|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Effect of Helicobacter pylori cdrA on interleukin-8 secretions and nuclear factor kappa B activation显示文摘AIM:To investigate genetic diversity of Helicobacter pylori (H.pylori) cell division-related gene A (cdrA) and its effect on the host response.METHODS:Inactivation of H.pylori cdrA,which is involved in cell division and morphological elongation,has a role in chronic persistent infections.Genetic property of H.pylori cdrA was evaluated using polymerase chain reaction and sequencing in 128 (77 American and 51 Japanese) clinical isolates obtained from 48 and 51 patients,respectively.Enzyme-linked immunosorbent assay was performed to measure interleukin-8 (IL-8) secretion with gastric biopsy specimens obtained from American patients colonized with cdrA-positive or-negative strains and AGS cells cocultured with wild-type HPK5 (cdrA-positive) or its derivative HPKT510 (cdrA-disruptant).Furthermore,the cytotoxin-associated gene A (cagA) status (translocation and phosphorylation) and kinetics of transcription factors [nuclear factor-kappa B (NF-κB) and inhibition kappa B] were investigated in AGS cells co-cultured with HPK5,HPKT510 and its derivative HPK5CA (cagA disruptant) by western blotting analysis with immunoprecipitation.RESULTS:Genetic diversity of the H.pylori cdrA gene demonstrated that the cdrA status segregated into two categories including four allele types,cdrA-positive (allele types;Ⅰand Ⅱ) and cdrA-negative (allele types;Ⅲ and Ⅳ) categories,respectively.Almost all Japanese isolates were cdrA-positive (Ⅰ:7.8% and Ⅱ:90.2%),whereas 16.9% of American isolates were cdrA-positive (Ⅱ) and 83.1% were cdrA-negative (Ⅲ:37.7% and Ⅳ:45.5%),indicating extended diversity of cdrA in individual American isolates.Comparison of each isolate from different regions (antrum and corpus) in the stomach of 29 Americans revealed that cdrA status was identical in both isolates from different regions in 17 cases.However,12 cases had a different cdrA allele and 6 of them exhibited a different cdrA category between two regions in the stomach.Furthermore,in 5 of the 6 cases possessing a different cdrA category,cdrA-negative isolate existed in the corpus,suggesting that cdrA-negative strain is more adaptable to colonization in the corpus.IL-8 secretions from AGS revealed that IL-8 levels induced by a cdrA-disrupted HPKT510 was significantly lower (P < 0.01) compared to wildtype HPK5:corresponding to 50%-60% of those of wild-type HPK5.These data coincided with in vivo data that an average value of IL-8 in biopsy specimens from cdrA-positive and cdrA-negative groups was 215.6 and 135.9 pg/mL,respectively.Western blotting analysis documented that HPKT510 had no effect on CagA translocation and phosphorylation,however,nuclear accumulation of NF-κB was lower by HPKT510 compared to HPK5.CONCLUSION:Colonization by a cdrA-negative or cdrA-dysfunctional strain resulted in decreased IL-8 production and repression of NF-κB,and hence,attenuate the host immunity leading to persistent infection. | Hiroaki Takeuchi Ya-Nan Zhang Dawn A Israel Richard M Peek Jr Mikio Kamioka Hideo Yanai Norihito Morimoto Tetsuro Sugiura | 2012 | World Journal of Gastroenterology2012，18，5： | 3 |
| 2 | Anticoagulant therapy for idiopathic pulmonary fibrosis显示文摘 | Kubo H Nakayama K Yanai M | | 0，，03： | 2 |
| 3 | Forest floor microbial binmass across a northern hardwood successional sequence显示文摘 | Taylor L A Arthur M A Yanai R D | | 0，，03： | 1 |
| 4 | Seasonal heating of the Tibetan Plateau and its effects on the evolution of the Asian summer monsoon显示文摘 | Yanai M Li Chengfeng Z Song | 1992 | J Meteor Soc Japan1992，70，1： | 1 |
| 5 | Correlation of proliferative and anti-apoptotic effects of HGF, insulin, IGF-1, IGF-2, and EGF in SV40-transformed human corneal epithelial cells 显示文摘 | Yanai R Yamada N Inui M | 2006 | Exp Eye Res2006，83，1： | 1 |
| 6 | Functional changes after prenatal opiate exposure related to opiate receptors' regulated aherations in cholinergic innervation 显示文摘 | Yanai J Huleihel R Izrael M | 2003 | Inter J Neuropsychopharmacolog2003，6，： | 1 |
| 7 | Tzdashi kido and Kimiye Bzba显示文摘 | TANIGUTU M YANAI M XIAO Y G | 1996 | Phytochemistry1996，42，3： | 1 |
| 8 | Anticoagulant therapy for idiopathic pulmonary fibrosis 显示文摘 | Kubo H Nakayama K Yanai M | 2005 | Chest2005，128，3： | 1 |
| 9 | The ecology of root lifespan 显示文摘 | EISSENSTAT D M YANAI R D | 1997 | Advan Ecol Res1997，27，： | 1 |
| 10 | Evolution of a tropical disturbances in the Carbean Sea region显示文摘 | YANAI M | 1968 | J Meteor Soy Japan1968，46，2： | 1 |
| 11 | The onset and interannual variability of the Asian summer monsoon in relation to land-sea thermal constrast显示文摘 | Li C F M Yanai | 1996 | J Climate1996，9，2： | 1 |
| 12 | Interpretation of the resulls of blood culture and interventional role by clinicallaboratory physicians显示文摘 | Yanai M Uehara Y Yagoshi M | 2006 | Rinsho Byori2006，54，0： | 1 |
| 13 | Seasonal heating of the Tibetan Plateau and its effects on the evolution of the Asian summer monsoon显示文摘 | Yanai M Li C Song Z | 1992 | Journal Meteorological Society of Japan1992，70，： | 1 |
| 14 | Prolyl endopeptidease inhib- itory peptides in wine 显示文摘 | Yanai T Suzuki Y Sato M | 2003 | Biosci Biotechnol Biochem2003，67，2： | 1 |
| 15 | Intervention to prevent pneumonia among old adult显示文摘 | Yamaya M Yanai M Ohrui T et at | 2001 | J Am Geriatr Soc2001，49，： | 1 |
| 16 | Trajectory Control of Incompletely Restrained Parallel-wire-suspended Mechanism Based on Inverse Dynamics显示文摘 | Yamamoto M J Yanai N Mohri A | 2004 | IEEE Transactions on Robotics2004，20，5： | 1 |
| 17 | Seasonal heating of the Tibetan Plateau and it's effects on the evolution of the Asian summer monsoon显示文摘 | Yanai M Li C Song Z S | 1992 | J Meteor Soc Japan1992，70，1： | 1 |
| 18 | Role of the C domain of IGFs in synergistic promotion,with a substance P-derived peptide,of rabbit corneal epithelial wound healing显示文摘 | Yamada N Yanai R Nakamura M | 2004 | Invest Ophthalmol Vis Sci2004，45，4： | 1 |
| 19 | Anticoagulant therapy for idiopathic pulmonary fibrosis 显示文摘 | Kubo H Nakayama K Yanai M | 2005 | Chest2005，128，3： | 1 |
| 20 | Interpretation of the results of blood culture and intcrventional role by clinical laboratory physicians显示文摘 | Yanai M Uehara Y Yagoshi M | 2006 | Rinsho Byori2006，54，10： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员