|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The chromosome-level reference genome assembly for Panax notoginseng and insights into ginsenoside biosynthesis显示文摘Panax notoginseng,a perennial herb of the genus Panax in the family Araliaceae,has played an important role in clinical treatment in China for thousands of years because of its extensive pharmacological effects.Here,we report a high-quality reference genome of P.notoginseng,with a genome size up to 2.66 Gb and a contig N50 of 1.12 Mb,produced with third-generation PacBio sequencing technology.This is the first chromosome-level genome assembly for the genus Panax.Through genome evolution analysis,we explored phylogenetic and whole-genome duplication events and examined their impact on saponin biosynthesis.We performed a detailed transcriptional analysis of P.notoginseng and explored genelevel mechanisms that regulate the formation of characteristic tubercles.Next,we studied the biosynthesis and regulation of saponins at temporal and spatial levels.We combined multi-omics data to identify genes that encode key enzymes in the P.notoginseng terpenoid biosynthetic pathway.Finally,we identified five glycosyltransferase genes whose products catalyzed the formation of different ginsenosides in P.notoginseng.The genetic information obtained in this study provides a resource for further exploration of the growth characteristics,cultivation,breeding,and saponin biosynthesis of P.notoginseng. | Zhouqian Jiang Lichan Tu Weifei Yang Yifeng Zhang Tianyuan Hu Baowei Ma Yun Lu Xiuming Cui Jie Gao Xiaoyi Wu Yuru Tong Jiawei Zhou Yadi Song Yuan Liu Nan Liu Luqi Huang Wei Gao | 2021 | Plant Communications2021，2，1： | 11 |
| 2 | Characterization of codon usage bias in the dUTPase gene of duck enteritis virus显示文摘A comparative analysis of the codon usage bias in the newly discovered dUTPase gene (Assigned Accession No.:DQ486149) of the duck enteritis virus (DEV) and the dUTPase gene of 32 reference herpesviruses was performed.The results indicated that the DEV dUT-Pase gene encodes a protein of 477 amino acids, which includes five conserved motifs with a 3-1-2-4-5 arrangement.The codon adap-tation index (CAI), efiective number of codons (ENC), and GC3S values indicated synonymous codon usage bias in the dUTPase gene of herpesviruses, and this synonymous bias was correlated with host evolution.The codon usage patterns of the DEV dUTPase gene were phylogenetically conserved and similar to that of the dUTPase genes of the avian alphaherpesvirus.Although codon usage in each micro-organism was difierent, there were no strain-specific difierences among them.Sixty-one codons in the predicted polypeptide, with a strong bias towards A and T at the third codon position, were used.Comparison of the codon usage in the dUTPase gene of difierent organisms revealed that there were 19 codons showing distinct codon usage difierences between the DEV and Escherichia coli dUTPase genes;16 between the DEV and yeast dUTPase genes;and 15 between the DEV and human dUTPase genes.Analysis of variance (ANOVA) showed significant difierences between the DEV and yeast dUTPase genes (r = 0.536, P < 0.01).The extent of codon usage bias in the DEV dUTPase gene was highly correlated with the gene expression level, therefore the results may provide useful information for gene classification and functional studies. | Lichan Zhao Anchun Cheng Mingshu Wang Guiping Yuan Mingsheng Cai | 2008 | Progress in Natural Science:Materials International2008，18，9： | 4 |
| 3 | Reconstruction and visualization of 3D models of colonic surface显示文摘 | Lichan Hong Zhengrong Liang | 1997 | IEEE Transaction on Nuclear Science1997，44，3： | 2 |
| 4 | Mechanistic analysis for the origin of diverse diterpenes in Tripterygium wilfordii显示文摘Tripterygium wilfordii is a valuable medicinal plant rich in biologically active diterpenoids,but there are few studies on the origins of these diterpenoids in its secondary metabolism.Here,we identified three regions containing tandemly duplicated diterpene synthase genes on chromosomes(Chr) 17 and 21 of T. wilfordii and obtained 11 diterpene synthases with different functions.We farther revealed that these diterpene synthases underwent duplication and rearrangement at approximately 2.3-23.7 million years ago(MYA) by whole-genome triplication(WGT),transposon mediation,and tandem duplication,followed by functional divergence.We first demonstrated that four key amino acids in the sequences of TwCPS3,TwCPS5,and TwCPSS were altered during evolution,leading to their functional divergence and the formation of diterpene secondary metabolites.Then,we demonstrated that the functional divergence of three TwKSLs was driven by mutations in two key amino acids.Finally,we discovered the mechanisms of evolution and pseudogenization of miltiradiene synthases in T.wilfordii and elucidated that the new function in TwMS1/2 from the terpene synthase(TPS)-b subfamily was caused by progressive changes in multiple amino acids after the WGT event.Our results provide key evidence for the formation of diverse diterpenoids during the evolution of secondary metabolites in T.wilfordii. | Lichan Tu Xinbo Cai Yifeng Zhang Yuru Tong Jian Wang Ping Su Yun Lu Tianyuan Hu Yunfeng Luo Xiaoyi Wu Dan Li Luqi Huang Wei Gao | 2022 | Acta Pharmaceutica Sinica B2022，12，6： | 1 |
| 5 | Stability of bovine immunoglobulins to thermal treatment and processing 显示文摘 | LiChan E Kummer A Losso J | 1995 | Food Research International1995，28，1： | 1 |
| 6 | Relationship between functional (fat binding, emulsifying) and physicochemical properties of muscle proteins-Effects of heating, freezing, pH and species 显示文摘 | Lichan E Nakai S Wood D F | 1985 | Journal of Food Science1985，50，4： | 1 |
| 7 | Stability of bovine immunoglobulins to thermal treatment and processing显示文摘 | Lichan E Kummer A Losso JN | 1995 | Food Research International1995，28，： | 1 |
| 8 | Homology similarity analysis of sequences of lactoferricin and its derivatives显示文摘 | Chan JC LiChan EC | 2003 | J Agric Food Chem2003，51，5： | 1 |
| 9 | Real Time Processing of Affective and Cognitive Stimuli in the Human Brain Extracted from MEG Signals显示文摘 | Andreas A. Ioannides Lichan Liu Dionyssios Theofilou Jürgen Dammers Tom Burne TimAmbler Ambler Steven Rose | 2000 | Brain Topography2000，，1： | 1 |
| 10 | Reliable Path for Virtual Endoscopy:Ensuring Complete Examination of Human Organs显示文摘 | Taosong He Lichan Hong Dongqing Chen and Zhengrong Liang | 2001 | IEEE Transactions on Visualization and Computer Graphics2001，7，4： | 1 |
| 11 | Sequencing and comparative analyses of Aegilops tauschii chromosome arm 3DS reveal rapid evolution of Triticeae genomes显示文摘Bread wheat(Triticum aestivum,AABBDD) is an allohexaploid species derived from two rounds of interspecific hybridizations.A high-quality genome sequence assembly of diploid Aegilops tauschii,the donor of the wheat D genome,will provide a useful platform to study polyploid wheat evolution.A combined approach of BAC pooling and next-generation sequencing technology was employed to sequence the minimum tiling path(MTP) of 3176 BAC clones from the short arm of Ae.tauschii chromosome 3(At3DS).The final assembly of 135 super-scaffolds with an N50 of 4.2 Mb was used to build a247-Mb pseudomolecule with a total of 2222 predicted protein-coding genes.Compared with the orthologous regions of rice,Brachypodium,and sorghum,At3 DS contains 38.67%more genes.In comparison to At3 DS,the short arm sequence of wheat chromosome 3B(Ta3BS) is 95-Mb large in size,which is primarily due to the expansion of the non-centromeric region,suggesting that transposable element(TE) bursts in Ta3 B likely occurred there.Also,the size increase is accompanied by a proportional increase in gene number in Ta3 BS.We found that in the sequence of short arm of wheat chromosome 3D(Ta3DS),there was only less than 0.27%gene loss compared to At3 DS.Our study reveals divergent evolution of grass genomes and provides new insights into sequence changes in the polyploid wheat genome. | Jingzhong Xie Naxin Huo Shenghui Zhou Yi Wang Guanghao Guo Karin R.Deal Shuhong Ouyang Yong Liang Zhenzhong Wang Lichan Xiao Tingting Zhu Tiezhu Hu Vijay Tiwari Jianwei Zhang Hongxia Li Zhongfu Ni Yingyin Yao Huiru Peng Shengli Zhang Olin D.Anderson Patrick E.McGuire Jan Dvorak Ming-Cheng Luo Zhiyong Liu Yong Q.Gu Qixin Sun | 2017 | Journal of Genetics and Genomics2017，44，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员