| 1 | Morphological properties of medial amygdala-projecting retinal ganglion cells in the Mongolian gerbil显示文摘The amygdala is a limbic structure that is involved in many brain functions, including emotion, learning and memory. It has been reported that melanopsin-expressing retinal ganglion cells(ip RGCs) innervate the medial amygdala(Me A). However, whether conventional RGCs(c RGCs) project to the Me A remains unknown. The goal of this study was to determine if c RGCs project to the Me A and to determine the morphological properties of Me A-projecting RGCs(Me A-RGCs). Retrogradely labeled RGCs in whole-mount retinas were intracellularly injected to reveal their dendritic morphologies. Immunohistochemical staining was performed to selectively label ip RGCs(Me A-ip RGCs) and c RGCs(Me A-c RGCs). The results showed that 95.7% of the retrogradely labeled cells were c RGCs and that the rest were ip RGCs. Specifically, Me A-c RGCs consist of two morphological types. The majority of them exhibit small but dense dendritic fields and diffuse ramification patterns as previously reported in RG_(B2)(95%), while the rest exhibit small but sparse dendritic branching patterns resembling those of RG_(B3) cells(5%). Me Aip RGCs consist of M1 and M2 subtypes. The Me A-RGCs showed an even retinal distribution patterns. The soma and dendritic field sizes of the Me A-RGCs did not vary with eccentricity. In conclusion, the present results suggest that Me A-RGCs are structurally heterogeneous. These direct RGCs that input to the Me A could be important for regulating amygdala functions. | Liju Luan Chaoran Ren Wenyao Wang Yan Nan Jie Gao Mingliang Pu | 2018 | Science China(Life Sciences)2018，61，6： | 2 |
| 2 | Expression of bone morphogenetic protein 7 in the cerebral cortex of rats after ischemic-hypoxic injury显示文摘BACKGROUND:Some researches demonstrate that exogenous bone morphogenetic protein 7(BMP-7) can protect ischemic cerebral nerve tissue and promote recovery of motor energy function;however,there is lack of direct evidences of endogenous BMP-7 effect. OBJECTIVE:To observe the expression of endogenous BMP-7 in nerve tissue with ischemic-hypoxic injury and investigate the possible effects on damaged nerve tissue. DESIGN:Observational contrast animal study. SETTING:Department of Anatomy and Histoembryology,Peking University Health Science Center. MATERIALS:The experiment was carried out in the Nerve Researching Laboratory of Anatomy Department,Peking University Health Science Center from October 2006 to March 2007. A total of 25 adult male SD rats weighing 250-300 g and several newborn SD rats were selected from Experimental Animal Center,Peking University Health Science Center. Rabbit-anti-BMP-7 polyclonal antibody was provided by Wuhan Boster Company. METHODS:① Adult rats were randomly divided into ischemia group(n =10),sham operation group(n = 10) and normal group(n =5). Right external-internal carotid artery occlusion was used to infarct middle cerebral artery of adult rats in the ischemia group so as to copy focal cerebral infarction models. Line cork was inserted in crotch of internal and external carotid artery of adult rats in the sham operation group,while adult rats in the normal group were not given any treatments. ② Cerebral cortex of newborn rats was separated to obtain cell suspension. Cells which were cultured for 10 days were divided into control group and hypoxia/reoxygenation group. And then,cells in the hypoxia/reoxygenation group were cultured in hypoxic incubator for 4 hours and given reoxygenation for 24 hours. MAIN OUTCOME MEASURES:Immunohistochemical method was used to measure expression of BMP-7 in cerebral cortex at 24 hours after ischemia/reperfusion culture and in primary hypoxic culture. RESULTS:① At 24 hours after cerebral ischemia,expression of BMP-7 in cerebral cortex on ischemic side was stronger than that on non-ischemic side in adult rats;meanwhile,numbers of cell expression were increased. However,expression of BMP-7 was not detected in bilateral cerebral cortex of adult rats in both control group and sham operation group. ② After hypoxia of cerebral cortex in primary culture,positive products of BMP-7 were observed in plasma of neuron,but expression of BMP-7 was not found in normal cerebral cortex. CONCLUSION:Endogenous BMP-7 has protective effects on nerve tissue induced by ischemic-hypoxic injury. | Liju Luan Xiaomei Yang Lihua Qin Ke Wang Chunhua Chen Weiguang Zhang Lu Wan Dan Li | 2007 | Neural Regeneration Research2007，2，11： | 0 |
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