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1篇 您的检索式：作者名="Lingjiang MIN"

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		题名	作者	年代	出处	被引量
	1	Nuclear transfer of goat somatic cells transgenic for human lactoferrin gene显示文摘Transgenic animal mammary gland bioreactors are used to produce recombinant proteins with appropriate post-translational modifications.The nuclear transfer of transgenic somatic cells is a powerful method to produce mammary gland bioreactors.We established an efficient gene transfer and nuclear transfer approach in goat somatic cells.Gene targeting vector pGBC2LF was constructed by cloning human lactoferrin(LF)gene cDNA into exon 2 of the milk goat beta-casein gene and the endogenous start codon was replaced by that of human LF gene.Goat fetal fibroblasts were transfected with lin-earized pGBC2LF and 14 cell lines were positive accord-ing to PCR and Southern blot.The transgenic cells were used as donor cells of nuclear transfer and some of recon-structed embryos could develop into blastocyst in vitro.	Lan LI Wei SHEN Lingjiang MIN Qingyu PAN Yujiang SUN Jixian DENG Qingjie PAN	2008	Frontiers in Biology2008，3，3：	0

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