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| 1 | CIK cells from patients with HCC possess strong cytotoxicity to multidrug-resistant cell line Bel-7402/R显示文摘AIM: To investigate the cytotoxicity of the cytokine-induced killer (CIK) cells from the post-operation patients with primary hepatocellular carcinoma (HCC) to multidrugresistant (MDR) cell of HCC bothin vitro and in vivo. METHODS: A drug-resistant cell line was established by culturing human HCC cell line Bel-7402 in complete RPMI 1640 medium with increasing concentrations of adriamycin from 10 to 2 000 nmol/L. CIK cells were obtained by inducing the peripheral blood mononuclear cells with rhIFN-γ, monoclonal anti-CD3 antibody, rhIL-1α as well as rhIL-2, which were added into the culture. To detect the cytotoxicityof the CIK cells from HCC patients, the Bel-7402/R was taken as target (T) cells and CIK cells as effect (E) cells. Cytotoxic test was performed and measured by MTT. Asto in vivo test, CIK cells were transfused into patients with HCC. The tumor specimens of the patients were obtained and immunohistochemistry was carried out to detect CD3, CD45, CD45RO as well as CD68. RESULTS: A MDR 1 HCC cell line Bel-7402/R was established. Its MDR1 mRNA overexpressed which was shown by RT PCR; the P-glycoprotein expression increased from 1.32%of parent cells to 54%. CIK cells expanded vigorously bymore than 70-fold and the CD3+CD56+ increased by more than 600-fold after 3-wk incubation on average. The cytotoxicity of CIK from HCC patients to Bel-7402/R was about 50% and to L-02 below 10% (t = 8.87, P<0.01),the same as that of CIK from normal individuals. Each of the 17 patients received 1-5×1010 of CIK celltransfusion. No side effects were observed. After CIK treatment, the tumor tissue nodules formed and a large amount of lymphocytes infiltrated in the liver cancer tissue and CD3, CD45, CD45RO, and CD68 increased greatly which was shown by immunohistochemistry.CONCLUSION: A stable MDR1 HCC cell line has been established which could recover from liquid nitrogen and CIK from HCC patients has strong cytotoxicity to MDR HCC cell. CIK adoptive immunotherapy is safe and has no side effects. Receivers improved their immunity to tumor evidently. CIK treatment may be a better choice for HCC patients after operation to prevent the recurrence, especially when tumors have developed drug resistance. | You-ShunZhang Fang-JunYuan Guo-FengJia Ji-FaZhang Li-YiHu LingHuang Zong-QingDai | 2005 | World Journal of Gastroenterology2005，11，22： | 24 |
| 2 | Independent and combined effects of alcohol intake, tobacco smoking and betel quid chewing on the risk of esophageal cancer in Taiwan显示文摘 | Chien‐HungLee Jang‐MingLee Deng‐ChyangWu Hon‐KiHsu Ein‐LongKao Hsiao‐LingHuang Tsu‐NaiWang Meng‐ChuanHuang Ming‐TsangWu | 2004 | Int. J. Cancer2004，，3： | 1 |
| 3 | Empirical Tests of Efficiency Wage Models显示文摘 | Tzu‐LingHuang ArnemsHallam Peter F.Orazem Eizabeth M.Paterno | 2003 | Economica2003，，257： | 1 |
| 4 | Serological prevalence of echinococcosis and risk factors for infection among children in rural communities of southern Ningxia, China显示文摘 | Yu RongYang Philip S.Craig Dominique A.Vuitton Gail M.Williams TaoSun Tian XiLiu BelgeesBoufana PatrickGiraudoux JingTeng YanbinLi LingHuang WeiZhang Malcolm K.Jones Donald P.McManus | 2008 | Tropical Medicine & International Health2008，，8： | 1 |
| 5 | PA28β regulates cell invasion of gastric cancer via modulating the expression of chloride intracellular channel 1显示文摘 | Da‐LiZheng Qing‐LingHuang FeiZhou Qiao‐JiaHuang Jian‐YinLin XuLin | 2012 | J Cell Biochem2012，，5： | 1 |
| 6 | Identification of Different Cobalt Nucleation on Glassy Carbon显示文摘The nucleation mechanisms of cobalt from sulfate solutions were studied by utilizing the electrochemical technique, chronoamperometry. It was found that the recorded current-time transients introduced from 1.0mol/L CoSO4 solution were complexes with unusual shapes. All characteristic features were identified as separate process. The instantaneous or progressive nucleation with 2D or 3D growth exists during the cobalt deposition, depending on the applied potentials. | MinGU FangZuYANG LingHUANG ShiBingYAO ShaoMinZHOU | 2004 | Chinese Chemical Letters2004，15，8： | 0 |
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