|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Features versus context: an approach for precise and detailed detection and delinea- tion of faces and facial features显示文摘 | Ding Liya Martinez A M | 2010 | IEEE Transactions on Pattern Analysis and Machine Intelligence2010，32，11： | 1 |
| 2 | Total mercury and methylmercury and selenium in mercury polluted areas in Guizhou Province,China显示文摘 | Horvat M Nolde N Fajon V Jereb V Logar M Lojen S Jacimovic R Falnoga I Qu Liya Faganeli J DrobneD | 2003 | Science of Total Environment2003，304，： | 1 |
| 3 | Features versus context:an approach for precise and detailed detection and delineation of faces and facial features显示文摘 | DING Liya MARTINEZ A M | | 0，，11： | 1 |
| 4 | Features versus context: an approach for precise and detailed detection and delineation of faces and facial features 显示文摘 | Ding Liya Martinez A M | 2010 | IEEE Transactions On Pattern Analysis and Machine Intelligence2010，38，11： | 1 |
| 5 | Degradatlon of paracetamol in aqueous solutions by TiO2 photocatalysis显示文摘 | YANG Liming YU Liya RAY M | 2008 | Water Research2008，42，13： | 1 |
| 6 | Charac-terization of coal tar organics via gravity flow column chromatography显示文摘 | Liya E Y Lynn M H John D Stephen N | 1998 | Fuel1998，77，5： | 1 |
| 7 | Roles of mismatch repair proteins hMSH2 and hMLH1 in the development of sporadic breast cancer显示文摘 | Hiroaki M Nada HK Liya G | 2005 | Cancer Lett2005，223，1： | 1 |
| 8 | Reciprocity between membranous and nuclear expression of β-catenin in coIrecral tumors显示文摘 | Xing PH Tonlinson I Liyas M | 1997 | Virchows Arch1997，431，： | 1 |
| 9 | The effect of varying the lolution treatment temperature,natural aging treatment and artificial aging treatment on the mechanical strength of 7249 aluminium alloy 显示文摘 | Iskandar M Reyes D Gaxiola Y Liya M | 2002 | Materials Science Forum2002，39，6402： | 1 |
| 10 | Effects of ethylene removal on retaining freshness of imported avcado, papaya and mango fruits under modified atmosphere packages at normal temperature显示文摘 | Xiao Liya Kiyota M Xiao L | 2002 | Environment Control in Biology2002，40，1： | 1 |
| 11 | Connective tissue growth factor differentially binds to members of the cystine knot superfamily and potentiates platelet-derived growth factor-B signaling in rabbit corneal fibroblast cells显示文摘AIM:To study the binding of connective tissue growth factor(CTGF) to cystine knot-containing ligands and how this impacts platelet-derived growth factor(PDGF)-B signaling. METHODS:The binding strengths of CTGF to cystine knot-containing growth factors including vascular en-dothelial growth factor(VEGF)-A,PDGF-B,bone morphogenetic protein(BMP)-4,and transforming growth factor(TGF)-β1 were compared using the LexA-based yeast two-hybrid system. EYG48 reporter strain that carried a wild-type LEU2 gene under the control of Lex A operators and a lac Z reporter plasmid(p80plac Z) containing eight high affinity Lex A binding sites were used in the yeast two-hybrid analysis. Interactions between CTGF and the tested growth factors were evaluated based on growth of transformed yeast cells on selective media and colorimetric detection in a liquid β-galactosidase activity assay. Dissociation constants of CTGF to VEGF-A isoform 165 or PDGF-BB homo-dimer were measured in surface plasma resonance(SPR) analysis. CTGF regulation in PDGF-B presentation to the PDGF receptor β(PDGFRβ) was also quantitatively assessed by the SPR analysis. Combinational effects of CTGF protein and PDGF-BB on activation of PDGFRβ and downstream signaling molecules ERK1/2 and AKT were assessed in rabbit corneal fibroblast cells by Western analysis. RESULTS:In the LexA-based yeast two-hybrid system,cystine knot motifs of tested growth factors were fused to the activation domain of the transcriptional factor GAL4 while CTGF was fused to the DNA binding domain of the bacterial repressor protein Lex A. Yeast cotransformants containing corresponding fusion proteins for CTGF and all four tested cystine knot motifs survived on selective medium containing galactose and raffinose but lacking histidine,tryptophan,and uracil. In liquid β-galactosidase assays,CTGF expressing cells that were co-transformed with the cystine knot of VEGF-A had the highest activity,at 29.88 ± 0.91 fold above controls(P < 0.01). Cells containing the cystine knot of BMP-4 expressed the second most activity,with a 24.77 ± 0.47 fold increase(P < 0.01). Cells that contained the cystine knot of TGF-β1 had a 3.80 ± 0.66 fold increase(P < 0.05) and the ones with the cystine knot of PDGF-B had a 2.64 ± 0.33 fold increase of β-galactosidase activity(P < 0.01). Further SPR analysis showed that the association rate between VEGF-A 165 and CTGF was faster than PDGF-BB and CTGF. The calculated dissociation constant(KD) of CTGF to VEGF165 and PDGF-BB was 1.8 and 43 nmol/L respectively. PDGFBB ligand and PDGFRβ receptor formed a stable complex with a low dissociation constant 1.4 nmol/L. Increasing the concentration of CTGF up to 263.2 nmol/L significantly the ligand/receptor binding. In addition,CTGF potentiated phosphorylation of PDGFRβ and AKT in rabbit corneal fibroblast cells stimulated by PDGFBB in tissue culture condition. In contrast,CTGF did not affect PDGF-B induced phosphorylation of ERK1/2.CONCLUSION:CTGF has a differential binding affinity to VEGF-A,PDGF-B,BMP-4,and TGF-β. Its weak association with PDGF-B may represent a novel mechanism to enhance PDGF-B signaling. | Liya Pi Pei-Yu Chung Sriniwas Sriram Masmudur M Rahman Wen-Yuan Song Edward W Scott Bryon E Petersen Gregory S Schultz | 2015 | World Journal of Biological Chemistry2015，6，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员