| 1 | Aquaporin-4 Mz Isoform:Brain Expression,Supramolecular Assembly and Neuromyelitis Optica Antibody Binding显示文摘水通道蛋白4(AQP4)表达于大脑和脊髓的星形胶质细胞。2种主要的AQP4亚型,M1和M23,均有表达,具有不同的翻译起始点。研究表明,一种较新的亚型Mz表达于大鼠,其翻译起始点位于M1的翻译起始点上游126 bp。通过C端标记的抗AQP4抗体SDS和非变性胶免疫印迹技术,大鼠大脑中的Mz被检测到为39 kDa大小的条带。Mz因其提前终止密码子,在人类和小鼠大脑中并不表达。通过单粒子追踪和非变性胶电泳技术检测,发现大鼠Mz可形成正交粒子阵列(OAPs)。本文发现,Mz与M1类似,在细胞浆膜内迅速扩散,并不形成OAPs。但是,当与M23共表达时,Mz通过与M23形成异四聚体可与OAPs关联。意外的是,Mz表达的细胞极弱地与视神经脊髓炎自身抗体(NMO-IgG)结合,小于M1表达细胞的5倍。切割分析提示,Met-1上游的31~41残基参与NMO-IgG与Mz之间较弱的结合。总之,Mz AQP4在大鼠中低量表达,但不表达于人和小鼠的大脑;自身无法形成OAPs,除非与M23 AQP4形成异四聚体;因AQP4/NMO-IgG结合点的N端功能。 | Andrea Rossi Jonathan M.Crane A.S.Verkman | 2011 | 神经损伤与功能重建2011，6，5： | 4 |
| 3 | Space heating and hot water demand analysis of dwellings connected to district heating scheme in UK显示文摘To achieve CO2 emissions reductions,the UK Building Regulations require developers of new residential buildings to calculate expected CO2 emissions arising from their energy consumption using a methodology such as Standard Assessment Procedure(SAP 2005) or,more recently SAP 2009.SAP encompasses all domestic heat consumption and a limited proportion of the electricity consumption.However,these calculations are rarely verified with real energy consumption and related CO2 emissions.This work presents the results of an analysis based on weekly heat demand data for more than 200 individual flats.The data were collected from a recently built residential development connected to a district heating network.A method for separating out the domestic hot water(DHW) use and space heating(SH) demand has been developed and these values are compared to the demand calculated using SAP 2005 and SAP 2009 methodologies.The analysis also shows the variation in DHW and SH consumption with size of flats and with tenure(privately owned or social housing).Evaluation of the space heating consumption also includes an estimate of the heating degree day(HDD) base temperature for each block of flats and compares this to the average base temperature calculated using the SAP 2005 methodology. | R.Burzynski M.Crane R.Yao V.M.Becerra | 2012 | Journal of Central South University2012，19，6： | 0 |
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