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| 1 | 我国食品微生物定量风险评估的研究进展显示文摘近10余年来,我国政府重视食品安全并逐渐加强风险分析体系的构建和实施。根据历年来国家卫生与计生委的统计数据,表明由致病微生物导致的食物中毒发病率一直高于其他危害。食源性致病菌引起的食品安全风险是全球性问题,发展中国家面临的情况更为严峻,因此加强我国微生物定量风险评估以减少与发达国家的差距,从国家层面上势在必行。特别是2009年我国颁布实施《食品安全法》和2011年成立国家食品安全风险评估中心(China National Center for Food Safety Risk Assessment,CFSA)以来,已有不少针对国内具体食品致病菌情况开展的食品微生物定量风险评估(quantitative microbial risk assessment,QMRA)研究。本文对2000年至今我国已开展的QMRA研究,包括涉及的食品、致病菌、微生物预测模型、剂量-效应模型等进行详细综述。同时指出我国开展QMRA面临的技术性难题及解决方法,并对采用QMRA结果用于构建危害分析与关键控制点(Hazard Analysis and Critical Control Point,HACCP)和食品安全目标(food safety objective,FSO)以及执行目标(performance objective,PO)的应用前景进行探讨。建议加强风险评估与风险管理的互动交流、进一步完善风险监测、微生物限量制定和国际合作,在完善实施指南的基础上针对我国具体国情开展更多具有科学性和系统性的QMRA研究。 | 董庆利 王海梅 Pradeep K MALAKAR 刘箐 宋筱瑜 田明胜 陆冉冉 | 2015 | 食品科学2015，36，11： | 43 |
| 2 | Safety assessment of Bifidobacterium longum JDM301 based on complete genome sequences显示文摘AIM:To assess the safety of Bifidobacterium longum (B.longum) JDM301 based on complete genome sequences.METHODS:The complete genome sequences of JDM301 were determined using the GS 20 system.Putative virulence factors,putative antibiotic resistance genes and genes encoding enzymes responsible for harmful metabolites were identified by blast with virulence factors database,antibiotic resistance genes database and genes associated with harmful metabolites in previous reports.Minimum inhibitory concentration of 16 common antimicrobial agents was evaluated by E-test.RESULTS:JDM301 was shown to contain 36 genes associated with antibiotic resistance,5 enzymes related to harmful metabolites and 162 nonspecific virulence factors mainly associated with transcriptional regulation,adhesion,sugar and amino acid transport.B.longum JDM301 was intrinsically resistant to ciprofloxacin,amikacin,gentamicin and streptomycin and susceptible to vancomycin,amoxicillin,cephalothin,chloramphenicol,erythromycin,ampicillin,cefotaxime,rifampicin,imipenem and trimethoprim-sulphamethoxazol.JDM301 was moderately resistant to bacitracin,while an earlier study showed that bifidobacteria were susceptible to this antibiotic.A tetracycline resistance gene with the risk of transfer was found in JDM301,which needs to be experimentally validated.CONCLUSION:The safety assessment of JDM301 using information derived from complete bacterial genome will contribute to a wider and deeper insight into the safety of probiotic bacteria. | Yan-Xia Wei Zhuo-Yang Zhang Chang Liu Pradeep K Malakar Xiao-Kui Guo | 2012 | World Journal of Gastroenterology2012，18，5： | 5 |
| 3 | 气单胞菌在不同食物接触表面的存活与交叉污染显示文摘为探讨气单胞菌在不同食物接触表面的存活状况,采用擦拭取样法测定气单胞菌于案板、刀具表面的恢复率,并将气单胞菌分别接种于洁净和被污染的案板、刀具表面,评定气单胞菌在不同介质表面、不同时间下的存活状况。根据恢复率和介质表面上气单胞菌存活实测值,采用@Risk软件对介质表面实时带菌量的期望值进行计算,并运用一级线性模型对气单胞菌存活特征曲线进行拟合。同时,以案板为交叉污染途径,对气单胞菌在介质表面的存活行为对交叉污染的影响进行了探讨。结果表明:采用擦拭取样法测得的气单胞菌从案板、刀具表面的恢复率的最佳分布拟合结果分别是Normal(0.66,0.15)和Normal(0.72,0.18);气单胞菌在被污染的介质表面存活的时间(7 h)长于其在洁净介质表面的存活时间(3 h或2.5 h),且一级线性模型可以较好地对气单胞菌存活曲线进行拟合(R2>0.88);蒙特卡罗模拟结果表明,忽略气单胞菌在食物接触表面的存活行为会高估其在厨房内的交叉污染水平。 | 董庆利 王海梅 Pradeep K Malakar 陆冉冉 | 2015 | 农业机械学报2015，46，3： | 5 |
| 4 | 食源性致病菌菌株多相异质性对微生物风险评估的影响研究进展显示文摘微生物风险评估是当前保障食品安全的重要科学工具之一,被广泛应用于食品安全政策、法规和标准的制定。菌株多相异质性是微生物的固有特性,普遍存在于各类致病菌之中,影响着风险评估的准确性和不确定性。本文概述了国内外食品微生物菌株多相异质性的相关研究,总结了菌株多相异质性的概念,从生长和失活异质性、被膜形成异质性、毒力异质性和耐药异质性4 个方面全面系统地分析了菌株多相异质性的存在形式。在此基础上探讨了菌株多相异质性对微生物风险评估的影响,进一步展望了降低该影响的4 种解决措施,提出微生物风险评估宏模型的概念,以期提高食品安全风险评估的准确性和可靠性。 | 俞文英 张昭寰 刘海泉 Pradeep Kumar MALAKAR 潘迎捷 赵勇 | 2019 | 食品科学2019，40，13： | 4 |
| 5 | Handwritten and printed word identification using gray-scale feature vector and decision tree classifier显示文摘 | Malakar S Das R K Sarkar R | 2013 | Procedia Technology2013，10，： | 1 |
| 6 | Determining banana size based on computer vision显示文摘 | HU M DONG Q MALAKAR P K | 2015 | International Journal of Food Properties2015，18，3： | 1 |
| 7 | Molecular identification of three Ompok species using mitochondrial COI gene 显示文摘 | MALAKAR A K LAKRA W S GOSWAMI M | 2012 | Mitochondrial DNA2012，23，1： | 1 |
| 8 | Estimating risk from small inocula by using population growth parameters 显示文摘 | Malakar P K Barker G C | 2009 | Applied and Environmental Microbiology2009，75，19： | 1 |
| 9 | Effect of substrate and IPTG concentra- tions on the burden to growth of Escherichia eoli on glycerol due to the expression of Lae proteins 显示文摘 | Malakar P Venkatesh K V | 2012 | Appl Microbiol Biotechnol2012，93，6： | 1 |
| 10 | Protective role of S -adenosyl-L -methionine against hydrochloric acid stressin Saccharomyces cerevisiae显示文摘 | | 2006 | Biochimical et Biophysica Acta2006，1760，： | 1 |
| 11 | Repigmantation of vitiligo withpunch grafting and narrow-band UV-B(311nm)-a prospectivestudy显示文摘 | Lahiri K Malakar S Sarnia N | 2006 | Int J Dermattd2006，45，： | 1 |
| 12 | Ondansetron HC1 Microemulsions for Transdermal Delivery: Formulation and In Vitro Skin Permeation 显示文摘 | Malakar J K Nayak A Basu A | 2012 | ISRN Pharmaceutics2012，2012，： | 1 |
| 13 | Molecular identification of three Ompok species using mitochondrial COI gene显示文摘 | Abhishek K. Malakar Wazir S. Lakra M. Goswami M. Singh R. M. Mishra | 2012 | Mitochondrial DNA2012，，1： | 1 |
| 14 | Genetic polymorphism of glutathione S-transferases M1 and T1,tobacco habits and risk of stomach cancer in Mizoram,India显示文摘 | Malakar M Devi KR Phukan RK | 2012 | Asian Pac J Cancer Prev2012，13，9： | 1 |
| 15 | Surgical pearl: composite film and graft unit for the recipient area dressing after split-thickness skin grafting in vitiligo 显示文摘 | Malakar S Malakar RS | 2001 | J Am Acad Dermatol2001，44，5： | 1 |
| 16 | Modeling the interactions between Lactobacillus curvatus and Enterobacter cloacae显示文摘 | MALAKAR P K MARTENS D E ZWIETERING M H | 1999 | International Journal of Food Microbiology1999，51，1： | 1 |
| 17 | Acropigmentation of dohi in a 12-year-old boy显示文摘 | Dhar S Malakar S | | 0，，02： | 1 |
| 18 | Repigmentation of vitiligo with punch grafting and narrow-band UV-B (311 nm)-a prospective study显示文摘 | Lahiri K Malakar S Sarma N | 2006 | Int J Dermatol2006，45，6： | 1 |
| 19 | A perspective towards development and com- mercialization of potential BGA biofertilizers of Assam, North East India and carrier materials for BGA mass production and inoculum development 显示文摘 | Malakar E Kalita MC | 2012 | Annals of Biological Research2012，3，1： | 1 |
| 20 | Effect of substrate and IPTG concentrations on the burden to growth of Escherichia coli on glycerol due to the expression of Lac proteins 显示文摘 | MALAKAR P VENKATESH K V | 2012 | Ap plied Microbiology and Biotechnology2012，93，6： | 1 |
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