|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Hepatitis B virus-induced oncogenesis显示文摘Hepatocellular carcinoma (HCC) is one of the most com- mon cancers in the world with an annual incidence of more than 500 000 in the year 2000. Its incidence is rising in many countries. Recently, it has been estimated that about 53% of HCC cases in the world are related to hepatitis B virus (HBV). The epidemiological association of HBV with HCC is well established. In recent studies, it was revealed that HBsAg carriers have a 25-37 times increased risk of developing HCC as compared to non-infected people. At present, HBV-associated carcinogenesis can be seen as a multi-factorial process that includes both direct and indirect mechanisms that might act synergistically. The integration of HBV DNA into the host genome occurs at early steps of clonal tumor expansion. The integration has been shown in a number of cases to affect a variety of cancer- related genes and to exert insertional mutagenesis. The permanent liver inflammation, induced by the immune response, resulting in a degeneration and regeneration process confers to the accumulation of critical mutations in the host genome. In addition to this, the regulatory proteins HBx and the PreS2 activators that can be encoded by the integrate exert a tumor promoter-like function resulting in positive selection of cells producing a functional regulatory protein. Gene expression profiling and proteomic techniques may help to characterize the molecular mechanisms driving HBV-associated carcino- genesis, and thus potentially identify new strategies in diagnosis and therapy. | Joachim Lupberger Eberhard Hildt | 2007 | World Journal of Gastroenterology2007，13，1： | 56 |
| 2 | Identification and characterization of a novel bipartite nuclear localization signal in the hepatitis B virus polymerase显示文摘AIM:To characterize the nuclear import of hepatitis B virus(HBV)polymerase(P)and its relevance for the viral life cycle.METHODS:Sequence analysis was performed to predict functional motives within P.Phosphorylation of P was analyzed by in vitro phosphorylation.Phosphorylation site and nuclear localization signal(NLS)were destroyed by site directed mutagenesis.Functionality of the identified NLS was analyzed by confocal fluorescence microscopy and characterizing the karyopherin binding.Relevance of the structural motives for viral life cycle was studied by infection of primary Tupaia hepatocytes with HBV.RESULTS:We identified by sequence alignment and functional experiments a conserved bipartite NLS containing a casein kinaseⅡ(CKⅡ)phosphorylation site located within the terminal protein domain(TP)of the HBV polymerase.Inhibition of CKⅡimpairs the functionality of this NLS and thereby prevents the nuclear import of the polymerase.Binding of the import factor karyopherin-α2 to the polymerase depends on its CKⅡ-mediated phosphorylation of the bipartite NLS.In HBV-infected primary Tupaia hepatocytes CKⅡinhibition in the early phase(post entry phase)of the infection process prevents the establishment of the infection.CONCLUSION:Based on these data it is suggested that during HBV infection the final import of the genome complex into the nucleus is mediated by a novel bipartite NLS localized in the TP domain of HBV polymerase. | Joachim Lupberger Stephanie Schaedler Alexander Peiran Eberhard Hildt | 2013 | World Journal of Gastroenterology2013，19，44： | 8 |
| 3 | Fluorescent 5'- exonuclease assay for the absolute quantification of Wilms' tumour gene (WT1) mRNA: implications for monitoring hu- man leukaemias显示文摘 | Kreuzer KA Saborowski A Lupberger J | 2001 | Br J Haematol2001，114，2： | 1 |
| 4 | Fluorescent 5 '-exonuclease assay for the absolute quantification of Wilms' tumour gene ( WT1 ) mRNA: implication for monitoring human leukaemias 显示文摘 | Kreuzer KA Saborowski A Lupberger J | 2001 | Br J Haematol2001，114，： | 1 |
| 5 | Virus-host Interactions during Hepatitis C Virus Entry-Implications for Pathogenesis and Novel Treatment Approaches显示文摘Hepatitis C virus (HCV) is a member of the Flaviviridae family and causes acute and chronic hepatitis. Chronic HCV infection may result in severe liver damage including liver cirrhosis and hepatocellular carcinoma. The liver is the primary target organ of HCV, and the hepatocyte is its primary target cell. Attachment of the virus to the cell surface followed by viral entry is the first step in a cascade of interactions between the virus and the target cell that is required for successful entry into the cell and initiation of infection. This step is an important determinant of tissue tropism and pathogenesis; it thus represents a major target for antiviral host cell responses, such as antibody-mediated virus neutralization. Following the development of novel cell culture models for HCV infection our understanding of the HCV entry process and mechanisms of virus neutralization has been markedly advanced. In this review we summarize recent developments in the molecular biology of viral entry and its impact on pathogenesis of HCV infection, development of novel preventive and therapeutic antiviral strategies. | Joachim Lupberger Mirjam B. Zeisel Anita Haberstroh Eva K. Schnober Sophie Krieger Eric Soulier Christine Thumann Cathy Royer Samira Fafi-Kremer Catherine Schuster Franoise Stoll-Keller Hubert E. Blum Thomas F. Baumert | 2008 | Virologica Sinica2008，23，2： | 1 |
| 6 | Quantitative RT-PCR of Wilms tumor gene transcripts(WT1) for the molecular monitoring of patients with accelerated phase bcr/abl+CML显示文摘 | Na IK Kreuzer KA Lupberger J | 2005 | Leuk Res2005，29，3： | 1 |
| 7 | EGFR and EphA2 are host factors for hepatitis C virus entry and possible targets for antiviral therapy显示文摘 | Lupberger J Zeisel MB Xiao F | 2011 | Nature Medicine2011，17，5： | 1 |
| 8 | Apolipoprotein E interacts with hepatitis C virus nonstructural protein 5A and determines assembly of infectious particles显示文摘 | Benga WJA Krieger SE Dimitrova M Zeisel MB Parnot M Lupberger J Hildt E Luo G McLauchlan J Baumert TF Schuster C | 2009 | Hepatology2009，51，1： | 1 |
| 9 | EGFR and EphA2are host factors for hepatitis C virus entry and possible targets for antiviral therapy显示文摘 | Lupberger J Zeisel M B Xiao F Thumann C Fofana I Zona L Davis C Mee C J Turek M Gorke S Royer C Fischer B Zahid M N Lavillette D Fresquet J Cosset F L Rothenberg S M Pietschmann T Patel A H Pessaux P Doffol M Raffelsbe-rger W Poch O McKeating J A Brino L Baumert T F | 2011 | Nat Med2011，17，5： | 1 |
| 10 | Hepatitis B virus-induced oncogenesis显示文摘 | Lupberger J Hildt E | 2007 | World J Gastroenterol2007，13，1： | 1 |
| 11 | Fluorescent 5'-exonuclease assay for the absolute quantification of Wilms' tumour gene (WT1) mRNA, implications for monitoring human leukemias 显示文摘 | Kreuzer KA Saborowski A Lupberger J | 2001 | Br J Haematol2001，114，2： | 1 |
| 12 | EGFR and EphA2 are host factors for hepatitis C virus entry and possible targets for antiviral therapy显示文摘 | Lupberger J Zeisel MB Xiao F | | 0，，05： | 1 |
| 13 | Quantitative RT PCR of Wilms tumor gene transcripts(WT1) for the molecular mo- nitoring of patients with accelerated phase bcr/abl + CML显示文摘 | Na IK Kreuzer KA Lupberger J | 2005 | Leuk Res2005，29，3： | 1 |
| 14 | Fluorescent 5'-exonuclease assayfor the absolute quantification of Wilm's tumour gene (WT1) mRNA:implication for monitoring human leukemias显示文摘 | Kreuzer KA Saborowski A Lupberger J | 2001 | Haematol2001，114，2： | 1 |
| 15 | Fluorescent 5'-exonuclease assay for the absolute quantification of Wilms' tumour gene (WT1) mRNA: implications for monitoring human leukaemias显示文摘 | Kreuzer KA Saborowski A Lupberger J | 2001 | Br J Haematol2001，114，2： | 1 |
| 16 | HRas Signal Transduction Promotes Hepatitis C Virus Cell Entry by Triggering Assembly of the Host Tetraspanin Receptor Complex显示文摘 | Laetitia Zona Joachim Lupberger Nazha Sidahmed-Adrar Christine Thumann Helen J. Harris Amy Barnes Jonathan Florentin Rajiv G. Tawar Fei Xiao Marine Turek Sarah C. Durand Fran?ois H.T. Duong Markus H. Heim Fran?ois-Lo?c Cosset Ivan Hirsch Didier Samuel Lau | 2013 | Cell Host & Microbe2013，，3： | 1 |
| 17 | Ralstonia eutropha H16 encodes two and possibly three intracellular po]y depolymerase genes显示文摘 | York G M Lupberger J Tian J | 2003 | Journal of Bacteriology2003，185，13： | 1 |
| 18 | Cholesterol uptake and hepatitis C virus entry显示文摘 | Joachim Lupberger Daniel J. Felmlee Thomas F. Baumert | 2012 | Journal of Hepatology2012，，1： | 1 |
| 19 | EGFR and EphA2 are host factors for hepatitis C virus entry and possible targets for antiviral therapy显示文摘 | Lupberger J Zeisel M B Xiao F | 2011 | Nat Med2011，17，5： | 1 |
| 20 | Cultivation of HepG2.2.15 on Cytodex-3: Higher yield of hepatitis B virus and less subviral particles compared to conventional culture methods显示文摘 | Joachim Lupberger Andreas Mund Josef Kock Eberhard Hildt | 2006 | Journal of Hepatology2006，，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员