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| 1 | 5-Methylcytosine RNA Methylation in Arabidopsis Thaliana显示文摘5-Methylcytosine (m 5 C ) 是描绘得好的 DNA 修正,并且主要也在初核质和优核质在丰富的非编码的 RNA 被报导。然而,分发和 m 5 在植物 mRNAs 的 C 仍然保持大部分未知。这里,我们报导 RNA m 5 在由使用 m 5 C RNA immunoprecipitation 由一条深定序的途径列在后面(m 5 C-RIP-seq ) 。LC-MS/MS 和点污点分析揭示 m 5 在各种各样的纸巾并且在不同发展阶段的 C mRNA 修正。m 5 C-RIP-seq 分析识别了 6045 m 5 在 4465 的 C 山峰在年轻幼苗表示了基因。我们发现那 m 5 C 在编码序列,二座山峰在开始 codons 以后并且在站 codons 前立即定位了被充实,并且随低翻译与 mRNAs 被联系活动。我们进一步表明了那 RNA (cytosine-5 )-methyltransferase,tRNA 特定的 methyltransferase 4B (TRM4B ) ,展览 m 5 C RNA methyltransferase 活动。在 TRM4B 的变化在根开发和减少的 m 5 C 山峰。TRM4B 影响涉及根开发的基因的抄本层次,它断然与他们的 mRNA 稳定性和 m 5 C 层次。我们的结果建议那 m 5 在 mRNA 的 C 是新 epitranscriptome 标记 inArabidopsis,并且这修正的那条规定是基因的不可分的部分规章的网络位于 \O 下面植物开发。 | Xuean Cui Zhe Liang Lisha Shen Qian Zhang Shengjie Bao Yuke Geng Bin Zhang Vonny Leo Leah A. Vardy Tiegang Lu Xiaofeng Gu Hao Yu | 2017 | Molecular Plant2017，10，11： | 19 |
| 2 | PTCH mutations in sporadic and Gorlin-syndrome-related odontogenic keratocysts 显示文摘 | Gu Xiaomei Zhao Hongshan Sun Lisha | 2006 | J Dent Res2006，85，9： | 1 |
| 3 | FSIP1 enhances the therapeutic sensitivity to CDK4/6 inhibitors in triple-negative breast cancer patients by activating the Nanog pathway显示文摘CDK4/6 inhibitors are routinely recommended agents for the treatment of advanced HR+HER2-breast cancer.However,their therapeutic effectiveness in triple-negative breast cancer(TNBC)remains controversial.Here,we observed that the expression level of fibrous sheath interacting protein 1(FSIP1)could predict the treatment response of TNBC to CDK4/6 inhibitors.High FSIP1 expression level was related to a poor prognosis in TNBC,which was associated with the ability of FSIP1 to promote tumor cell proliferation.FSIP1 downregulation led to slowed tumor growth and reduced lung metastasis in TNBC.FSIP1knockout caused cell cycle arrest at the G0/G1 phase and reduced treatment sensitivity to CDK4/6 inhibitors by inactivating the Nanog/CCND1/CDK4/6 pathway.FSIP1 could form a complex with Nanog,protecting it from ubiquitination and degradation,which may facilitate the rapid cell cycle transition from G0/G1 to S phase and exhibit enhanced sensitivity to CDK4/6 inhibitors.Our findings suggest that TNBC patients with high FSIP1 expression levels may be suitable candidates for CDK4/6 inhibitor treatment. | Guanglei Chen Lisha Sun Xi Gu Liping Ai Jie Yang Zhan Zhang Pengjie Hou Yining Wang Xunyan Ou Xiaofan Jiang Xinbo Qiao Qingtian Ma Nan Niu Jinqi Xue Hao Zhang Yongliang Yang Caigang Liu | 2023 | Science China(Life Sciences)2023，66，12： | 1 |
| 4 | A microcomputed tomographicstudy of canal isthmuses in the mesial root of mandibular first mo-lars in a Chinese population显示文摘 | Lisha Gu Xi Wei Junqi Ling | 2009 | J Endod2009，35，3： | 1 |
| 5 | A microcomputed tomographic study of canal isthmu-- ses in the mesial root of mandibular first molars in a Chinese population 显示文摘 | Lisha Gu Xi Wei Junqi Ling | 2009 | J Endod2009，35，： | 1 |
| 6 | PTCH mutations in nonsyndromic and Gorlin syndrome related odontogenic keratocysts显示文摘 | Gu Xiaomei Zhao Hongshan Sun Lisha | 2006 | J Dent Res2006，85，： | 1 |
| 7 | Relaxin-encapsulated polymeric metformin nanoparticles remodel tumor immune microenvironment by reducing CAFs for efficient triple-negative breast cancer immunotherapy显示文摘Cancer-associated fibroblasts(CAFs)are one of the most abundant stromal cells in the tumor microenvironment which mediate desmoplastic response and are the primary driver for an immunosuppressive microenvironment,leading to the failure of triple-negative breast cancer(TNBC)immunotherapy.Therefore,depleting CAFs may enhance the effect of immunotherapy(such as PD-L1 antibody).Relaxin(RLN)has been demonstrated to significantly improve transforming growth factor-β(TGF-β)induced CAFs activation and tumor immunosuppressive microenvironment.However,the short half-life and systemic vasodilation of RLN limit its in vivo efficacy.Here,plasmid encoding relaxin(pRLN)to locally express RLN was delivered with a new positively charged polymer named polymeric metformin(PolyMet),which could increase gene transfer efficiency significantly and have low toxicity that have been certified by our lab before.In order to improve the stability of pRLN in vivo,this complex was further formed lipid poly-γ-glutamic acid(PGA)/PolyMetpRLN nanoparticle(LPPR).The particle size of LPPR was 205.5±2.9 nm,and the zeta potential was+55.4±1.6 mV.LPPR displayed excellent tumor penetrating efficacy and weaken proliferation of CAFs in 4T1luc/CAFs tumor spheres in vitro.In vivo,it could reverse aberrantly activated CAFs by decreasing the expression of profibrogenic cytokine and remove the physical barrier to reshape the tumor stromal microenvironment,which enabled a 2.2-fold increase in cytotoxic T cell infiltration within the tumor and a decrease in immunosuppressive cells infiltration.Thus,LPPR was observed retarded tumor growth by itself in the 4T1 tumor bearing-mouse,and the reshaped immune microenvironment further led to facilitate antitumor effect when it combined with PD-L1 antibody(aPD-L1).Altogether,this study presented a novel therapeutic approach against tumor stroma using LPPR to achieve a combination regimen with immune checkpoint blockade therapy against the desmoplastic TNBC model. | Hongyan Zhang Liying Chen Yue Zhao Ningchao Luo Jingbin Shi Shujun Xu Lisha Ma Menglin Wang Mancang Gu Chaofeng Mu Yang Xiong | 2023 | Asian Journal of Pharmaceutical Sciences2023，18，2： | 1 |
| 8 | Single-cell analyses reveal cannabidiol rewires tumor microenvironment via inhibiting alternative activation of macrophage and synergizes with anti-PD-1 in colon cancer显示文摘Colorectal tumors often create an immunosuppressive microenvironment that prevents them from responding to immunotherapy.Cannabidiol(CBD)is a non-psychoactive natural active ingredient from the cannabis plant that has various pharmacological effects,including neuroprotective,antiemetic,anti-inflammatory,and antineoplastic activities.This study aimed to elucidate the specific anticancer mechanism of CBD by single-cell RNA sequencing(scRNA-seq)and single-cell ATAC sequencing(scATAC-seq)technologies.Here,we report that CBD inhibits colorectal cancer progression by modulating the suppressive tumor microenvironment(TME).Our single-cell transcriptome and ATAC sequencing results showed that CBD suppressed M2-like macrophages and promoted M1-like macrophages in tumors both in strength and quantity.Furthermore,CBD significantly enhanced the interaction between M1-like macrophages and tumor cells and restored the intrinsic anti-tumor properties of macrophages,thereby preventing tumor progression.Mechanistically,CBD altered the metabolic pattern of macrophages and related anti-tumor signaling pathways.We found that CBD inhibited the alternative activation of macrophages and shifted the metabolic process from oxidative phosphorylation and fatty acid oxidation to glycolysis by inhibiting the phosphatidylinositol 3-kinase-protein kinase B signaling pathway and related downstream target genes.Furthermore,CBD-mediated macrophage plasticity enhanced the response to anti-programmed cell death protein-1(PD-1)immunotherapy in xenografted mice.Taken together,we provide new insights into the anti-tumor effects of CBD. | Xiaofan Sun Lisha Zhou Yi Wang Guoliang Deng Xinran Cao Bowen Ke Xiaoqi Wu Yanhong Gu Haibo Cheng Qiang Xu Qianming Du Hongqi Chen Yang Sun | 2023 | Journal of Pharmaceutical Analysis2023，13，7： | 0 |
| 9 | Patternable and transferable silver nanowire conductors via plasma-enhanced cryo-transferring process towards highly stretchable and transparent capacitive touch sensor array显示文摘Stretchable transparent electrode(STE)plays a key role in numerous emerging applications as an indispensable component for future stretchable devices.The embedded STE,as a promising candidate,possesses balanced performances and facile preparation procedures.However,it still suffers from the defects of conductive materials caused by the transferring,which results in the irreversible failure of devices.In this work,a patternable silver nanowire(AgNW)STE was fabricated by a plasma-enhanced cryo-transferring(PEC-transferring)process.Owing to the plasma-induced sintering,the AgNW network obtained remarkable improvement in robustness,which ensured the intact network after transferring and thus led to superior tensile electrical properties of the STE.Furthermore,serpentine patterns were utilized to optimize the tensile electrical properties of the STE,which achieved a figure of merit of 292.8 and 150%resistance changing under 50%strain.As a practical application,a 4×3 array of the mutual-capacitive type stretchable touch sensors was demonstrated for future touch sensors in stretchable devices.The PEC-transferring process opened a new avenue for patternable embedded STEs and exhibited its high potential in wearable electronics and the Internet of Thing devices. | Yifan Gu Zhiguang Qiu Simu Zhu Hao Lu Lisha Peng Gaofan Zhang Ziyi Wu Xuchun Gui Zong Qin Bo-ru Yang | 2023 | Nano Research2023，16，8： | 0 |
| 10 | Immunomodulatory and Antiviral Therapy Improved Functional Cure Rate in CHB Patients with High HBsAg Level Experienced NA显示文摘Background and Aims:A functional cure,or hepatitis B virus(HBV)surface antigen(HBsAg)loss,is difficult to achieve in patients with hepatitis B virus e antigen(HBeAg)-positive chronic hepatitis B.The HBV vaccine and granulocyte-macrophage colony-stimulating factor(GM-CSF)have been reported to help reduce HBsAg levels and promote HBsAg loss.In this prospective randomized trial,we evaluated HBsAg loss in patients receiving pegylated interferon α2b(PEGIFN-α2b)and tenofovir disoproxil fumarate(TDF),with and without GM-CSF and HBV vaccination.Methods:A total of 287 patients with HBeAg positive chronic hepati-tis B and seroconversion after nucleot(s)ide analog treat-ment were assigned randomly to three treatment groups for 48 weeks,TDF alone(control),PEGIFN-α2b+TDF,and PEGIFN-α2b+TDF+GM-CSF+HBV vaccine.The prima-ry endpoints were the proportions of patients with HBsAg loss and seroconversion at 48 and 72 weeks.Resu/ts:The cumulative HBsAg loss rates in the control,PEGIFN-α2b+TDF,and PEGIFN-α2b+TDF+GM-CSF+HBV vaccine groups at week 48 were 0.0%,28.3%,and 41.1%,respec-tively.The cumulative HBsAg seroconversion rates in these groups at week 48 were 0.0%,21.7%,and 33.9%,respec-tively.Multivariate regression analysis showed that GM-CSF use plus HBV vaccination was significantly associated with HBsAg loss(p=0.017)and seroconversion(p=0.030).Con-clusions:In patients with HBeAg-positive chronic hepatitis B and seroconversion after nucleot(s)ide analog treatment,immunomodulatory/antiviral treatment regimens effective-ly improved HBsAg loss,and the regimen including GM-CSF and HBV vaccination was most effective. | Hongyu Jia Guodong Yu Jiong Yu Xiaoli Zhang Lisha Yang Bin Wang Jiming Zhang Lang Bai Xinxin Zhang Kai Wang Ping Zhao Dongliang Yang Yingren Zhao Yanyan Yu Yimin Zhang Jueqing Gu Chanyuan Ye Huan Cai Yingfeng Lu Dairong Xiang Liang Yu Jiangshan Lian Jianhua Hu Shanyan Zhang Ciliang Jin Yida Yang | 2023 | Journal of Clinical and Translational Hepatology2023，11，5： | 0 |
| 11 | Diagnostic accuracy and safety of Dermatophagoides pteronyssinus extracts used for skin prick test显示文摘Background:Dermatophagoides pteronyssinus is a common allergen causing allergic diseases in China.The aim of this study was to evaluate the efficacy and safety of D.pteronyssinus extracts produced by Peking Union Medical College Hospital(PUMCH)for the skin prick test(SPT)in the diagnosis of D.pteronyssinus allergy.Methods:A total of 910 subjects with allergic diseases were prescribed D.pteronyssinus SPT and specific sIgE(sIgE)test among the Outpatients of Department of Allergy,PUMCH from August 10,2015 to August 30,2017.Receiver operating characteristic curve(ROC)analysis was performed according to the results of D.pteronyssinus-sIgE detection.The accuracy of D.pteronyssinus extracts used for SPT in the diagnosis of D.pteronyssinus allergy was evaluated under different cutoff values.Adverse events after SPT were recorded to evaluate safety.Results:There were 796 and 618 subjects in the full analysis set(FAS)and the per protocol set(PPS),respectively.The areas under the curve of FAS and PPS were 0.871 and 0.873,respectively.According to the ROC of PPS,the optimal and 95%specificity diagnostic cutoff values of D.pteronyssinus SPT mean wheal diameter were 3.25 and 3.75 mm,respectively.No adverse events occurred.Conclusion:The extracts of D.pteronyssinus for SPT were simple,highly accurate,and safe and should be considered for recommendation in the clinical diagnosis of D.pteronyssinus allergy. | Rui Tang Xiaohong Lyu Yuxi Liu Ruiqi Wang Lianglu Wang Hong Li Jinlyu Sun Yuxiang Zhi Jianqing Gu Kai Guan Liping Wen Zixi Wang Lisha Li Le Cui Yingyang Xu Junxiong Zhou Tao Xu Jia Yin | 2022 | Chinese Medical Journal2022，135，21： | 0 |
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