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您的检索式：作者名="Maeva Rodriguez"
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| 1 | Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima显示文摘Nucleic acid sensing is a 3 decades old but still challenging area of application for different biological sub-domains,from pathogen detection to single cell transcriptomics analysis.The many applications of nucleic acid detection and identification are mostly carried out by PCR techniques,sequencing,and their derivatives used at large scale.However,these methods’limitations on speed,cost,complexity and specificity have motivated the development of innovative detection methods among which nucleic acid biosensing technologies seem promising.Toehold switches are a particular class of RNA sensing devices relying on a conformational switch of secondary structure induced by the pairing of the detected trigger RNA with a de novo designed synthetic sensing mRNA molecule.Here we describe a streamlined methodology enabling the development of such a sensor for the RNA-mediated detection of an endangered plant species in a cell-free reaction system.We applied this methodology to help identify the rosewood Dalbergia maritima,a highly trafficked wood,whose protection is limited by the capacity of the authorities to distinguish protected logs from other unprotected but related species.The streamlined pipeline presented in this work is a versatile framework enabling cheap and rapid development of new sensors for custom RNA detection. | Paul Soudier Daniel Rodriguez Pinzon Tristan Reif-Trauttmansdorff Hassan Hijazi Maeva Cherriere Catia Goncalves Pereira Doriane Blaise Maxime Pispisa Angelyne Saint-Julien William Hamlet Melissa Nguevo Eva Gomes Sophia Belkhelfa Anna Niarakis Manish Kushwaha Ioana Grigoras | 2022 | Synthetic and Systems Biotechnology2022，7，2： | 1 |
| 2 | Evolution of respiratory system compliance and potential for lung recruitment in COVID-19–induced acute respiratory distress syndrome显示文摘Background:Coronavirus disease 2019(COVID-19)has been frequently complicated by severe acute respiratory distress syndrome(ARDS)with prolonged invasive ventilation.While respiratory system compliance and lung recruitability have been described within the first days after ICU admission,data about their longitudinal changes are still limited.Therefore,we conducted this study to assess the evolution of respiratory system compliance and lung recruitability in patients with COVID-19–related ARDS.Method:We conducted a prospective single-center study in patients admitted for COVID-19–related ARDS during the first wave of the pandemic,from March 16,2020 to April 10,2020.Respiratory system compliance was calculated daily at clinical positive end-expiratory pressure(PEEP)during passive breathing.The potential for lung recruitment was assessed by measuring the volume derecruited between PEEP 15 cmH2 O and 5 cmH2 O,and using the calculation of the recruitment-to-inflation ratio(R/I ratio).Recruitable lung was considered when the R/I ratio was at least 0.5.The primary outcome was the evolution of respiratory mechanics over time.The secondary outcome was the evolution of lung recruitability over time.Results:Thirty-two patients were included in this study.The respiratory mechanics were assessed 222 times(7±5 times per patient).Respiratory system compliance at clinical PEEP was 29.1 mL/cmH2 O(interquartile range[IQR]:24.1–33.9 mL/cmH2 O)and decreased significantly over time(P<0.0001).Lung recruitability was assessed in 22 out of the 32 patients(60 assessments).The median volume derecruited between PEEP 15 cmH2 O and 5 cmH2 O was 246.8 mL(IQR:180.8–352.2 mL)and the median R/I ratio was 0.56(IQR:0.39–0.73).Neither changed significantly over time.The proportion of patients with recruitable lung was 50.0%(6/12)within the first 3 days after intubation,69.2%(9/13)between day 4 and day 7,and 66.7%(8/12)after day 7(P=0.7934).Conclusions:In our cohort,respiratory system compliance was low and decreased over time.The potential for lung recruitment was high and persisted despite prolonged mechanical ventilation,suggesting that maintaining high PEEP levels in the later course of COVID-19 could be adequate. | Maeva Rodriguez Sylvain Le Pape François Arrivé Jean-Pierre Frat Arnaud WThille Rémi Coudroy | 2022 | Journal of Intensive Medicine2022，2，4： | 0 |
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