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| 1 | A Proximal Promoter Region of Arabidopsis DREB2C Confers Tissue-specific Expression under Heat Stress显示文摘The dehydration-responsive element-binding factor 2C (DREB2C) is a member of the CBF/DREB subfamily of proteins, which contains a single APETALA2/Ethylene responsive element-binding factor (AP2/ERF) domain. To identify the expression pattern of the DREB2C gene, which contains multiple transcription cis-regulatory elements in its promoter, an approximately 1.4 kb upstream DREB2C sequence was fused to the β-glucuronidase reporter gene (GUS) and the recombinant p1244 construct was transformed into Arabidopsis thaliana (L.) Heynh. The promoter of the gene directed prominent GUS activity in the vasculature in diverse young dividing tissues. Upon applying heat stress (HS), GUS staining was also enhanced in the vasculature of the growing tissues. Analysis of a series of 5 -deletions of the DREB2C promoter revealed that a proximal upstream sequence sufficient for the tissue-specific spatial and temporal induction of GUS expression by HS is localized in the promoter region between 204 and 34 bps relative to the transcriptional start site. Furthermore, electrophoretic mobility shift assay (EMSA) demonstrated that nuclear protein binding activities specific to a 120 to 32 bp promoter fragment increased after HS. These results indicate that the TATA-proximal region and some latent trans-acting factors may cooperate in HS-induced activation of the Arabidopsis DREB2C promoter. | Jihyun Je Chieun Song Jung Eun Hwang Chae Oh Lim | 2012 | Journal of Integrative Plant Biology2012，54，9： | 4 |
| 2 | Development of novel gene carrier using modified nano hydroxyapatite derived from equine bone for osteogenic differentiation of dental pulp stem cells显示文摘Hydroxyapatite(HA)is a representative substance that induces bone regeneration.Our research team extracted nanohydroxyapatite(EH)from natural resources,especially equine bones,and developed it as a molecular biological tool.Polyethylenimine(PEI)was used to coat the EH to develop a gene carrier.To verify that PEI is well coated in the EH,we first observed the morphology and dispersity of PEI-coated EH(pEH)by electron microscopy.The pEH particles were well distributed,while only the EH particles were not distributed and aggregated.Then,the existence of nitrogen elements of PEI on the surface of the pEH was confirmed by EDS,calcium concentration measurement and fourier transform infrared spectroscopy(FT-IR).Additionally,the pEH was confirmed to have a more positive charge than the 25 kD PEI by comparing the zeta potentials.As a result of pGL3 transfection,pEH was better able to transport genes to cells than 25 kD PEI.After verification as a gene carrier for pEH,we induced osteogenic differentiation of DPSCs by loading the BMP-2 gene in pEH(BMP-2/pEH)and delivering it to the cells.As a result,it was confirmed that osteogenic differentiation was promoted by showing that the expression of osteopontin(OPN),osteocalcin(OCN),and runt-related transcription factor 2(RUNX2)was significantly increased in the group treated with BMP-2/pEH.In conclusion,we have not only developed a novel nonviral gene carrier that is better performing and less toxic than 25 kD PEI by modifying natural HA(the agricultural byproduct)but also proved that bone differentiation can be effectively promoted by delivering BMP-2 with pEH to stem cells. | Myung Chul Lee Hoon Seonwoo Kyoung Je Jang Shambhavi Pandey Jaewoon Lim Sangbae Park Jae Eun Kim Yun-Hoon Choung Pankaj Garg Jong Hoon Chung | 2021 | Bioactive Materials2021，6，9： | 2 |
| 3 | Prognostic model to predict survival fol-lowing first-line chemotherapy in patients with metastatic gastric ade-nocarcinoma显示文摘 | Lee J Lim T Uhm JE | | 0，，05： | 1 |
| 4 | Use of neuron specific enolase for assessing the severity and outcome in patients with neu- rological disorders显示文摘 | Lims JE Takayanagui OM Garcia LV | 2004 | Braz J Med Biol Res2004，37，1： | 1 |
| 5 | Radiation-induced graft polymerization of glycidyl methacrylate onto nonwoven polypropylene显示文摘 | Bondar Yu V Hong Je Kim Yong Jin Lim | 2005 | Russian Journal of Applied Chemistry2005，78，5： | 1 |
| 6 | Ovarian cancer during pregnan-cy:clinical and pregnancy outcome显示文摘 | Kwon YS Mok JE Lim KT | 2010 | J Korean Med Sci2010，25，2： | 1 |
| 7 | Anti-inflammatory mechanism of simvastatin in mouse allergic asthma model显示文摘 | Kim DY Ryu SY Lim JE Lee YS Ro JY | | 0，，1： | 1 |
| 8 | TLR4 mutation reduces microglialactivation, increases Abeta deposits and exacerbates cognitivedeficits in a mouse model of Alzheimer's disease 显示文摘 | Song M Jin J Lim JE | 2011 | JNeuroinflammation2011，8，： | 1 |
| 9 | Prognostic factors following curative resection for pancreatic adenocarcinoma: a population-based, 1 inked database analysis of 396 patients 显示文摘 | Lim JE Chien MW Earle CC | 2003 | Ann Surg2003，237，： | 1 |
| 10 | The size exclusion characteristics of type I collagen: implications for the role of noncollagenous bone constituents in mineralization显示文摘 | Toroian D Lim JE Price PA | 2007 | J Biol Chem2007，282，22： | 1 |
| 11 | Performance of the tuberculin skin test and interferon-γ release assay for detection of tuberculosis infection in immunocompromised patients显示文摘 | Kim EY Lim JE Jung JY | | 0，，09： | 1 |
| 12 | Coagulation proteins influ- encing glohaI coagulation assays in cirrhosis= hypercoagulability in cirrhosis assessed by thromhomodulin induced thrombin genera- tion assay显示文摘 | Youngwon N Kim JE Lim HS | 2013 | Biomed Res Int2013，13，1： | 1 |
| 13 | Scheuermann kyphosis:safe and effective surgical treatment using multisegmental instrumentation显示文摘 | Lim M Green DW Billinghurst JE | 2004 | Spine2004，29，16： | 1 |
| 14 | A review of sudden unexpected death in the young in British Columbia显示文摘 | Lim Z Gibbs K Potts JE | 2010 | Can J Cardiol2010，26，1： | 1 |
| 15 | Individualized diabetes nutrition education improves compliance with diet prescription显示文摘 | Lim H M Park JE Choi YJ | 2009 | Nutr Res Pract2009，3，4： | 1 |
| 16 | Identification of three novel genetic variations associated with electrocardiographic traits (QRS duration and PRinterval) in East Asians显示文摘 | Hong KW Lim JE Kim JW | 2014 | Hum Mol Genet2014，23，24： | 1 |
| 17 | Evidence for multiple species of Sunda colugo显示文摘 | Janecka JE Helgen KM Lim NT Baba M Izawa M Boeadi Murphy WJ | | 0，，： | 1 |
| 18 | Questionable role of interferon-γ assays for smear-negative pulmonary TB in immunocompromised patients显示文摘 | Jung JY Lim JE Lee HJ | | 0，，02： | 1 |
| 19 | Ovarian cancer during pregnan- cy: clinical and pregnancy outcome 显示文摘 | Kwon Y Mok JE Lim KT | 2010 | J Korean Med Sci2010，25，2： | 1 |
| 20 | Arterial hyperintensity on BLADE fluid-attenuated inversion recovery images (FLAIR) in hyperacute territorial infarction: comparison with conventional FLAIR 显示文摘 | Kwag E Lim SM Park JE | 2014 | Eur Radiol2014，24，9： | 1 |
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