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| 1 | Evaluation of clinical relevance of examining K-ras, p16 and p53 mutations along with allelic losses at 9p and 18q in EUS-guided fine needle aspiration samples of patients with chronic pancreatitis and pancreatic cancer显示文摘AIM: To establish an optimum combination of molecular markers resulting in best overall diagnostic sensitivity and specificity for evaluation of suspicious pancreatic mass. METHODS: Endoscopic ultrasound (EUS)-guided fine needle aspiration cytology (FNA) was performed on 101 consecutive patients (63 males, 38 females, 60 ± 12 years; 81 with subsequently diagnosed pancreatic cancer, 20 with chronic pancreatitis) with focal pancreatic mass. Samples were evaluated on-site by an experienced cytopathologist. DNA was extracted from Giemsa stained cells selected by laser microdissection and the presence of K-ras, p53 and p16 somatic mutations was tested by cycling-gradient capillary electrophoresis (CGCE) and single-strand conformation polymorphism (SSCP) techniques. In addition, allelic losses of tumor suppressor genes p16 (INK4, CDKN2A) and DPC4 (MADH4, SMAD4) were detected by monitoring the loss of heterozygosity (LOH) at 9p and 18q, respectively. RESULTS: Sensitivity and specificity of EUS-guided FNA were 75% and 85%, positive and negative predictivevalue reached 100%. The remaining 26% samples were assigned as inconclusive. Testing of molecular markers revealed sensitivity and specificity of 70% and 100% for K-ras mutations (P < 0.001), 24% and 90% for p53 mutations (NS), 13% and 100% for p16 mutations (NS), 85% and 64% for allelic losses at 9p (P < 0.001) and 78% and 57% for allelic losses at 18q (P < 0.05). When tests for different molecular markers were combined, the best results were obtained with K-ras + LOH at 9p (92% and 64%, P < 0.001), K-ras + LOH at 18q (92% and 57%, P < 0.001), and K-ras + LOH 9q + LOH 18q (96% and 43%, P < 0.001). When the molecular markers were used as complements to FNA cytology to evaluate inconclusive samples only, the overall sensitivity of cancer detection was 100% in all patients enrolled in the study. CONCLUSION: EUS-guided FNA cytology combined with screening of K-ras mutations and allelic losses of tumor suppressors p16 and DPC4 represents a very sensitive approach in screening for pancreatic malignancy. Molecular markers may find its use particularly in cases where FNA cytology has been inconclusive. | C Salek L Benesova M Zavoral V Nosek L Kasperova M Ryska R Strnad E Traboulsi M Minarik | 2007 | World Journal of Gastroenterology2007，13，27： | 18 |
| 2 | Children With Respiratory Distress Treated With High-Flow Nasal Cannula显示文摘 | Spentzas T Minarik M Patters AB | 2009 | J Intensive Care Med2009，24，5： | 1 |
| 3 | Children with respiratory distress treated with high-flow nasal cannula显示文摘 | Spentas T Minarik M Patters AB | 2009 | J Intensive Care Med2009，24，5： | 1 |
| 4 | Contribution of whole - body magnetic resonance in the diagnostics of monoelonal gammopathy of undetermined significance, multiple myeloma, and the as- sessment of Durie - Sahnon Plus staging system 显示文摘 | Scudla V Herman M Minarik J | 2011 | Vnitr Lek2011，57，1： | 1 |
| 5 | Diagnostic imaging in the follow-up of nonoperative management of splenic trauma in children显示文摘 | Minarik L Slim M Rachlin S | 2002 | Pediatr Surg Int2002，18，56： | 1 |
| 6 | A Special Instrument for Exact Control of Self-organized Structures Preparation in Polymer Layers显示文摘 | Minarik A Perutka M Urban P | 2010 | Int J Heat Mass Tran2010，53，2324： | 1 |
| 7 | Rapid testing of clo pidogrel resistance by genotyping of CYP2C19 and CYP2C9 poly- morphisms using denaturing on-chip capillary electrophoresis显示文摘 | Minarik M Kopeckova M Gassman M | 2012 | Electrophoresis2012，33，8： | 1 |
| 8 | Rapid testing of clo-pidogrel resistance by genotyping of CYP2C19 and CYP2C9 poly-morphisms using denaturing on-chip capillary electrophoresis显示文摘 | Minarik M Kopeckova M Gassman M | 2012 | Electrophoresis2012，33，8： | 1 |
| 9 | A novel high-resolution chipCE assay for rapid detection of EGFR gene mutations and amplifications in lung cancer therapy by a combination of fragment analysis,denaturing CE and MLPA显示文摘 | Minarik M Gassman M Belsanova B | 2010 | Electrophoresis2010，31，21： | 1 |
| 10 | Malate dehydrogenases-structure and function 显示文摘 | MINARIK P TOMASKOVA N KOLLAROVA M | 2002 | Gen Physiol Biophys2002，3，： | 1 |
| 11 | Poststroke depression incidence and risk factors: an integrative literature review 显示文摘 | Johnson JL Minarik PA Nystr~m KV et 81 | 2006 | J Neumsci Nuts2006，38，4： | 1 |
| 12 | Mutation detection in KRAS Exon 1 by constant denaturant capillary electrophoresis in 96 parallel capillaries显示文摘 | Bjorheim Minarik M Gaudernack G Ekstrom PO | 2002 | Anal Biochem2002，304，2： | 1 |
| 13 | Malate dehydrogenases-structure and function显示文摘 | Minarik P Tomaskova N Kollarova M | 2002 | General Physiology and Biophysics2002，21，3： | 1 |
| 14 | 显示文摘 | MINARIK M MINARIKOVA L HRABIKOVA M | 2004 | Electrophoresis2004，25，78： | 1 |
| 15 | Post- stroke depression incidence and risk factors: an integrative literature review 显示文摘 | JOHNSON J L MINARIK P A NYSTR M K V | 2006 | The Journal of Neuroscience Nursing : Journal of the American Association of Neuroscience Nurses2006，38，4： | 1 |
| 16 | Left ventricular systolic and diastolic function in children with overweight and obesity显示文摘 | Schusterova I Jurko A Minarik M | | 0，，09： | 1 |
| 17 | Zeeman-tuned Slowing of Rubidium Usingσ+ andσ-Polarized Light显示文摘 | MAYER S K MINARIK N S SHROYER M H | 2002 | Opt Comm2002，210，： | 1 |
| 18 | Relationship between circulating tumor cells,blood coagulation,and urokinaseplasminogen-activator system in early breast cancer patients显示文摘 | Mego M Karaba M Minarik G | 2015 | Breast J2015，21，2： | 1 |
| 19 | Poststroke depression incidence and risk factors:an integrative literature review显示文摘 | Johnson JL Minarik PA Nystr(o)m KV | 2006 | J Neurosic Nurs2006，38，4： | 1 |
| 20 | Relationship between circulating tumor cells, blood coagulation, and urokinase - plasminogen- activatorsystem in early breast cancer pa- tients 显示文摘 | Mego M Karaba M Minarik G | 2015 | Breast J2015，21，2： | 1 |
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