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| 1 | Flow cytometric detection of hepatitis C virus antigens in infected peripheral blood leukocytes: Binding and entry显示文摘AIM: We designed two synthetic-core-specific peptides core 1 (C1) and core 2 (C2), and an E1-specific peptide (E1). We produced specific polyclonal antibodies againstthese peptides and used the antibodies for detection of HCV antigens on surface and within infected peripheral blood leukocytes.METHODS: Peripheral blood from a healthy individual who tested negative for HCV RNA was incubated with HCV type 4 infected serum for 1 h and 24 h at 37 ℃. Cells were stained by direct and indirect immunofluorescence and measured by flow cytometry.RESULTS: After 1 h of incubation, antibodies against C1,C2, and E1 detected HCV antigens on the surface of 27%,26% and 73% of monocytes respectively, while 10%, 5% and 9% of lymphocytes were positive with anti-C1, anti-C2 and anti-E1 respectively. Only 1-3% of granulocytes showed positive staining with anti-C1, anti-C2 and anti E1 antibodies. After 24 h of incubation, we found no surface staining with anti-C1, anti-C2 or anti-E1. Direct immunostaining using anti-C2 could not detect intracellular HCV antigens, after 1 h of incubation with the virus, while after 24 h of incubation, 28% of infected cells showed positive staining. Only plus strand RNA was detectable intracellularly as early as 1 h after incubation, and remained detectable throughout 48 h post-infection.Interestingly, minus RNA strand could not be detected after 1 h, but became strongly detectable intracellularly after 24 h post-infection.CONCLUSION: Monocytes and lymphocytes are the preferred target cells for HCV infection in peripheral blood leukocytes. Our specific anti-core and anti-E1 antibodies are valuable reagents for demonstration of HCV cell cycle.Also, HCV is capable of infecting and replicating in peripheral blood mononuclear cells as confirmed by detection of minus strand HCV RNA as well as intracellular staining of core HCV antigen. | Mostafa K El-Awady Ashraf A Tabll El-Rashdy M Redwan Samar Youssef Moataza H Omran Fouad Thakeb Maha El-Demellawy | 2005 | World Journal of Gastroenterology2005，11，33： | 4 |
| 2 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 3 | Processing and characterization of reinforced polyethylene composites made with lignocellulosic fibers from Egyptian agro-industrial residues显示文摘 | YOUSSEF H WALEED K E Z MAHA M I | 2008 | Composites Science and Technology2008，68，： | 1 |
| 4 | Thermal testing and numerical simulation of a prototype cell using light wallboards coupling vacuum isolation panels and phase change material 显示文摘 | Maha Ahmad Bontemps A Sallee H | 2006 | Energy and Buildings2006，6，38： | 1 |
| 5 | Three cases of macrofollic- ular variant of papillary thyroid carcinoma 显示文摘 | Emad R Maha A Kfoury H K | 2011 | Ann Saudi Med2011，31，6： | 1 |
| 6 | The functional architecture for face- processing expertise:FMRl evidence of the developmental trajecto- ry of the core and the extended face systems 显示文摘 | Frank H Maha A Jarnet HW | 2013 | Neuropsychoto- gia2013，51，13： | 1 |
| 7 | Using near infrared data to classify tobacco types and identify native grown tobacco显示文摘 | MAHA H MCCLURE W F | 1997 | Near Infrared Spectros1997，，5： | 1 |
| 8 | Experimental investigation and computer simulation of thermal behaviour of wallboards containing a phase change material显示文摘 | MAHA A ANDR(E) B H(E)BERT S | 2006 | Energy and Buildings2006，38，4： | 1 |
| 9 | Her-2/neu over-expression in muscle-invasive urothelial carcinoma of the bladder: prognostic signifi- cance and comparative analysis in primary and metastatic tumors 显示文摘 | Rafael E Maha H Fernando J | 2001 | Clinical Cancer Research2001，7，： | 1 |
| 10 | Effect of network parameters on neighbor wireless link bre-aks in GPSR protocol and enhancement using mobility pre-diction model显示文摘 | READ A hlsaqour MAHA S Abdelhaq OLA h hlsukour | 2012 | EURASIP Journal on Wireless Commu-nications and Networking2012，2012，1： | 1 |
| 11 | Anomalous origin of the entire coronary system by three separate ostia within the right coronary sinus a rarely observed coronary anomaly显示文摘 | Maha AM Brett H Jonathon L | 2010 | Canadian J Cardiol2010，26，6： | 1 |
| 12 | Production of isoamyl acetate with immobilized Staphylococcus simulans lipase in a solvent-free system显示文摘 | Ghamgui H Maha K C Bezzine S | 2006 | Enzyme and Microbial Technology2006，38，6： | 1 |
| 13 | Ixabepilone (Epothilone B Analogue BMS-247550) is active in chemotherapy-naive patients with hormone refractory prostate cancer:a Southwest Oncology Group Trial S0111显示文摘 | Maha H Tangen CM Lara PN Jr | 2005 | J Clin Oncol2005，23，24： | 1 |
| 14 | Immobilization of albumin on magnetite nanoparticles 显示文摘 | Mahas E Ozmen M Vural H C | 2011 | Materials Let- ters2011，65，： | 1 |
| 15 | Global trends in research related to sleeve gastrectomy:A bibliometric and visualized study显示文摘BACKGROUND One of the most popular bariatric procedures is sleeve gastrectomy,and it has become significantly more common in recent years.AIM To evaluate the research activity in sleeve gastrectomy over the last two decades,and to visualize the hot spots and emerging trends in this type of bariatric surgeryusing bibliometric methods.METHODS The Scopus database was used to search for publications related to sleeve gastrectomy.The retrieved publications were reviewed in terms of year of publication,type of study,country of origin,institutions,journals,and citation patterns by using descriptive analysis.Collaboration network and term cooccurrence analysis were visualized by using VOSviewer software.RESULTS The search strategy yielded a total of 6508 publications on sleeve gastrectomy from 2001 to 2020.As regards the document type,the majority were articles(n=5230;80.36%),followed by reviews(n=544;8.36%).The top three countries are the United States,with 1983 publications(30.47%),followed by France(600;9.22%)and Italy(417;6.71%).The most cited publication was published in 2012 by Schauer et al in the New England Journal of Medicine(n=1435 citations).This publication found that weight loss was greater in the sleeve gastrectomy group than in the medical therapy group.Furthermore,this study demonstrated that 12 mo of medical therapy plus bariatric surgery greatly improved glycemic regulation in obese patients with uncontrolled type 2 diabetes compared with medical therapy alone.The focus of the current literature on sleeve gastrectomy was directed toward several themes such as morbidity and potential complications,the complexity of the procedure and different surgical approaches,and diabetes and body mass index in correlation to sleeve gastrectomy.CONCLUSION The number of sleeve gastrectomy publications has gradually grown over the last 20 years.This bibliometric analysis could help researchers better understand the knowledge base and research frontiers surrounding sleeve gastrectomy.In addition,future studies may focus on emerging research hotspots. | Abdulkarim Barqawi Faris AK Abushamma Maha Akkawi Samah W Al-Jabi Moyad Jamal Shahwan Ammar Abdulrahman Jairoun Sa'ed H Zyoud | 2021 | World Journal of Gastrointestinal Surgery2021，13，11： | 0 |
| 16 | Effects of methyl palmitate and lutein on LPS-induced acute lung injury in rats显示文摘AIM: To investigate the effects of methyl palmitate and lutein on lipopolysaccharide(LPS)-induced acute lung injury(ALI) in rats and explore the possible mechanisms. METHODS: Male Sprague-Dawley rats were divided into 4 groups:(1) control;(2) LPS;(3) Methyl palmitate; and(4) Lutein groups. Methyl palmitate(300 mg/kg, ip) was administered 3 times per week on alternating days while lutein(100 mg/kg, oral) was given once daily. After 1 wk of vehicle/methyl palmitate/lutein treatment, ALI was induced by a single dose of LPS(7.5 mg/kg, iv). After 24 h of LPS injection, animals were sacrificed then biochemical parameters and histopathology were assessed. RESULTS: Treatment with methyl palmitate attenuated ALI, as it significantly decreased the lung wet/dry weight(W/D) ratio, the accumulation of the inflammatory cells in the bronchoalveolar lavage fluid(BALF) andhistopathological damage. However, methyl palmitate failed to decrease lactate dehydrogenase(LDH) activity in BALF. On the other hand, lutein treatment produced significant anti-inflammatory effects as revealed by significant decrease in accumulation of inflammatory cells in lung, LDH level in BALF and histopathological damage. Methyl palmitate and lutein significantly increased superoxide dismutase(SOD) and reduced glutathione(GSH) activities with significant decrease in the lung malondialdehyde(MDA) content. Importantly, methyl palmitate and lutein decreased the level of the inflammatory cytokine tumor necrosis factor-α(TNF-α) in the lung. Lutein also reduced LPS-mediated overproduction of pulmonary nitrite/nitrate(NO-2/NO-3), which was not affected by methyl palmitate pretreatment. CONCLUSION: These results demonstrate the potent protective effects of both methyl palmitate and lutein against LPS-induced ALI in rats. These effects can be attributed to potent antioxidant activities of these agents, which suppress inflammatory cell infiltration and regulated cytokine effects. | El-Sayed M Ammar Maha H Sharawy Asem A Shalaby Dina S El-Agamy | 2013 | World Journal of Respirology2013，3，2： | 0 |
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