|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Suitable internal control genes for qRT-PCR normalization in cotton fiber development and somatic embryogenesis显示文摘The mechanisms of cotton fiber development and somatic embryogenesis have been explored sys-tematically with microarray and suppression subtractive hybridization. Real-time RT-PCR provides the simultaneous measurement of gene expression in many different samples,with which the data from microarray or others can be confirmed in detail. To achieve accurate and reliable gene expression re-sults,normalization of real-time PCR data against one or several internal control genes is required,which should not fluctuate in different tissues during various stages of development. We assessed the gene expression of 7 frequently used housekeeping genes,including 18S rRNA,Histone3,UBQ7,Actin,Cyclophilin,Gbpolyubiquitin-1 and Gbpolyubiquitin-2,in a diverse set of 21 cotton samples. For fiber developmental series the expression of all housekeeping genes had the same down tendency after 17 DPA. But the expression of the AGP gene(arabinogalactan protein) that has high expression level at the later fiber development stage was up-regulated from 15 to 27 DPA. So the relative absolute quanti-fication should be an efficient and convenient method for the fiber developmental series. The expres-sion of nonfiber tissues series varied not so much against the fiber developmental series. And three best control genes Histone3,UBQ7 and Gbpolyubiquitin-1 have to be used in a combinated way to get better normalization. | TU LiLi ZHANG XianLong LIU DiQiu JIN ShuangXia CAO JingLin ZHU LongFu DENG FengLin TAN JiaFu ZHANG CunBin | 2007 | Chinese Science Bulletin2007，52，22： | 10 |
| 2 | Suppression of the homeobox gene HDTF_1 enhances resistance to Verticillium dahliae and Botrytis cinerea in cotton显示文摘Development of pathogen-resistant crops, such as fungus-resistant cotton, has significantly reduced chemical application and improved crop yield and quality. However, the mechanism of resistance to cotton pathogens such as Verticillium dahliae is still poorly understood. In this study,we characterized a cotton gene(HDTF1) that was isolated following transcriptome profiling during the resistance response of cotton to V. dahliae. HDTF1 putatively encodes a homeodomain transcription factor, and its expression was found to be down-regulated in cotton upon inoculation with V. dahliae and Botrytis cinerea. To characterise the involvement of HDTF1 in the response to these pathogens, we used virusinduced gene silencing(VIGS) to generate HDTF1-silenced cotton. VIGS reduction in HDTF1 expression significantly enhanced cotton plant resistance to both pathogens. HDTF1 silencing resulted in activation of jasmonic acid(JA)-mediated signaling and JA accumulation. However, the silenced plants were not altered in the accumulation of salicylic acid(SA) or the expression of marker genes associated with SA signaling. These results suggest that HDTF1 is a negative regulator of the JA pathway, and resistance to V. dahliae and B. cinerea can be engineered by activation of JA signaling. | Wei Gao Lu Long Li Xu Keith Lindsey Xianlong Zhang Longfu Zhu | 2016 | Journal of Integrative Plant Biology2016，58，5： | 5 |
| 3 | Visible Kerr comb generation in a high-Q silica microdisk resonator with a large wedge angle显示文摘This paper describes the specially designed geometry of a dry-etched large-wedge-angle silica microdisk resonator that enables anomalous dispersion in the 780 nm wavelength regime. This anomalous dispersion occurs naturally without the use of a mode-hybridization technique to control the geometrical dispersion. By fabricating a1-μm-thick silica microdisk with a wedge angle as large as 56° and an optical Q-factor larger than 107, we achieve a visible Kerr comb that covers the wavelength interval of 700–897 nm. The wide optical frequency range and the closeness to the clock transition at 698 nm of 87 Sr atoms make our visible comb a potentially useful tool in optical atomic clock applications. | Jiyang Ma Longfu Xiao Jiaxin Gu Hao Li Xinyu Cheng Guangqiang He Xiaoshun Jiang Min Xiao | 2019 | Photonics Research2019，7，5： | 5 |
| 4 | Effect of methotrexate combined with Sanhuang Yilong decoction on serum and synovial fluid aquaporin levels in rheumatoid arthritis dampness-heat blockage syndrome显示文摘OBJECTIVE:To study the effect of methotrexate(MTX) combined with Sanhuang Yilong decoction(SYD) on aquaporin(AQP) expression,and to explore the role of AQPs in the pathogenesis of rheumatoid arthritis(RA).METHODS:A total of 118 dampness-heat blockage type RA patients who were hospitalized in the General Chengdu Military Hospital between January2014 and December 2016 were selected as subjects in this study(30 patient of these patients with knee joint effusion were assigned to the RA synovial fluid group).For the pre-treatment controlgroups,30 healthy volunteers were recruited as the healthy control group and 30 osteoarthritis(OA) patients with knee joint effusion were included as OA synovial fluid control group.The RA dampnessheat blockage syndrome treatment groups were divided into 45 cases in the combined group and 45 cases in the MTX group.The combined group received MTX combined with SYD treatment while the MTX group received MTX alone.AQP1,AQP2 and AQP3 expressions were detected in the serum and synovial fluid.RESULTS:AQP1 had the highest expression,followed by AQP3,and AQP2.The serum levels of AQP1,AQP2 and AQP3 were all significantly lower than those in the healthy volunteers(P < 0.05),while the synovial fluid AQP1,AQP2 and AQP3 expression in the RA group were comparable to these in the OA groups(P > 0.05).After treatment for2 weeks,serum AQP1,AQP2,AQP3 were significantly increased and erythrocyte sedimentation rate,C-reactive protein,disease activity score of 28 joints were decreased in the combined group(P < 0.05).CONCLUSION:Abnormal expression of AQPs inhibits water metabolism in RA dampness-heat blockage syndrome,so liquid is accumulated at the joint,which may play an important role in the pathogenesis of RA.MTX combined with SYD for the treatment of RA can effectively increase AQP expression. | Liu Defang Yan Jiao Guo Mingyang Luo Yong Yang Mei Yang Min Zou Longfu Li Wan Zhong Xiaolong Liu Tao Hu Yonghe | 2018 | Journal of Traditional Chinese Medicine2018，38，4： | 4 |
| 5 | Nickel and indium core-shell co-catalysts loaded silicon nanowire arrays for efficient photoelectrocatalytic reduction of CO_(2) to formate显示文摘Developing an efficient artificial photosynthetic system for transforming carbon dioxide and storing solar energy in the form of chemical bonds is one of the greatest challenges in modern chemistry.However,the limited choice of catalysts with wide light absorption range,long-term stability and excellent selectivity for CO_(2) reduction makes the process sluggish.Here,a core-shell-structured nonnoble-metal Ni@In co-catalyst loaded p-type silicon nanowire arrays(SiNWs)for efficient CO_(2) reduction to formate is demonstrated.The formation rate and Faradaic efficiency of formate over the Ni@In/SiNWs catalyst reach 58μmol h^(-1) cm^(-2) and 87% under the irradiation of one simulated sunlight(AM 1.5 G,100 mW cm^(-2)),respectively,which are about 24 and 12 times those over the pristine SiNWs.The enhanced photoelectrocatalytic performance for CO_(2) reduction is attributed to the rational combination of Ni capable of effectively extracting the photogenerated electrons and In responsible for the selective activation of CO_(2). | Wenchao Ma Mingcan Xie Shunji Xie Longfu Wei Yichen Cai Qinghong Zhang Ye Wang | 2021 | Journal of Energy Chemistry2021，30，3： | 3 |
| 6 | Characteristics Analysis of the Operational Parameters of a Novel Rectifier Transformer With Filters显示文摘新型整流变压器及其滤波系统由网侧、阀侧绕组,独立滤波绕组及与之相连的滤波器组构成,其特殊的滤波支路能够在滤波和无功补偿的同时改善变压器的运行特性。首先推导以谐波抑制因子为表达方式的谐波电流抑制模型以考察谐波传递情况;然后构建网侧绕组电流和负载电流之间的数学关系,藉此研究无功补偿特性,并分析相关的运行参数;之后建立阀侧电压与网侧电压的数学关系,以研究滤波器对阀侧电压的影响。理论分析表明,新型整流变压器及其滤波系统可以有效抑制谐波,降低网侧电流和视在功率,提高网侧功率因数并增加负载电压。实际应用在工业直流供电系统中的新型整流变压器的现场实测结果,验证了理论分析的正确性。该文更全面地揭示了新型整流变压器能改善整流系统的运行特性,所推导的数学模型将为新型整流变压器在降噪节能等方面的进一步研究提供理论依据。 | ZHAO Zhiyu LUO Longfu XU Jiazhu TRAN Thanhngoc ZHANG Zhiwen | 2012 | 中国电机工程学报2012，32，22： | 2 |
| 7 | Study on characteristic parameters of a new converter transformer for HVDC sys- tems 显示文摘 | LI Yong LUO Longfu REHTANZ C | 2009 | IEEE Transactions on Power Delivery2009，24，4： | 1 |
| 8 | Down regulation of cotton GbTRP1 leads to accumulation of anthranilates and confers resistance to Verticillium dahliae显示文摘Background:Verticillium wilt,caused by Verticillium dahliae,is called a 'cancer' disease of cotton.The discovery and identification of defense-related genes is essential for the breeding of Verticillium wilt-resistant varieties.In previous research we identified some possible broad-spectrum resistance genes.Here,we report a tryptophan synthesis-related gene GbTRP1 and its functional analysis in relation to the resistance of cotton to V.dahliae.Results:Expression analysis shows that GbTRP1 is suppressed at 1 h and 6 h post V.dahliae infection,but activated at 12 h and 24 h,and the expression of GbTRP1 is highly induced by treatment with salicylic acid and jasmonic acid.Sub-cellular localization studies show that GbTRP1 is localized in the chloroplast.Suppression of GbTRP1 expression leads to lesion-mimic phenotypes and activates the immune response in cotton by showing enhanced resistance to V.dahliae and B.cinerea.Metabolomic analysis shows that anthranilic compounds significantly accumulated in GbTRP1-silenced plants,and these metabolites can inhibit the growth of V.dahliae and B.cinerea in vitro.Conclusions:Our results show that suppression of GbTRPI expression dramatically activates the immune response and increases resistance of cotton to V.dahliae and B.cinerea,possibly due to the accumulation of anthranilate compounds.This study not only provides genetic resources for disease resistance breeding,but also may provide a basis for new chemical control methods for combatting of fungal disease in cotton. | MIAO Yuhuan ZHU Longfu ZHANG Xianlong | 2019 | Journal of Cotton Research2019，2，3： | 1 |
| 9 | Soft-Fauh Diagnosis of Analog Circuit with Tolerance Using FNLP显示文摘 | Zhang Wei Shi Yibing Zhou Longfu | 2010 | Poland:Metrology and Measurement Systems2010，17，5： | 1 |
| 10 | Operation charac- teristics of a new HVDC transmission system显示文摘 | LUO Longfu ZENG Jinhui XU Jiazhu | 2012 | Advanced Science Letters2012，11，1： | 1 |
| 11 | A new converter transformer and a corresponding inductive filtering method for HVDC transmission system显示文摘 | Luo Longfu Li Yong Xu Jiazhu | 2008 | IEEE Trans on Power Delivery2008，23，3： | 1 |
| 12 | Analog Circuit Fault Diagnosis Using AdaBoost and SVM 显示文摘 | Jingyuan Tang Yibing Shi Longfu Zhou | 2008 | International Conference on Communications Circuits and Systems Fujian China2008，2008，： | 1 |
| 13 | A new converter transformer and a corresponding inductive filtering method for HVDC transmission system 显示文摘 | LUO Longfu LI Yong XU Jiazhu | 2008 | IEEE Trans on Power Delivery2008，23，3： | 1 |
| 14 | Identification of candidate genes controlling fiber quality traits in upland cotton through integration of meta-QTL,significant SNP and transcriptomic data显示文摘Background:Meta-analysis of quantitative trait locus(QTL)is a computational technique to identify consensus QTL and refine QTL positions on the consensus map from multiple mapping studies.The combination of meta-QTL intervals,significant SNPs and transcriptome analysis has been widely used to identify candidate genes in various plants.Results:In our study,884 QTLs associated with cotton fiber quality traits from 12 studies were used for meta-QTL analysis based on reference genome TM-1,as a result,74 meta-QTLs were identified,including 19 meta-QTLs for fiber length;18 meta-QTLs for fiber strength;11 meta-QTLs for fiber uniformity;11 meta-QTLs for fiber elongation;and 15 meta-QTLs for micronaire.Combined with 8589 significant single nucleotide polymorphisms associated with fiber quality traits collected from 15 studies,297 candidate genes were identified in the meta-QTL intervals,20 of which showed high expression levels specifically in the developing fibers.According to the function annotations,some of the 20 key candidate genes are associated with the fiber development.Conclusions:This study provides not only stable QTLs used for marker-assisted selection,but also candidate genes to uncover the molecular mechanisms for cotton fiber development. | XU Shudi PAN Zhenyuan YIN Feifan YANG Qingyong LIN Zhongxu WEN Tianwang ZHU Longfu ZHANG Dawei NIE Xinhui | 2020 | Journal of Cotton Research2020，3，4： | 1 |
| 15 | Partheno-genetic Algorithm and Its Application显示文摘 | Li Maojun Tong Tiaosheng Luo Longfu | 1998 | Journal of Hunan University1998，25，6： | 1 |
| 16 | A new converter trans- former and a corresponding inductive filtering method :or HVDC transmission system显示文摘 | Luo Longfu Li Yong Xu Jiazhu | 2008 | IEEE Transactions on Power Delivery2008，23，3： | 1 |
| 17 | Parameter Selection, Analysis and Evaluation of an Improved Particle Swarm Optimizer with Lead- ership显示文摘 | Longfu Zhou Yibing Shi Yanjun Li | 2010 | Artificial Intelligence Review2010，34，4： | 1 |
| 18 | Partheno-Genetic Algorithm and Its Application显示文摘 | Tong Tiaosheng Luo Longfu | 1998 | Journal of Huana Univ1998，25，6： | 1 |
| 19 | Parameter selection,analysis and evaluation of an improved particle swarm optimizer with leadership显示文摘 | Longfu Zhou Yibing Shi Yanjun Li | 2010 | Artificial Intelligence Review2010，34，： | 1 |
| 20 | Enhancement of commutation reliability of an HVDC inverter by means of an inductive filtering method显示文摘 | Li Yong Liu Fang Luo Longfu | 2013 | IEEE Transactions on Power Electronics2013，28，11： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员