|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Intravenous vs intraperitoneal mesenchymal stem cells administration:What is the best route for treating experimental colitis?显示文摘AIM:To investigate the therapeutic effects of mesenchymal stem cells(MSCs)transplanted intraperitoneally andintravenously in a murine model of colitis.METHODS:MSCs were isolated from C57BL/6 mouse adipose tissue.MSC cultures were analyzed according to morphology,cellular differentiation potential,and surface molecular markers.Experimental acute colitis was induced in C57BL/6 mice by oral administration of2%dextran sulfate sodium(DSS)in drinking water ad libitum from days 0 to 7.Colitis mice were treated with1×106 MSCs via intraperitoneal or intravenous injection on days 2 and 5.The disease activity index was determined daily based on the following parameters:weight loss,stool consistency and presence of blood in the feces and anus.To compare morphological and functional differences in tissue regeneration between different MSC injection modalities,mice were euthanized on day 8,and their colons were examined for length,weight,and histopathological changes.Inflammatory responses were determined by measuring the levels of different serum cytokines using a CBA Th1/Th2/Th17 kit.Apoptotic rates were evaluated by terminal deoxynucleotidyl transferase-mediated d UDPbiotin nick end labeling assay.RESULTS:Intravenous infusion of MSCs was more effective than intraperitoneal treatment(P<0.001)in reducing the clinical and histopathologic severity of colitis,which includes weight loss,diarrhea and inflammation.An histological evaluation demonstrated decreased colonic inflammation based on reduced crypt loss and reduced infiltration of inflammatory cells.This therapeutic effect was most likely mediated by the down-regulation of pro-inflammatory cytokines[interleukin(IL)-6 and tumor necrosis factor(TNF)];and by the up-regulation of anti-inflammatory cytokines(IL-10 and IL-4).Intravenous transplantation alsoinduced high levels of IFN that lead to activation of the immunosuppressive activity of the MSCs,which did not occur with intraperitoneal transplantation(P=0.006).An increase in apoptotic T cells was observed after intravenous,but not intraperitoneal,MSC infusion,suggesting that MSCs can induce apoptosis in resistant T cells in colonic inflammation(P=0.027).CONCLUSION:Our results demonstrate that intravenous treatment is a superior method for reducing colon inflammation compared with intraperitoneal therapy. | Fabiany da Costa Goncalves Natália Schneider Fernanda Otesbelgue Pinto Fabíola Schons Meyer Fernanda Visioli Bianca Pfaffenseller Patrícia Luciana da Costa Lopez Eduardo Pandolfi Passos Elizabeth Obino Cirne-Lima Luíse Meurer Ana Helena Paz | 2014 | World Journal of Gastroenterology2014，20，48： | 4 |
| 2 | Patient reports of satisfaction after microvascular decompression and partial sensory rhizotomy for trigeminal neuralgia 显示文摘 | Zakrzewska JM Lopez BC Kim SE | 2005 | Neurosurgery2005，56，6： | 1 |
| 3 | Patient reports of satisfaction after microvascular decompression and partial sensory rhizotomy for trigeminal neuralgia显示文摘 | Zakrzewska JM Lopez BC Kim SE | 2005 | Neurosurg2005，56，13： | 1 |
| 4 | Peritoneal tuberculosis: report of seven cases 显示文摘 | Flores-Alvarez E Tello-Brand SE Lopez Lopez F | 2010 | Cirugia y Cirujanos2010，78，1： | 1 |
| 5 | Actuaral outcome after catheter ballooncommissurotomy in patients with mitral stenosis显示文摘 | ORANGE SE KOWANISHI DT LOPEZ BM | 1997 | Circulation1997，95，： | 1 |
| 6 | Patient reports of satisfaction after microvascular decompression and partial sensory rhizotomy for trige-minal neuralgia显示文摘 | Zakrzewska JM Lopez BC Kim SE | 2005 | Neurosurgery2005，56，6： | 1 |
| 7 | Characterization of size-specif- ic particulate matter emission rates for a simulated medical laser pro- cedure-a pilot study显示文摘 | Lopez R Lacey SE Lippert JF | 2015 | Ann Occup Hyg2015，59，4： | 1 |
| 8 | Patient reports of satisfaction after microvascular decompression and partial sensory rhizotomy for trigeminal neuralgia显示文摘 | Zakrzewska JM Lopez BC Kim SE | 2005 | Neurosurgery2005，56，6： | 1 |
| 9 | CT scan efficiency in emergency room diagnosis for optic neuropathy due to trauma显示文摘 | Lopez SE Espana GE Frances ME | 2001 | Arch Soc Esp Oftalmol2001，76，10： | 1 |
| 10 | Patient reports of satisfaction after microvascular decompression and partial sensory rhizotomy for trigeminal neuralgia显示文摘 | Zakrzewska JM Lopez BC Kim SE | 2005 | Neurosurgery2005，56，： | 1 |
| 11 | Counseling psychology, s focus on positive aspects of human functioning显示文摘 | Lopez SJ Magyar MJI Petersen SE | 2006 | Counseling Psythologist2006，34，2： | 1 |
| 12 | Human interleukin - 3 mRNA accumulation is controlled at both the transcriptional and posttranscriptional level 显示文摘 | Ryan GR Milton SE Lopez AF | 1991 | Blood1991，77，6： | 1 |
| 13 | Actuarial outcome after catheter balloon commissurotomy in patients with mitral stenosis显示文摘 | Orrange SE Kawanishi DT Lopez BM | 1997 | Circulation1997，97，： | 1 |
| 14 | Patient reports of satisfaction after microvascular decompression and partial sensory rhizotomy for trigeminal neuralgia 显示文摘 | Zakrzewska JM Lopez BC Kim SE | 2005 | Neurosurgery2005，56，6： | 1 |
| 15 | Patient reports of satis- faction after microvascular decompression and partial sensory rhi- zotomy for trigeminal neuralgia 显示文摘 | Zakrzewska JM Lopez BC Kim SE | 2005 | Neurosurgery2005，56，6： | 1 |
| 16 | l Actuarial outcome after catheterballoon commissurotomy in patientswithmitral stenosis 显示文摘 | Orrange SE Kawanishi DT Lopez BM et a | 1997 | Circulation1997，97，： | 1 |
| 17 | Patient reports of satisfaction after microvascular decompression and partial sensory rhizotomy for trigeminal neuralgia显示文摘 | Zakrzewska JM Lopez BC Kim SE | 2005 | Neurosurgery2005，56，： | 1 |
| 18 | Genetic diversity of the IL-4, IL -4 receptor and IL - 13 loci in mestizos in the general population and in patients with asthma from three subpopulations in Mexico 显示文摘 | Lopez KI Martinez SE Moguel MC | 2007 | Int J Immunogenet2007，34，1： | 1 |
| 19 | Patient reports of satisfaction after microvascular decompression and partial sensory rhizotomy for trigeminal neuralgia显示文摘 | Zakrzewska JM Lopez BC Kim SE | | 0，，06： | 1 |
| 20 | Counseling psychology’s focus on positive aspects of human functioning显示文摘 | Lopez SJ Magyar MJL Petersen SE et a1 | 2006 | The Counseling Psycholo-gist2006，34，2： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员