiratory quotient and reduces adiposity in diet-induced obese nice显示文摘 | ADAMS SH LEI C JODKA CM et at | | 0，，： | 1 |
| 9 | Elective intestinal operations in infants and children without mechanical bowel preparation:a pilot study显示文摘 | Leys CM Austin MT Pietsch JB | 2005 | J Pediatr Surg2005，40，6： | 1 |
| 10 | Regulation of m TOR and cell growth in response to energy stress by REDD1显示文摘 | Sofer A Lei K Johannessen CM | 2005 | Mol Cell Biol2005，25，14： | 1 |
| 11 | Liver transplantation for childhood hepatic malignancy: a review of the United Network for Organ Sharing (UNOS) database 显示文摘 | Austin MT Leys CM Feurer ID | 2006 | J Pediatr Surg2006，41，1： | 1 |
| 12 | Expression and prognosticsignificance of prothymosin-alpha and ERp57 in human gastriccancer显示文摘 | Leys CM Nomura S LaFleur BJ | 2007 | Surgery2007，141，1： | 1 |
| 13 | Soybean WRKY-type transcription factor genes, GmWRKY13, GmWRKY21, and GmWRKY54, confer differential tolerance to abiotic stresses in transgenic Arabidopsis plants 显示文摘 | Zhou Q Y Tian A G Zou H F Xie Z M Lei G Huang J Wang CM Wang H W Zhang J S Chen S Y | 2008 | Plant Biotechnology Journal2008，6，5： | 1 |
| 14 | Mild cognitive impairment : risk of dementia according to subtypes 显示文摘 | Serrano CM Dillon C Leis A | 2013 | Actas Esp Psiquiatr2013，41，6： | 1 |
| 15 | Laparoscopic removal of a gastrictrichobezoar in a pediatric patient显示文摘 | Fraser J D Leys CM St Peter S D | 2009 | J Laparoendosc Adv Surg Tech A2009，19，10： | 1 |
| 16 | Expression and prognosticsignificance of prothymosin-alpha and ERp57 in human gastricCancer显示文摘 | Leys CM Nomura S Lafleur BJ | 2007 | Surgery2007，141，1： | 1 |
| 17 | Surgical Resection of Gastrointestinal Stromal Tumors After Treatment with Imatinib显示文摘 | Robert H. I. Andtbacka MD CM Chaan S. Ng MD Courtney L. Scaife MD Janice N. Cormier MD MPH Kelly K. Hunt MD Peter W. T. Pisters MD Raphael E. Pollock MD PhD Robert S. Benjamin MD Michael A. Burgess MD Lei L. Chen MD PhD Jonathan Trent MD Shreyaskumar | 2007 | Annals of Surgical Oncology2007，，1： | 1 |
| 18 | Using a Peat Biobarrier to Remediate TCE Contaminated Aquifer显示文摘 | Lei S E | 2000 | Waters2000，34，3： | 1 |
| 19 | Prophylactic antibiotics forprevention of cholangitis in patients with biliary atresia status post-Kasai portoenterostomy:a systematic review显示文摘 | Decharun K Leys CM West KW | 2016 | Clin Pediatr(Phila)2016，55，1： | 1 |
| 20 | Surgical Resection of Gastrointestinal Stromal Tumors After Treatment with Imatinib显示文摘 | Robert H. I. Andtbacka MD CM Chaan S. Ng MD Courtney L. Scaife MD Janice N. Cormier MD MPH Kelly K. Hunt MD Peter W. T. Pisters MD Raphael E. Pollock MD PhD Robert S. Benjamin MD Michael A. Burgess MD Lei L. Chen MD PhD Jonathan Trent MD Shreyaskumar | 2007 | Annals of Surgical Oncology2007，，1： | 1 |
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