|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Increased leptin by hypoxic-preconditioning promotes autophagy of mesenchymal stem cells and protects them from apoptosis显示文摘Autophagy is the basic catabolic progress involved in cell degradation of unnecessary or dysfunctional cellular components.It has been proven that autophagy could be utilized for cell survival under stresses.Hypoxic-preconditioning(HPC)could reduce apoptosis induced by ischemia and hypoxia/serum deprivation(H/SD)in bone marrow-derived mesenchymal stem cells(BMSCs).Previous studies have shown that both leptin signaling and autophagy activation were involved in the protection against apoptosis induced by various stress,including ischemia-reperfusion.However,it has never been fully understood how leptin was involved in the protective effects conferred by autophagy.In the present study,we demonstrated that HPC can induce autophagy in BMSCs by increased LC3-II/LC3-I ratio and autophagosome formation.Interestingly,similar effects were also observed when BMSCs were pretreated with rapamycin.The beneficial effects offered by HPC were absent when BMSCs were incubated with autophagy inhibitor,3-methyladenine(3-MA).In addition,down-regulated leptin expression by leptin-shRNA also attenuated HPC-induced autophagy in BMSCs,which in turn was associated with increased apoptosis after exposed to sustained H/SD.Furthermore,increased AMP-activated protein kinase phosphorylation and decreased mammalian target of rapamycin phosphorylation that were observed in HPC-treated BMSCs can also be attenuated by down-regulation of leptin expression.Our data suggests that leptin has impact on HPC-induced autophagy in BMSCs which confers protection against apoptosis under H/SD,possibly through modulating both AMPK and mTOR pathway. | WANG LiHan HU XinYang ZHU Wei JIANG Zhi ZHOU Yu CHEN PanPan WANG JianAn | 2014 | Science China(Life Sciences)2014，57，2： | 8 |
| 2 | The rice Raf-like MAPKKK OsILA1 confers broad-spectrum resistance to bacterial blight by suppressing the OsMAPKK4-OsMAPK6 cascade显示文摘Mitogen-activated protein kinase kinase kinase(MAPKKK)are the first components of MAPK cascades,which play pivotal roles in signaling during plant development and physiological processes.The genome of rice encodes 75 MAPKKKs,of which 43 are Raf-like MAPKKKs.The functions and action modes of most of the Raf-like MAPKKKs,whether they function as bona fide MAPKKKs and which are their downstream MAPKKs,are largely unknown.Here,we identified the osmapkkk43 mutant,which conferred broad-spectrum resistance to Xanthomonas oryzae pv.oryzae(Xoo),the destructive bacterial pathogen of rice.Oryza sativa(Os)MAPKKK43 encoding a Raf-like MAPKKK was previously known as Increased Leaf Angle 1(OsILA1).Genetic analysis indicated that OsILA1 functioned as a negative regulator and acted upstream of the OsMAPKK4-OsMAPK6 cascade in rice-Xoo interactions.Unlike classical MAPKKKs,OsILA1 mainly phosphorylated the threonine 34 site at the N-terminal domain of OsMAPKK4,which possibly influenced the stability of OsMAPKK4.The N-terminal domain of OsILA1 is required for its homodimer formation and its full phosphorylation capacity.Taken together,our findings reveal that OsILA1 acts as a negative regulator of the OsMAPKK4-OsMAPK6 cascade and is involved in rice-Xoo interactions. | Jie Chen Lihan Wang Zeyu Yang Hongbo Liu Chuanliang Chu Zhenzhen Zhang Qinglu Zhang Xianghua Li Jinghua Xiao Shiping Wang Meng Yuan | 2021 | Journal of Integrative Plant Biology2021，63，10： | 3 |
| 3 | Impaired SWEET-mediated sugar transportation impacts starch metabolism in developing rice seeds显示文摘Sugar transportation and sugar-to-starch metabolism are considered important processes in seed development and embryo viability.A few plant SWEET proteins acting as sugar transporters have been reported to function in inflorescence and/or seed development.Here,we identified seven members of the 21 Os SWEET genes in rice that play essential roles in sugar transportation and sugar-to-starch conversion in seed development.Nineteen Os SWEET genes exhibiting different expression patterns during inflorescence and seed development were knocked out individually by CRISPR/Cas9.One third of the mutants showed decreased fertile pollen viability and shriveled mature caryopses,resulting in weakened seed traits.Grain fill-related genes but not representative grain shape-regulating genes showed attenuated expression in the mutants.Seed of each of these mutants accumulated more sucrose,glucose or fructose but less starch.Among all Os SWEET genes,Os SWEET4 and Os SWEET11 had major effects on caryopsis development.The sugar-to-starch metabolic pathway was significantly altered in ossweet11 mutants based on differential expression analysis in RNA sequencing assays,confirming that Os SWEET11 functions as a sugar transporter with a key role in seed development.These results help to decipher the multiple functions of Os SWEET genes and to show how they might be used in genetic improvement of rice. | Pei Li Lihan Wang Hongbo Liu Meng Yuan | 2022 | The Crop Journal2022，10，1： | 3 |
| 4 | Thermal stability of polyphenylsilsesquioxane-modified meta-aramid insulation paper显示文摘The development of new materials for use in advanced distribution transformers is required to improve their heat resistance rating,operational safety,and reliability.The combination of polymer and nanoparticles is the preferred approach to improve the thermal stability of materials.In this study,the thermal stability of laboratory-prepared meta-aramid insulation paper modified with polyphenylsilsesquioxane(PPSQ)was evaluated.The results showed that the thermal stability of the modified insulation paper was superior to that of the unmodified paper.Molecular dynamics simulations were used to analyse the internal mechanism of the performance improvement achieved by modification with PPSQ.From a microscopic perspective,the addition of PPSQ provided a relatively larger structural space for the meta-aramid fibre and increased the free volume of the entire system,which tightened the meta-aramid fibre molecular chains,increased the density of the structure of the paper,and enhanced the mutual permeability between meta-aramid fibre chains.Therefore,the stress can be better transmitted,the performance loss such as phase delamination was effectively prevented,and the bonding between the meta-aramid fibres was strengthened. | Chao Tang Wei Zheng Lihan Wang Jufang Xie | 2020 | High Voltage2020，5，3： | 1 |
| 5 | Kidney function change after transcatheter aortic valve replacement in patients with diabetes and/or hypertension显示文摘Aortic stenosis(AS)is a progressive heart valve disease occurring predominantly in older patients.According to a survey in a western country,the prevalence of AS is nearly 6.4%in patients over 75 years old(Carabello and Paulus,2009).Transcatheter aortic valve replacement(TAVR)is an alternative method for AS patients.Previous studies have described how up to 66%of TAVR patients have concomitant baseline kidney dysfunction(Ferro et al.,2015;Gargiulo et al.,2015).The majority of patients can benefit from the TAVR procedure with the recovery of kidney function. | Jiaqi FAN Changjie YU Kaida REN Wanbing LIN Stella NG Zexin CHEN Xinping LIN Lihan WANG Qifeng ZHU Yuxin HE Jubo JIANG Xianbao LIU Jian'an WANG | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021，22，3： | 1 |
| 6 | Novel apical-to-femoral rail technique for horizontal aorta in transcatheter aortic valve replacement显示文摘Transcatheter aortic valve replacement(TAVR)has emerged as a viable treatment option for patients with severe aortic stenosis regardless of its surgical risk stratification(Otto et al.,2021).Aortic angulation is usually measured as the angle between the horizon-tai and the aortic annulus planes based on preprocedural multidetector computed tomography(MDCT)(Al-Lamee et al.,2011).Extremely horizontal aorta,defined as an aortic angulation greater than 70°. | Xianbao LIU Hanyi DAI Lihan WANG Jiaqi FAN Jian’an WANG | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，7： | 0 |
| 7 | Research on Gesture Recognition Based on Multialgorithm Fusion显示文摘Aiming at people with hearing and speaking obstacles,this paper pro-poses a multialgorithm fusion for gesture recognition.This paper aims to more clearly distinguish easily confused gestures in gesture recognition and improve gesture recognition accuracy by integrating lip-reading recognition.For gesture recognition,this paperfirst performs skin color processing and segmentation on the hand area of the collected video sequence and detects the hand feature points by calling the hand key point model.The extracted gesture features are trained and recognized by the support vector machine algorithm.For lip reading recognition,this paperfirst uses the AdaBoost algorithm to detect and track key points on the collected video sequence,locate the lips,extract the key points of the lips through a convolutional neural network,and input the extracted key point feature sequence into BiLSTM to extract semantic information.The fusion of gesture recognition and lip reading recognition algorithms using the YOLOV5 model can effectively improve the accuracy of gesture recognition.Through experimental verification,the recognition rate can be increased from 89.4%to 94.3%. | Yuanyuan Zhu Yanqing Wang Xiaofeng Gao Lihan Liu | 2023 | 国际计算机前沿大会会议论文集2023，，2： | 0 |
| 8 | Nuclear events during conjugation in the poorly studied model ciliate Paramecium jenningsi显示文摘Ciliated protists are highly differentiated unicellular eukaryotes that possess special sexual processes(conjugation and autogamy)that rely on their unique nuclear dimorphism,i.e.,the presence of both a germline micronucleus(MIC)and a somatic macronucleus(MAC)in a single cell.The sexual processes show a high diversity in different ciliates.To better understand the differentiation and evolution of sexual processes in closely related species,we investigated the nuclear events during conjugation in Paramecium jenningsi strain GZ,a poorly studied close relative of the well-known P.aurelia complex.The main results include:(1)the conjugation process takes about 48 h,including three prezygotic divisions(meiosis I,II,and mitosis)and three postzygotic divisions;(2)the MICs are dominated by the“parachute”phase at the prophase of meiosis I;(3)after meiosis II,a variable number of nuclei undergo the third prezygotic division,and the two products near the paroral cone become the genetically identical migratory and stationary pronuclei;(4)the synkaryon divides three times to form the MIC and the MAC anlagen,but only one nuclear product of the first postzygotic division completes the next two divisions;and(5)an extra cell division is required to complete the last step of conjugation,during which two MIC anlagen undergo mitotic division while two MAC anlagen are distributed between the daughter cells without division.The comparison of the nuclear events during conjugation in representatives of the class Oligohymenophorea reveals that usually there are three prezygotic divisions and a variable number of postzygotic divisions.However,the number of selected and differentiated nuclei after each division differs among species.This study provided a new model ciliate for further investigations of nuclear selection and differentiation as well as nuclear morphology during meiosis. | Xue Zhang Ruitao Gong Yaohan Jiang Xiaoteng Lu Chenyin Wu Lihan Wang Hongwei Ma Zhengxiang Zhang Weibo Song Khaled A.S.Al-Rasheid Adriana Vallesi Feng Gao | 2023 | Water Biology and Security2023，2，4： | 0 |
| 9 | OsMAPK6 phosphorylates a zinc finger protein OsLIC to promote downstream OsWRKY30 for rice resistance to bacterial blight and leaf streak显示文摘Rice OsLIC encoding a CCCH zinc finger transcription factor plays an important role in immunity.However,the immune signaling pathways that OsLIC-involved and the underlying mechanisms that OsLIC-conferred resistance against pathogens are largely unclear.Here,we show that OsLIC,as a substrate for Os MAPK6,negatively regulates resistance to Xanthomonas oryzae pv.oryzae(Xoo)and X.oryzae pv.oryzicola(Xoc)by directly suppressing OsWRKY30 transcription.Biochemical assays showed that OsLIC bound to OsWRKY30 promoter and suppressed its transcription.Genetic assays confirmed that the osilc knockout mutants and OsWRKY30-overexpressing plants exhibited enhanced resistance to Xoo and Xoc,knocking out OsWRKY30 in the oslic mutants attenuated the resistance against bacterial pathogens.Os MAPK6 physically interacted with and phosphorylated OsLIC leading to decreased OsLIC DNA-binding activity,therefore,overexpression of OsLIC partially suppressed Os MAPK6-mediated rice resistance.In addition,both Os MAPK6-phosphorylated activation of OsLIC and phosphorylation-mimic OsLIC_(5D) had reduced DNA-binding activity towards OsWRKY30 promoter,thereby promoting OsWRKY30 transcription.Collectively,these results reveal that Os MAPK6-mediated phosphorylation of OsLIC positively regulates rice resistance to Xoo and Xoc by modulating OsWRKY30 transcription,suggesting that Os MAPK6-OsLIC-OsWRKY30 module is an immune signaling pathway in response to the bacterial pathogens. | Lihan Wang Jie Chen Yuqin Zhao Shiping Wang Meng Yuan | 2022 | Journal of Integrative Plant Biology2022，64，5： | 0 |
| 10 | Cerebral ischemic injury after transcatheter aortic valve replacement in patients with pure aortic regurgitation显示文摘Considering the surgical risk stratification for patients with severe calcific aortic stenosis(AS),transcatheter aortic valve replacement(TAVR)is a reliable alternative to surgical aortic valve replacement(SAVR)(Fan et al.,2020,2021;Lee et al.,2021).Despite the favorable clinical benefits of TAVR,stroke remains a dreaded perioperative complication(Auffret et al.,2016;Kapadia et al.,2016;Kleiman et al.,2016;Huded et al.,2019).Ischemic overt stroke,identified in 1.4%to 4.3%of patients in TAVR clinical practice,has been associated with prolonged disability and increased mortality(Auffret et al.,2016;Kapadia et al.,2016;Levi et al.,2022).The prevalence of hyperintensity cerebral ischemic lesions detected by diffusion-weighted magnetic resonance imaging(DW-MRI)was reported to be about 80%,which is associated with impaired neurocognitive function and vascular dementia(Vermeer et al.,2003;Barber et al.,2008;Kahlert et al.,2010). | Xianbao LIU Hanyi DAI Jiaqi FAN Dao ZHOU Gangjie ZHU Abuduwufuer YIDILISI Jun CHEN Yeming XU Lihan WANG Jian'an WANG | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，6： | 0 |
| 11 | Exploring Brain Age Calculation Models Available for Alzheimer's Disease显示文摘The advantages of structural magnetic resonance imaging(sMRI)-based multidimensional tensor morphological features in brain disease research are the high sensitivity and resolution of sMRI to comprehensively capture the key structural information and quantify the structural deformation.However,its direct application to regression analysis of high-dimensional small-sample data for brain age prediction may cause“dimensional catastrophe”.Therefore,this paper develops a brain age prediction method for high-dimensional small-sample data based on sMRI multidimensional morphological features and constructs brain age gap estimation(BrainAGE)biomarkers to quantify abnormal aging of key subcortical structures by extracting subcortical structural features for brain age prediction,which can then establish statistical analysis models to help diagnose Alzheimer’s disease and monitor health conditions,intervening at the preclinical stage. | Lihan Wang Honghong Liu Weijia Liu Qunxi Dong Bin Hu | 2023 | Journal of Beijing Institute of Technology2023，32，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员