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| 1 | Helicobacter pylori genotype and polymorphisms in DNA repair en- zymes : where do they correlate in gastric cancer 显示文摘 | Silva - Fernandea I J da Silva TA Agnez - lima LF | 2012 | J Surg Oncol2012，106，4： | 1 |
| 2 | Self-perception and oral health in pregnant adolescents显示文摘 | Rovida TA Moimaz SA Lima DP | 2014 | Oral Health Dent Manag2014，13，3： | 1 |
| 3 | Differentiation and polypeptide expression in human papilla and dermal fibroblasts in vitro显示文摘 | Francz PI Bayreuther K Lima TA | 1993 | Eur J Cell Biol1993，60，2： | 1 |
| 4 | C-reactive protein, APOE genotype and longitudinal cognitive change in an older population显示文摘 | Lima TA Adler AL Minett T | 2014 | Age Ageing2014，43，2： | 1 |
| 5 | Proinflammatory Cytokines Correlate with Depression and Anxiety in Colorectal Cancer Patients显示文摘 | Oliveira Miranda D Soares de Lima TA Ribeiro Azevedo L et al | 2014 | Biomed Res Int2014，，： | 1 |
| 6 | Importance of radiological studies by means of computed tomography for managing fractures of the tibial plateau 显示文摘 | de Lima Lopes C da Rocha Candido Filho CA de Lima E Silva TA et d | 2014 | Rev Bras Ortop2014，49，6： | 1 |
| 7 | Full-length ax- on regeneration in the adult mouse optic nerve and partial recovery of simple visual behaviors显示文摘 | De Lima S Koriyama Y Kurimoto TA | 2012 | Proc Nat1 Acad Sci U S A2012，109，23： | 1 |
| 8 | Piwi like RNA-mediated gene silencing 1 gene as a possible major player in gastric cancer显示文摘AIM To establish a permanent piwi like RNA-mediated genesilencing 1(PIWIL1) gene knockout in AGP01 gastric cancer cell line using CRISPR-Cas9 system and analyze phenotypic modifications as well as gene expression alterations.METHODS CRISPR-Cas9 system used was purchased from Dharmacon GE Life Sciences(Lafayette, CO, United States) and permanent knockout was performed according to manufacturer's recommendations. Woundhealing assay was performed to investigate the effect of PIWIL1 knockout on migration capability of cells and Boyden chamber invasion assay was performed to investigate the effect on invasion capability. For the gene expression analysis, a one-color microarray-based gene expression analysis kit(Agilent Technologies, Santa Clara, CA, United States) was used according to the protocol provided by the manufacturer. RESULTS PIWIL1 gene knockout caused a significant decrease in AGP01 migration capacity as well as a significant decrease in cell invasiveness. Moreover, functional analysis based on grouping of all differentially expressed m RNAs identified a total of 35 genes(5 up-regulated and 30 down-regulated) encoding proteins involved in cellular invasion and migration. According to current literature, 9 of these 35 genes(DOCK2, ZNF503, PDE4 D, ABL1, ABL2, LPAR1, SMAD2, WASF3 and DACH1) are possibly related to the mechanisms used by PIWIL1 to promote carcinogenic effects related to migration and invasion, since their functions are consistent with the changes observed(being up-or down-regulated after knockout). CONCLUSION Taken together, these data reinforce the idea that PIWIL1 plays a crucial role in the signaling pathway of gastric cancer, regulating several genes involved in migration and invasion processes; therefore, its use as a therapeutic target may generate promising results in the treatment of gastric cancer. | Taíssa Araújo Andre Khayat Luciana Quintana Danielle Calcagno Ronald Mourao Antonio Modesto Juliana Paiva Adhara Lima Fabiano Moreira Edivaldo Oliveira Michel Souza Moneeb Othman Thomas Liehr Eliana Abdelhay Renata Gomes Sidney Santos Paulo Assumpcao | 2018 | World Journal of Gastroenterology2018，24，47： | 1 |
| 9 | Vaccine adjuvant:it nlakes the difference显示文摘 | Lima KM Dos santus TA Rodrigues JM | 2004 | Vaccine2004，22，19： | 1 |
| 10 | Evaluation of efficacy, safety and tolerability rabeprazole in treatment of acidpeptic diseases显示文摘 | de FREITAS TA LIMA LM RANIERI JL | 2002 | Arq Gastro-enterol2002，39，1： | 1 |
| 11 | Vaccine adjuvant: it makes the difference 显示文摘 | Lima KM dos Santos TA Rodrigues JM Jr | 2004 | Vaccine2004，22，19： | 1 |
| 12 | Identification of IL11RA and MELK amplification in gastric cancer by comprehensive genomic profiling of gastric cancer cell lines显示文摘AIM To identify common copy number alterations on gastric cancer cell lines.METHODS Four gastric cancer cell lines(ACP02, ACP03, AGP01 and PG100) underwent chromosomal comparative genome hybridization and array comparative genome hybridization. We also confirmed the results by fluorescence in situ hybridization analysis using the bacterial artificial chromosome clone and quantitative real time PCR analysis.RESULTS The amplification of 9p13.3 was detected in all cell lines by both methodologies. An increase in the copy number of 9p13.3 was also confirmed by fluorescence in situ hybridization analysis. Moreover, the interleukin 11 receptor alpha(IL11RA) and maternal embryonic leucine zipper kinase(MELK) genes, which are present in the 9p13.3 amplicon, revealed gains of the MELK gene in all the cell lines studied. Additionally, a gain in the copy number of IL11 RA and MELK was observed in 19.1%(13/68) and 55.9%(38/68) of primary gastric adenocarcinoma samples, respectively. CONCLUSION The characterization of a small gain region at 9p13.3 in gastric cancer cell lines and primary gastric adenocarcinoma samples has revealed MELK as a candidate target gene that is possibly related to the development of gastric cancer. | Danielle Queiroz Calcagno Sylvia Santomi Takeno Carolina Oliveira Gigek Mariana Ferreira Leal Fernanda Wisnieski Elizabeth Suchi Chen Taíssa Maíra Thomaz Araújo Eleonidas Moura Lima Maria Isabel Melaragno Samia Demachki Paulo Pimentel Assumpcao Rommel Rodriguez Burbano Marília Cardoso Smith | 2016 | World Journal of Gastroenterology2016，22，43： | 0 |
| 13 | Self-reported participation restrictions in normal hearing individuals in thyroid ontogeny: Evidence of subclinical changes显示文摘Purpose: To verify evidence of subclinical alterations through self-reported participation restrictions in normal hearing individuals and congenital hypothyroidism patients.Methods: An analytical, quantitative, cross-sectional exploratory study with a 1:1 ratio, consisting of a convenience sample of 86 normal hearing individuals with(n = 42) and without(n = 44) congenital hypothyroidism diagnosis. All participants underwent tonal and speech audiometry, immitance and distortion product otoacoustic emissions. The researchers excluded people with hearing loss, genetic syndromes and metabolic diseases. The instrument used for evaluate of self-reported participation restrictions was the HHIE-adapted questionnaire, composed of 25 questions, 12 of which were social domain and 13 emotional domain. Student's ttest and chi-square test were used for statistical analysis at a significance level of 5%.Results: There was a significant(p < 0.001) self-reported participation restrictions in CH(61.9%), with a greater relevance for the social domain(p = 0.002). There was a greater frequency of mild/moderate(40.5%)and higher prevalence of association with clinical factors and adherence to treatment.Conclusion: The findings indicate that self-reported participation restrictions in normal hearing individuals with congenital hypothyroidism was more significant than in the non-exposed group, suggesting evidence of subclinical auditory abnormalities in this population. | Caio Le?nidas Oliveira de Andrade Gabriela Carvalho Machado Luan Paulo Franco Magalh?es Elen Pereira de Jesus Elaine Alves Matias Taíse Lima de Oliveira Cerqueira Hélida Braga de Oliveira Luciene da Cruz Fernandes Helton Estrela Ramos Crésio de Arag?o Dantas Alves | 2019 | Journal of Otology2019，14，1： | 0 |
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